TFE3 Is a bHLH-ZIP-type Transcription Factor that Regulates the Mammalian Golgi Stress Response

  • Taniguchi Mai
    Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
  • Nadanaka Satomi
    Department of Biochemistry, Kobe Pharmaceutical University
  • Tanakura Soichiro
    Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
  • Sawaguchi Shogo
    Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
  • Midori Sachiko
    Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
  • Kawai Yumeto
    Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
  • Yamaguchi Shogo
    Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
  • Shimada Yui
    Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
  • Nakamura Yuki
    Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
  • Matsumura Yasuyo
    Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
  • Fujita Natsumi
    Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
  • Araki Naoko
    Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
  • Yamamoto Mayu
    Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
  • Oku Masaya
    Department of Biophysics, Graduate School of Science, Kyoto University
  • Wakabayashi Sadao
    Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo
  • Kitagawa Hiroshi
    Department of Biochemistry, Kobe Pharmaceutical University
  • Yoshida Hiderou
    Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo Department of Biophysics, Graduate School of Science, Kyoto University

Search this article

Abstract

The Golgi stress response is a mechanism by which, under conditions of insufficient Golgi function (Golgi stress), the transcription of Golgi-related genes is upregulated through an enhancer, the Golgi apparatus stress response element (GASE), in order to maintain homeostasis in the Golgi. The molecular mechanisms associated with GASE remain to be clarified. Here, we identified TFE3 as a GASE-binding transcription factor. TFE3 was phosphorylated and retained in the cytoplasm in normal growth conditions, whereas it was dephosphorylated, translocated to the nucleus and activated Golgi-related genes through GASE under conditions of Golgi stress, e.g. in response to inhibition of oligosaccharide processing in the Golgi apparatus. From these observations, we concluded that the TFE3-GASE pathway is one of the regulatory pathways of the mammalian Golgi stress response, which regulates the expression of glycosylation-related proteins in response to insufficiency of glycosylation in the Golgi apparatus.

Journal

Citations (8)*help

See more

References(71)*help

See more

Related Projects

See more

Details

Report a problem

Back to top