Study on a regulatory mechanism of follicular growth in goats by applying optogenetic technology
Project/Area Number |
24380154
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Applied animal science
|
Research Institution | Nagoya University |
Principal Investigator |
Ohkura Satoshi 名古屋大学, 生命農学研究科, 教授 (20263163)
|
Co-Investigator(Kenkyū-buntansha) |
MATSUDA Fuko 東京大学, 大学院農学生命科学研究科, 准教授 (10608855)
|
Co-Investigator(Renkei-kenkyūsha) |
FURUSAWA Tadashi 農業・食品産業技術総合研究機構, 畜産研究部門, 主任研究員 (00343997)
OHKOSHI Katsuhiro 農業・食品産業技術総合研究機構, 畜産研究部門, 主任研究員 (00414889)
|
Research Collaborator |
KOBAYASHI Kenta
SUETOMI Yuta
TATEBAYASHI Ryoki
OKUDA Kazuhiro
KATO Masahiro
HASSANEEN Ahmed Saad Ahmed
|
Project Period (FY) |
2012-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
|
Keywords | 応用動物 / 神経科学 / 獣医学 / 畜産学 / GnRH / ヤギ / キスペプチン / ニューロキニンB |
Outline of Final Research Achievements |
The present study aimed to elucidate the hypothalamic mechanism controlling the follicular growth in ruminants. First, the genome structure of the goat neurokinin B gene (TAC3) and a transcriptional regulatory domain of TAC3 were identified. Second, to generate genetically modified goats which could control the activity of KNDy neurons (neurons contain neurokinin B) in the hypothalamic arcuate nucleus by using an optogenetic technology, we applied the gene introduction method by the adeno-associated virus vector and Cre-loxP system in goats to establish a KNDy neuron-specific expression of light-responsive ion channels. Third, we demonstrated the localization of KNDy neurons in the hypothalamic arcuate nucleus in cows by immunohistochemistry and suggested that KNDy neurons have a key role to stimulate gonadotropin-releasing hormone (GnRH) secretion, and then follicular growth in ruminants.
|
Report
(5 results)
Research Products
(63 results)
-
[Journal Article] The Luteinising Hormone Surge-Generating System is Functional in Male Goats as in Females: Involvement of Kisspetin Neurons in the Medial Preoptic Area2015
Author(s)
F. Matsuda, K. Nakatsukasa, Y. Suetomi, Y. Naniwa, D. Ito, N. Inoue, Y. Wakabayashi, H. Okamura, K.-I. Maeda, Y. Uenoyama, H. Tsukamura and S. Ohkura
-
Journal Title
Journal of Neuroendocrinology
Volume: 27
Issue: 1
Pages: 57-65
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
[Journal Article] Structure-activity relationship study on senktide for development of novel potent neurokinin-3 receptor selective agonists.2015
Author(s)
Misu R, Yamamoto K, Yamada A, Noguchi T, Ohno H, Yamamura T, Okamura H, Matsuda F, Ohkura S, Oishi S, Fujii N.
-
Journal Title
MedChemCommun
Volume: 6
Issue: 3
Pages: 469-476
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Differential changes in luteinizing hormone secretion after administration of the investigational metastin/kisspeptin analog TAK-683 in goats.2015
Author(s)
Endo, N., Tamesaki, C., Ohkura, S., Wakabayashi, Y., Matsui, H., Tanaka, A., Watanabe, T., Okamura, H. and Tanaka, T.
-
Journal Title
Animal Reproduction Science
Volume: 159
Pages: 87-93
DOI
Related Report
Peer Reviewed
-
[Journal Article] Ovarian and hormonal responses to follicular phase administration of investigational metastin/kisspeptin analog, TAK-683, in goats.2014
Author(s)
Goto, Y., Endo, N., Nagai, K., Ohkura, S., Wakabayashi, Y., Tanaka, A., Matsui, H., Kusaka, M., Okamura, H. and Tanaka, T.
-
Journal Title
Reproduction in Domestic Animals
Volume: 49
Issue: 2
Pages: 338-342
DOI
Related Report
Peer Reviewed
-
[Journal Article] <i>KISS1</i> Gene Expression in the Developing Brain of Female Pigs in Pre- and Peripubertal Periods2014
Author(s)
Ieda, N., Uenoyama, Y., Tajima, Y., Nakata, T., Kano, M., Naniwa, Y., Watanabe, Y., Minabe, S., Tomikawa, J., Inoue, N., Matsuda, F., Ohkura, S., Maeda, K.-I. and Tsukamura, H.
-
Journal Title
Journal of Reproduction and Development
Volume: 60
Issue: 4
Pages: 312-316
DOI
NAID
ISSN
0916-8818, 1348-4400
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Development of novel neurokinin 3 receptor (NK3R) selective agonists with resistance to proteolytic degradation.2014
Author(s)
Misu R, Oishi S, Yamada A, Yamamura T, Matsuda F, Yamamoto K, Noguchi T, Ohno H, Okamura H, Ohkura S, Fujii N.
-
Journal Title
J. Med. Chem.
Volume: 57
Issue: 20
Pages: 8646-8651
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] The effects of chronic subcutaneous administration of an investigational kisspeptin analogue, TAK-683, on gonadotropin-releasing hormone pulse generator activity in goats.2014
Author(s)
Yamamura, T., Wakabayashi, Y., Sakamoto, K., Matsui, H., Kusaka, M., Tanaka, T., Ohkura, S. and Okamura, H.
-
Journal Title
Neuroendocrinology
Volume: 100
Issue: 2-3
Pages: 250-264
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Molecular Cloning and Identification of the Transcriptional Regulatory Domain of the Goat Neurokinin B Gene <i>TAC3</i>2013
Author(s)
Suetomi, Y., Matsuda, F., Uenoyama, Y., Maeda, K.-I., Tsukamura, H. and Ohkura, S.
-
Journal Title
Journal of Reproduction and Development
Volume: 59
Issue: 5
Pages: 463-469
DOI
NAID
ISSN
0916-8818, 1348-4400
Related Report
Peer Reviewed
-
[Journal Article] Effects of Full-Length Kisspeptin Administration on Follicular Development in Japanese Black Beef Cows2013
Author(s)
Naniwa, Y., Nakatsukasa, K., Setsuda, S., Oishi, S., Fujii, N., Matsuda, F., Uenoyama, Y., Tsukamura, H., Maeda, K. -I. and Ohkura, S.
-
Journal Title
Journal of Reproduction and Development
Volume: 59
Issue: 6
Pages: 588-594
DOI
NAID
ISSN
0916-8818, 1348-4400
Related Report
Peer Reviewed
-
[Journal Article] Differential Effects of Continuous Exposure to the Investigational Metastin/Kisspeptin Analog TAK-683 on Pulsatile and Surge Mode Secretion of Luteinizing Hormone in Ovariectomized Goats2013
Author(s)
Tanaka, T., Ohkura, S., Wakabayashi, Y., Kuroiwa, T., Nagai, K., Endo, N., Tanaka, A., Matsui, H., Kusaka, M. and Okamura, H.
-
Journal Title
Journal of Reproduction and Development
Volume: 59
Issue: 6
Pages: 563-568
DOI
NAID
ISSN
0916-8818, 1348-4400
Related Report
Peer Reviewed
-
-
-
[Journal Article] Central distribution of kiss2 neurons and peri-pubertal changes in their expression in the brain of male and female red seabream Pagrus major.2012
Author(s)
Shimizu, Y, Tomikawa, J., Hirano, K., Nanikawa, Y., Akazome, Y., Kanda, S., Kazeto, Y., Okuzawa, K., Uenoyama, Y., Ohkura, S., Tsukamura, H., Maeda, K.-I., Gen, K, Oka, Y. and Yamamoto, N.
-
Journal Title
General and Comparative Endocrinology
Volume: 175
Pages: 432-442
Related Report
Peer Reviewed
-
-
-
-
-
[Presentation] Molecular cloning and identification of the transcriptional regulatory domain of the goat neurokinin B gene TAC3.2015
Author(s)
Suetomi, Y., Matsuda, F., Uenoyama, Y., Tsukamura, H., Maeda, K.-I. and Ohkura, S.
Organizer
International Conference on Biology and Pathology of Reproduction in Domestic Animals
Place of Presentation
Gdansk, Poland
Year and Date
2015-09-28
Related Report
Int'l Joint Research
-
-
-
-
-
-
[Presentation] Development of novel neurokinin-3 receptor selective agonists with registance against proteolytic degradation.2014
Author(s)
Misu, R., Oishi, S., Yamada, A., Yamamoto, K., Noguchi, T., Ohno, H., Yamamura, T., Okamura, H., Matsuda, F., Ohkura, S. and Fujii, N.
Organizer
The 51st Japanese Peptide Symposium
Place of Presentation
Tokushima, Japan
Year and Date
2014-10-22 – 2014-10-24
Related Report
-
-
[Presentation] Development of novel neurokinin-3 receptor agonists that potently stimulate the GnRH pulse generator.2014
Author(s)
Yamamura, T., Misu, R., Wakabayashi, Y., Ohkura, S., Oishi, S., Fujii, N. and Okamura, H.
Organizer
The 3rd World Congress on Reproductive Biology
Place of Presentation
Edinburgh, UK
Year and Date
2014-09-02 – 2014-09-04
Related Report
-
[Presentation] Differential changes in LH secretion after administration of investigational metastin/kisspeptin analog TAK-683 to goats.2014
Author(s)
Tamesaki, C., Endo, N., Ohkura, S., Wakabayashi, Y., Matsui, H., Tanaka, A., Watanabe, T., Okamura, H. and Tanaka, T.
Organizer
The 3rd World Congress on Reproductive Biology
Place of Presentation
Edinburgh, UK
Year and Date
2014-09-02 – 2014-09-04
Related Report
-
[Presentation] Endocrine responses and ovarian dynamics in goats treated with low dose of investigational metastin/kisspeptin analog TAK-683 in follicular phase.2014
Author(s)
Gomi, N., Endo, N., Ohkura, S., Wakabayashi, Y., Matsui, H., Matsumoto, H., Ishikawa, K., Tanaka, A., Watanabe, T., Okamura, H. and Tanaka, T.
Organizer
The 3rd World Congress on Reproductive Biology
Place of Presentation
Edinburgh, UK
Year and Date
2014-09-02 – 2014-09-04
Related Report
-
[Presentation] Establishment of immortalized neuronal cell lines derived from domestic ruminant hypothalamus.2014
Author(s)
Suetomi, Y., Kato, M., Matsuyama, S., Kimura, K., Tsukamura, H., Ohkura, S. and Matsuda, F.
Organizer
9th International Ruminant Reproduction Symposium
Place of Presentation
Obihiro, Japan
Year and Date
2014-08-25 – 2014-08-29
Related Report
-
-
-
-
-
-
-
[Presentation] Establishment of immortalized neuronal cell lines derived from domestic ruminant hypothalamus.2014
Author(s)
Suetomi, Y., Kato, M., Matsuyama, S., Kimura, K., Tsukamura, H., Ohkura, S. and Matsuda, F.
Organizer
The 9th International Ruminant Reproduction Symposium
Place of Presentation
Obihiro, Japan
Related Report
-
[Presentation] Cloning of full-length mRNA and 5’-upstream DNA sequences of goat TAC3 and the analysis of its transcriptional regulatory mechanisms.2013
Author(s)
Suetomi, Y., Matsuda, F., Uenoyama, Y., Tsukamura, H., Maeda, K.-I. and Ohkura, S.
Organizer
The 46th Annual Meeting of the Society for the Study of Reproduction
Place of Presentation
Montreal, Canada
Related Report
-
[Presentation] Generation of immortalized neuronal cell lines derived from bovine hypothalamic arcuate nucleus and medial preoptic area.2013
Author(s)
Matsuda, F., Suetomi, Y., Hassaneen, A.S.A., Kato, M., Matsuyama, S., Kimura, K. and Ohkura, S.
Organizer
The 46th Annual Meeting of the Society for the Study of Reproduction
Place of Presentation
Montreal, Canada
Related Report
-
[Presentation] Kisspeptin analog, TAK-683, has a potent bioactivity to stimulate follicular development and luteinizing hormone secretion in Japanese Black beef cows.2013
Author(s)
Naniwa, Y., Nakatsukasa, K., Suetomi, Y., Matsuda, F., Matsui, H., Kusaka, M., Ohtaki, T., Tanaka, T., Okamura, H. and Ohkura, S.
Organizer
The 46th Annual Meeting of the Society for the Study of Reproduction
Place of Presentation
Montreal, Canada
Related Report
-
[Presentation] Structure-activity relationship study of tachykinin peptides for development of novel neurokinin-3 receptor selective agonists.2013
Author(s)
Misu, R., Oishi, S., Noguchi, T., Yamada, A., Ohno, H., Matsuda, F., Ohkura, S. and Fujii, N.
Organizer
The 23rd American Peptide Symposium
Place of Presentation
Waikoloa, USA
Related Report
-
-
-
-
[Presentation] Evaluation of biological stability of neurokinin-3 receptor agonists for the development of novel biostable analogs.2013
Author(s)
Misu, R., Oishi, S., Yamada, A., Noguchi, T., Ohno, H., Matsuda, F., Ohkura, S. and Fujii, N.
Organizer
The 50th Japanese Peptide Symposium
Place of Presentation
Suita, Japan
Related Report
-
[Presentation] Generation of immortalized neuronal cell lines derived from goat hypothalamic arcuate nucleus and medial preoptic area.2012
Author(s)
Matsuda, F., Suetomi, Y., Uenoyama, Y., Maeda, K.-I., Tsukamura, H. and Ohkura, S.
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
Tokyo, Japan
Year and Date
2012-11-08
Related Report
-
[Presentation] Structure-activity relationship study of tachykinin peptides for development of novel neurokinin-3 receptor agonists.2012
Author(s)
Misu, R., Oishi, S., Noguchi, T., Yamada, A., Ohno, H., Yamamura, T., Okamura, H., Matsuda, F., Ohkura, S. and Fujii, N.
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
Tokyo, Japan
Year and Date
2012-11-08
Related Report
-
[Presentation] Cloning of goat TAC3 upstream region and evaluation of its promoter activity.2012
Author(s)
Suetomi, Y., Matsuda, F., Uenoyama, Y., Tsukamura, H., Maeda, K.-I. and Ohkura, S.
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
Tokyo, Japan
Year and Date
2012-11-07
Related Report
-
[Presentation] Continuous administration of senktide, a neurokinin B receptor agonist, facilitates intermittent activities of the GnRH pulse generator for 24 h in goats.2012
Author(s)
Yamamura, T., Wakabayashi, Y., Fujii, N., Oishi, S., Ohkura, S. and Okamura, H.
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
Tokyo, Japan
Year and Date
2012-11-07
Related Report
-
-
-
[Presentation] Analysis of the transcriptional regulatory mechanism of neurokinin B gene, TAC3, in the goat.2012
Author(s)
Matsuda, F., Suetomi, Y., Maeda, K.-I., Tsukamura, H. and Ohkura, S.
Organizer
Society for Neuroscience 42nd Annual Meeting
Place of Presentation
New Orleans, U.S.A.
Year and Date
2012-10-13
Related Report
-
[Presentation] Senktide, a neurokinin B receptor agonist, stimulates pulsatile LH secretion through a mechanism mediated by the GnRH pulse generator in goats.2012
Author(s)
Wakabayashi, Y., Yamamura, T., Ohkura, S., Homma, T., Sakamoto, K., Mori, Y. and Okamura, H.
Organizer
Society for Neuroscience 42nd Annual Meeting
Place of Presentation
New Orleans, U.S.A.
Year and Date
2012-10-13
Related Report
-
-
-
-
-
-
-
[Book] Kisspeptin Signaling in Reproductive Biology2013
Author(s)
Okamura, H., Tsukamura, H., Ohkura, S., Uenoyama, Y., Wakabayashi, Y. and Maeda, K.-I.
Total Pages
514
Publisher
Springer
Related Report
-