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Nishida Keiji  西田 敬二

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NISHIDA KEIJI  西田 敬二

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Researcher Number 10620338
Other IDs
Affiliation (Current) 2025: 神戸大学, 先端バイオ工学研究センター, 教授
Affiliation (based on the past Project Information) *help 2018 – 2020: 神戸大学, 先端バイオ工学研究センター, 教授
2016 – 2017: 神戸大学, 科学技術イノベーション研究科, 教授
2015: 神戸大学, 学内共同利用施設等, 准教授
2014: 神戸大学, 自然科学系先端融合研究環重点研究部, 准教授
Review Section/Research Field
Principal Investigator
System genome science / Complex systems
Keywords
Principal Investigator
合成生物 / CRISPR / Target-AID / ゲノム編集 / 合成生物学 / synthetic biology / 遺伝子 / ゲノム / バイオテクノロジー / Base editing … More / 塩基編集 / グリコシラーゼ / Cas9 / Target-G / Targegt-AID / 人工進化 / 進化 / 合成進化 / デアミナーゼ Less
  • Research Projects

    (3 results)
  • Research Products

    (29 results)
  • Co-Researchers

    (6 People)
  •  Creation of artificial enzymes that enable targeted editing of any DNA basesPrincipal Investigator

    • Principal Investigator
      Nishida Keiji
    • Project Period (FY)
      2017 – 2020
    • Research Category
      Grant-in-Aid for Young Scientists (A)
    • Research Field
      System genome science
    • Research Institution
      Kobe University
  •  Creation of self-evolving enzymatic system that enables semi rational design of genomePrincipal Investigator

    • Principal Investigator
      NISHIDA KEIJI
    • Project Period (FY)
      2016 – 2017
    • Research Category
      Grant-in-Aid for Challenging Exploratory Research
    • Research Field
      System genome science
    • Research Institution
      Kobe University
  •  DNA 塩基置換酵素の“逆進化”誘導によるDNAを切らないゲノム編集ツールの開発Principal Investigator

    • Principal Investigator
      西田 敬二
    • Project Period (FY)
      2014 – 2015
    • Research Category
      Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
    • Review Section
      Complex systems
    • Research Institution
      Kobe University

All 2021 2020 2019 2018 2017 2016 2015 2014

All Journal Article Presentation Book Patent

  • [Book] 完全版 ゲノム編集実験スタンダード2019

    • Author(s)
      中井明日也,Ang Li,西田敬二
    • Total Pages
      386
    • Publisher
      羊土社
    • ISBN
      9784758122443
    • Data Source
      KAKENHI-PROJECT-17H04994
  • [Journal Article] CRISPR-derived genome editing technologies for metabolic engineering2021

    • Author(s)
      Nishida Keiji、Kondo Akihiko
    • Journal Title

      Metabolic Engineering

      Volume: 63 Pages: 141-147

    • DOI

      10.1016/j.ymben.2020.12.002

    • Data Source
      KAKENHI-PROJECT-17H04994
  • [Journal Article] Efficient base editing in tomato using a highly expressed transient system2021

    • Author(s)
      Yuan Shaoze、Kawasaki Shunsuke、Abdellatif Islam M. Y.、Nishida Keiji、Kondo Akihiko、Ariizumi Tohru、Ezura Hiroshi、Miura Kenji
    • Journal Title

      Plant Cell Reports

      Volume: 40 Issue: 4 Pages: 667-676

    • DOI

      10.1007/s00299-021-02662-z

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-17H04994, KAKENHI-PROJECT-20K21302
  • [Journal Article] Base editors for simultaneous introduction of C-to-T and A-to-G mutations2020

    • Author(s)
      Sakata Rina C.、Ishiguro Soh、Mori Hideto、Tanaka Mamoru、Tatsuno Kenji、Ueda Hiroki、Yamamoto Shogo、Seki Motoaki、Masuyama Nanami、Nishida Keiji、Nishimasu Hiroshi、Arakawa Kazuharu、Kondo Akihiko、Nureki Osamu、Tomita Masaru、Aburatani Hiroyuki、Yachie Nozomu
    • Journal Title

      Nature Biotechnology

      Volume: na Issue: 7 Pages: 865-869

    • DOI

      10.1038/s41587-020-0509-0

    • Peer Reviewed / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-18H02428, KAKENHI-PROJECT-18K19777, KAKENHI-PROJECT-17K07195, KAKENHI-PROJECT-17H04994
  • [Journal Article] Fruit setting rewires central metabolism via gibberellin cascades2020

    • Author(s)
      Shinozaki Yoshihito et al.
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 117 Issue: 38 Pages: 23970-23981

    • DOI

      10.1073/pnas.2011859117

    • Peer Reviewed / Open Access / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-20K06043, KAKENHI-PROJECT-19K23672, KAKENHI-PROJECT-16H06279, KAKENHI-PROJECT-17H04994
  • [Journal Article] Multiple gene substitution by Target-AID base-editing technology in tomato2020

    • Author(s)
      Hunziker Johan、Nishida Keiji、Kondo Akihiko、Kishimoto Sanae、Ariizumi Tohru、Ezura Hiroshi
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 20471-20471

    • DOI

      10.1038/s41598-020-77379-2

    • NAID

      120007028992

    • Data Source
      KAKENHI-PROJECT-17H04994
  • [Journal Article] Production of Herbicide-Sensitive Strain to Prevent Volunteer Rice Infestation Using a CRISPR-Cas9 Cytidine Deaminase Fusion2020

    • Author(s)
      Komatsu Akira、Ohtake Miki、Shimatani Zenpei、Nishida Keiji
    • Journal Title

      Frontiers in Plant Science

      Volume: 11 Pages: 00925-00925

    • DOI

      10.3389/fpls.2020.00925

    • Data Source
      KAKENHI-PROJECT-17H04994
  • [Journal Article] Targeted Base Editing with CRISPR-Deaminase in Tomato2019

    • Author(s)
      Shimatani Zenpei、Ariizumi Tohru、Fujikura Ushio、Kondo Akihiko、Ezura Hiroshi、Nishida Keiji
    • Journal Title

      Methods Mol Biol.

      Volume: 1917 Pages: 297-307

    • DOI

      10.1007/978-1-4939-8991-1_22

    • ISBN
      9781493989904, 9781493989911
    • Data Source
      KAKENHI-PROJECT-17H04994
  • [Journal Article] Mammalian synthetic biology by CRISPRs engineering and applications2019

    • Author(s)
      Katayama Kenta、Mitsunobu Hitoshi、Nishida Keiji
    • Journal Title

      Current Opinion in Chemical Biology

      Volume: 52 Pages: 79-84

    • DOI

      10.1016/j.cbpa.2019.05.020

    • Data Source
      KAKENHI-PROJECT-17H04994
  • [Journal Article] A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall2019

    • Author(s)
      Furuhata Yuichi、Sakai Ayako、Murakami Tomi、Morikawa Mone、Nakamura Chikashi、Yoshizumi Takeshi、Fujikura Ushio、Nishida Keiji、Kato Yoshio
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 2163-2163

    • DOI

      10.1038/s41598-018-38119-9

    • NAID

      120006559318

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-17H04994
  • [Journal Article] In vivo targeted single-nucleotide editing in zebrafish.2018

    • Author(s)
      Tanaka, S., Yoshioka, S., Nishida, K., Hosokawa, H., Kakizuka, A., and Maegawa, S.
    • Journal Title

      Sci. Rep.

      Volume: 8 Issue: 1 Pages: 11423-11423

    • DOI

      10.1038/s41598-018-29794-9

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-18J14073, KAKENHI-PROJECT-16H05151, KAKENHI-PROJECT-17H04994
  • [Journal Article] Deaminase-mediated multiplex genome editing in Escherichia coli2018

    • Author(s)
      Banno S, Nishida K, Arazoe T, Mitsunobu H and Kondo A.
    • Journal Title

      Nature Microbiology

      Volume: 3 Issue: 4 Pages: 423-429

    • DOI

      10.1038/s41564-017-0102-6

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-17K17879, KAKENHI-PROJECT-17H05021, KAKENHI-PROJECT-16K14654, KAKENHI-PROJECT-17H04994
  • [Journal Article] Genetic and physiological basis for antibody production by Kluyveromyces marxianus2018

    • Author(s)
      Nambu-Nishida Yumiko、Nishida Keiji、Hasunuma Tomohisa、Kondo Akihiko
    • Journal Title

      AMB Express

      Volume: 8 Issue: 1 Pages: 56-56

    • DOI

      10.1186/s13568-018-0588-1

    • NAID

      120006462314

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-16K14654, KAKENHI-PROJECT-17H04994
  • [Journal Article] Inheritance of co-edited genes by CRISPR-based targeted nucleotide substitutions in rice2018

    • Author(s)
      Shimatani Zenpei、Fujikura Ushio、Ishii Hisaki、Matsui Yusuke、Suzuki Minoru、Ueke Yuki、Taoka Ken-ichiro、Terada Rie、Nishida Keiji、Kondo Akihiko
    • Journal Title

      Plant Physiology and Biochemistry

      Volume: 131 Pages: 78-83

    • DOI

      10.1016/j.plaphy.2018.04.028

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-17H04994, KAKENHI-PROJECT-16K07560
  • [Journal Article] Herbicide tolerance-assisted multiplex targeted nucleotide substitution in rice2018

    • Author(s)
      Shimatani Zenpei、Fujikura Ushio、Ishii Hisaki、Terada Rie、Nishida Keiji、Kondo Akihiko
    • Journal Title

      Data in Brief

      Volume: 20 Pages: 1325-1331

    • DOI

      10.1016/j.dib.2018.08.124

    • NAID

      120006543325

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-17H04994
  • [Journal Article] Targeted nucleotide editing technologies for microbial metabolic engineering2018

    • Author(s)
      Arazoe, T., Kondo, A., Nishida, K.
    • Journal Title

      Biotechnology Journal

      Volume: 13 Issue: 9 Pages: 170596-170596

    • DOI

      10.1002/biot.201700596

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-18K19218, KAKENHI-PROJECT-17H05021, KAKENHI-PROJECT-17H04994
  • [Journal Article] Glycosyltransferase MDR1 assembles a dividing ring for mitochondrial proliferation comprising polyglucan nanofilaments2017

    • Author(s)
      Yoshida Yamato, Kuroiwa Haruko, Shimada Takashi, Yoshida Masaki, Ohnuma Mio, Fujiwara Takayuki, Imoto Yuuta, Yagisawa Fumi, Nishida Keiji, Hirooka Shunsuke, Misumi Osami, Mogi Yuko, Akakabe Yoshihiko, Matsushita Kazunobu, Kuroiwa Tsuneyoshi
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 114 Issue: 50 Pages: 13284-13289

    • DOI

      10.1073/pnas.1715008114

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-17K00676, KAKENHI-PROJECT-16H04813, KAKENHI-PROJECT-15K18588, KAKENHI-PROJECT-17H04994
  • [Journal Article] Beyond Native Cas9: Manipulating Genomic Information and Function2017

    • Author(s)
      Mitsunobu Hitoshi、Teramoto Jun、Nishida Keiji、Kondo Akihiko
    • Journal Title

      Trends in Biotechnology

      Volume: 35 Issue: 10 Pages: 983-996

    • DOI

      10.1016/j.tibtech.2017.06.004

    • Data Source
      KAKENHI-PROJECT-16K14654, KAKENHI-PROJECT-17H04994
  • [Journal Article] Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion2017

    • Author(s)
      Shimatani, Z., Kashojiya, S., Takayama, M., Terada, R., Arazoe, T., Ishii, H., Teramura, H., Yamamoto, T., Komatsu, H., Miura, K., Ezura, H., Nishida, K., Ariizumi, T. and Kondo A.
    • Journal Title

      Nature Biotechnology

      Volume: 印刷中 Issue: 5 Pages: 441-443

    • DOI

      10.1038/nbt.3833

    • Peer Reviewed / Acknowledgement Compliant
    • Data Source
      KAKENHI-PROJECT-15K18647, KAKENHI-PROJECT-16K07560, KAKENHI-PROJECT-16K14654, KAKENHI-PUBLICLY-16H01458
  • [Journal Article] Development of a comprehensive set of tools for genome engineering in a cold- and thermo-tolerant Kluyveromyces marxianus yeast strain2017

    • Author(s)
      Nambu-Nishida Yumiko、Nishida Keiji、Hasunuma Tomohisa、Kondo Akihiko
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 8993-8993

    • DOI

      10.1038/s41598-017-08356-5

    • NAID

      120006346142

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-16K14654, KAKENHI-PROJECT-17H04994
  • [Journal Article] Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems2016

    • Author(s)
      Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki, M., Hara, K.Y., Shimatani, Z. and Kondo, A.
    • Journal Title

      Science

      Volume: 353 Issue: 6305 Pages: 6305-6305

    • DOI

      10.1126/science.aaf8729

    • Peer Reviewed / Open Access / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-26430198, KAKENHI-PROJECT-15K18647, KAKENHI-PROJECT-16K14654, KAKENHI-PROJECT-15K18466, KAKENHI-PROJECT-15K14436
  • [Journal Article] Evaluation of genes involved in oxidative phosphorylation in yeast by developing a simple and rapid method to measure mitochondrial ATP synthetic activity.2015

    • Author(s)
      Ye X, Morikawa K, Ho SH, Araki M, Nishida K, Hasunuma T, Hara KY, Kondo A.
    • Journal Title

      Microbial Cell Factories

      Volume: 14 Issue: 1 Pages: 56-56

    • DOI

      10.1186/s12934-015-0239-z

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PUBLICLY-26119710, KAKENHI-PROJECT-26430198, KAKENHI-PUBLICLY-26119711, KAKENHI-PUBLICLY-26117715
  • [Journal Article] Improvement of oxidized glutathione fermentation by thiol redox metabolism engineering in Saccharomyces cerevisiae.2015

    • Author(s)
      Hara KY, Aoki N, Kobayashi J, Kiriyama K, Nishida K, Araki M, Kondo A.
    • Journal Title

      Appl Microbiol Biotechnol.

      Volume: Nov;99(22) Pages: 9771-8

    • Peer Reviewed
    • Data Source
      KAKENHI-PUBLICLY-26119710
  • [Patent] 標的化したDNA配列の核酸塩基を特異的に変換する、単子葉植物のゲノム配列の変換方法、及びそれに用いる分子複合体2015

    • Inventor(s)
      西田敬二、島谷善平、近藤昭彦
    • Industrial Property Rights Holder
      国立大学法人神戸大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-11-27
    • Data Source
      KAKENHI-PUBLICLY-26119710
  • [Patent] 標的化したDNA配列の核酸塩基を特異的に変換するゲノム配列の改変方法及びそれに用いる分子複合体2015

    • Inventor(s)
      西田敬二、近藤昭彦、坂野聡美
    • Industrial Property Rights Holder
      神戸大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-03-04
    • Overseas
    • Data Source
      KAKENHI-PUBLICLY-26119710
  • [Patent] 細胞内でDNAを切断せずに脱塩基反応によりゲノム中の        狙った領域に変異を導入する技術2014

    • Inventor(s)
      西田敬二、近藤昭彦
    • Industrial Property Rights Holder
      神戸大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-224745
    • Filing Date
      2014-11-04
    • Data Source
      KAKENHI-PUBLICLY-26119710
  • [Presentation] ゲノムを切らずに書き換える新たなゲノム編集技術の開発2015

    • Author(s)
      西田 敬二, 坂野 聡美, 近藤 昭彦
    • Organizer
      第67回日本生物工学会 大会
    • Place of Presentation
      鹿児島県鹿児島市
    • Year and Date
      2015-10-26
    • Data Source
      KAKENHI-PUBLICLY-26119710
  • [Presentation] Targeted base exchange-mediated genome editing2015

    • Author(s)
      西田 敬二, 坂野 聡美, 荒添貴之、島谷善平、近藤 昭彦
    • Organizer
      BMB2015
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2015-12-03
    • Int'l Joint Research
    • Data Source
      KAKENHI-PUBLICLY-26119710
  • [Presentation] Pin-point genome editing without cleaving DNA2015

    • Author(s)
      西田 敬二
    • Organizer
      植物ゲノム編集技術に関する国際シンポジウム
    • Place of Presentation
      茨城県つくば市
    • Year and Date
      2015-11-28
    • Invited / Int'l Joint Research
    • Data Source
      KAKENHI-PUBLICLY-26119710
  • 1.  BANNO Satomi
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 1 results
  • 2.  MISUMI Osami
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 1 results
  • 3.  ARAKI Michihiro
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 1 results
  • 4.  大沼 みお
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 1 results
  • 5.  谷内江 望
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 1 results
  • 6.  荒添 貴之
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 2 results

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