Development of new imaging methods to analyze the mechanism of cell differentiation in trees by direct visualization of single-cell
Project/Area Number |
16K14954
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Wood science
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Research Institution | Tokyo University of Agriculture and Technology |
Principal Investigator |
Funada Ryo 東京農工大学, (連合)農学研究科(研究院), 教授 (20192734)
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | 木質バイオマス / 細胞分化 / 生体イメージング / 細胞壁 / 微小管 / 管状要素 / 培養細胞 / セルロース・ミクロフィブリル / 細胞壁構造 / 細胞骨格 / 植物ホルモン / 共焦点レーザ走査顕微鏡法 / アクチンフィラメント / 共焦点レーザ顕微鏡 / 生体イメージング解析 |
Outline of Final Research Achievements |
In order to understand fully the mechanism of xylem differentiation in trees, new differentiation system that could induce formation of secondary xylem-like tracheary elements was developed from from calli of Populus sieboldi × P. grandidentata and Aesculus turbinate. Induced tracheary elements formed broad regions of thick cell walls, bordered pits and perforations that showed typical features of vessel elements and tracheids. Our new in vitro system allows the detailed analysis of the process of differentiation of secondary xylem. In addition, the orientation and localization of microtubules was visualized using stable expression of genes for microtubule-associated proteins with green fluorescent protein in a single differentiating tracheary element by real-time imaging techniques. The microtubules play an important role in the determination of pattern of cell wall structure.
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Report
(3 results)
Research Products
(30 results)
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[Journal Article] Climate change and the regulation of wood formation in trees by temperature2018
Author(s)
Begum, S., Kudo, K., Rahman, Md H., Nakaba, S., Yamagishi, Y., Nabeshima, E., Nugroho, D.W., Oribe, Y., Kitin, P., Jin H.O., Funada, R.
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Journal Title
Trees-Structure and Function
Volume: 32
Issue: 1
Pages: 3-15
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Stem gravitropism and tension wood formation in Acacia mangium seedlings inclined at various angles2018
Author(s)
Nugroho, W.D., Nakaba, S., Yamagishi, Y., Begum, S., Rahman, M.H., Kudo, K., Marsoem, S.N., Funada, R.
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Journal Title
Annals of Botany
Volume: 印刷中
Issue: 1
Pages: 87-94
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Responses of ray parenchyma cells to wounding differ between earlywood and latewood in the sapwood of Cryptomeria japonica2017
Author(s)
Nakaba, S., Morimoto, H., Arakawa, I., Yamagishi, Y., Nakada, R., Funada, R.
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Journal Title
Trees
Volume: 31
Issue: 1
Pages: 31-39
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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