KOSAKA Megumi 小坂恵

… Alternative Names

小坂惠コサカメグミ

小坂恵

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Researcher Number	00170233
Other IDs
Affiliation (based on the past Project Information) *help	2005 – 2010: 岡山大学, 自然生命科学研究支援センター, 助手 2006: 岡山大学, 自然生命科学支援センター, 助手 1994 – 1995: 岡山大学, 工学部, 教務員 1989 – 1991: 岡山大学, 工学部, 教務員 1990: 岡山大学, 工学部, 教務職員 1986 – 1987: 岡山大学, 工学部, 教務員
Review Section/Research Field	Except Principal Investigator Biofunction/Bioprocess / 有機工業化学 / 計測・制御工学
Keywords	Except Principal Investigator タンパク質工学 / 長光路 / protein engineering / フォルディング / 細胞内導入 / タンパク質 / 化学修飾 / バイオテクノロジー / 実時間 / 鉛塩半導体レ-ザ … More / 半導体レ-ザ / 水田 / メタン / biotechnology / cell culture / protein purfication / folding / protein transduction / chemical modification / Protein / 培養細胞 / 分離・精製 / inclusion body / solubility of denatured protein / thiol-protecting reagent / 可逆的チオール修飾試薬 / 変性タンパク質の可溶化 / インクルージョンボディ / 変性タンパク質の溶解度 / チオール保護試薬 / Tunable-Diode-Laser / Scintillation / Long-path / Non-contact / Real-Time / Concentration / Methane / Atmosphere / シンチレ-ション / 非接触 / 濃度 / メタンガス / 大気 / 波形観測Cuドープ / 導波路光ゲート / ピコ秒レーザパルス / LiTaO_3 / 波形観測 / レーザパルス / 熱拡散 / 光導波路 / タンタル酸リチウム / 光IC / バィオテクナロジー / フオルデイング / 格子工学 / 可溶化 / 結晶化 / 蛋白質 / 生物・生体工学 / 開放大気 / 戻り光 Less

Development of analytical and production method for problematic protein by artificial control of physical property of protein
- Principal Investigator
  
  YAMADA Hidenori
- Project Period (FY)
  2007 – 2010
- Research Category
  
  Grant-in-Aid for Scientific Research (A)
- Research Field
  
  Biofunction/Bioprocess
- Research Institution
  Okayama University
Development and application of in cell folding technology based on reversible cationization of denatured proteins.
- Principal Investigator
  
  YAMADA Hidenori
- Project Period (FY)
  2005 – 2006
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Biofunction/Bioprocess
- Research Institution
  OKYAMA UNIVERSITY
Preparation and application to protein engineering of thiol-protecting reagent having charges.
- Principal Investigator
  
  YAMADA Hidenori
- Project Period (FY)
  1994 – 1995
- Research Category
  
  Grant-in-Aid for General Scientific Research (B)
- Research Field
  
  有機工業化学
- Research Institution
  Okayama University
水田における大気中メタンの野外・実時間計測
- Principal Investigator
  
  古賀隆治
- Project Period (FY)
  1991
- Research Category
  
  Grant-in-Aid for Scientific Research on Priority Areas
- Research Institution
  Okayama University
水田における大気中メタンの野外・実時間計測
- Principal Investigator
  
  KOGA Ryuji
- Project Period (FY)
  1990
- Research Category
  
  Grant-in-Aid for Scientific Research on Priority Areas
- Research Institution
  Okayama University
Development of a Device for Trace Gas Concentration Measurement Based on the Quick Scanning Tunable Diode Absorption Spectrometry
- Principal Investigator
  
  KOGA Ryuji
- Project Period (FY)
  1989 – 1991
- Research Category
  
  Grant-in-Aid for Developmental Scientific Research
- Research Field
  
  計測・制御工学
- Research Institution
  Okayama University.
導波路型光ゲートを用いる超高速光信号処理
- Principal Investigator
  
  古賀隆治
- Project Period (FY)
  1987
- Research Category
  
  Grant-in-Aid for Special Project Research
- Research Institution
  Okayama University
導波路型光ゲートを用いる超高速光信号処理
- Principal Investigator
  
  古賀隆治
- Project Period (FY)
  1986
- Research Category
  
  Grant-in-Aid for Special Project Research
- Research Institution
  Okayama University

All 2008 2007 2006 2005

All Journal Article Presentation

[Journal Article] Transient cell proliferation with polyethylenimine-cationized N-terminal domain of Simian Virus 40 large T-antigen2008
- Author(s)
  Murata H., Futami J., Kitazoe M., Kosaka M., Tada H., Seno M., Yamada H.
- Journal Title
  
  J.Biosci.Bioeng 105
  
  Pages: 34-38
- NAID
  110006570773
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19206085
[Journal Article] Intracellular delivery of glutathione S-transferase-fused proteins into mammalian cells by polyethylenimine-glutathione conjugates.2008
- Author(s)
  Hitoshi Muratal, Junichiro Futami, Midori Kitazoe, Takayuki Yonehara, Hidetaka Nakanishi, Megumi Kosaka, Hiroko Tada, Masakiyo Sakaguchi, Yasuyuki Yagi, Masaharu Seno, Nam-ho Huh, Hidenori Yamada.
- Journal Title
  
  Journal of Biochemistry 144(4)
  
  Pages: 447-455
- NAID
  10024912690
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19206085
[Journal Article] Transient cell proliferation with polyethylenimine-cationized N-terminal domain of Simian Virus 40 large T-antigen.2008
- Author(s)
  Murata H., Futami J., Kitazoe M., Kosaka M., Tada H., Seno M., Yamada H.
- Journal Title
  
  J.Biosci.Bioeng. 105
  
  Pages: 34-38
- NAID
  110006570773
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19206085
[Journal Article] Intracellular delivery of glutathione S-transferase-fused proteins into mammalian cells by polyethylenimine-glutathione conjugates2008
- Author(s)
  Hitoshi Murata, Junichiro Futami, Midori Kitazoe, Takayuki Yonehara, Hidetaka Nakanishi, Megumi Kosaka, Hiroko Tada, Masakiyo Sakaguchi, Yasuyuki Yagi, Masaharu Seno, Nam-ho Huh, Hidenori Yamada
- Journal Title
  
  Journal of Biochemistry 144(4)
  
  Pages: 447-455
- NAID
  10024912690
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19206085
[Journal Article] 'Crystal lattice engineering,' an approach to engineer protein crystal contacts by creating intermolecular symmetry : crystallization and structure determination of a mutant human RNase 1 with a hydrophobic interface of leucines.2007
- Author(s)
  Yamada H., Tamada T., Kosaka M., Miyata K., Fujiki S., Tano M., Moriya M., Yamanishi M., Honjo E., Tada H., Ino T., Yamaguchi H., Futami J., Seno M., Nomoto T., Hirata T., Yoshimura M., Kuroki R.
- Journal Title
  
  Protein Sci. 16
  
  Pages: 1389-1397
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19206085
[Journal Article] Denatured and reversibly cationized p53 readily enters cells and simultaneously folds to the functional protein in the cells.2006
- Author(s)
  Murata H., Sakaguchi M., Futami J., Kitazoe M., Maeda T., Doura H., Kosaka M.Tada H., Seno M., Huh N.H., Yamada H.
- Journal Title
  
  Biochemistry 45
  
  Pages: 6124-6132
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-17360399
[Journal Article] Intracellular delivery of proteins into mammalian living cells by polyethylenimine-cationization.2005
- Author(s)
  Futami J., Kitazoe M., Maeda T., Nukui E., Sakaguchi M., Kosaka J., Miyazaki M., Kosaka M., Tada H., Seno M., Sasaki J., Huh N.-H., Namba M., Yamada H.
- Journal Title
  
  J. Biosci. Bioeng. 99
  
  Pages: 95-103
- NAID
  110002695633
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-17360399
[Journal Article] Protein transduction assisted by polyethylenimine-cationized carrier proteins.2005
- Author(s)
  Kitazoe M., Murata H., Futami J., Maeda T., Sakaguchi M., Miyazaki M., Kosaka M., Tada H., Seno M., Huh N.H., Namba M., Nishikawa M., Maeda Y., Yamada H.
- Journal Title
  
  J. Biochem. (Tokyo) 137
  
  Pages: 693-701
- NAID
  10017345558
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-17360399
[Presentation] 結晶格子工学によるウシRNase Aの結晶空間群の変更2008
- Author(s)
  土井慎一, 小坂恵, 二見淳一郎, 多田宏子, 玉田太郎, 岡崎伸生, 黒木良太, 山田秀徳
- Organizer
  第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
- Year and Date
  2008-12-12
- Data Source
  KAKENHI-PROJECT-19206085
[Presentation] 変性タンパク質の可溶化と細胞内導入を目的とした新規SH基多価カチオン化試薬の合成と解析2008
- Author(s)
  穴井祐介, 矢木恵一郎, 二見淳一郎, 小坂恵, 多田宏子, 山田秀徳
- Organizer
  第31回日本分子生物学会年会・第81回日本生化学会大会合同大会 (BMB2008)
- Year and Date
  2008-12-12
- Data Source
  KAKENHI-PROJECT-19206085
[Presentation] 変性タンパク質の可溶化と細胞内導入を目的とした新規SH基多価カチオン化試薬の合成と解析2008
- Author(s)
  穴井祐介, 矢木恵一郎, 二見淳一郎, 小坂恵, 多田宏子, 山田秀徳
- Organizer
  第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
- Year and Date
  2008-12-12
- Data Source
  KAKENHI-PROJECT-19206085
[Presentation] (*原研) 結晶格子工学によるウシRNase Aの結晶空間群の変更2008
- Author(s)
  土井慎一, 小坂恵, 二見淳一郎, 多田宏子, 玉田太郎, 岡崎伸生, 黒木良太, 山田秀徳
- Organizer
  第31回日本分子生物学会年会・第81回日本生化学会大会合同大会 (BMB2008)
- Year and Date
  2008-12-12
- Data Source
  KAKENHI-PROJECT-19206085
[Presentation] 人工転写因子を用いたタンパク質細胞内導入効率の定量化2007
- Author(s)
  二見淳一郎、浅間孝志、北添翠、村田等、小坂恵、多田宏子、妹尾昌治、山田秀徳
- Organizer
  第30回日本分子生物学会年会・第80回日本生化学会大会合同大会(BMB2007)
- Year and Date
  2007-12-12
- Data Source
  KAKENHI-PROJECT-19206085
[Presentation] ポリエチレンイミン(PEI)グルタチオンキャリアーを用いたGST-融合タンパク質の細胞導入2007
- Author(s)
  村田等、二見淳一郎、北添翠、小坂恵、多田宏子、甲斐敬、妹尾昌治、山田秀徳
- Organizer
  第59回日本生物工学会大会
- Year and Date
  2007-09-26
- Data Source
  KAKENHI-PROJECT-19206085

1. KOGA Ryuji (20027147)

# of Collaborated Projects: 5 results

# of Collaborated Products: 0 results
2. SANO Hiroya (10033143)

# of Collaborated Projects: 5 results

# of Collaborated Products: 0 results
3. SENOO Masaharu (90243493)

# of Collaborated Projects: 3 results

# of Collaborated Products: 10 results
4. YAMADA Hidenori (80037613)

# of Collaborated Projects: 3 results

# of Collaborated Products: 14 results
5. FUTAMI Jyunichirou (00420498)

# of Collaborated Projects: 2 results

# of Collaborated Products: 14 results
6. WADA Osami (10210973)

# of Collaborated Projects: 2 results

# of Collaborated Products: 0 results
7. TADA Hiroko (60271061)

# of Collaborated Projects: 2 results

# of Collaborated Products: 14 results

KOSAKA Megumi 小坂 恵

小坂 惠 コサカ メグミ

小坂 恵

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[Journal Article] Transient cell proliferation with polyethylenimine-cationized N-terminal domain of Simian Virus 40 large T-antigen2008

Author(s)

Journal Title

NAID

Data Source

[Journal Article] Intracellular delivery of glutathione S-transferase-fused proteins into mammalian cells by polyethylenimine-glutathione conjugates.2008

Author(s)

Journal Title

NAID

Data Source

[Journal Article] Transient cell proliferation with polyethylenimine-cationized N-terminal domain of Simian Virus 40 large T-antigen.2008

Author(s)

Journal Title

NAID

Data Source

[Journal Article] Intracellular delivery of glutathione S-transferase-fused proteins into mammalian cells by polyethylenimine-glutathione conjugates2008

Author(s)

Journal Title

NAID

Data Source

[Journal Article] 'Crystal lattice engineering,' an approach to engineer protein crystal contacts by creating intermolecular symmetry : crystallization and structure determination of a mutant human RNase 1 with a hydrophobic interface of leucines.2007

Author(s)

Journal Title

Data Source

[Journal Article] Denatured and reversibly cationized p53 readily enters cells and simultaneously folds to the functional protein in the cells.2006

Author(s)

Journal Title

Description

Data Source

[Journal Article] Intracellular delivery of proteins into mammalian living cells by polyethylenimine-cationization.2005

Author(s)

Journal Title

NAID

Description

Data Source

[Journal Article] Protein transduction assisted by polyethylenimine-cationized carrier proteins.2005

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Journal Title

KOSAKA Megumi 小坂恵

小坂惠コサカメグミ

小坂恵