Identification of rule for expression and selection of engram cells
Project/Area Number |
16H04653
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Neurophysiology / General neuroscience
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Research Institution | University of Toyama |
Principal Investigator |
Ohkawa Noriaki 富山大学, 大学院医学薬学研究部(医学), 講師 (80416651)
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Research Collaborator |
INOKUCHI kaoru
FUKAI tomoki
FUNG chi chung
TAKEKAWA takashi
|
Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
|
Keywords | 記憶 / エピソード / 学習 / 記憶痕跡 / カルシウムイメージング / 海馬 / 記憶痕跡細胞 |
Outline of Final Research Achievements |
The brain stores and recalls memories through a set of neurons, termed engram cells. We established a unique imaging system that combines Ca2+ imaging and engram identification to extract the characteristics of engram activity by visualizing and discriminating between engram and non-engram cells. Here, we show that engram cells detected in the hippocampus display higher repetitive activity than non-engram cells during novel context learning. The total activity pattern of the engram cells during learning is stable across post-learning memory processing. Within a single engram population, we detected several sub-ensembles composed of neurons collectively activated during learning. Some sub-ensembles preferentially reappear during post-learning sleep, and these replayed sub-ensembles are more likely to be reactivated during retrieval. These results indicate that sub-ensembles represent distinct pieces of information, which are then orchestrated to constitute an entire memory.
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Academic Significance and Societal Importance of the Research Achievements |
本研究成果では、日々の生活で経験するエピソードの記憶について、脳内の神経細胞がどのような様式で集団で活動し記憶情報を符号化しているのかを明らかにしたとともに、経験に応じて形成された記憶が睡眠中に定着する様子を観察し明らかにした。これらの知見は、記憶の科学的かつ本質的な理解に貢献するものであり、脳内に表出した情報の解読法の提案や、効率の良い記憶学習法、認知症などの記憶障害の早期診断法への応用につながることが期待される。
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Overlapping memory trace indispensable for linking, but not recalling, individual memories.2017
Author(s)
Yokose, J., Okubo-Suzuki, R., Nomoto, M., Ohkawa, N., Nishizono, H., Suzuki, A., Matsuo, M., Tsujimura, S., Takahashi, Y., Nagase, M.,Watabe, A.M., Sasahara, M., Kato, F., Inokuchi, K.
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Journal Title
Science
Volume: 355
Issue: 6323
Pages: 398-403
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Cellular tagging as a neural network mechanism for behavioral tagging.2016
Author(s)
Nomoto, M., Ohkawa, N., Nishizono, H., Yokose, J., Suzuki, A., Matuo, M., Tsujimura, S., Takahashi, Y., Nagase, M., Watabe, A.M., Kato, F., Inokuchi, K.
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Journal Title
Nature Communications
Volume: 7:12319
Issue: 1
Pages: 1-11
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Integrating Neuronal Ensembles Constitute and Replay Hippocampal Engram2018
Author(s)
Ghandour K., Ohkawa N., Fung C.C.A., Asai H., Saitoh Y., Takekawa T., Okubo-Suzuki R., Soya S., Nishizono H., Matsuo M., Sato M., Ohkura M., Nakai J., Hayashi Y., Sakurai T., Osanai M., Kitamura T.,Fukai T, and Inokuchi K.
Organizer
The 41st Annual Meeting of the Japan Neuroscience Society. 2018. 7. 27, Kobe, Japan.
Related Report
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[Presentation] Establishment of in vivo field excitatory post synaptic potential (fEPSP) recording system at hippocampal CA3-CA3 synapses2018
Author(s)
Oishi N., Koga K., Nomoto M., Ohkawa N., Tsujimura S., Sano Y., Saitoh Y., Nishizono H., Matsuo M., Muramatsu S, and Inokuchi K.
Organizer
The 41st Annual Meeting of the Japan Neuroscience Society. 2018. 7. 27, Kobe, Japan.
Related Report
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[Presentation] Impairment of memory consolidation by anesthesia immediately after conditioning2018
Author(s)
Kameyama A., Asai H., Saitoh Y., Ghandour K., Okubo-Suzuki R., Nomoto M., Tsujimura S., Ohkawa N, and Inokuchi K.
Organizer
The 41st Annual Meeting of the Japan Neuroscience Society. 2018. 7. 27, Kobe, Japan.
Related Report
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[Presentation] Orchestrated ensembles activity constitute hippocampal memory engram2018
Author(s)
Ghandour K., Ohkawa N., Fung C. A., Asai H, Saitoh Y., Takekawa T., Okubo-Suzuki R., Soya S., Nishizono H., Matsuo M. , Osanai M., Sato M., Ohkura M., Nakai J., Hayashi Y., Sakurai T., Kitamura T., Fukai T., and Inokuchi K.
Organizer
The 17th Annual MCCS meeting, 2018. 11. 1, San Diego, CA, USA.
Related Report
Int'l Joint Research
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[Presentation] Manipulation of fear memory association by posterior parietal cortex2018
Author(s)
Suzuki A., Kosugi S., Murayama E., Ohkawa N., Matsuo M., Nishizono H., and Inokuchi K.
Organizer
The 17th Annual MCCS meeting, 2018. 11. 1, San Diego, CA, USA.
Related Report
Int'l Joint Research
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[Presentation] Orchestrated ensembles activity constitute hippocampal memory engram2018
Author(s)
Ghandour K., Ohkawa N., Fung C. A., Asai H, Saitoh Y., Takekawa T., Okubo-Suzuki R., Soya S., Nishizono H., Matsuo M. , Osanai M., Sato M., Ohkura M., Nakai J., Hayashi Y., Sakurai T., Kitamura T., Fukai T., and Inokuchi K.
Organizer
Neuroscience 2018, Annual Meeting of Society for Neuroscience, 2018. 11. 4, San Diego, CA, USA.
Related Report
Int'l Joint Research
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[Presentation] Manipulation of fear memory association by posterior parietal cortex2018
Author(s)
Suzuki A., Kosugi S., Murayama E., Ohkawa N., Matsuo M., Nishizono H., and Inokuchi K.
Organizer
Neuroscience 2018, Annual Meeting of Society for Neuroscience, 2018. 11. 4, San Diego, CA, USA.
Related Report
Int'l Joint Research
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[Presentation] Identification of characteristic dynamism of engram cells during learning2017
Author(s)
Ghandour K., Ohkawa N., Fung C.C.A., Saitoh Y., Takekawa T., Asai H., Okubo-Suzuki R., Nomoto M., Tsujimura S., Nishizono H., Matsuo M., Tsujimura M., Ohkura M., Nakai J., Hayashi Y., Fukai T., and Inokuchi K.
Organizer
第40回日本神経科学大会
Related Report
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[Presentation] Ultra-thin fluorescence endoscope imaging system for functional brain imaging2017
Author(s)
Osanai M., Ohkawa N., Sakamoto K., Miwa H., Kikuta S., Tamura A., Sato A., Ohkura M., Kojima T., Kohmura Y., Nakai J., Hayashi Y., Yanagawa Y., Inokuchi K., Homma N., Mushiake H.
Organizer
第40回日本神経科学大会
Related Report
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[Presentation] Unraveling the dynamism of engram cells during contextual memory processing2017
Author(s)
Ghandour K., Ohkawa N., Fung C.C.A., Saitoh Y., Takekawa T., Asai H., Okubo-Suzuki R., Nomoto M., Soya S., Tsujimura S., Nishizono H., Matsuo M., Sato M., Ohkura M., Nakai J., Hayashi Y., Sakurai T., Osanai M., Fukai T., and Inokuchi K.
Organizer
The 16th Annual MCCS meeting
Related Report
Int'l Joint Research
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[Presentation] Unraveling the dynamism of engram cells during contextual memory processing2017
Author(s)
Ghandour K., Ohkawa N., Fung C.C.A., Saitoh Y., Takekawa T., Asai H., Okubo-Suzuki R., Nomoto M., Soya S., Tsujimura S., Nishizono H., Matsuo M., Sato M., Ohkura M., Nakai J., Hayashi Y., Sakurai T., Osanai M., Fukai T., and Inokuchi K.
Organizer
Neuroscience 2017, Annual Meeting of Society for Neuroscience
Related Report
Int'l Joint Research
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[Presentation] Neuronal ensemble orchestrated intersection of two distinct emotional memory traces2016
Author(s)
Yokose J., Okubo-Suzuki R., Nomoto M., Ohkawa N., Nishizono H., Matsuo M., Suzuki A., Takahashi Y., Nagase M., Watabe A.M., Sasahara M., Kato F., and Inokuchi K.
Organizer
Neuroscience 2016, Annual Meeting of Society for Neuroscience
Place of Presentation
San Diego, USA
Year and Date
2016-11-12
Related Report
Int'l Joint Research
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[Presentation] The functional role of neuronal ensemble activated in intersection of two distinct memories2016
Author(s)
Yokose J., Nomoto M., Okubo-Suzuki R., Takahashi Y., Suzuki A., Ohkawa N., Nishizono H., Matsuo M., Watabe A.M., Kato F., Inokuchi K.
Organizer
第39回日本神経科学大会
Place of Presentation
横浜
Year and Date
2016-07-20
Related Report
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