Nishida Keiji 西田敬二

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NISHIDA KEIJI 西田敬二

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Researcher Number	10620338
Other IDs
Affiliation (Current)	2025: 神戸大学, 先端バイオ工学研究センター, 教授
Affiliation (based on the past Project Information) *help	2018 – 2020: 神戸大学, 先端バイオ工学研究センター, 教授 2016 – 2017: 神戸大学, 科学技術イノベーション研究科, 教授 2015: 神戸大学, 学内共同利用施設等, 准教授 2014: 神戸大学, 自然科学系先端融合研究環重点研究部, 准教授
Review Section/Research Field	Principal Investigator System genome science / Complex systems
Keywords	Principal Investigator 合成生物 / CRISPR / Target-AID / ゲノム編集 / 合成生物学 / synthetic biology / 遺伝子 / ゲノム / バイオテクノロジー / Base editing … More / 塩基編集 / グリコシラーゼ / Cas9 / Target-G / Targegt-AID / 人工進化 / 進化 / 合成進化 / デアミナーゼ Less

Creation of artificial enzymes that enable targeted editing of any DNA basesPrincipal Investigator
- Principal Investigator
  
  Nishida Keiji
- Project Period (FY)
  2017 – 2020
- Research Category
  
  Grant-in-Aid for Young Scientists (A)
- Research Field
  
  System genome science
- Research Institution
  Kobe University
Creation of self-evolving enzymatic system that enables semi rational design of genomePrincipal Investigator
- Principal Investigator
  
  NISHIDA KEIJI
- Project Period (FY)
  2016 – 2017
- Research Category
  
  Grant-in-Aid for Challenging Exploratory Research
- Research Field
  
  System genome science
- Research Institution
  Kobe University
DNA 塩基置換酵素の“逆進化”誘導によるDNAを切らないゲノム編集ツールの開発Principal Investigator
- Principal Investigator
  
  西田敬二
- Project Period (FY)
  2014 – 2015
- Research Category
  
  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
- Review Section
  
  Complex systems
- Research Institution
  Kobe University

All 2021 2020 2019 2018 2017 2016 2015 2014

All Journal Article Presentation Book Patent

[Book] 完全版　ゲノム編集実験スタンダード2019
- Author(s)
  中井明日也，Ang Li，西田敬二
- Total Pages
  386
- Publisher
  羊土社
- ISBN
  9784758122443
- Data Source
  KAKENHI-PROJECT-17H04994
[Journal Article] CRISPR-derived genome editing technologies for metabolic engineering2021
- Author(s)
  Nishida Keiji、Kondo Akihiko
- Journal Title
  
  Metabolic Engineering
  
  Volume: 63 Pages: 141-147
- DOI
  10.1016/j.ymben.2020.12.002
- Data Source
  KAKENHI-PROJECT-17H04994
[Journal Article] Efficient base editing in tomato using a highly expressed transient system2021
- Author(s)
  Yuan Shaoze、Kawasaki Shunsuke、Abdellatif Islam M. Y.、Nishida Keiji、Kondo Akihiko、Ariizumi Tohru、Ezura Hiroshi、Miura Kenji
- Journal Title
  
  Plant Cell Reports
  
  Volume: 40 Issue: 4 Pages: 667-676
- DOI
  10.1007/s00299-021-02662-z
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17H04994, KAKENHI-PROJECT-20K21302
[Journal Article] Base editors for simultaneous introduction of C-to-T and A-to-G mutations2020
- Author(s)
  Sakata Rina C.、Ishiguro Soh、Mori Hideto、Tanaka Mamoru、Tatsuno Kenji、Ueda Hiroki、Yamamoto Shogo、Seki Motoaki、Masuyama Nanami、Nishida Keiji、Nishimasu Hiroshi、Arakawa Kazuharu、Kondo Akihiko、Nureki Osamu、Tomita Masaru、Aburatani Hiroyuki、Yachie Nozomu
- Journal Title
  
  Nature Biotechnology
  
  Volume: na Issue: 7 Pages: 865-869
- DOI
  10.1038/s41587-020-0509-0
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18H02428, KAKENHI-PROJECT-18K19777, KAKENHI-PROJECT-17K07195, KAKENHI-PROJECT-17H04994
[Journal Article] Fruit setting rewires central metabolism via gibberellin cascades2020
- Author(s)
  Shinozaki Yoshihito et al.
- Journal Title
  
  Proceedings of the National Academy of Sciences
  
  Volume: 117 Issue: 38 Pages: 23970-23981
- DOI
  10.1073/pnas.2011859117
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-20K06043, KAKENHI-PROJECT-19K23672, KAKENHI-PROJECT-16H06279, KAKENHI-PROJECT-17H04994
[Journal Article] Multiple gene substitution by Target-AID base-editing technology in tomato2020
- Author(s)
  Hunziker Johan、Nishida Keiji、Kondo Akihiko、Kishimoto Sanae、Ariizumi Tohru、Ezura Hiroshi
- Journal Title
  
  Scientific Reports
  
  Volume: 10 Issue: 1 Pages: 20471-20471
- DOI
  10.1038/s41598-020-77379-2
- NAID
  120007028992
- Data Source
  KAKENHI-PROJECT-17H04994
[Journal Article] Production of Herbicide-Sensitive Strain to Prevent Volunteer Rice Infestation Using a CRISPR-Cas9 Cytidine Deaminase Fusion2020
- Author(s)
  Komatsu Akira、Ohtake Miki、Shimatani Zenpei、Nishida Keiji
- Journal Title
  
  Frontiers in Plant Science
  
  Volume: 11 Pages: 00925-00925
- DOI
  10.3389/fpls.2020.00925
- Data Source
  KAKENHI-PROJECT-17H04994
[Journal Article] Targeted Base Editing with CRISPR-Deaminase in Tomato2019
- Author(s)
  Shimatani Zenpei、Ariizumi Tohru、Fujikura Ushio、Kondo Akihiko、Ezura Hiroshi、Nishida Keiji
- Journal Title
  
  Methods Mol Biol.
  
  Volume: 1917 Pages: 297-307
- DOI
  10.1007/978-1-4939-8991-1_22
- ISBN
  9781493989904, 9781493989911
- Data Source
  KAKENHI-PROJECT-17H04994
[Journal Article] Mammalian synthetic biology by CRISPRs engineering and applications2019
- Author(s)
  Katayama Kenta、Mitsunobu Hitoshi、Nishida Keiji
- Journal Title
  
  Current Opinion in Chemical Biology
  
  Volume: 52 Pages: 79-84
- DOI
  10.1016/j.cbpa.2019.05.020
- Data Source
  KAKENHI-PROJECT-17H04994
[Journal Article] A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall2019
- Author(s)
  Furuhata Yuichi、Sakai Ayako、Murakami Tomi、Morikawa Mone、Nakamura Chikashi、Yoshizumi Takeshi、Fujikura Ushio、Nishida Keiji、Kato Yoshio
- Journal Title
  
  Scientific Reports
  
  Volume: 9 Issue: 1 Pages: 2163-2163
- DOI
  10.1038/s41598-018-38119-9
- NAID
  120006559318
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17H04994
[Journal Article] In vivo targeted single-nucleotide editing in zebrafish.2018
- Author(s)
  Tanaka, S., Yoshioka, S., Nishida, K., Hosokawa, H., Kakizuka, A., and Maegawa, S.
- Journal Title
  
  Sci. Rep.
  
  Volume: 8 Issue: 1 Pages: 11423-11423
- DOI
  10.1038/s41598-018-29794-9
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18J14073, KAKENHI-PROJECT-16H05151, KAKENHI-PROJECT-17H04994
[Journal Article] Deaminase-mediated multiplex genome editing in Escherichia coli2018
- Author(s)
  Banno S, Nishida K, Arazoe T, Mitsunobu H and Kondo A.
- Journal Title
  
  Nature Microbiology
  
  Volume: 3 Issue: 4 Pages: 423-429
- DOI
  10.1038/s41564-017-0102-6
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K17879, KAKENHI-PROJECT-17H05021, KAKENHI-PROJECT-16K14654, KAKENHI-PROJECT-17H04994
[Journal Article] Genetic and physiological basis for antibody production by Kluyveromyces marxianus2018
- Author(s)
  Nambu-Nishida Yumiko、Nishida Keiji、Hasunuma Tomohisa、Kondo Akihiko
- Journal Title
  
  AMB Express
  
  Volume: 8 Issue: 1 Pages: 56-56
- DOI
  10.1186/s13568-018-0588-1
- NAID
  120006462314
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16K14654, KAKENHI-PROJECT-17H04994
[Journal Article] Inheritance of co-edited genes by CRISPR-based targeted nucleotide substitutions in rice2018
- Author(s)
  Shimatani Zenpei、Fujikura Ushio、Ishii Hisaki、Matsui Yusuke、Suzuki Minoru、Ueke Yuki、Taoka Ken-ichiro、Terada Rie、Nishida Keiji、Kondo Akihiko
- Journal Title
  
  Plant Physiology and Biochemistry
  
  Volume: 131 Pages: 78-83
- DOI
  10.1016/j.plaphy.2018.04.028
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-17H04994, KAKENHI-PROJECT-16K07560
[Journal Article] Herbicide tolerance-assisted multiplex targeted nucleotide substitution in rice2018
- Author(s)
  Shimatani Zenpei、Fujikura Ushio、Ishii Hisaki、Terada Rie、Nishida Keiji、Kondo Akihiko
- Journal Title
  
  Data in Brief
  
  Volume: 20 Pages: 1325-1331
- DOI
  10.1016/j.dib.2018.08.124
- NAID
  120006543325
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17H04994
[Journal Article] Targeted nucleotide editing technologies for microbial metabolic engineering2018
- Author(s)
  Arazoe, T., Kondo, A., Nishida, K.
- Journal Title
  
  Biotechnology Journal
  
  Volume: 13 Issue: 9 Pages: 170596-170596
- DOI
  10.1002/biot.201700596
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K19218, KAKENHI-PROJECT-17H05021, KAKENHI-PROJECT-17H04994
[Journal Article] Glycosyltransferase MDR1 assembles a dividing ring for mitochondrial proliferation comprising polyglucan nanofilaments2017
- Author(s)
  Yoshida Yamato, Kuroiwa Haruko, Shimada Takashi, Yoshida Masaki, Ohnuma Mio, Fujiwara Takayuki, Imoto Yuuta, Yagisawa Fumi, Nishida Keiji, Hirooka Shunsuke, Misumi Osami, Mogi Yuko, Akakabe Yoshihiko, Matsushita Kazunobu, Kuroiwa Tsuneyoshi
- Journal Title
  
  Proceedings of the National Academy of Sciences
  
  Volume: 114 Issue: 50 Pages: 13284-13289
- DOI
  10.1073/pnas.1715008114
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-17K00676, KAKENHI-PROJECT-16H04813, KAKENHI-PROJECT-15K18588, KAKENHI-PROJECT-17H04994
[Journal Article] Beyond Native Cas9: Manipulating Genomic Information and Function2017
- Author(s)
  Mitsunobu Hitoshi、Teramoto Jun、Nishida Keiji、Kondo Akihiko
- Journal Title
  
  Trends in Biotechnology
  
  Volume: 35 Issue: 10 Pages: 983-996
- DOI
  10.1016/j.tibtech.2017.06.004
- Data Source
  KAKENHI-PROJECT-16K14654, KAKENHI-PROJECT-17H04994
[Journal Article] Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion2017
- Author(s)
  Shimatani, Z., Kashojiya, S., Takayama, M., Terada, R., Arazoe, T., Ishii, H., Teramura, H., Yamamoto, T., Komatsu, H., Miura, K., Ezura, H., Nishida, K., Ariizumi, T. and Kondo A.
- Journal Title
  
  Nature Biotechnology
  
  Volume: 印刷中 Issue: 5 Pages: 441-443
- DOI
  10.1038/nbt.3833
- Peer Reviewed / Acknowledgement Compliant
- Data Source
  KAKENHI-PROJECT-15K18647, KAKENHI-PROJECT-16K07560, KAKENHI-PROJECT-16K14654, KAKENHI-PUBLICLY-16H01458
[Journal Article] Development of a comprehensive set of tools for genome engineering in a cold- and thermo-tolerant Kluyveromyces marxianus yeast strain2017
- Author(s)
  Nambu-Nishida Yumiko、Nishida Keiji、Hasunuma Tomohisa、Kondo Akihiko
- Journal Title
  
  Scientific Reports
  
  Volume: 7 Issue: 1 Pages: 8993-8993
- DOI
  10.1038/s41598-017-08356-5
- NAID
  120006346142
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16K14654, KAKENHI-PROJECT-17H04994
[Journal Article] Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems2016
- Author(s)
  Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki, M., Hara, K.Y., Shimatani, Z. and Kondo, A.
- Journal Title
  
  Science
  
  Volume: 353 Issue: 6305 Pages: 6305-6305
- DOI
  10.1126/science.aaf8729
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-26430198, KAKENHI-PROJECT-15K18647, KAKENHI-PROJECT-16K14654, KAKENHI-PROJECT-15K18466, KAKENHI-PROJECT-15K14436
[Journal Article] Evaluation of genes involved in oxidative phosphorylation in yeast by developing a simple and rapid method to measure mitochondrial ATP synthetic activity.2015
- Author(s)
  Ye X, Morikawa K, Ho SH, Araki M, Nishida K, Hasunuma T, Hara KY, Kondo A.
- Journal Title
  
  Microbial Cell Factories
  
  Volume: 14 Issue: 1 Pages: 56-56
- DOI
  10.1186/s12934-015-0239-z
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PUBLICLY-26119710, KAKENHI-PROJECT-26430198, KAKENHI-PUBLICLY-26119711, KAKENHI-PUBLICLY-26117715
[Journal Article] Improvement of oxidized glutathione fermentation by thiol redox metabolism engineering in Saccharomyces cerevisiae.2015
- Author(s)
  Hara KY, Aoki N, Kobayashi J, Kiriyama K, Nishida K, Araki M, Kondo A.
- Journal Title
  
  Appl Microbiol Biotechnol.
  
  Volume: Nov;99(22) Pages: 9771-8
- Peer Reviewed
- Data Source
  KAKENHI-PUBLICLY-26119710
[Patent] 標的化したDNA配列の核酸塩基を特異的に変換する、単子葉植物のゲノム配列の変換方法、及びそれに用いる分子複合体2015
- Inventor(s)
  西田敬二、島谷善平、近藤昭彦
- Industrial Property Rights Holder
  国立大学法人神戸大学
- Industrial Property Rights Type
  特許
- Filing Date
  2015-11-27
- Data Source
  KAKENHI-PUBLICLY-26119710
[Patent] 標的化したＤＮＡ配列の核酸塩基を特異的に変換するゲノム配列の改変方法及びそれに用いる分子複合体2015
- Inventor(s)
  西田敬二、近藤昭彦、坂野聡美
- Industrial Property Rights Holder
  神戸大学
- Industrial Property Rights Type
  特許
- Filing Date
  2015-03-04
- Overseas
- Data Source
  KAKENHI-PUBLICLY-26119710
[Patent] 細胞内でＤＮＡを切断せずに脱塩基反応によりゲノム中の　　　　　　　狙った領域に変異を導入する技術2014
- Inventor(s)
  西田敬二、近藤昭彦
- Industrial Property Rights Holder
  神戸大学
- Industrial Property Rights Type
  特許
- Industrial Property Number
  2014-224745
- Filing Date
  2014-11-04
- Data Source
  KAKENHI-PUBLICLY-26119710
[Presentation] ゲノムを切らずに書き換える新たなゲノム編集技術の開発2015
- Author(s)
  西田敬二, 坂野聡美, 近藤昭彦
- Organizer
  第６７回日本生物工学会　大会
- Place of Presentation
  鹿児島県鹿児島市
- Year and Date
  2015-10-26
- Data Source
  KAKENHI-PUBLICLY-26119710
[Presentation] Targeted base exchange-mediated genome editing2015
- Author(s)
  西田敬二, 坂野聡美, 荒添貴之、島谷善平、近藤昭彦
- Organizer
  BMB2015
- Place of Presentation
  兵庫県神戸市
- Year and Date
  2015-12-03
- Int'l Joint Research
- Data Source
  KAKENHI-PUBLICLY-26119710
[Presentation] Pin-point genome editing without cleaving DNA2015
- Author(s)
  西田敬二
- Organizer
  植物ゲノム編集技術に関する国際シンポジウム
- Place of Presentation
  茨城県つくば市
- Year and Date
  2015-11-28
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PUBLICLY-26119710

1. BANNO Satomi

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
2. MISUMI Osami

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
3. ARAKI Michihiro

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
4. 大沼みお

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
5. 谷内江望

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
6. 荒添貴之

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results

Nishida Keiji 西田 敬二

NISHIDA KEIJI 西田 敬二

Research Projects

Research Products

Co-Researchers

Creation of artificial enzymes that enable targeted editing of any DNA basesPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Creation of self-evolving enzymatic system that enables semi rational design of genomePrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

DNA 塩基置換酵素の“逆進化”誘導によるDNAを切らないゲノム編集ツールの開発Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

[Book] 完全版 ゲノム編集実験スタンダード2019

Author(s)

Total Pages

Publisher

ISBN

Data Source

[Journal Article] CRISPR-derived genome editing technologies for metabolic engineering2021

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Efficient base editing in tomato using a highly expressed transient system2021

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Base editors for simultaneous introduction of C-to-T and A-to-G mutations2020

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Fruit setting rewires central metabolism via gibberellin cascades2020

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Multiple gene substitution by Target-AID base-editing technology in tomato2020

Author(s)

Journal Title

DOI

NAID

Data Source

[Journal Article] Production of Herbicide-Sensitive Strain to Prevent Volunteer Rice Infestation Using a CRISPR-Cas9 Cytidine Deaminase Fusion2020

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Targeted Base Editing with CRISPR-Deaminase in Tomato2019

Author(s)

Journal Title

DOI

ISBN

Data Source

[Journal Article] Mammalian synthetic biology by CRISPRs engineering and applications2019

Author(s)

Journal Title

DOI

Data Source

[Journal Article] A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall2019

Author(s)

Journal Title

DOI

NAID

Data Source

[Journal Article] In vivo targeted single-nucleotide editing in zebrafish.2018

Author(s)

Journal Title

Nishida Keiji 西田敬二

NISHIDA KEIJI 西田敬二

[Book] 完全版　ゲノム編集実験スタンダード2019