Galectin-9 Induces Maturation of Human Monocyte-derived Dendritic Cells
-
- YAMAUCHI Akira
- Department of Cell Regulation, Faculty of Medicine, Kagawa University
-
- DAI Shu-Yan
- Department of Immunology and Immunopathology, Faculty of Medicine, Kagawa University
-
- NAKAGAWA Ryusuke
- Department of Cell Regulation, Faculty of Medicine, Kagawa University
-
- KASHIO Yumiko
- Department of Immunology and Immunopathology, Faculty of Medicine, Kagawa University
-
- ABE Hiroko
- Department of Cell Regulation, Faculty of Medicine, Kagawa University
-
- KATOH Shigeki
- Department of Cell Regulation, Faculty of Medicine, Kagawa University
-
- KONTANI Keiichi
- Department of Second Department of Surgery, Faculty of Medicine, Kagawa University
-
- HIRASHIMA Mitsuomi
- Department of Immunology and Immunopathology, Faculty of Medicine, Kagawa University
Bibliographic Information
- Other Title
-
- 糖鎖結合蛋白ガレクチン9による樹状細胞成熟化
Search this article
Abstract
We investigated the role of galectin-9 (Gal-9) in maturation of dendritic cells (DC). Culture of immature DCs with exogenous Gal-9 markedly increased the surface expression of CD40, CD54, CD80, CD83, CD86, and HLA-DR in a concentration-dependent manner, although Gal-9 had no effect on differentiation of human monocytes into immature DCs. Gal-9-treated DCs secreted IL-12 but not IL-10, and they elicited the production of Th1 cytokines (IFN-γ and IL-2), but not that of the Th2 cytokines (IL-4 and IL-5) by allogeneic CD4+ T cells. These effects of Gal-9 on immature DCs were not essentially dependent on its lectin properties, given that they were only slightly inhibited by lactose. We further found that a Gal-9 mutant that lacks β-galactoside binding activity reproduced the above activities, and that an anti-Gal-9 mAb suppressed them. Gal-9 induced phosphorylation of the p38 MAPK and ERK1/2 in DCs, and an inhibitor of p38 signaling, but not inhibitors of signaling by either ERK1/2 or phosphatidylinositol 3-kinase, blocked Gal-9-induced up-regulation of costimulatory molecule expression and IL-12 production. These findings suggest that Gal-9 plays a role not only in innate immunity but also in acquired immunity by inducing DC maturation and promoting Th1 immune responses.<br>
Journal
-
- Japanese Journal of Clinical Immunology
-
Japanese Journal of Clinical Immunology 28 (6), 381-388, 2005
The Japan Society for Clinical Immunology
- Tweet
Details
-
- CRID
- 1390282679627449216
-
- NII Article ID
- 10020414811
-
- NII Book ID
- AN00357971
-
- ISSN
- 13497413
- 09114300
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- Crossref
- CiNii Articles
- KAKEN
-
- Abstract License Flag
- Disallowed