Mechanisms for development of diseases by H. pylori virulence factor complex
Project/Area Number |
16H05191
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Bacteriology (including mycology)
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Research Institution | Oita University |
Principal Investigator |
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2016: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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Keywords | ピロリ菌 / 外膜タンパク / タンパク質複合体 / 病原因子 / 炎症 / 抗体測定 / オルガノイド / ヘリコバクター・ピロリ / 感染症 / 癌 / 微生物 |
Outline of Final Research Achievements |
We succeeded to construct recombinant proteins of the whole length of OipA and CagA, the important virulence factors of Helicobacter pylori. Using mass spectrometry analyses, our results first suggested that OipA forms multimeric complex within the membrane region of H. pylori, or forms complex with other factors such as CagA. Furthermore, we established the most reliable system in Japan to measure anti-CagA antibody in serum samples. In addition, we succeeded to construct an ex-vivo model of human stomach, gastric epithelial organoid monolayers (gastric monolayers). Using this novel model, we expect to promote our research field on searching the interaction between H. pylori and gastric mucosa.
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Academic Significance and Societal Importance of the Research Achievements |
今回、我々が作成した血清中のCagA抗体を測定する系では、日本人由来のピロリ菌を材料としており、欧米のピロリ菌を用いている市販キットに比べて、正確性が高く、今後の汎用が期待される。また、今までは、ピロリ菌と胃粘膜との相互関係を調べるのに、胃がん細胞株が用いられていたが、2次元ガストロイドという新しい系を確立したことで、今後の基礎研究の飛躍的な発展が期待される。
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Report
(4 results)
Research Products
(163 results)
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[Journal Article] Helicobacter pylori bab characterization in clinical isolates from Bhutan, Myanmar, Nepal and Bangladesh.2017
Author(s)
Ansari S, Kabamba ET, Shrestha PK, Aftab H, Myint T, Tshering L, Sharma RP, Ni N, Aye TT, Subsomwong P, Uchida T, Ratanachu-Ek T, Vilaichone RK, Mahachai V, Matsumoto T, Akada J, Yamaoka Y.
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Journal Title
PLoS One.
Volume: 6
Issue: 11
Pages: 11-23
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Interleukin-17C in Human Helicobacter pylori Gastritis2017
Author(s)
Tanaka Shingo、Nagashima Hiroyuki、Cruz Modesto、Uchida Tomohisa、Uotani Takahiro、Jim?nez Abreu Jos? A.、Mahachai Varocha、Vilaichone Ratha-korn、Ratanachu-ek Thawee、Tshering Lotay、Graham David Y.、Yamaoka Yoshio
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Journal Title
Infection and Immunity
Volume: 85
Issue: 10
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Rapid evolution of distinct Helicobacter pylori subpopulations in the Americas.2017
Author(s)
Thorell K, Yahara K, Berthenet E, Lawson DJ, Mikhail J, Kato I, Mendez A, Rizzato C, Bravo MM, Suzuki R, Yamaoka Y, Torres J, Sheppard SK, Falush D.
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Journal Title
PLoS Genet
Volume: 13(2)
Issue: 2
Pages: 1-21
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Novel CagA ELISA exhibits enhanced sensitivity of Helicobacter pylori CagA antibody.2017
Author(s)
Matsuo Y, Kido Y, Akada J, Shiota S, Binh TT, Trang TT, Dung HD, Tung PH, Tri TD, Thuan NP, Tam LQ, Nam BC, Khien VV, Yamaoka Y.
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Journal Title
World J Gastroenterol
Volume: 23(1)
Issue: 1
Pages: 48-59
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Helicovanter pylori VacA, acting through receptor tyrosine phosphatase is crucial for CagA phosphorylation in human duodenal carcinoma cell line AZ-521.2016
Author(s)
Nakano, M., Yahiro, K., Yamazaki, E., Kurazono, H., Akada, J.K., Yamaoka, Y., Niidome, T., Hatakeyama, M., Suzuki, H., Yamamoto, T. Moss, J. and Hirayama, T.
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Journal Title
Dis. Model Mech.
Volume: 9
Issue: 12
Pages: 1473-1481
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Surveillance of Helicobacter pylori Antibiotic Susceptibility in Indonesia: Different Resistance Types among Regions and with Novel Genetic Mutations.2016
Author(s)
Miftahussurur M, Syam AF, Nusi IA, Makmun D, Waskito LA, Zein LH, Akil F, Uwan WB, Simanjuntak D, Wibawa ID, Waleleng JB, Saudale AM, Yusuf F, Mustika S, Adi P, Maimunah U, Maulahela H, Rezkitha YA, Subsomwong P, Nasronudin, Rahardjo D, Suzuki R, Akada J, Yamaoka Y.
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Journal Title
PLoS One
Volume: 11(12):e0166199
Issue: 12
Pages: 1-17
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] わが国における薬剤耐性Helicobacter pyloriの現状 - 2013~2014年度耐性菌サーベイランスの集計報告 -2016
Author(s)
橋永正彦, 沖本忠義, 兒玉雅明, 水上一弘, 小川竜, 岡本和久, 首藤充孝, 福田健介, 園田光, 福田昌英, 本田俊一郎, 山岡吉生, 村上和成
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Journal Title
日本ヘリコバクター学会誌
Volume: 17(2)
Pages: 20-24
Related Report
Peer Reviewed
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[Presentation] Validation of serum markers of precancerous condition in Mongolia, where burdens high gastric cancer2016
Author(s)
Boldbaatar G, Tegshee T, Khasag O, Duger D, Adiyasuren B, Uchida T, Subsomwong P, Matsuhisa T, Tsogt-Ochir B, Yamaoka Y.
Organizer
Asian Pacific Digestive Week (APDW) 2016
Place of Presentation
神戸国際会議場(兵庫県神戸市)
Year and Date
2016-11-02
Related Report
Int'l Joint Research
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[Presentation] A novel East Asian-type CagA antibody ELISA can detect H. pylori CagA antibody with higher sensitivity than commercial available CagA ELISA2016
Author(s)
Matsuo Y, Kido Y, Akada J, Shiota S, Binh TT, Trang TTH, Dung HDQ, Tung PH, Tri TD, Thuan NPM, Tam LQ, Nam BC, Khien VV, Yamaoka Y
Organizer
Asian Pacific Digestive Week (APDW) 2016
Place of Presentation
神戸国際会議場(兵庫県神戸市)
Year and Date
2016-11-02
Related Report
Int'l Joint Research
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[Presentation] Utility of gastro-endoscopy for subjects with serum H. pylori antibody (Hp-IgG) titer equal or more than 3.0 U/mL-Study of endoscopic findings for titers between 3.0 and 9.9 U/mL (so-called “Grayzone”).2016
Author(s)
Shuto M, Murakami K, Matsunari O, Okamoto K, Mizukami K, Okimoto T, Kodama M, Nonaka Y, Takigami S, Yamaoka Y, Fujioka T
Organizer
XXIXth International Workshop on Helicobacter and Microbiome in inflammation and Cancer.
Place of Presentation
マーゲブルク(ドイツ)
Year and Date
2016-09-15
Related Report
Int'l Joint Research
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[Presentation] Virulence factors and highly increasing antibiotics resistance of Helicobacter pylori in Vietnam2016
Author(s)
Dung HD, Binh TT, Tri TD, Tung NL, Long T, Phuong TNN, Murakami K, Fujioka T, Moriyama M, Uchida T, Yamaoka Y
Organizer
22nd annual meeting of the Japanese society for Helicobacter Research
Place of Presentation
別府ビーコンプラザ(大分県別府市)
Year and Date
2016-06-24
Related Report
Int'l Joint Research
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[Presentation] Human migration patterns in Asia revealed by H. pylori2016
Author(s)
Suzuki R, Matsumoto T, Akada J, Matsunari O, Shiota S, Binh TT, Trang HT, Miftahussurur M, Subsomwong P, Yamaoka Y
Organizer
22nd annual meeting of the Japanese society for Helicobacter Research
Place of Presentation
別府ビーコンプラザ(大分県別府市)
Year and Date
2016-06-24
Related Report
Int'l Joint Research
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