Development of sensitive analytical method for protein and nucleic acid using natural elemental tags
Project/Area Number |
21550094
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Analytical chemistry
|
Research Institution | National Institute of Advanced Industrial Science and Technology |
Principal Investigator |
TAKATSU Akiko 独立行政法人産業技術総合研究所, 計測標準研究部門, 研究科長 (10357361)
|
Co-Investigator(Kenkyū-buntansha) |
INAGAKI Kazumi 独立行政法人産業技術総合研究所, 計測標準研究部門, 研究室付 (50356490)
FUJII Shin-ichiro 独立行政法人産業技術総合研究所, 計測標準研究部門, 研究員 (10415739)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 生体分析 / 核酸 / タンパク質 / 誘導結合プラズマ質量分析 / 液体クロマトグラフィー / キャピラリー電気泳動 / リン定量 |
Research Abstract |
A high performance interface device was developed for sample introduction to inductively coupled plasma mass spectrometer(ICP-MS) at low flow rate. Using this interface device, hyphenation of capillary liquid chromatography(LC) or capillary electrophoresis with ICP-MS was achieved. DNA molecules separated with CE or capillary LC were sensitively and selectively detected with ICP-MS in quantification of phosphorus as an elemental tag. We have concluded that this system can provide a fast screening method for various bio-molecules in biological samples.
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Report
(4 results)
Research Products
(13 results)