photoreceptor cell sheet derived from mouse iPS cells
Project/Area Number |
24659769
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Ophthalmology
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Research Institution | St. Marianna University School of Medicine |
Principal Investigator |
UEDA Yuji 聖マリアンナ医科大学, 医学部, 講師 (00223470)
|
Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Noboru 聖マリアンナ医科大学, 医学部, 教授 (40235982)
ARIMITSU Nagisa 聖マリアンナ医科大学, 医学部, 助教 (40408688)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | iPS細胞 / 網膜 / 視細胞 / ロドプシン / 転写因子 / 桿体視細胞 / 錐体視細胞 / 網膜前駆細胞 / 再生医学 / オプシン |
Outline of Final Research Achievements |
The retinal tissue is derived from the central nervous system and does not have the regeneration ability. Thus once damaged, regeneration of retina is almost impossible. We generated retinal progenitor cell lines from mouse iPS cells transfected with pax6 cDNA, and by using the cloned retinal progenitors we made retinal cell sheet including rhodopsin positive photoreceptor cells. They made massive synaptic connection among them and the sheet looked resembling naïve retinal neuron layers. It could be clinically applicable for patients with damaged retina.
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Report
(4 results)
Research Products
(8 results)
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[Journal Article] Pax7 Gene Induction Rapidly Regulates Myocyte Homeostasis in Human Induced Pluripotent Stem (iPS) Cells.2015
Author(s)
Misawa H, Saito A, Shimizu J, Iinuma M, Shiratsuchi T, Fujiwara N, Takai K, Arimitsu N, Ueda Y, Wakisaka S, Suzuki T, Beppu M, Suzuki N.
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Journal Title
St. Marianna Medical Journal.
Volume: 5
Pages: 59-67
Related Report
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[Journal Article] Human iPS Cell Derived Neurons with Cortical Motor Neuron Phenotype are Relevant for Functional Recovery of Hemiplegic Mice with Injured Motor Cortex.2013
Author(s)
Kono T, Arimitsu N, Shimizu J, Takai K, Fujiwara N, Umehara T, Saito A, Ueda Y, Wakisaka S, Suzuki T, Hashimoto T, Tanaka Y, Suzuki N.
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Journal Title
St.Marianna Medical Journal
Volume: 4
Pages: 31-40
NAID
Related Report
Peer Reviewed
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[Journal Article] Transplantation of Motor Neurons Derived from Human iPS Cells into Total Transection Model of Spinal Cord Injury in Mice.2013
Author(s)
Umehara T, Arimitsu N, Iinuma M, Shimizu J, Takai K, Fujiwara N, Saito A, Kono T, Ueda Y, Wakisaka S, Suzuki T, Moroe Beppu, Suzuki N.
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Journal Title
St.Marianna Medical Journal
Volume: 4
Pages: 21-30
NAID
Related Report
Peer Reviewed
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[Journal Article] IGFII/Akt Signaling Regulates Myocyte Homeostasis in Human Induced Pluripotent Stem (iPS) Cells.2013
Author(s)
Saito A, Shimizu J, Fujiwara N, Takai K, Arimitsu N, Umehara T, Kono T, Misawa H, Ueda Y, Wakisaka S, Suzuki T, Moroe Beppu, Suzuki N.
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Journal Title
St.Marianna Medical Journal
Volume: 4
Pages: 41-48
Related Report
Peer Reviewed
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[Journal Article] Restoration of spatial memory dysfunction of human APP transgenic mice by transplantation of neurons derived from human iPS cells.2013
Author(s)
Fujiwara N, Shimizu J, Takai K, Arimitsu N, Saito A, Kono T, Umehara T, Ueda Y, Wakisaka S, Suzuki T, Suzuki N.
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Journal Title
Neuroscience Letters
Volume: 557
Pages: 129-134
Related Report
Peer Reviewed
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