Development of a predictive system of the sensitivity for L-asparaginase in tumor by monoclonal antibody
Project/Area Number |
24590713
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory medicine
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Research Institution | Aichi Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
YOKOI TOYOHARU 愛知医科大学, 医学部, 教授 (40200886)
TSURUSAWA MASAHITO 愛知医科大学, 医学部, 教授 (90172064)
KATO HIDEFUMI 愛知医科大学, 医学部, 教授 (40298584)
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Co-Investigator(Renkei-kenkyūsha) |
HAMAKUBO TAKAO 東京大学, 先端科学技術研究センター, 教授 (90198797)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | 分子標的療法 / アミノ酸要求性 / 選択毒性 / 抗がん剤 / 細胞死 / 酵素製剤 / 感受性予測 / アミノ酸代謝 / 国際情報交換 / 選択的毒性 |
Outline of Final Research Achievements |
Due to the lack of rapid upregulation of asparagine synthetase (ASNS) in ALL cells, they are preferentially killed by ASNase-induced asparagine depletion. The purpose of this study was to examine the usefulness of a rapid flow cytometric assay that measure ASNS protein in whole cells to predict sensitivity and resistance to ASNase. ASNS expression evaluated by cytosolic flow cytometry with the Z5808 monoclonal antibody (McAb) (Hybridoma, 2012) significantly correlated with ASNS activity in leukaemia cell lines. The ASNS protein level was inversely correlated with sensitivity to ASNase in both leukaemia cell lines and primary leukaemia cells. We have shown that ASNS protein expression measured by flow cytometry assay strongly predicts ASNase activity in leukaemia cells and that lower levels of ASNS protein found in AML-M1, M5 and M7 subtypes. The utility of using flow cytometry to measure ASNS levels in clinical samples and the future potential of this methodology is promising.
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Report
(5 results)
Research Products
(19 results)
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[Journal Article] Hematopoietic stem cell transplantation for patients with acute lymphoblastic leukemia and Down syndrome2015
Author(s)
Goto, H. Kaneko, T. Shioda, Y. Kajiwara, M. Sakashita, K. Kitoh, T. Hayakawa, A. Miki, M. Kato, K. Ogawa, A. Hashii, Y. Inukai, T. Kato, C. Sakamaki, H. Yabe, H. Suzuki, R. Kato, K.
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Journal Title
Pediatr Blood Cancer
Volume: 62
Issue: 1
Pages: 148-152
DOI
Related Report
Peer Reviewed
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[Journal Article] Late-Onset Combined Immunodeficiency with a Novel IL2RG Mutation and Probable Revertant Somatic Mosaicism.2015
Author(s)
Okuno Y, Hoshino A, Muramatsu H, Kawashima N, Wang X, Yoshida K, Wada T, Gunji M, Toma T, Kato T, Shiraishi Y, Iwata A, Hori T, Kitoh T, Chiba K, Tanaka H, Sanada M, Takahashi Y, Nonoyama S, Ito M, Miyano S, Ogawa S, Kojima S, Kanegane H.
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Journal Title
J Clin Immunol
Volume: 35
Issue: 7
Pages: 610-614
DOI
Related Report
Peer Reviewed
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[Journal Article] Reduced intensity conditioning in allogeneic stem cell transplantation for AML with Down syndrome.2014
Author(s)
Muramatsu H, Sakaguchi H, Taga T, Tabuchi K, Adachi S, Inoue M, Kitoh T, Suminoe A, Yabe H, Azuma E, Shioda Y, Ogawa A, Kinoshita A, Kigasawa H, Osugi Y, Koike K, Kawa K, Kato K, Atsuta Y, Kudo K.
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Journal Title
Pediatr Blood Cancer
Volume: 61
Issue: 5
Pages: 925-927
DOI
Related Report
Peer Reviewed
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[Presentation] Flow Cytometric Detection of Asparagine Synthetase Protein in Leukemia Cells; Indication for L- Asparaginase Therapy2014
Author(s)
Toshiyuki Kitoh, Kou Suchan Gao Siqiang, Hidefumi Kato, Kenji Miyata, Yasuto Shimomura, Toshinori Hori, Kimiyoshi Sakaguchi, Yasuo Horikoshi, Kentarou Ohki, Katsuyoshi Koh, and Takao Hamakubo.
Organizer
56th ASH Annual Meeting and Exposition
Place of Presentation
San Francisco, CA, USA
Year and Date
2014-12-06 – 2014-12-09
Related Report
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[Presentation] DEVELOPMENT OF NEW CLASSIFICATION CRITERIA FOR MACROPHAGE ACTIVATION SYNDROME COMPLICATING SYSTEMIC JUVENILE IDIOPATHIC ARTHRITIS2014
Author(s)
Minoia F, Davi S, Bovis F, Pistorio A, Arico M, Avcin T, Behrens E, FD, Filipovich L, Grom A,Henter JI, Horne A, Ilowite N,Jordan M, Khubchandani R,Kitoh T, et.al
Organizer
the 21st European Pediatric Rheumatology Congress
Place of Presentation
Belgrade, Serbia
Year and Date
2014-09-17 – 2014-09-21
Related Report
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[Presentation] Hematopoietic Cell Transplantation for Patients with Acute Lymphoblastic Leukemia and Down Syndrome.2014
Author(s)
Hiroaki Goto, Takashi Kaneko, Yoko Shioda, Michiko Kajiwara, Kazuo Sakashita, Toshiyuki Kitoh, Akira Hayakawa, Mizuka Miki, Hisashi Sakamaki, Hiromasa Yabe, Ritsuro Suzuki, Kohji Kato
Organizer
8th SIOP-Asia Congress
Place of Presentation
Seoul, Korea
Year and Date
2014-04-15 – 2014-04-17
Related Report
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