Challenge to development of innovative method to control monocyte/macrophage lineages
Project/Area Number |
15K15386
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Pediatrics
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
Morio Tomohiro 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (30239628)
|
Research Collaborator |
OTSU Makoto 東京大学医科学研究所, 幹細胞プロセシング分野, 准教授
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 小児免疫 / アレルギー / 膠原病学 |
Outline of Final Research Achievements |
We established a method to differentiate hematopoietic stem cells into monocytes using peripheral blood mononuclear cell derived iPS cells. We further refined a method to induce differentiation of monocytes to dendritic cells and also attempted to induce monocytes into M1 macrophages or to M2 macrophages. In this study, we have identified a novel responsible gene, OAS1, for B/Mo/DC deficiency with alveolar proteinosis through whole exome analysis of affected individuals. iPS cells were established from the patient; and a capacity of the cells to differentiate to monocytes or to DC was examined. In parallel, we generated OAS1 knock in mice to delve into the mechanism of monocyte differentiation defect. Finally, we were successful in inducing Aspergillus fumigatus-specific and Candida albicans-specific T cells using known overlapping peptides and optimized cytokines.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Abnormal hematopoiesis and autoimmunity in human subjects with germline IKZF1 mutations.2017
Author(s)
Hoshino A, Okada S, Yoshida K, Nishida N, Okuno Y, Ueno H, Yamashita M, Okano T, Tsumura M, Nishimura S, Sakata S, Kobayashi M, Nakamura H, Kamizono J, Mitsui-Sekinaka K, Ichimura T, Ohga S, Nakazawa Y, Takagi M, Imai K, Shiraishi Y, Chiba K, Tanaka H, Miyano S, Ogawa S, Kojima S, Nonoyama S, Morio T, Kanegane H.
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Journal Title
J Allergy Clin Immunol.
Volume: -
Issue: 1
Pages: 390-400
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Hematopoietic Stem Cell Transplantation for XIAP Deficiency in Japan.2017
Author(s)
3.Ono S, Okano T, Hoshino A, Yanagimachi M, Hamamoto K, Nakazawa Y, Imamura T, Onuma M, Niizuma H, Sasahara Y, Tsujimoto H, Wada T, Kunisaki R, Takagi M, Imai K, Morio T, Kanegane H.
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Journal Title
J Clin Immunol.
Volume: 37
Issue: 1
Pages: 85-91
DOI
Related Report
Peer Reviewed / Open Access
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