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Severe ocular phenotypes in Rbp4-deficient mice in the C57BL/6 genetic background

https://doi.org/10.1038/labinvest.2016.39Get rights and content
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Abstract

Retinol-binding protein 4 (RBP4) is a specific carrier for retinol in the blood. In hepatocytes, newly synthesized RBP4 associates with retinol and transthyretin and is secreted into the blood. The ternary transthyretin–RBP4–retinol complex transports retinol in the circulation and delivers it to target tissues. Rbp4-deficient mice in a mixed genetic background (129xC57BL/6J) have decreased sensitivity to light in the b-wave amplitude on electroretinogram. Sensitivity progressively improves and approaches that of wild-type mice at 24 weeks of age. In the present study, we produced Rbp4-deficient mice in the C57BL/6 genetic background. These mice displayed more severe phenotypes. They had decreased a- and b-wave amplitudes on electroretinograms. In accordance with these abnormalities, we found structural changes in these mice, such as loss of the peripheral choroid and photoreceptor layer in the peripheral retinas. In the central retinas, the distance between the inner limiting membrane and the outer plexiform layer was much shorter with fewer ganglion cells and fewer synapses in the inner plexiform layer. Furthermore, ocular developmental defects of retinal depigmentation, optic disc abnormality, and persistent hyaloid artery were also observed. All these abnormalities had not recovered even at 40 weeks of age. Our Rbp4-deficient mice accumulated retinol in the liver but it was undetectable in the serum, indicating an inverse relation between serum and liver retinol levels. Our results suggest that RBP4 is critical for the mobilization of retinol from hepatic storage pools, and that such mobilization is necessary for ocular development and visual function.

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Supplementary Information accompanies the paper on the Laboratory Investigation website

Jingling Shen and Dan Shi: These authors contributed equally to this work.

Retinol binding protein 4 (RBP4) is a specific carrier for retinol in the blood. The authors produced Rbp4-deficient mice on a C57BL/6 genetic background. These mice display more severe and longer lasting retinal structural abnormalities than an older model using mice with a mixed genetic background. Furthermore, with the new model they show that RBP4 is critical for the mobilization of retinol from hepatic storage pools, and that such mobilization is necessary for ocular development and visual function.

Supplementary information: The online version of this article (doi:10.1038/labinvest.2016.39) contains supplementary material, which is available to authorized users.