Antimicrobial Susceptibility and Methicillin Resistance in Staphylococcus pseudintermedius and Staphylococcus schleiferi subsp. coagulans Isolated from Dogs with Pyoderma in Japan
-
- KAWAKAMI Tetsuji
- The United Graduate School of Veterinary Sciences, Gifu University
-
- SHIBATA Sanae
- The United Graduate School of Veterinary Sciences, Gifu University
-
- MURAYAMA Nobuo
- ASC Dermatology Service
-
- NAGATA Masahiko
- ASC Dermatology Service
-
- NISHIFUJI Koji
- Department of Veterinary Medicine, Tokyo University of Agriculture and Technology
-
- IWASAKI Toshiroh
- Department of Veterinary Medicine, Tokyo University of Agriculture and Technology
-
- FUKATA Tsuneo
- The United Graduate School of Veterinary Sciences, Gifu University
Bibliographic Information
- Other Title
-
- Internal medicine: Antimicrobial susceptibility and methicillin resistance in Staphylococcus pseudintermedius and Staphylococcus schleiferi subsp. coagulans isolated from dogs with pyoderma in Japan
Search this article
Abstract
To understand species distribution, trends of antimicrobial susceptibility and prevalence of methicillin resistance in canine staphylococci in Japan, 190 coagulase-positive staphylococci (CoPS) were isolated from dogs with pyoderma in 2 Japanese veterinary referral hospitals. Using a multiplex polymerase chain reaction (M-PCR) method, two CoPS species were identified: 170 Staphylococcus pseudintermedius (89.5%) and 20 S. schleiferi subsp. coagulans isolates (10.5%). In these isolates, susceptibility to 7 antimicrobial agents was determined. Overall, the levels of susceptibility to cefalexin (CEX), amoxicillin/clavulanic acid (CVA/AMPC), minocycline (MINO), ofloxacin (OFLX), norfloxacin (NFLX), lincomycin (LCM) and clindamycin (CLDM) in S. pseudintermedius isolates were 38.2, 52.4, 34.7, 31.2, 34.1, 1.2 and 11.2%, respectively. In S. schleiferi subsp. coagulans isolates, 55% demonstrated susceptibility to CEX, 80% to CVA/AMPC, 70% to MINO, 45% to OFLX or NFLX and 30% to CLDM. None of S. schleiferi subsp. coagulans isolates was susceptible to LCM. To determine the prevalence of methicillin-resistant strains, we used a PCR method, which enabled detection of the fragment of mecA gene in 66.5% (113 of 170) in S. pseudintermedius and 30.0% (6 of 20) in S. schleiferi subsp. coagulans isolates. The frequencies of susceptibility to CEX, CVA/AMPC, OFLX, NFLX and CLDM were significantly lower in methicillin-resistant CoPS than in methicillin-susceptible CoPS isolates. These data suggest a high level of methicillin resistance in staphylococci isolated from dogs with pyoderma in Japan.<br>
Journal
-
- Journal of Veterinary Medical Science
-
Journal of Veterinary Medical Science 72 (12), 1615-1619, 2010
JAPANESE SOCIETY OF VETERINARY SCIENCE
- Tweet
Keywords
Details
-
- CRID
- 1390001206431116544
-
- NII Article ID
- 130000307697
- 80021550429
-
- NII Book ID
- AA10796138
-
- COI
- 1:CAS:528:DC%2BC3MXhtlOgu7s%3D
-
- ISSN
- 13477439
- 09167250
-
- NDL BIB ID
- 10954356
-
- PubMed
- 20703027
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- Abstract License Flag
- Disallowed