Function analysis of Notch signaling system in spermatogenesis using gene transfer technique and its application for treatment of male infertility
Project/Area Number |
25861444
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Urology
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Research Institution | Nagoya City University |
Principal Investigator |
Iwatsuki Shoichiro 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (70595397)
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Research Collaborator |
KUBOTA Hiroki 名古屋市立大学, 大学院医学研究科, 研究員 (10347403)
UMEMOTO Ukihiro 名古屋市立大学, 大学院医学研究科, 講師 (80381812)
SASAKI Shoichi 名古屋市立大学, 大学院医学研究科, 准教授 (50225869)
KOHRI Kenjiro 名古屋市立大学, 学長 (30122047)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 精子形成 / 遺伝子導入 / Notchシグナル / 機能解析 / siRNA / Numb / Numb-like / 精細胞 |
Outline of Final Research Achievements |
In this study, we assessed functions of Numb and Numb-like, which are inhibitory factors of Notch signal pathway, in rat spermatogenesis using in vivo gene transfer technique. By immunohistochemistry, Numb and Numb-like were expressed in spermatocytes and later germ cells; however, Numb expression level was relatively lower in elongated spermatids then in spermatocytes and round spermatids. We constructed vector plasmid expressing siRNAs against Numb or Numb-like, which were transfected to rat testis in vivo. After knocking down of Numb or Numb-like in rat testis resulted in disturbance of differentiation of spermatocytes to spermatids. Our results suggested that Numb and Numb-like regulates differentiation of spermatids by inhibiting Notch signal pathway; Furthermore, it is suggested that Numb and Numb-like acts antagonistically each other.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Laparoscopic versus open radical cystectomy for patients older than 75 years: a single-center comparative analysis.2015
Author(s)
Yasui Takahiro, Tozawa Keiichi, Ando Ryosuke, Hamakawa Takashi, Iwatsuki Shoichiro, Taguchi Kazumi, Kobayashi Daichi, Naiki Taku, Mizuno Kentaro, Okada Atsushi, Umemoto Yukihiro, Kawai Noriyasu, Sasaki Shoichi, Hayashi Yutaro, Kohri Kenjiro
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Journal Title
Asian Pacific Journal of Cancer Prevention
Volume: 16
Issue: 15
Pages: 6353-6358
DOI
Related Report
Peer Reviewed
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