Study on physicochemical process of radiation damage for DNA adsorbed on self-assembled monolayer
Project/Area Number |
25740020
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Risk sciences of radiation and chemicals
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Research Institution | National Institute of Advanced Industrial Science and Technology (2014-2015) Japan Atomic Energy Agency (2013) |
Principal Investigator |
Narita Ayumi 国立研究開発法人産業技術総合研究所, 省エネルギー研究部門, 産総研特別研究員 (50633898)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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Keywords | DNA / 自己組織化単分子膜 / 放射光軟X線 / X線光電子分光 / 吸収端近傍X線吸収微細構造 / 放射線影響 / 吸収端近傍X線吸収微細構造法 / DNA損傷過程 / 生体分子固定化 / 酸化物表面 / 軟X線 / X線吸収微細構造法 / X線光電子分光法 / 放射光 |
Outline of Final Research Achievements |
A novel two-layer sample composed of a deoxyribonucleic acid (DNA) film and self-assembledmonolayer (SAM) was prepared on an inorganic surface. A SAM was formed on a sapphire surface, then oligonucleotide (OGN) molecules were adsorbed on the MPTS-SAM. The thicknesses and chemical states of the layers were determined. The sample was irradiated with synchrotron soft X-rays. The chemical state of the OGN molecules before and after irradiation was examined by Near-edge X-ray fine structure (NEXAFS). The peak shape of the N K-edge NEXAFS spectra of the MPTS-OGN layers clearly changed following irradiation.The MPTS-OGN layer formed on the sapphire surface. The chemical states and the structure of the interface were elucidated using synchrotron soft X-rays. The OGN molecules adsorbed on the MPTS films decomposed upon exposure to soft X-ray irradiation.
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Report
(4 results)
Research Products
(9 results)