Bacterial heavy metal transporter MerC increases mercury accumulation in Arabidopsis thaliana.
Project/Area Number |
21510088
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Environmental technology/Environmental materials
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Research Institution | Kitasato University |
Principal Investigator |
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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Keywords | メチル水銀トランスポーター / MerE / MerF / メチル水銀浄化 / ファイトレメディエーション / 土壌汚染浄化 |
Research Abstract |
This study evaluated the feasibility of transgenic Arabidopsis engineered to express the bacterial heavy metal transporter MerC for the phytoremediation of mercury pollution. MerC, MerC-SYP121, or MerC-AtVAM3 proteins were found to be expressed in leaf segments of transgenic plants using an anti-MerC antibody immunostaining method. Transgenic Arabidopsis plants expressing merC, merC-SYP121, and merC-AtVAM3 were more resistant to mercury and accumulated significantly more of this metal compared with wild-type Arabidopsis. These results demonstrated that the expression of the bacterial heavy metal transporter MerC can promote the transport and accumulation of mercury in transgenic Arabidopsis, which represents a method of improving plants for the phytoremediation of mercury pollution.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Expression of the bacterial heavy metal transporter MerC fused with a plant SNARE, SYP121, in Arabidopsis thaliana increases cadmium accumulation and tolerance2012
Author(s)
Kiyono M, Oka Y, Sone Y, Tanaka M, Nakamura R, Sato MH, Pan-Hou H, Sakabe K, Inoue K
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Journal Title
Planta
Volume: 235(4)
Pages: 841-850
Related Report
Peer Reviewed
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