Budget Amount *help |
¥199,550,000 (Direct Cost: ¥153,500,000、Indirect Cost: ¥46,050,000)
Fiscal Year 2018: ¥48,620,000 (Direct Cost: ¥37,400,000、Indirect Cost: ¥11,220,000)
Fiscal Year 2017: ¥49,010,000 (Direct Cost: ¥37,700,000、Indirect Cost: ¥11,310,000)
Fiscal Year 2016: ¥51,350,000 (Direct Cost: ¥39,500,000、Indirect Cost: ¥11,850,000)
Fiscal Year 2015: ¥50,570,000 (Direct Cost: ¥38,900,000、Indirect Cost: ¥11,670,000)
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Outline of Final Research Achievements |
By intracellular total RNA sequencing for detection of RNA editing by AID, several C to U editing candidates were obtained. Motif analysis of hnRNP K, revealed GXXG and RGG motifs involved in antibody gene diversification which is useful for identifying C to U editing candidate. We showed that the 3 'UTR of topoisomerase 1 (Top 1) mRNA mediates the repression of Top 1 translation by AID. In addition to FACT complex and H3K4, SMARCA4 was newly discovered as Top1 binding protein. We revealed that SMARCA4 is required for recruitment of Top1 to the antibody gene, and FACT functions as an adapter between Top1 and H3K4me3. While searching for factors that specifically accumulate in the S region, we analyzed the dNTPase, SAMHD1, and found for the first time the role that the intracellular dNTP pool plays in the DNA repair process of CSR. We also found that the splicing factor Phf5a is also a factor necessary for the DNA repair process.
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