Development of a luminescence-based biosensor for detection of methylmercury
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- Nagata Takeshi
- Faculty of Pharmaceutical Sciences, Setsunan University
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- Muraoka Toshiaki
- Faculty of Pharmaceutical Sciences, Setsunan University
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- Kiyonoa Masako
- School of Pharmaceutical Sciences, Kitasato University
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- Pan-Hou Hidemitsu
- Faculty of Pharmaceutical Sciences, Setsunan University
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Abstract
A recombinant whole-cell bacterial sensor for highly selective and sensitive detection of the bioavailable methylmercury in the environment was constructed. The biosensor carries luciferase gene, luxAB, from Vibrio harveyi as a reporter under the control of the mercury inducible regulatory part of mer-operon from Pseudomonas K-62 plasmid pMR26. In addition, a merB gene encoding organomercurial lyase which cleaves the C-Hg bond of methylmercury to give Hg2+ was coexpressed in the sensor. The resulting bacterial sensor responded specifically to methylmercury, and the lowest detectable concentration of methylmercury was 10 pM with 1 ml sample in the optimized assay conditions. This detection limit is enough to detect this compound in many contaminated and some pristine environmental samples.
Journal
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- The Journal of Toxicological Sciences
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The Journal of Toxicological Sciences 35 (2), 231-234, 2010
The Japanese Society of Toxicology
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Details
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- CRID
- 1390001204901253248
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- NII Article ID
- 10026193447
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- NII Book ID
- AN00002808
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- ISSN
- 18803989
- 03881350
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- NDL BIB ID
- 10652019
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed