cis9, trans11-Conjugated Linoleic Acid Differentiates Mouse 3T3-L1 Preadipocytes into Mature Small Adipocytes through Induction of Peroxisome Proliferator-activated Receptor .GAMMA.
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- Sakuma Satoru
- Laboratory of Physiological Chemistry, Osaka University of Pharmaceutical Sciences
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- Nishioka Yuki
- Laboratory of Physiological Chemistry, Osaka University of Pharmaceutical Sciences
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- Imanishi Ryohta
- Laboratory of Physiological Chemistry, Osaka University of Pharmaceutical Sciences
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- Nishikawa Kenji
- Laboratory of Physiological Chemistry, Osaka University of Pharmaceutical Sciences
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- Sakamoto Hirotada
- Laboratory of Physiological Chemistry, Osaka University of Pharmaceutical Sciences
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- Fujisawa Junji
- Laboratory of Physiological Chemistry, Osaka University of Pharmaceutical Sciences
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- Wada Koichiro
- Department of Pharmacology, Graduate School of Dentistry, Osaka University
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- Kamisaki Yoshinori
- Department of Pharmacology, Graduate School of Dentistry, Osaka University
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- Fujimoto Yohko
- Laboratory of Physiological Chemistry, Osaka University of Pharmaceutical Sciences
Bibliographic Information
- Other Title
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- cis9, trans11-Conjugated Linoleic Acid Differentiates Mouse 3T3-L1 Preadipocytes into Mature Small Adipocytes through Induction of Peroxisome Proliferator-activated Receptor γ
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Abstract
Dietary conjugated linoleic acid (CLA) has been reported to exhibit a number of therapeutic effects in animal models and patients, such as anti-hypertensive, anti-hyperlipidemic, anti-arteriosclerotic, anti-carcinogenic, and anti-diabetic effects. However, the underlying mechanism is not well-characterized. In the present study, the effects of cis(c)9, trans(t)11-CLA on the differentiation of mouse 3T3-L1 preadipocytes into mature adipocytes were examined. Treatment with c9, t11-CLA in the presence of insulin, dexamethasone, and 3-isobutyl-1-methyl-xanthine (differentiation cocktail) significantly stimulated the accumulation of triacylglycerol. The microscopic observation of cells stained by Oil Red O demonstrated that c9, t11-CLA increases the amount and proportion of small mature adipocytes secreting adiponectin, a benign adipocytokine, when compared to the differentiation cocktail alone. Furthermore, c9, t11-CLA increased bioactive peroxisome proliferator-activated receptor γ (PPARγ) levels in a nuclear extract of 3T3-L1 cells, suggesting the enhancing effect of this fatty acid on the nuclear transmission of PPARγ, a master regulator of adipocyte differentiation, in 3T3-L1 cells. These results suggest that the therapeutic effects of c9, t11-CLA on lifestyle-related diseases are partially due to the enhanced formation of small adipocytes from preadipocytes via PPARγ stimulation.<br>
Journal
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- Journal of Clinical Biochemistry and Nutrition
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Journal of Clinical Biochemistry and Nutrition 47 (2), 167-173, 2010
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Details
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- CRID
- 1390001204671170560
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- NII Article ID
- 130004879208
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- ISSN
- 18805086
- 09120009
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed