TOKUNAGA MASAO 徳永正雄

… Alternative Names

TOKUNAGA Masao 徳永正雄

徳永正雄トクナガマサオ

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Researcher Number	20112782
Other IDs
External Links
Affiliation (based on the past Project Information) *help	2015: 鹿児島大学, 農学部, プロジェクト研究員 2004 – 2013: 鹿児島大学, 農学部, 教授 1999 – 2001: 鹿児島大学, 農学部, 教授 1994 – 1995: 鹿児島大学, 農学部, 教授
Review Section/Research Field	Principal Investigator Applied molecular and cellular biology / 応用微生物学・応用生物化学
Keywords	Principal Investigator 好塩菌 / 可溶性 / 好塩性酵素 / Nucleoside diphosphate kinase / Halobacterium / 好塩性 / 高次構造形成 / 融合蛋白質 / 酸性アミノ酸 / 極限環境微生物 … More / DnaK / 資源利用 / 環境保全 / 環境汚染 / バイオロジクス / バイオテクノロジー / 好塩性タンパク質 / 応用微生物 / solubility / chimeric protein / nucleoside dinhosnhate kinase / halophilic bacteria / halophilic enzyme / キメラ蛋白質 / フォールディング / 蛋白質 / サブユニット / 変異 / キメラタンパク質 / nucleoside diphosphate kinase / Halophilic / Extremely halophilic archaea / クローニング / Nucleoside diphosphate Kinase / 高度好塩菌 / 好塩性微生物 / 金属結合タンパク質 / 可溶性タグ / scFv / Brevibacillus / 変性ストレス耐性 / タグ / 凝集耐性 / まき戻り / 可溶化 / 好塩性蛋白質 / 大腸菌発現系 / 封入体 / 好塩性α-アミラーゼ / 構造可逆性 / 高機能化 / 蛋白質発現 / 蛋白質・糖鎖工学 / GroEL / 分子シャペロン / 熱ショック / KAR2 / BiP / 酵母 / 分泌 Less

Recovery of heavy metal ions from brine-damaged area with denaturation-resistant halophilic proteins and halophiles.Principal Investigator
- Principal Investigator
  
  TOKUNAGA MASAO
- Project Period (FY)
  2015
- Research Category
  
  Grant-in-Aid for Challenging Exploratory Research
- Research Field
  
  Applied molecular and cellular biology
- Research Institution
  Kagoshima University
Solubilization-inducing tag for solubilization, development of higher function and medicine for proteins.Principal Investigator
- Principal Investigator
  
  TOKUNAGA MASAO
- Project Period (FY)
  2011 – 2013
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Applied molecular and cellular biology
- Research Institution
  Kagoshima University
Distinct way to prepare the hyper-stable and stress-tolerant enzymes-halophilic enzyme engineering and hyper-functional cell productionPrincipal Investigator
- Principal Investigator
  
  TOKUNAGA Masao
- Project Period (FY)
  2008 – 2010
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Applied molecular and cellular biology
- Research Institution
  Kagoshima University
Construction of super-stable enzymes : distinct reversibility of structure and its mechanismPrincipal Investigator
- Principal Investigator
  
  TOKUNAGA Masao
- Project Period (FY)
  2004 – 2007
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Applied molecular and cellular biology
- Research Institution
  Kagoshima University
Mechanisms of protein foldingand molecular breeding in halophilic enzymesPrincipal Investigator
- Principal Investigator
  
  TOKUNAGA Masao
- Project Period (FY)
  1999 – 2001
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  応用微生物学・応用生物化学
- Research Institution
  Kagoshima University, Faculty of Agriculture
好塩性分子シャペロンに関する研究Principal Investigator
- Principal Investigator
  
  徳永正雄
- Project Period (FY)
  1995
- Research Category
  
  Grant-in-Aid for Scientific Research on Priority Areas
- Research Institution
  Kagoshima University
異種蛋白質の高次構造形成と分泌に関わるBiP/Kar2蛋白の構造と機能Principal Investigator
- Principal Investigator
  
  徳永正雄
- Project Period (FY)
  1994
- Research Category
  
  Grant-in-Aid for Scientific Research on Priority Areas
- Research Institution
  Kagoshima University

All 2016 2013 2012 2011 2010 2009 2008 2007 2006 2005 Other

All Journal Article Presentation Book Patent

[Book] Halophilic Properties and their Manipulation and Application. in Extremophiles : Sustainable Resorces and Biotechnological Implications, First2013
- Author(s)
  Arakawa T. Tokunaga H. Ishibashi M. Tokunaga M.
- Publisher
  Edited by Om V. Singh (Wiley-Blackwell)
- Data Source
  KAKENHI-PROJECT-23580475
[Journal Article] Halophilic metal binding protein and its His-Asp repeating peptides as fusion partners for high solubility and affinity-purification of fusion proteins.2016
- Author(s)
  Rui Yamaguchi, Daiki Otsuji, Hiroko Tokunaga, Matsujiro Ishibashi, Tsutomu Arakawa, Masao Tokunaga
- Journal Title
  
  Bull. Soc. Sea Water Sci., Jpn.
  
  Volume: 70 Pages: 51-52
- NAID
  130006101481
- Peer Reviewed / Acknowledgement Compliant / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K14922
[Journal Article] Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner.2013
- Author(s)
  Tokunaga M, Mizukami M, Yamasaki K, Tokunaga H, Onishi H, Hanagata H, Ishibashi M, Miyauchi A, Tsumoto K, Arakawa T.
- Journal Title
  
  Applied Microbiology and Biotechnology
  
  Volume: 97 Issue: 19 Pages: 8569-8580
- DOI
  10.1007/s00253-013-4695-2
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23580475
[Journal Article] Amyloid fibril formation in vitro from halophilic metal binding protein : Its high solubility and reversibility minimized formation of amorphous protein aggregations2013
- Author(s)
  Tokunaga Y, Matsumoto M, Tokunaga M, Arakawa T, Sugimoto Y.
- Journal Title
  
  Protein Sci
  
  Volume: 22(11) Pages: 1582-1591
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23580475
[Journal Article] Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner2013
- Author(s)
  Tokunaga M, Mizukami M, Yamasaki K, Tokunaga H, Onishi H, Hanagata H, Ishibashi M, Miyauchi A, Tsumoto K, Arakawa T.
- Journal Title
  
  Appl Microbiol Biotechnol
  
  Volume: 97(19) Pages: 8569-8580
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23580475
[Journal Article] Halophilic characterization of starch-binding domain from Kocuria varians alpha-amylase2012
- Author(s)
  R. Yamaguchi, I. Inoue, H. Tokunaga, M. Ishibashi, T. Arakawa, J. Sumitani, T. Kawaguchi, M. Tokunaga
- Journal Title
  
  International Journal of Biological Macromolecules
  
  Volume: 50 Issue: 1 Pages: 95-102
- DOI
  10.1016/j.ijbiomac.2011.10.007
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23580475
[Journal Article] Halophilic characterization of starch-binding domain from Kocuria variansα-amylase2012
- Author(s)
  Yamaguchi R, Inoue Y, Tokunaga H, Ishibashi M, Arakawa T, Sumitani J, Kawaguchi T, Tokunaga M.
- Journal Title
  
  Int J Biol Macromol
  
  Volume: 50(1) Pages: 95-102
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23580475
[Journal Article] Halophilic β-lactamase as a new solubility-and folding-enhancing tag protein : Production of native human interleukin 1α and human neutrophil α-defensin2010
- Author(s)
  H.Tokunaga, S.Saito, K.Sakai, R.Yamaguchi, I.Katsuyama, T.Arakawa, K.Onozaki, T.Arakawa, M.Tokunaga
- Journal Title
  
  Applied Microbiology and Biotechnology 86
  
  Pages: 649-658
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580372
[Journal Article] Dimer-tetramer assembly of nucleoside diphosphate kinase from moderately halophilic bacterium Chromohalobacter salexigens DSM3043 : both residues 134 and 146 are critical for the tetrameric assembly2010
- Author(s)
  H. Tokunaga, K. Izutsu, S. Arai, Y. Yonezawa, R. Kuroki, T. Arakwa, and M. Tokunaga.
- Journal Title
  
  Enz. Microbial Technol
  
  Volume: 46 Pages: 129-135
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580372
[Journal Article] Salt-dependent thermo-reversible α-amylase : cloning and characterization of halophilic α-amylase from moderately halophilic bacterium, Kocuria varians.2010
- Author(s)
  Yamaguchi R, Tokunaga H, Ishibashi M, Arakawa T, Tokunaga M.
- Journal Title
  
  Appl Microbiol Biotechnol.
  
  Volume: 89 Pages: 673-684
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580372
[Journal Article] High solubility supports efficient refolding of thermally unfolded β-lactamase2010
- Author(s)
  Arakawa T, Tokunaga H, Yamaguchi R, Tokunaga M.
- Journal Title
  
  Int J Biol Macromol.
  
  Volume: 47 Pages: 706-709
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580372
[Journal Article] Halophilic beta-lactamase as a new solubilityand folding-enhancing tag protein : production of native human interleukin 1alpha and human neutrophil alpha-defensin2010
- Author(s)
  Tokunaga H, Saito S, Sakai K, Yamaguchi R, Katsuyama I, Arakawa T, Onozaki K, Arakawa T, Tokunaga M
- Journal Title
  
  Appl Microbiol Biotechnol
  
  Volume: 86(2) Pages: 649-658
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580372
[Journal Article] Engineering of halophilic enzymes : two acidic amino acid residues at the carboxy-terminal region confer halophilic characterstics to Halomonas and Pseudomonas nucleoside diphosphate kinases2008
- Author(s)
  Tokunaga, H., Arakawa, T., Tokunaga, M.
- Journal Title
  
  Protein Sciences
  
  Volume: 17 Pages: 1603-1610
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580372
[Journal Article] Residue 134 detrmines the dimer-tetramer assembly of nucleoside diphosphate kinase from moderately halophilic bacteria2008
- Author(s)
  Tokunaga H., Ishibashi, M., Arisaka, F., Arai, S., Kuroki, R., Arakawa, T., Tokunaga, M.
- Journal Title
  
  FEBS Lett 582
  
  Pages: 1049-1054
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16580280
[Journal Article] Engineering of halophilic enzymes : two acidic amino acid residues at the carboxy-terminal region confer halophilic characterstics to Halomonas and Pseudomonas nucleoside diphosphate kinases.2008
- Author(s)
  Tokunaga, H., Arakawa, T., Tokunaga, M.
- Journal Title
  
  Protein Sciences 17
  
  Pages: 1603-1610
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580372
[Journal Article] Dimeric structure of nucleoside diphosphate kinase from moderately halophilic bacterium : contrast to the tetrameric Pseudomonas counterpart2007
- Author(s)
  Y. Yonezawa, K. Izutsu, H. Tokunaga, H. Maeda, T. Arakawa, M. Tokunaga
- Journal Title
  
  FEMS Microbiol. Lett 268
  
  Pages: 52-58
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-16580280
[Journal Article] Dimeric structure of nucleoside diphosphate kinase from moderately halophhilic bacterium:contrast to the tetrameric Pseudomonas counterpart2007
- Author(s)
  Y.Yonezawa, K.Izutsu, H.Tokunaga, K.Maeda, T.Arakawa, and M.Tokunaga
- Journal Title
  
  FEMS Microbiol.Lett. 268
  
  Pages: 52-58
- Description
  「研究成果報告書概要(和文)」より
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16580280
[Journal Article] Contribution of halophilic nucleoside diphosphate kinase from Halomonas sp.593 to the heat stability of chimeric molecule2006
- Author(s)
  H.Tokunaga, Y.Oda, Y.Yonezawa, T.Arakawa, and M.Tokunaga, 525-530(2006)
- Journal Title
  
  ProteinPept.Lett. 13
  
  Pages: 525-530
- Description
  「研究成果報告書概要(和文)」より
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16580280
[Journal Article] Contribution of Halophilic Nucleoside Diphosphate Kinase Sequence to the Heat Stability of Chimeric Molecule2006
- Author(s)
  H.Tokunaga et al.
- Journal Title
  
  Protein ＆ Peptide Letters 13
  
  Pages: 525-530
- Data Source
  KAKENHI-PROJECT-16580280
[Journal Article] Contribution of halophilic nucleoside diphosphate kinase from Halomonas sp. 593 to the heat stability of chimeric molecule2006
- Author(s)
  H. Tokunaga, Y. Oda, Y. Yonezawa, T. Arakawa, M. Tokunaga
- Journal Title
  
  Protein Pept. Lett 13
  
  Pages: 525-530
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-16580280
[Journal Article] Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum2005
- Author(s)
  H.Besir, K.Zeth, A.Bracher, U.Heider, M.Ishibashi, M.Tokunaga, D.Oesterhelt
- Journal Title
  
  FEBS Lett. 579
  
  Pages: 6595-6600
- Description
  「研究成果報告書概要(和文)」より
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16580280
[Journal Article] Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum2005
- Author(s)
  H. Besir, K. Zeth, A. Bracher, U. Heider, M. Ishibashi, M. Tokunaga, D. Oesterhelt
- Journal Title
  
  FEBS Lett 579
  
  Pages: 6595-6600
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-16580280
[Patent] 融合タンパク質発現ベクター2005
- Inventor(s)
  徳永正雄他
- Industrial Property Rights Holder
  鹿児島大学
- Industrial Property Number
  2005-047809
- Filing Date
  2005-02-23
- Data Source
  KAKENHI-PROJECT-16580280
[Presentation] Brevibacillus発現系を用いた一本鎖抗体 (scFv) の発現生産2012
- Author(s)
  徳永正雄、山崎晃路、水上　誠、大西廣優、徳永廣子、石橋松二郎、花方　寛、宮内　明、荒川　力
- Organizer
  ２０１２年度　日本生化学会大会
- Place of Presentation
  福岡市
- Data Source
  KAKENHI-PROJECT-23580475
[Presentation] Brevibacillus発現系を用いた一本鎖抗体(scFv)の発現生産2012
- Author(s)
  徳永正雄、山崎晃路、水上誠、大西廣優、徳永廣子、石橋松二郎、花方寛、宮内明、荒川力
- Organizer
  生化学会2012年度大会
- Place of Presentation
  福岡県福岡市
- Data Source
  KAKENHI-PROJECT-23580475
[Presentation] 塩依存的にrefoldingする中度好塩菌Kocuria varians由来α-amylaseの性質検討とcloning及び発現2011
- Author(s)
  山口類、石橋松二郎、徳永廣子、荒川力、徳永正雄
- Organizer
  日本農芸化学会2011年大会
- Place of Presentation
  京都女子大学
- Year and Date
  2011-03-26
- Data Source
  KAKENHI-PROJECT-20580372
[Presentation] 塩依存的に巻き戻る中度好塩菌Kocuria varians由来α-アミラーゼの性質検討とクローニング2011
- Author(s)
  山口類、石橋松二郎、徳永廣子、荒川力、徳永正雄
- Organizer
  日第11回日本蛋白科学会年会
- Place of Presentation
  大阪府吹田市
- Data Source
  KAKENHI-PROJECT-23580475
[Presentation] 高度好塩菌由来nucleoside diphosphate kinase変異体G114Rの解析2010
- Author(s)
  石橋松二郎、岩佐達也、徳永正雄
- Organizer
  2010日本農芸化学会大会
- Place of Presentation
  東京
- Year and Date
  2010-03-28
- Data Source
  KAKENHI-PROJECT-20580372
[Presentation] 好塩性β-ラクタマーゼを可溶性タグとした融合蛋白質発現系の開発2010
- Author(s)
  山口類、徳永廣子、斎藤尚子、逆井一樹、勝山巌、荒川友博、小野崎菊夫、荒川力、徳永正雄
- Organizer
  2010日本農芸化学会大会
- Place of Presentation
  東京
- Year and Date
  2010-03-29
- Data Source
  KAKENHI-PROJECT-20580372
[Presentation] 好塩性付与蛋白質の分子育種2009
- Author(s)
  徳永廣子、荒川力、山口類、徳永正雄
- Organizer
  2009年日本農芸化学会大会
- Place of Presentation
  福岡
- Year and Date
  2009-03-28
- Data Source
  KAKENHI-PROJECT-20580372
[Presentation] 高い構造可逆性と新規二量体構造を示した中度好塩性細菌由来nucleoside diphosphate kinase2008
- Author(s)
  米澤悌、徳永廣子、前田浩貴、石橋松二郎、徳永正雄
- Organizer
  日本農芸化学会
- Place of Presentation
  名城大学
- Year and Date
  2008-03-27
- Data Source
  KAKENHI-PROJECT-16580280
[Presentation] 1アミノ酸(134th)置換による好塩性nueleoside diphosphate kinaseの2量体-4量体変換2008
- Author(s)
  徳永廣子, 石橋松二郎, 有坂文雄, 新井栄揮, 黒木良太, 山口類, 荒川力, 徳永正雄
- Organizer
  2008年日本蛋白質科学会年会
- Place of Presentation
  東京タワーホール船堀
- Data Source
  KAKENHI-PROJECT-20580372
[Presentation] Electrostatic and hydrophobic interactions play a major role in the stability and refolding of halophilic proteins2005
- Author(s)
  Masao Tokunaga, Matsujiro Ishibashi, Hiroko Tokunaga, and Tsutomu Arakawa
- Organizer
  International Symposium on Extremophiles and Their Applications
- Place of Presentation
  Tokyo
- Year and Date
  2005-11-29
- Description
  「研究成果報告書概要(和文)」より
- Data Source
  KAKENHI-PROJECT-16580280
[Presentation] 中度好塩菌由来金属結合蛋白質の金属親和性と塩の効果
- Author(s)
  尾辻大樹、西元愛里、石橋松二郎、徳永廣子、徳永正雄
- Organizer
  日本生化学会　九州支部大会
- Place of Presentation
  佐賀大学　農学部
- Data Source
  KAKENHI-PROJECT-23580475
[Presentation] 塩依存的にまき戻る中度好塩菌Kocuria varians由来αーアミラーゼの性質検討とクローニング
- Author(s)
  山口類、石橋松二郎、徳永廣子、荒川力、徳永正雄
- Organizer
  第１１回日本蛋白質科学会年会
- Place of Presentation
  ホテル阪急エキスポパーク　大阪府
- Data Source
  KAKENHI-PROJECT-23580475

1. ISHIBASHI Matsujiro (20305163)

# of Collaborated Projects: 4 results

# of Collaborated Products: 19 results
2. TOKUNAGA Hiroko (60381191)

# of Collaborated Projects: 2 results

# of Collaborated Products: 23 results
3. 小林武一 (50041645)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results

TOKUNAGA MASAO 徳永 正雄

TOKUNAGA Masao 徳永 正雄

徳永 正雄 トクナガ マサオ

Research Projects

Research Products

Co-Researchers

Recovery of heavy metal ions from brine-damaged area with denaturation-resistant halophilic proteins and halophiles.Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Solubilization-inducing tag for solubilization, development of higher function and medicine for proteins.Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Distinct way to prepare the hyper-stable and stress-tolerant enzymes-halophilic enzyme engineering and hyper-functional cell productionPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Construction of super-stable enzymes : distinct reversibility of structure and its mechanismPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Mechanisms of protein foldingand molecular breeding in halophilic enzymesPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

好塩性分子シャペロンに関する研究Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Institution

異種蛋白質の高次構造形成と分泌に関わるBiP/Kar2蛋白の構造と機能Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Institution

[Book] Halophilic Properties and their Manipulation and Application. in Extremophiles : Sustainable Resorces and Biotechnological Implications, First2013

Author(s)

Publisher

Data Source

[Journal Article] Halophilic metal binding protein and its His-Asp repeating peptides as fusion partners for high solubility and affinity-purification of fusion proteins.2016

Author(s)

Journal Title

NAID

Data Source

[Journal Article] Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner.2013

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Amyloid fibril formation in vitro from halophilic metal binding protein : Its high solubility and reversibility minimized formation of amorphous protein aggregations2013

Author(s)

Journal Title

Data Source

[Journal Article] Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner2013

Author(s)

Journal Title

Data Source

[Journal Article] Halophilic characterization of starch-binding domain from Kocuria varians alpha-amylase2012

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Halophilic characterization of starch-binding domain from Kocuria variansα-amylase2012

Author(s)

Journal Title

Data Source

[Journal Article] Halophilic β-lactamase as a new solubility-and folding-enhancing tag protein : Production of native human interleukin 1α and human neutrophil α-defensin2010

Author(s)

Journal Title

TOKUNAGA MASAO 徳永正雄

TOKUNAGA Masao 徳永正雄

徳永正雄トクナガマサオ