Genetic Engineering of Bacteria for Environmental Remediation of Mercury

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  • Remediation of hazardous waste contaminated soils

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Abstract

To prevent environmental mercury poisoning incidents, an effective technology for treating mercury-polluted environments is urgent. Recently, with advances in biotechnology, bioremediation utilizing microorganisms to remove mercurials from contaminated sites has become one of the most rapidly developing fields of environmental restoration. A number of bioremediation strategies, including biotransformation, biosorption, and bioprecipitation of mercurials, have been developed to treat mercurial-polluted environments and mercury-containing waste. To construct bacteria that are capable of specifically accumulating mercury, we have genetically engineered Escherichia coli to express a mercury transport system and organomercurial lyase enzyme simultaneously, and overexpress polyphosphate, a strong chelator of essential divalent metals. The mercury accumulation system was designed so that overexpressed polyphosphate would serve as a mercury accumulator; the mercury transport system would make the bacterial cell specifically accumulate mercury; and the intracellular accumulation process would allow the bioaccumulation system to be less sensitive to ambient conditions. The applicability of the new engineered bacteria in the environmental remediation of mercurials is evaluated and discussed in this review. <br>

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