Elsevier

Mechanisms of Development

Volume 131, February 2014, Pages 127-136
Mechanisms of Development

A novel regulatory element for Shh expression in the lung and gut of mouse embryos

https://doi.org/10.1016/j.mod.2013.09.003Get rights and content
Under an Elsevier user license
open archive

Highlights

  • We found and identified a novel lung and gut enhancer for Shh.

  • The enhancer sequence was only seen in rodent species.

  • A patchwork of multiple enhancers regulate the seamless expression of Shh.

Abstract

Hedgehog (Hh) signaling plays pivotal roles in morphogenesis of several embryonic tissues, including the primitive gut. In the mouse embryo, Sonic hedgehog (Shh) is expressed in endodermal epithelia from the oral cavity to the intestine, and contributes to cell proliferation in the underlying mesenchyme and subsequent differentiation into the gastrointestinal smooth muscle. Three evolutionary conserved non-coding sequences in the region upstream of the Shh coding sequence contain endoderm-specific enhancers for Shh expression. Although Shh expression in the endodermal epithelial lining is mostly attributed to these three enhancers, none of them regulates gene expression in the gastroesophageal epithelium. Here, we found that a 1.7 Kb fragment located 100 Kb upstream of the Shh coding sequence contains a functional element for Shh expression in endodermal organs, including the esophagus and stomach. Compared with the three known endodermal enhancers, this novel enhancer shows less evolutionary conservation, even among rodents. In mouse embryonic endodermal tissues, the seamless expression of Shh is achieved by a patchwork of multiple enhancers with different rates of evolution.

Keywords

Sonic hedgehog
Enhancer
Gene regulation
Evolutionarily
Conservation
Mouse

Cited by (0)

1

Both authors contributed equally.