The development and demonstration of methodology to elucidate the sugar chain function and glycosyltransferase knockout mouse phenotype.
Project/Area Number |
23500493
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory animal science
|
Research Institution | Kanazawa University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
ASANO Masahide 金沢大学, 学際科学実験センター, 教授 (50251450)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 発生工学 / 遺伝子改変動物 / 糖鎖生物学 |
Research Abstract |
From behavioral analyses of beta 1, 4-galactosyltransferase-2 (B4GalT-2) gene knockout mice, we have found that galactose sugar chains synthesized by B4GalT-2 have a function in the central nervous system. Therefore, we tried to generate novel knock-in mice in order to identify cells and brain regions where B4GalT-2 is involved, to obtain clues identifying functional carbohydrate molecules. We constructed two kinds of targeting vectors, one for monitoring B4GalT-2 expression by lacZ gene and the other for recovering B4GalT-2 expression conditionally. We are trying to isolate desired ES clones.
|
Report
(4 results)
Research Products
(8 results)
-
[Journal Article] EXTL2, a member of EXT family of tumor suppressors, controls glycosaminoglycan biosynthesis in a xylose kinase-dependent manner2013
Author(s)
Nadanaka, S., Zhou, S., Kagiyama, S., Shoji, N., Sugahara, K., Sugihara, K., Asano, M.and Kitagawa, H.
-
Journal Title
J. Biol. Chem
Volume: 288
Issue: 13
Pages: 9321-9333
DOI
Related Report
Peer Reviewed
-
[Journal Article] Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach2012
Author(s)
Sugahara, D., Kaji, H., Sugihara, K., Asano, M., and Narimatsu, H.
-
Journal Title
Scientific Reports
Volume: 2
Issue: 1
Pages: 680-680
DOI
Related Report
Peer Reviewed
-
[Journal Article] Essential role of endothelial class II PI3K-C2αin angiogenesis and vascular barrier function2012
Author(s)
Yoshioka K, Yoshida K, Cui H, Wakayama T, Takuwa N, Okamoto Y, Du W, Qi X, Asanuma K, Sugihara K, Aki S, Miyazawa H, Biswas K, Nagakura C, Ueno M, Iseki S, Schwartz JR, Okamoto H, Sasaki T,Matsui O, Asano M, Adams HR, Takakura N, Takuwa Y.
-
Journal Title
Nature med
Volume: 18
Issue: 10
Pages: 1560-1569
DOI
Related Report
Peer Reviewed
-
[Journal Article] Glycoprotein hyposialylation gives rise to a nephrotic-like syndrome that is prevented by sialic acid administration in GNE V572L point-mutant mice2012
Author(s)
Ito, M., Sugihara, K., Asaka, T., Toyama, T., Yo sh ih ar a, T., Furuichi, K., Wada, T. and Asano, M.
-
Journal Title
PLoS ONE
Volume: 7
Issue: 1
Pages: 1-13
DOI
Related Report
Peer Reviewed
-
-
-
-