<i>KISS1</i> Gene Expression in the Developing Brain of Female Pigs in Pre- and Peripubertal Periods
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- IEDA Nahoko
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- UENOYAMA Yoshihisa
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- TAJIMA Yoko
- Aichi Agricultural Research Center, Nagakute 480-1193, Japan
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- NAKATA Tomoko
- Aichi Agricultural Research Center, Nagakute 480-1193, Japan
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- KANO Masatoshi
- Aichi Agricultural Research Center, Nagakute 480-1193, Japan
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- NANIWA Yousuke
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- WATANABE Youki
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- MINABE Shiori
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- TOMIKAWA Junko
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- INOUE Naoko
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- MATSUDA Fuko
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- OHKURA Satoshi
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
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- MAEDA Kei-ichiro
- Department of Veterinary Medical Sciences, The University of Tokyo, Tokyo 113-8657, Japan
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- TSUKAMURA Hiroko
- Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
Bibliographic Information
- Other Title
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- KISS1 Gene Expression in the Developing Brain of Female Pigs in Pre- and Peripubertal Periods
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Abstract
Puberty is associated with an increase in gonadotropin secretion as a result of an increase in gonadotropin-releasing hormone (GnRH) secretion. Kisspeptin is considered to play a key role in puberty onset in many mammalian species, including rodents, ruminants and primates. The present study aimed to determine if changes in hypothalamic expression of the KISS1 gene, encoding kisspeptin, are associated with the onset of puberty in pigs. The animals (n=4 in each group) were perfused with 4% paraformaldehyde at 0, 1, 2, 3 and 4 months old, as prepubertal stages, and at 5 months old, as the peripubertal stage, following each blood sampling. KISS1 gene expressions in coronal sections of brains were visualized by in situ hybridization. Plasma luteinizing hormone (LH) was measured by radioimmunoassay. KISS1 mRNA signals were observed in the arcuate nucleus (ARC) at all ages examined without any significant difference in the number of KISS1-expressing cells, indicating that the KISS1 gene is constantly expressed in the ARC throughout pubertal development in pigs. The plasma LH concentration was the highest in 0-month-old piglets and significantly decreased in the 1- and 2 month-old groups (P<0.05), suggesting a developing negative feedback mechanism affecting gonadotropin release during the prepubertal period. Considering the potent stimulating effect of kisspeptin on gonadotropin release in prepubertal pigs, kisspeptin secretion rather than kisspeptin synthesis may be responsible for the onset of puberty in pigs.
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 60 (4), 312-316, 2014
The Society for Reproduction and Development
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Details
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- CRID
- 1390001206337689728
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- NII Article ID
- 130004137827
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- NII Book ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC2cfgtlWquw%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 025629542
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- PubMed
- 24909600
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed