Establishment of human cell lines for blood-tissue barrier and application to elucidate mechanism of low-dose anti-cancer drugs
Project/Area Number |
21590175
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Medical pharmacy
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Research Institution | Keio University |
Principal Investigator |
NAKASHIMA Emi 慶應義塾大学, 薬学部, 教授 (90115254)
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Co-Investigator(Kenkyū-buntansha) |
TOMI Masatoshi 慶應義塾大学, 薬学部, 准教授 (30334717)
NISHIMURA Tomohiro 慶應義塾大学, 薬学部, 助教 (40453518)
KOSE Noriko 慶應義塾大学, 薬学部, 研究員 (60348612)
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Co-Investigator(Renkei-kenkyūsha) |
SAI Yoshimichi 金沢大学, 大学病院, 准教授 (40262589)
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Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 薬物動態 / 代謝学 / 血管内皮前駆細胞 / 壁細胞 / bFGF / SMA / ケモタキシス / 管腔形成能 / 血管新生阻害 / 抗がん薬 / CD133 / 抗がん剤 / 血管新生 / 腫瘍増殖抑制 / 骨髄由来血管内皮細胞 / 遊走能 / タキサン系抗がん剤 / 微小血管密度低下 |
Research Abstract |
In formation process of a blood-tissue barrier, there is an interaction between specific tissue cells and bone marrow(BM)-derived endothelial progenitor cells(EPC). An assay system using the established cell lines from the specific tissue is desirable to obtain reproducible analysis. By using an established bone marrow-derived vascular endothelium precursor cell line, TR-BME, docetaxel and paclitaxel directly inhibited EPC-initiated vasculogenesis at low(non-cytotoxic) concentrations, causing suppression of tumor growth.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Differential Expression of Ezrin and CLP36 in the Two Layers of Syncytiotrophoblast in Rats2010
Author(s)
Higuchi K, Iizasa H, Sai Y, Horieya S, Lee K-E, Wada M, Deguchi M, Nishimura T, Wakayama T, Tamura A, Tsukita S, Kose N, Kang Y-S, Nakashima E.
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 33
Issue: 8
Pages: 1400-1406
DOI
NAID
ISSN
0918-6158, 1347-5215
Related Report
Peer Reviewed
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[Journal Article] Proton-coupled erythromycin antiport at rat blood-placenta barrier2010
Author(s)
Sai Y, Nishimura T, Ochi K, Tanaka N, Takagi A, Tomi M, Kose N, Kobayashi Y, Miyakoshi N, Kitagaki S, Mukai C, Nakashima E.
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Journal Title
Drug Metab Dispos.
Volume: 38
Issue: 9
Pages: 1576-1581
DOI
Related Report
Peer Reviewed
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[Journal Article] Differential expression of ezrin and CLP36 in the two layers of syncytiotrophoblast in rats.2010
Author(s)
Higuchi K, Iizasa H, Sai Y, Horieya S, Lee K-E, Wada M, Deguchi M, Nishimura T, Wakayama T, Tamura A, Tsukita S, Kose N, Kang Y-S, Nakashima E.
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Journal Title
Biol Pharm Bull
Volume: 33
Pages: 1400-1406
NAID
Related Report
Peer Reviewed
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[Journal Article] Proton-coupled erythromycin antiport at rat blood-placenta barrier.2010
Author(s)
Sai Y, Nishimura T, Ochi K, Tanaka N, Takagi A, Tomi M, Kose N, Kobayashi Y, Miyakoshi N, Kitagaki S, Mukai C, Nakashima E.
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Journal Title
Drug Metab Dispos
Volume: 38
Pages: 1576-1581
Related Report
Peer Reviewed
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