Identification of cancer initiating cell factors and an attempt of drug development
Project/Area Number |
26670625
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Respiratory surgery
|
Research Institution | Nagoya University |
Principal Investigator |
SUZUKI Motoshi 名古屋大学, 医学(系)研究科(研究院), 講師 (80236017)
|
Co-Investigator(Kenkyū-buntansha) |
FUKUI Takayuki 名古屋大学, 医学部附属病院, 病院講師 (70463198)
WAKAHARA Keiko 名古屋大学, 医学部附属病院, 病院講師 (00631433)
KATO Sei-ichi 名古屋大学, 大学院医学系研究科, 講師 (30584669)
Nishida Atsushi 千葉大学, 大学院薬学研究院, 教授 (80130029)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 肺がん / がん幹細胞 / 低分子化合物 / 肺癌 / 抗癌剤 / 分子標的治療薬 |
Outline of Final Research Achievements |
We collected clinical lung cancer specimens and transplanted each of in the immune-deficient mice. Xenografts were recovered after tumor formation, collagenase-treated, and sorted for CD166 positive population. In a cell line, the CD166 positive cells successfully formed secondary tumors in mice. We performed database analysis to figure out a gene candidate which may contribute to the cancer initiating cell nature. By using the above cell line, we established a stable cell line that is associated with a knockdown of this gene, and inoculated thousands of cells in mice. Results are forthcoming. We also screened for an inhibitor against this gene product. Among the 2000 candidates, we found a compound that inhibits the activity. To decrease the EC50 value, we started optimization of this compound.
|
Report
(3 results)
Research Products
(16 results)
-
-
[Journal Article] Resveratrol-induced transcriptional up-regulation of ASMase (SMPD1) of human leukemia and cancer cells.2016
Author(s)
Mizutani N, Omori Y, Kawamoto Y, Sobue S, Ichihara M, Suzuki M, Kyogashima M, Nakamura M, Tamiya-Koizumi K, Nozawa Y, Murate T.
-
Journal Title
Biochem Biophys Res Commun
Volume: 470
Issue: 4
Pages: 851-856
DOI
Related Report
Peer Reviewed
-
[Journal Article] ROR1 sustains caveolae and survival signaling as a scaffold of cavin-1 and caveolin-12016
Author(s)
Yamaguchi T, Lu C, Yanagisawa K, Can Lu, Usukura J, Cheng J, Hotta N, Shimada Y, Ida R, Isomura H, Suzuki M, Fujimoto T, Takahashi T
-
Journal Title
Nat Commun
Volume: 7
Issue: 1
Pages: 10060-10060
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article] Increased Acid Ceramidase Expression depends on Upregulation of Androgen-dependent Deubiquitinases, USP2, in a Human Prostate Cancer Cell Line, LNCaP.2015
Author(s)
Naoki Mizutani, Minami Inoue, Yukari Omori, Hiromi Ito, Keiko Tamiya-Koizumi, Akira Takagi, Tetsuhito Kojima, Mitsuhiro Nakamura, Soichiro Iwaki, Masahiro Nakatochi, Motoshi Suzuki, Yoshinori Nozawa, Takashi Murate
-
Journal Title
J. Biochem.
Volume: 印刷中
Issue: 4
Pages: 309-319
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Increased SPHK2 transcription of human colon cancer cells in serum-depleted culture: the involvement of CREB transcription factor.2015
Author(s)
Mizutani N, Omori Y, Tanaka K, Ito H, Takagi A, Kojima T, Nakatochi M, Ogiso H, Kawamoto Y, Nakamura M, Suzuki M, Kyogashima M, Koizumi K, Nozawa Y, Murate T.
-
Journal Title
J Cell Biochem
Volume: 116
Issue: 10
Pages: 2227-2238
DOI
Related Report
Peer Reviewed
-
[Journal Article] Increased Acid Ceramidase Expression depends on Upregulation of Androgen-dependent Deubiquitinases, USP2, in a Human Prostate Cancer Cell Line, LNCaP2015
Author(s)
Mizutani N, Inoue M, Omori Y, Ito H, Tamiya-Koizumi K, Takagi A, Kojima T, Nakamura M, Iwaki S, Nakatochi M, Suzuki M, Nozawa Y, and Murate M
-
Journal Title
J Biochem
Volume: in press
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Phosphorylated Sp1 is the regulator of DNA-PKcs and DNA ligase IV transcription of daunorubicin-resistant leukemia cell lines.2014
Author(s)
Nishida Y, Mizutani N, Inoue M, Omori Y, Tamiya-Koizumi K, Takagi A, Kojima T, Suzuki M, Nozawa Y, Minami Y, Ohnishi K, Naoe T, Murate T.
-
Journal Title
Biochim Biophys Acta.
Volume: 1839
Issue: 4
Pages: 265-274
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Lung adenocarcinoma subtypes definable by lung development-related miRNA expression profiles in association with clinicopathologic features2014
Author(s)
Arima C, Kajino T, Tamada Y, Imoto S, ShimadaY, Nakatochi, M, Suzuki M, Isomura H, Yatabe Y, Yamaguchi T, Yanagisawa K, Miyano S, Takahashi T
-
Journal Title
Carcinogenesis
Volume: 35
Issue: 10
Pages: 2224-2231
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
-
-