Next generation sequencing analysis of transient abnormal myelopoiesis and acute megakaryoblastic leukemia in Down syndrome
Project/Area Number |
26461598
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pediatrics
|
Research Institution | Gunma Institute of Public Health and Environmental Sciences |
Principal Investigator |
PARK Myoung-ja 群馬県衛生環境研究所, 研究企画係, 研究員 (50450375)
|
Co-Investigator(Kenkyū-buntansha) |
林 泰秀 群馬県衛生環境研究所, 研究企画係, 研究員 (30238133)
大木 健太郎 国立研究開発法人国立成育医療研究センター, 小児血液・腫瘍研究部, 室長 (50400966)
外松 学 群馬県衛生環境研究所, 研究企画係, 研究員 (70251113)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 遺伝子 / ゲノム / マイクロアレイ / 癌 / ダウン症候群 / 一過性骨髄異常増殖症 / 急性巨核芽球性白血病 / エクソーム解析 / サイトカイン / ダウン症 |
Outline of Final Research Achievements |
Cytokine analyses of 27 serum cytokines in 126 patients with transient abnormal myelopoiesis (TAM) in Down syndrome (DS), revealed high value of IL-1β, IL-2, and IL-17, but specific cytokine for TAM was not found. Eight patients having GATA1 mutation were found in 250 patients with acute myeloid leukemia (AML) and association of the 8 patients with clinical features was examined. Cohesin complex, EZH2, and JAK2, which were frequently found by whole-exome sequencing in DS-acute megakaryoblastic leukemia (AMKL) were not found in any patients with non-DS AMKL. No CBFA2T3-GLIS2 or OTT-MAL chimeric RNAs were found in TAM and DS-AMKL, suggesting that DS-AMKL was biologically different disease entity from non-DS-AMKL.
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Report
(4 results)
Research Products
(53 results)
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[Journal Article] ASXL2 Mutations are Frequently Found in Pediatric AML Patients with t(8;21)/RUNX1-RUNX1T1 and Associated with a Better Prognosis.2017
Author(s)
Yamato G, Shiba N, Yoshida K, Shiraishi Y, Hara Y, Ohki K, Okubo J, Okuno H, Chiba K, Tanaka H, Kinoshita A, Moritake H, Kiyokawa N, Tomizawa D, Park MJ, Sotomatsu M, Taga T, Adachi S, Tawa A, Horibe K, Arakawa H, Miyano S, Ogawa S, Hayashi Y.
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Journal Title
Genes Chromosomes Cancer.
Volume: -
Issue: 5
Pages: 382-393
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Whole-exome sequencing reveals the spectrum of gene mutations and the clonal evolution patterns in paediatric acute myeloid leukaemia.2016
Author(s)
Shiba N, Yoshida K, Shiraishi Y, Okuno Y, Yamato G, Hara Y, Nagata Y, Chiba K, Tanaka H, Terui K, Kato M, Park MJ, Ohki K, Shimada A, Takita J, Tomizawa D, Kudo K, Arakawa H, Adachi S, Taga T, Tawa A, Ito E, Horibe K, Sanada M, Miyano S, Ogawa S, Hayashi Y.
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Journal Title
Br J Haematol.
Volume: 175
Issue: 3
Pages: 476-489
DOI
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Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] High PRDM16 expression identifies a prognostic subgroup of pediatric acute myeloid leukaemia correlated to FLT3-ITD, KMT2A-PTD, and NUP98-NSD1: the results of the Japanese Paediatric Leukaemia/Lymphoma Study Group AML-05 trial.2015
Author(s)
Shiba N, Ohki K, Kobayashi T, Hara Y, Yamato G, Tanoshima R, Ichikawa H, Tomizawa D, Park MJ, Shimada A, Sotomatsu M, Arakawa H, Horibe K, Adachi S, Taga T, Tawa A, Hayashi Y.
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Journal Title
Br J Haematol.
Volume: 172
Issue: 4
Pages: 581-91
DOI
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Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Early use of allogeneic hematopoietic stem cell transplantation for infants with MLL gene-rearrangement-positive acute lymphoblastic leukemia.2015
Author(s)
Koh K, Tomizawa D, Moriya Saito A, Watanabe T, Miyamura T, Hirayama M, Takahashi Y, Ogawa A, Kato K, Sugita K, Sato T, Deguchi T, Hayashi Y, Takita J, Takeshita Y, Tsurusawa M, Horibe K, Mizutani S, Ishii E.
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Journal Title
Leukemia
Volume: 29(2)
Issue: 2
Pages: 290-6
DOI
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Peer Reviewed / Open Access
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[Journal Article] 小児Ph-like ALL症例の表面マーカー、遺伝子発現解析2014
Author(s)
飯島 一智, 清河 信敬, 吉原 宏樹, 富田 理, 小林 健一郎, 福島 敬, 林 泰秀, 菊地 陽, 康 勝好, 真部 淳, 小原 明
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Journal Title
日本小児血液・がん学会雑誌
Volume: 51巻3号
Pages: 200-205
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Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Gene alterations involving the CRLF2-JAK pathway and recurrent gene deletions in Down syndrome-associated acute lymphoblastic leukemia in Japan.2014
Author(s)
Hanada I, Terui K, Ikeda F, Toki T, Kanezaki R, Sato T, Kamio T, Kudo K, Sasaki S, Takahashi Y, Hayashi Y, Inukai T, Kojima S, Koike K, Kosaka Y, Kobayashi M, Imaizumi M, Mitsui T, Hori H, Hara J, Horibe K, Nagai J, Goto H, Ito E.
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Journal Title
Genes Chromosomes Cancer
Volume: 53
Issue: 11
Pages: 902-910
DOI
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Peer Reviewed / Open Access
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[Journal Article] Acute myeloid leukaemia with myelodysplastic features in children2014
Author(s)
Kinoshita A, Miyachi H, Matsushita H, Yabe M, Taki T, Watanabe T, Saito AM, Tomizawa D, Taga T, Takahashi H, Matsuo H, Kodama K, Ohki K, Hayashi Y, Tawa A, Horibe K, Adachi S.
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Journal Title
Br J Haematol
Volume: 167
Issue: 1
Pages: 80-86
DOI
Related Report
Peer Reviewed
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[Journal Article] EVI1 overexpression is a poor prognostic factor in pediatric patients with mixed lineage leukemia-AF9 rearranged acute myeloid leukemia.2014
Author(s)
Matsuo H, Kajihara M, Tomizawa D, Watanabe T, Saito AM, Fujimoto J, Horibe K, Kodama K, Tokumasu M, Itoh H, Nakayama H, Kinoshita A, Taga T, Tawa A, Taki T, Shiba N, Ohki K, Hayashi Y, Yamashita Y, Shimada A, Tanaka S, Adachi S.
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Journal Title
Haematologica
Volume: 99
Issue: 11
Pages: 225-227
DOI
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[Journal Article] Outcome of children with relapsed acute myeloid leukemia following initial therapy under the AML99 protocol2014
Author(s)
Nakayama H, Tabuchi K, Tawa A, Tsukimoto I, Tsuchida M, Morimoto A, Yabe H, Horibe K, Hanada R, Imaizumi M, Hayashi Y, Hamamoto K, Kobayashi R, Kudo K, Shimada A, Miyamura T, Moritake H, Tomizawa D, Taga T, Adachi S.
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Journal Title
Int J Hematol
Volume: 100(2)
Issue: 2
Pages: 171-9
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Mutations of the GATA2 and CEBPA genes in paediatric acute myeloid leukaemia.2014
Author(s)
Shiba N, Funato M, Ohki K, Park MJ, Mizushima Y, Adachi S, Kobayashi M, Kinoshita A, Sotomatsu M, Arakawa H, Tawa A, Horibe K, Tsukimoto I, Hayashi Y.
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Journal Title
Brit J Haematol
Volume: 印刷中
Issue: 1
Pages: 142-159
DOI
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Peer Reviewed
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[Journal Article] Recurrent CDC25C mutations drive malignant transformation in FPD/AML2014
Author(s)
Yoshimi A, Toya T, Kawazu M, Ueno T, Tsukamoto A, Iizuka H, Nakagawa M, Nannya Y, Arai S, Harada H, Usuki K, Hayashi Y, Ito E, Kirito K, Nakajima H, Ichikawa M, Mano H, Kurokawa M
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Journal Title
Nature communications
Volume: 5
Issue: 1
Pages: 4770-4770
DOI
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Peer Reviewed / Open Access
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[Presentation] 乳児急性骨髄性白血病におけるMLL遺伝子再構成及びCBFA2T3-GLIS2による予後層別化(Novel risk stratification of infant acute myeloid leukemia using MLL-rearrangements and CBFA2T3-GLIS2)2016
Author(s)
原勇介, 柴徳生, 大木健太郎, 大和玄季, 朴明子, 富澤大輔, 滝智彦, 木下明俊, 清河信敬, 荒川浩一, 多和昭雄, 堀部敬三, 多賀崇, 足立壮一, 林泰秀
Organizer
第58回日本小児血液・がん学会学術集会
Place of Presentation
品川プリンスホテル
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[Presentation] 小児急性骨髄性白血病におけるRUNX1変異を有する患者の頻度と臨床的特徴(Frequency and clinical features of RUNX1 mutations in pediatric acute myeloid leukemia patients)2016
Author(s)
大和玄季, 柴徳生, 吉田健一, 原勇介, 大木健太郎, 大久保淳, 朴明子, 外松学, 清河信敬, 富澤大輔, 多賀崇, 足立壮一, 多和昭雄, 堀部敬三, 荒川浩一, 小川誠司, 林泰秀
Organizer
第58回日本小児血液・がん学会学術集会
Place of Presentation
品川プリンスホテル
Related Report
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[Presentation] 一過性異常骨髄増殖症におけるGATA1遺伝子変異 JPLSG TAM-10登録症例の解析(GATA1 mutation status in infants with transient abnormal myelopoiesis: A report from the JPLSG TAM-10 study)2016
Author(s)
照井君典, 土岐力, 濱麻人, 村松秀城, 長谷川大輔, 朴明子, 岩本彰太郎, 多賀崇, 柳澤龍, 康勝好, 林泰秀, 足立壯一, 水谷修紀, 渡邉健一郎, 伊藤悦朗
Organizer
第58回日本小児血液・がん学会学術集会
Place of Presentation
品川プリンスホテル
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[Presentation] Analysis of GATA1 Mutations in Down Syndrome Infants with Transient Abnormal Myelopoiesis and Clinical Impacts of GATA1 Mutation Types: A Report from the JPLSG TAM-10 Study2016
Author(s)
Terui K, Toki T, Hama A, Muramatsu H, Hasegawa D, Park MJ, Iwamoto S, Taga T, Yanagisawa R, Koh, Saito A, Horibe K, Hayashi Y, Adachi A, Mizutani S, Watanabe K, Ito E
Organizer
58th ASH Annual Meeting and Exposition
Place of Presentation
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Related Report
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[Presentation] Molecular features and their clinical impact in infant acute myeloid leukemia2016
Author(s)
原勇介, 柴徳生, 大木健太郎, 大和玄季, 朴明子, 富澤大輔, 滝智彦, 木下明俊, 荒川浩一, 多和昭雄, 堀部敬三, 多賀崇, 足立壯一, 林泰秀
Organizer
第78回日本血液学会学術集会
Place of Presentation
パシフィコ横浜
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[Presentation] 小児T細胞性ALLにおける既知遺伝子異常の解析:TCCSG-ALL研究, 口頭2016
Author(s)
大木健太郎, 清河信敬, 朴明子, 小林健一郎, 福島敬, 康勝好, 花田良二, 林泰秀, 真部淳, 小原明
Organizer
第119回日本小児科学会学術集会
Place of Presentation
ロイトン札幌
Related Report
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[Presentation] Clinical features of patients with ASXL1 and ASXL2 mutations in pediatric acute myeloid leukemia2016
Author(s)
13.大和玄季, 柴徳生, 吉田健一, 大木健太郎, 大久保淳, 原勇介, 木下明俊, 富澤大輔, 外松学, 多賀崇, 足立壯一, 多和昭雄, 堀部敬三, 荒川浩一, 小川誠司, 林泰秀
Organizer
第78回日本血液学会学術集会
Place of Presentation
パシフィコ横浜
Related Report
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[Presentation] Prospective study of 168 infants with transient abnormal myelopoiesis with Down syndrome: Japan Pediatric Leukemia/Lymphoma Study Group, TAM-10 study2015
Author(s)
Muramatsu H, Watanabe T, Hasegawa D, Park MJ, Iwamoto S, Taga T, Ito E, Toki T, Terui K, Yanagisawa R, Koh K, Saito M, Horibe K, Hayashi Y, Adachi S, Mizutani S, Watanabe K
Organizer
57th ASH Annual Meeting and Exposition
Place of Presentation
Orland
Year and Date
2015-12-05
Related Report
Int'l Joint Research
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[Presentation] A Combination of EVI1 and PRDM16 Expression Clarified the Clinical Features of Intermediate/High Risk Patients in Pediatric Acute Myeloid Leukemia.2015
Author(s)
Yamato G, Shiba N, Hara Y, Park M, Sotomatsu M, Ohki K, Ichikawa H, Tomizawa D, Taga T, Horibe K, Tawa A, Arakawa H, Adachi S, Hayashi Y.
Organizer
57th ASH Annual Meeting and Exposition
Place of Presentation
Orland
Year and Date
2015-12-05
Related Report
Int'l Joint Research
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[Presentation] Detection of Novel Pathogenic Gene Rearrangements in Pediatric Acute Myeloid Leukemia By RNA Sequencing.2015
Author(s)
Shiba N, Yoshida K, Shiraishi Y, Hara Y, Yamato G, Kaburaki T, Sanada M, Oki K, Tomizawa D, Sotomatsu M, Arakawa H, Horibe K, Park M, Taga T, Adachi S, Tawa A, Miyano S, Ogawa S, Hayashi Y.
Organizer
57th ASH Annual Meeting and Exposition
Place of Presentation
Orland
Year and Date
2015-12-05
Related Report
Int'l Joint Research
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[Presentation] High BMP2 Expression Is a Poor Prognostic Factor and a Good Candidate to Identify CBFA2T3-GLIS2-like High-Risk Subgroup in Pediatric Acute Myeloid Leukemia.2015
Author(s)
Hara Y, Yamato G, Shiba N, Ohki K, Park M, Tomizawa D, Taki T, Taga T, Arakawa H, Tawa A, Horibe K, Adachi S, Hayashi Y.
Organizer
57th ASH Annual Meeting and Exposition
Place of Presentation
Orland
Year and Date
2015-12-05
Related Report
Int'l Joint Research
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[Presentation] The Prognostic Impact of High MEL1 Gene Expression in Pediatric Acute Myeloid Leukemia2014
Author(s)
Shiba N, Ohki K, Hara Y, Yamato G, Park M, Ichikawa H, Kobayashi T, Tomizawa D, Sotomatsu M, Arakawa H, Horibe K, Taga T, Adachi S, Tawa A, Hayashi Y
Organizer
56th ASH Annual Meeting and Exposition
Place of Presentation
San Francisco
Year and Date
2014-12-06 – 2014-12-09
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[Presentation] Poor Prognosis Associated with FAB Subtypes M4 and M5 in Japanese Pediatric Acute Myeloid Leukemia Patients with FLT3-ITD2014
Author(s)
Hara Y, Shiba N, Ohki K, Park M, Shimada A, Tomizawa D, Moriya Saito M, Fujimoto J, Taki T, Kinoshita A, Taga T, Arakawa H, Tawa A, Horibe K, Adachi S, Hayashi Y
Organizer
56th ASH Annual Meeting and Exposition
Place of Presentation
San Francisco
Year and Date
2014-12-06 – 2014-12-09
Related Report
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[Presentation] Clinical Features of Patients with ASXL1 and ASXL2 Mutations in Pediatric Acute Myeloid Leukemia2014
Author(s)
Yamato G, Shiba N, Yoshida K, Ohki K, Park M, Hara Y, Tomizawa D, Sotomatsu M, Taga T, Adachi S, Tawa A, Horibe K, Arakawa H, Ogawa S, Hayashi Y
Organizer
56th ASH Annual Meeting and Exposition
Place of Presentation
San Francisco
Year and Date
2014-12-06 – 2014-12-09
Related Report
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[Presentation] ダウン症における一過性異常骨髄増殖症の形態学的特徴 JPLSG TAM-10形態中央診断の解析(Morphological characteristics of transient abnormal myelopoiesis (TAM) in Down syndrome: Analysis of central morphology review from the JPLSG TAM-10 study)2014
Author(s)
濱 麻人, 村松 秀城, 長谷川 大輔, 朴 明子, 岩本 彰太郎, 多賀 崇, 伊藤 悦朗, 柳沢 龍, 康 勝好, 林 泰秀, 足立 壮一, 水谷 修紀, 渡邉 健一郎
Organizer
第56回日本小児血液・がん学会学術集会
Place of Presentation
岡山
Year and Date
2014-11-28 – 2014-11-30
Related Report
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[Presentation] 小児AMLにおけるIKZF1欠失の頻度と予後解析 JPLSG AML-05(Frequency and prognosis of IKZF1 deletions in pediatric acute myeloid leukemia: The Japanese Pediatric Leukemia/Lymphoma Study Group (JPLSG) AML-05 Trial)2014
Author(s)
大木 健太郎, 朴 明子, 原 勇介, 柴 徳生, 外松 学, 富澤 大輔, 多賀 崇, 齋藤 明子, 藤本 純一郎, 多和 昭雄, 堀部 敬三, 足立 壮一, 林 泰秀.
Organizer
第56回日本小児血液・がん学会学術集会
Place of Presentation
岡山
Year and Date
2014-11-28 – 2014-11-30
Related Report
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[Presentation] 小児急性骨髄性白血病におけるASXL1、ASXL2遺伝子変異と臨床像(Clinical features of Patients with ASXL1 and ASXL2 mutations in pediatric acute myeloid leukemia)2014
Author(s)
大和 玄季, 柴 徳生, 吉田 健一, 大木 健太郎, 朴 明子, 原 勇介, 外松 学, 多賀 崇, 富澤 大輔, 足立 壮一, 多和 昭雄, 堀部 敬三, 荒川 浩一, 小川 誠司, 林 泰秀
Organizer
第56回日本小児血液・がん学会学術集会
Place of Presentation
岡山
Year and Date
2014-11-28 – 2014-11-30
Related Report
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[Presentation] CSF3R and CALR mutations and cytogenetic findings in pediatric myeloid malignancies2014
Author(s)
Sano H, Ohki K, Park M, Shiba N, Hara Y, Sotomatsu M, Tomizawa D, Taga T, Kiyokawa N, Tawa A, Horibe K, Adachi A, Hayashi Y
Organizer
第76回日本血液学会学術集会
Place of Presentation
大阪
Year and Date
2014-10-31 – 2014-11-02
Related Report
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[Presentation] Pediatric AML with FLT3-ITD /WT, NUP98-NSD1, NPM1 and WT1 mutations affected the clinical outcome2014
Author(s)
Shimada A, Yamashita Y, Tomizawa D, Shiba N, Tawa A, Watanabe T, Yokozawa T, Kudo K, Taga T, Iwamoto S, Terui K, Moritake H, Kinoshita A, Takahashi H, Nakayama H, Koh K, Goto H, Kosaka Y, Saito A, Fujimoto J, Horibe K, Hara Y, Ohki K, Hayashi Y, Adachi A
Organizer
第76回日本血液学会学術集会
Place of Presentation
大阪
Year and Date
2014-10-31 – 2014-11-02
Related Report
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[Presentation] The prognostic impact of high EV1-related genes expression in pediatric acute myeloid leukemia2014
Author(s)
Shiba N, Hara Y, Ohki K, Yamato G, Park M, Kobayashi T, Ichikawa H, Tomizawa D, Taki T, Shimada A, Sotomatsu M, Arakawa H, Horibe H, Adachi S, Tawa A, Hayashi Y
Organizer
第76回日本血液学会学術集会
Place of Presentation
大阪
Year and Date
2014-10-31 – 2014-11-02
Related Report
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[Presentation] 神経芽腫大規模検体におけるgenetic landscapeと予後解析2014
Author(s)
瓜生 久美子, 西村 力, 吉田 健一, 関 正史, 星野 論子, 吉田 美沙, 加藤 元博, 樋渡 光輝, 林 泰秀, 田尻 達郎, 中川原 章, 小川 誠司, 滝田 順子
Organizer
第73回日本癌学会学術総会
Place of Presentation
横浜
Year and Date
2014-09-25 – 2014-09-27
Related Report
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[Presentation] 小児急性骨髄性白血病におけるGATA2変異の解析2014
Author(s)
原 勇介, 柴 徳生, 大木 健太郎, 朴 明子, 足立 壮一, 多賀 崇, 荒川 浩一, 多和 昭雄, 堀部 敬三, 林 泰秀
Organizer
第117回日本小児科学会学術集会
Place of Presentation
名古屋
Year and Date
2014-04-11 – 2014-04-13
Related Report
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[Presentation] 小児急性骨髄性白血病における分子生物学的背景を用いた新たな治療層別化への試み2014
Author(s)
柴 徳生, 吉田 健一, 大木 健太郎, 金澤 崇, 足立 壮一, 多和 昭雄, 伊藤 悦朗, 荒川 浩一, 小川 誠司, 林 泰秀, JPLSG AML委員会
Organizer
第117回日本小児科学会学術集会
Place of Presentation
名古屋
Year and Date
2014-04-11 – 2014-04-13
Related Report
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[Presentation] 芽球割合の低いTAMにおける臨床像の解析2014
Author(s)
朴 明子, 外松 学, 新井 心, 大木 健太郎, 小林 富男, 丸山 憲一, 鮫島 希代子, 林 泰秀
Organizer
第117回日本小児科学会学術集会
Place of Presentation
名古屋
Year and Date
2014-04-11 – 2014-04-13
Related Report
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[Presentation] ダウン症における一過性異常骨髄増殖症の形態学的特徴 JPLSG TAM-10形態中央診断の解析(Morphological characteristics of transient abnormal myelopoiesis (TAM) in Down syndrome: Analysis of central morphology review from the JPLSG TAM-10 study)2014
Author(s)
濱 麻人, 村松 秀城, 長谷川 大輔, 朴 明子, 岩本 彰太郎, 多賀 崇, 伊藤 悦朗, 柳沢 龍, 康 勝好, 林 泰秀, 足立 壮一, 水谷 修紀, 渡邉 健一郎
Organizer
第117回日本小児科学会学術集会
Place of Presentation
名古屋
Year and Date
2014-04-11 – 2014-04-13
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[Presentation] Recurrent CDC25C mutations drive malignant transformation in FPD/AML2014
Author(s)
Yoshimi A, Toya T, Kawazu M, Ueno T, Tsukamoto A, Iizuka1 H, Nakagawa M, Nannya Y, Arai S, Ichikawa M, Harada H, Usuki K, Hayashi Y, Ito E, Kirito K, Nakajima H, Mano H, Kurokawa M
Organizer
AACR Annual Meeting 2014
Place of Presentation
San Diego
Year and Date
2014-04-05 – 2014-04-09
Related Report