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Molecular cloning and expression profiling of procollagen α1 (I) of cultured Pacific bluefin tuna

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Abstract

Type I collagen is widely distributed in most organs in teleosts. It plays a role not only in intercellular adhesion, but also in molecular signaling. In this study, Pacific bluefin tuna (PBT) procollagen α1 (I) cDNA was cloned and characterized. The nine fragments of a procollagen α1 (I) chain cDNA clone were prepared and spliced together to create the complete coding region. The resulting amino acid sequence was homologous with that of other teleosts. The mRNA expression profile of PBT procollagen α1 (I) in various tissues and the phylogenetic analysis with other vertebrate procollagen α1 (I) chains suggest that PBT procollagen α1 (I) could be a precursor form of the PBT type I collagen α1 chain. In addition, its level of expression in PBT larvae and early juveniles gradually increased with somatic growth. This increase was related to the standard length, wet body weight, and protein content of each individual fish. Therefore, the expression profile of procollagen α1 (I) may be a useful indicator for somatic growth in fish larvae and juveniles.

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Acknowledgments

The authors would like to thank the hatchery staff at the Fish Nursery Centers, Kinki University, for their tremendous technical support and advice. This work was supported by a grant-in-aid for the Global COE program “International education and research center for aquaculture science of bluefin tuna and other fish” from the Ministry of Education, Culture, Sports, Science and Technology of Japan. The gene expression analysis was supported by the JST/JICA SATREPS project on “Spawning ecology, nutritional requirement, and early life history of yellowfin tuna”.

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Correspondence to Yukio Kawamura.

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T. Tanaka and K. Takahashi contributed equally to this work.

The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession number AB824735.

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Tanaka, T., Takahashi, K., Adachi, K. et al. Molecular cloning and expression profiling of procollagen α1 (I) of cultured Pacific bluefin tuna. Fish Sci 80, 603–612 (2014). https://doi.org/10.1007/s12562-014-0737-7

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  • DOI: https://doi.org/10.1007/s12562-014-0737-7

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