Oocyte-specific spindle scaling
Project/Area Number |
26650072
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Cell biology
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Kitajima Tomoya 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, チームリーダー (00376641)
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Co-Investigator(Renkei-kenkyūsha) |
Kyogoku Hirohisa 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, 訪問研究員 (20726038)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 卵母細胞 / 紡錘体 / 染色体分配 / 減数分裂 |
Outline of Final Research Achievements |
This study aimed to reveal the mechanisms and roles of spindle scaling in oocytes. Four-dimensional live analysis of mouse oocytes following artificial cytoplasmic increase or decrease demonstrated that spindle volume is influenced by cytoplasmic volume. Further investigation of the functionality of the spindles indicated that a larger cytoplasm lowers the spindle functionality and increases the frequency of chromosome segregation errors. These results suggested that the large cytoplasm of oocytes is a cell-intrinsic feature that predisposes to chromosome segregation errors (Kyogoku & Kitajima 2017 Dev Cell).
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Meikin is a conserved regulator of meiosis-I-specific kinetochore function2015
Author(s)
Kim, J., Ishiguro, K., Nambu, A., Akiyoshi, B., Yokobayashi, S., Kagami, A., Ishiguro, T., Pendas, A.M., Takeda, N., Sakakibara, Y., Kitajima, T.S., Tanno, Y., Sakuno, T., and Watanabe, Y.
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Journal Title
Nature
Volume: 517
Issue: 7535
Pages: 466-471
DOI
Related Report
Peer Reviewed
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