Molecular mechanisms of the restoration of human APP transgenic mouse cognitive dysfunction after transplant of human iPS cell-derived neural cells
Project/Area Number |
26501005
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Regenerative medicine
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Research Institution | St. Marianna University School of Medicine |
Principal Investigator |
Arimitsu Nagisa 聖マリアンナ医科大学, 医学部, 助教 (40408688)
|
Co-Investigator(Kenkyū-buntansha) |
藤原 成芳 聖マリアンナ医科大学, 医学部, 助教 (50365425)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 再生医療 / 移植 / 認知症 / 遺伝子発現 / iPS / 記憶 / ヒトiPS細胞 |
Outline of Final Research Achievements |
We examined the molecular mechanisms involved in the alleviation of cognitive dysfunction in transplanted Alzheimer's disease model mice . Neural cells derived from hiPS cells were transplanted to the hilus of the dentate gyrus. After transplant, mice showed improvement in cognitive function. Human choline acetyltransferase (ChAT)-positive cholinergic neurons and small number of vesicular GABA transporter (VGAT)-positive neurons were distributed throughout the cortex of the grafted mice. Human and mouse ChAT and GABA-positive neurons and their receptors of both human origin and mouse origin were significantly increased in the cortex and hippocampus. The receptor-positive neurons expressed phosphorylated Akt and c-fos in the cortex. And we also confirmed that paracrine factors such as Reelin expressed in the injected area. These suggest that the grafted and host neurons may form positive feedback loops via neurotransmitter secretion in both the cerebral cortex and hippocampus.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Human iPS cell derived neural cell sheets exhibit mature neural and extendable scaffold functions and promote recovery in injured mouse spinal cords.2016
Author(s)
Arimitsu N, Shimizu J, Iinuma M, Umehara T, Fujiwara N, Takai K, Wakisaka S, Hirotsu C, Suzuki T, Beppu M, Niki H, Suzuki N.
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Journal Title
J Stem Cell Res Med.
Volume: 1
Issue: 2
Pages: 41-47
DOI
Related Report
Open Access
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