Establishment of conditional knockout animal model for analyzing the pathogenesis of follicular cyst in the goat.
Project/Area Number |
23658223
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Applied animal science
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Research Institution | Nagoya University |
Principal Investigator |
OHKURA Satoshi 名古屋大学, 生命農学研究科, 教授 (20263163)
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Co-Investigator(Renkei-kenkyūsha) |
FURUSAWA Tadashi 独立行政法人農業生物資源研究所, 動物科学研究領域, 研究員 (00343997)
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Research Collaborator |
MATSUDA Fuko 名古屋大学, 大学院・生命農学研究科, 准教授 (10608855)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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Keywords | 応用動物 / 神経科学 / 獣医学 / 生理学 / 畜産学 / ヤギ / キスペプチン / 卵胞嚢腫 |
Research Abstract |
In the present study, as the initial step in establishing a region-specific, conditional KISS1 gene knockout goat, we first revealed the genomic sequence of goat KISS1 gene 5'-upstream region up to 3 kb from translation initiation site. This information can be used for further evaluation of the promoter activity of goat KISS1 gene expression and establishment of conditional knockout model. Second, we demonstrated that an intravenous infusion of estrogen induced surge-like luteinizing hormone (LH) release both in gonadectomized male and female goats, and kisspeptin neurons in the medial preoptic area (mPOA) were activated by estrogen. In castrated male goats, mean peak LH concentration was lower and mean peak time of the LH surge was later than females. These results suggest that deterioration in functioning of mPOA kisspeptin neurons in response to estrogen in male goats can be applied as a model for analyzing the neuronal pathogenesis of follicular cyst in female ruminants.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Molecular Cloning and Identification of the Transcriptional Regulatory Domain of the Goat Neurokinin B Gene <i>TAC3</i>2013
Author(s)
Suetomi, Y., Matsuda, F., Uenoyama, Y., Maeda, K.-I., Tsukamura, H. and Ohkura, S.
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Journal Title
Journal of Reproduction and Development
Volume: 59
Issue: 5
Pages: 463-469
DOI
NAID
ISSN
0916-8818, 1348-4400
Related Report
Peer Reviewed
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[Journal Article] Effects of Full-Length Kisspeptin Administration on Follicular Development in Japanese Black Beef Cows2013
Author(s)
Naniwa, Y., Nakatsukasa, K., Setsuda, S., Oishi, S., Fujii, N., Matsuda, F., Uenoyama, Y., Tsukamura, H., Maeda, K. -I. and Ohkura, S.
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Journal Title
Journal of Reproduction and Development
Volume: 59
Issue: 6
Pages: 588-594
DOI
NAID
ISSN
0916-8818, 1348-4400
Related Report
Peer Reviewed
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[Journal Article] Differential Effects of Continuous Exposure to the Investigational Metastin/Kisspeptin Analog TAK-683 on Pulsatile and Surge Mode Secretion of Luteinizing Hormone in Ovariectomized Goats2013
Author(s)
Tanaka, T., Ohkura, S., Wakabayashi, Y., Kuroiwa, T., Nagai, K., Endo, N., Tanaka, A., Matsui, H., Kusaka, M. and Okamura, H.
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Journal Title
Journal of Reproduction and Development
Volume: 59
Issue: 6
Pages: 563-568
DOI
NAID
ISSN
0916-8818, 1348-4400
Related Report
Peer Reviewed
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[Journal Article] Central distribution of kiss2 neurons and peri-pubertal changes in their expression in the brain of the male and female red seabream Pagrus major2011
Author(s)
Shimizu, Y., Tomikawa, J., Hirano, K., Nanikawa, Y., Akazome, Y., Kanda, S., Kazeto, Y., Okuzawa, K., Uenoyama, Y., Ohkura, S., Tsukamura, H., Maeda, K.-I., Gen, K., Oka, Y., and Yamamoto, N.
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Journal Title
General and Comparative Endocrinology
Volume: 175
Issue: 3
Pages: 432-442
DOI
Related Report
Peer Reviewed
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[Presentation] Estrogen induces surge-like luteinizing hormone secretion in gonadectomized male and female goats: involvement of kisspeptin neurons in the medial preoptic area in both sexes.2013
Author(s)
Ohkura, S., Nakatsukasa, K., Suetomi Y., Naniwa, Y., Ito, D., Inoue, N., Wakabayashi, Y., Okamura, H., Maeda, K.-I., Uenoyama, Y., Tsukamura, H. and Matsuda, F.
Organizer
The 46th Annual Meeting of the Society for the Study of Reproduction
Place of Presentation
Montreal, Canada
Related Report
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[Presentation] Effects of metastin/kisspeptin analog, TAK-683, on luteinizing hormone secretion in peripheral plasma, and gonadotropin-releasing hormone secretion in the pituitary portal circulation in goats.2011
Author(s)
Ohkura, S., Tanaka, T., Kuroiwa, T. Wakabayashi, Y., Ohtaki, T., Kusaka, M. and Okamura, H.
Organizer
The Endocrine Society 93rd Annual Meeting & Expo (ENDO 2011)
Place of Presentation
Boston, USA、Boston Convention & Exhibition Center
Related Report
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[Presentation] Effects of chronic administration of a metastin analog, TAK-683, on the GnRH pulse generator activity in ovariectomized goats.2011
Author(s)
Wakabayashi, Y., Yamamura, T., Ohkura, S., Tanaka, T., Kusaka, M. and Okamura, H.
Organizer
The Endocrine Society 93rd Annual Meeting & Expo (ENDO 2011)
Place of Presentation
Boston, USA、Boston Convention & Exhibition Center
Related Report
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[Presentation] Male kisspeptin neuronal network and its interaction with GnRH neurons in goats.2011
Author(s)
Ohkura, S., Matsuyama, S., Mogi, K., Wakabayashi, Y., Mori, Y., Tsukamura, H., Maeda, K.-I., Ichikawa, M. and Okamura, H.
Organizer
The 2nd World Congress on Reproductive Biology
Place of Presentation
Cairns, Australia、Cairns Convention Centre
Related Report
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[Presentation] Differential effects of continuous exposure to the investigational metastin/kisspeptin analog TAK-683 on pulsatile and surge mode secretion of luteinizing hormone in ovariectomized goats.2011
Author(s)
Tanaka, T., Ohkura, S., Wakabayashi, Y., Tanaka, A., Kusaka, M. and Okamura, H.
Organizer
The 2nd World Congress on Reproductive Biology
Place of Presentation
Cairns, Australia、Cairns Convention Centre
Related Report
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[Presentation] Kisspeptin neurons in the medial preoptic area of castrated male goats mediate the surge-like luteinizing hormone secretion induced by acute elevations in circulating estrogen.
Author(s)
Nakatsukasa, K., Suetomi, Y., Naniwa, Y., Ito, D., Inoue, N., Wakabayashi, Y., Okamura, H., Maeda, K.-I., Uenoyama, Y., Tsukamura, H., Matsuda, F. and Ohkura, S.
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
Tokyo, Japan
Related Report
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[Presentation] Involvement of kisspeptin neurons in the regulation of estrogen-induced luteinizing hormone surge in both sexes of the Japanese macaque (Macaca fuscata fuscata).
Author(s)
Watanabe, Y., Uenoyama, Y., Ieda, N., Kawahara, M., Takase, K., Suetomi, Y., Suzuki, J., Ohkura, S., Maeda, K.-I. and Tsukamura, H.
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
Tokyo, Japan
Related Report
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[Presentation] Estrogen induces surge-like luteinizing hormone secretion in gonadectomized male and female goats: involvement of kisspeptin neurons in the medial preoptic area in both sexes.
Author(s)
Ohkura, S., Nakatsukasa, K., Suetomi, Y., Naniwa, Y., Ito, D., Inoue, N., Wakabayashi, Y., Okamura, H., Maeda, K.-I., Uenoyama, Y., Tsukamura, H. and Matsuda, F.
Organizer
The 46th Annual Meeting of the Society for the Study of Reproduction
Place of Presentation
Montreal, Canada
Related Report
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[Book] Chapter 14: Kisspeptin and GnRH pulse generation. In Kisspeptin Signaling in Reproductive Biology(Eds. A.S. Kauffman and J.T.Smith.)2013
Author(s)
Okamura, H., Tsukamura, H., Ohkura, S., Uenoyama, Y., Wakabayashi, Y. and Maeda, K.-I
Publisher
Springer, Berlin
Related Report
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[Book] Kisspeptin Signaling in Reproductive Biology2013
Author(s)
Okamura, H., Tsukamura, H., Ohkura, S., Uenoyama, Y., Wakabayashi, Y. and Maeda, K.-I.
Total Pages
514
Publisher
Springer
Related Report
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