ISHIBASHI Matsujiro 石橋松二郎

Researcher Number	20305163
Other IDs
External Links
Affiliation (Current)	2025: 鹿児島大学, 農水産獣医学域農学系, 教授
Affiliation (based on the past Project Information) *help	2007 – 2013: Kagoshima University, Faculty of Agriculture, Associate Professor 2006: 鹿児島大学, 農学部, 助教授 1999 – 2005: 鹿児島大学, 農学部, 助手
Review Section/Research Field	Principal Investigator 生物資源科学 Except Principal Investigator Applied molecular and cellular biology / 応用微生物学・応用生物化学
Keywords	Principal Investigator folding / ヌクレオシド二リン酸キナーゼ / 好塩性酵素 / 高度好塩菌 Except Principal Investigator 可溶性 / 好塩性酵素 / 好塩菌 / Nucleoside diphosphate kinase / Halobacterium / 好塩性 … More / 高次構造形成 / 融合蛋白質 / 酸性アミノ酸 / solubility / chimeric protein / nucleoside dinhosnhate kinase / halophilic bacteria / halophilic enzyme / キメラ蛋白質 / フォールディング / 蛋白質 / サブユニット / 変異 / キメラタンパク質 / nucleoside diphosphate kinase / Halophilic / Extremely halophilic archaea / DnaK / クローニング / Nucleoside diphosphate Kinase / 高度好塩菌 / 好塩性微生物 / 金属結合タンパク質 / 可溶性タグ / scFv / Brevibacillus / 変性ストレス耐性 / タグ / 凝集耐性 / まき戻り / 可溶化 / 好塩性蛋白質 / 大腸菌発現系 / 封入体 / 好塩性α-アミラーゼ / 構造可逆性 / 高機能化 / 蛋白質発現 / 極限環境微生物 / 蛋白質・糖鎖工学 Less

Solubilization-inducing tag for solubilization, development of higher function and medicine for proteins.
- Principal Investigator
  
  TOKUNAGA MASAO
- Project Period (FY)
  2011 – 2013
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Applied molecular and cellular biology
- Research Institution
  Kagoshima University
Distinct way to prepare the hyper-stable and stress-tolerant enzymes-halophilic enzyme engineering and hyper-functional cell production
- Principal Investigator
  
  TOKUNAGA Masao
- Project Period (FY)
  2008 – 2010
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Applied molecular and cellular biology
- Research Institution
  Kagoshima University
Construction of super-stable enzymes : distinct reversibility of structure and its mechanism
- Principal Investigator
  
  TOKUNAGA Masao
- Project Period (FY)
  2004 – 2007
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Applied molecular and cellular biology
- Research Institution
  Kagoshima University
好塩性酵素を産業に生かすPrincipal Investigator
- Principal Investigator
  
  石橋松二郎
- Project Period (FY)
  2002 – 2004
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  生物資源科学
- Research Institution
  Kagoshima University
Mechanisms of protein foldingand molecular breeding in halophilic enzymes
- Principal Investigator
  
  TOKUNAGA Masao
- Project Period (FY)
  1999 – 2001
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  応用微生物学・応用生物化学
- Research Institution
  Kagoshima University, Faculty of Agriculture

All 2013 2012 2011 2010 2008 2005 2004 Other

All Journal Article Presentation Book

[Book] Halophilic Properties and their Manipulation and Application. in Extremophiles : Sustainable Resorces and Biotechnological Implications, First2013
- Author(s)
  Arakawa T. Tokunaga H. Ishibashi M. Tokunaga M.
- Publisher
  Edited by Om V. Singh (Wiley-Blackwell)
- Data Source
  KAKENHI-PROJECT-23580475
[Journal Article] Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner.2013
- Author(s)
  Tokunaga M, Mizukami M, Yamasaki K, Tokunaga H, Onishi H, Hanagata H, Ishibashi M, Miyauchi A, Tsumoto K, Arakawa T.
- Journal Title
  
  Applied Microbiology and Biotechnology
  
  Volume: 97 Issue: 19 Pages: 8569-8580
- DOI
  10.1007/s00253-013-4695-2
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23580475
[Journal Article] Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner2013
- Author(s)
  Tokunaga M, Mizukami M, Yamasaki K, Tokunaga H, Onishi H, Hanagata H, Ishibashi M, Miyauchi A, Tsumoto K, Arakawa T.
- Journal Title
  
  Appl Microbiol Biotechnol
  
  Volume: 97(19) Pages: 8569-8580
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23580475
[Journal Article] Halophilic characterization of starch-binding domain from Kocuria varians alpha-amylase2012
- Author(s)
  R. Yamaguchi, I. Inoue, H. Tokunaga, M. Ishibashi, T. Arakawa, J. Sumitani, T. Kawaguchi, M. Tokunaga
- Journal Title
  
  International Journal of Biological Macromolecules
  
  Volume: 50 Issue: 1 Pages: 95-102
- DOI
  10.1016/j.ijbiomac.2011.10.007
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23580475
[Journal Article] Halophilic characterization of starch-binding domain from Kocuria variansα-amylase2012
- Author(s)
  Yamaguchi R, Inoue Y, Tokunaga H, Ishibashi M, Arakawa T, Sumitani J, Kawaguchi T, Tokunaga M.
- Journal Title
  
  Int J Biol Macromol
  
  Volume: 50(1) Pages: 95-102
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-23580475
[Journal Article] Salt-dependent thermo-reversible α-amylase : cloning and characterization of halophilic α-amylase from moderately halophilic bacterium, Kocuria varians.2010
- Author(s)
  Yamaguchi R, Tokunaga H, Ishibashi M, Arakawa T, Tokunaga M.
- Journal Title
  
  Appl Microbiol Biotechnol.
  
  Volume: 89 Pages: 673-684
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580372
[Journal Article] Residue 134 detrmines the dimer-tetramer assembly of nucleoside diphosphate kinase from moderately halophilic bacteria2008
- Author(s)
  Tokunaga H., Ishibashi, M., Arisaka, F., Arai, S., Kuroki, R., Arakawa, T., Tokunaga, M.
- Journal Title
  
  FEBS Lett 582
  
  Pages: 1049-1054
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16580280
[Journal Article] Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum2005
- Author(s)
  H. Besir, K. Zeth, A. Bracher, U. Heider, M. Ishibashi, M. Tokunaga, D. Oesterhelt
- Journal Title
  
  FEBS Lett 579
  
  Pages: 6595-6600
- Description
  「研究成果報告書概要(欧文)」より
- Data Source
  KAKENHI-PROJECT-16580280
[Journal Article] Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum2005
- Author(s)
  H.Besir, K.Zeth, A.Bracher, U.Heider, M.Ishibashi, M.Tokunaga, D.Oesterhelt
- Journal Title
  
  FEBS Lett. 579
  
  Pages: 6595-6600
- Description
  「研究成果報告書概要(和文)」より
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16580280
[Journal Article] Facilitated folding and subunit assembly in Escherichia coli and in vitro of nucleoside diphosphate kinase from extremely halophilic archaeon conferred by amino-terminal extension containing hexa-His-tag2004
- Author(s)
  Matsujiro Ishibashi et al.
- Journal Title
  
  FEBS Letters 570
  
  Pages: 87-92
- Data Source
  KAKENHI-PROJECT-14760211
[Presentation] Brevibacillus発現系を用いた一本鎖抗体 (scFv) の発現生産2012
- Author(s)
  徳永正雄、山崎晃路、水上　誠、大西廣優、徳永廣子、石橋松二郎、花方　寛、宮内　明、荒川　力
- Organizer
  ２０１２年度　日本生化学会大会
- Place of Presentation
  福岡市
- Data Source
  KAKENHI-PROJECT-23580475
[Presentation] Brevibacillus発現系を用いた一本鎖抗体(scFv)の発現生産2012
- Author(s)
  徳永正雄、山崎晃路、水上誠、大西廣優、徳永廣子、石橋松二郎、花方寛、宮内明、荒川力
- Organizer
  生化学会2012年度大会
- Place of Presentation
  福岡県福岡市
- Data Source
  KAKENHI-PROJECT-23580475
[Presentation] 塩依存的にrefoldingする中度好塩菌Kocuria varians由来α-amylaseの性質検討とcloning及び発現2011
- Author(s)
  山口類、石橋松二郎、徳永廣子、荒川力、徳永正雄
- Organizer
  日本農芸化学会2011年大会
- Place of Presentation
  京都女子大学
- Year and Date
  2011-03-26
- Data Source
  KAKENHI-PROJECT-20580372
[Presentation] 塩依存的に巻き戻る中度好塩菌Kocuria varians由来α-アミラーゼの性質検討とクローニング2011
- Author(s)
  山口類、石橋松二郎、徳永廣子、荒川力、徳永正雄
- Organizer
  日第11回日本蛋白科学会年会
- Place of Presentation
  大阪府吹田市
- Data Source
  KAKENHI-PROJECT-23580475
[Presentation] 高度好塩菌由来nucleoside diphosphate kinase変異体G114Rの解析2010
- Author(s)
  石橋松二郎、岩佐達也、徳永正雄
- Organizer
  2010日本農芸化学会大会
- Place of Presentation
  東京
- Year and Date
  2010-03-28
- Data Source
  KAKENHI-PROJECT-20580372
[Presentation] 高い構造可逆性と新規二量体構造を示した中度好塩性細菌由来nucleoside diphosphate kinase2008
- Author(s)
  米澤悌、徳永廣子、前田浩貴、石橋松二郎、徳永正雄
- Organizer
  日本農芸化学会
- Place of Presentation
  名城大学
- Year and Date
  2008-03-27
- Data Source
  KAKENHI-PROJECT-16580280
[Presentation] 1アミノ酸(134th)置換による好塩性nueleoside diphosphate kinaseの2量体-4量体変換2008
- Author(s)
  徳永廣子, 石橋松二郎, 有坂文雄, 新井栄揮, 黒木良太, 山口類, 荒川力, 徳永正雄
- Organizer
  2008年日本蛋白質科学会年会
- Place of Presentation
  東京タワーホール船堀
- Data Source
  KAKENHI-PROJECT-20580372
[Presentation] Electrostatic and hydrophobic interactions play a major role in the stability and refolding of halophilic proteins2005
- Author(s)
  Masao Tokunaga, Matsujiro Ishibashi, Hiroko Tokunaga, and Tsutomu Arakawa
- Organizer
  International Symposium on Extremophiles and Their Applications
- Place of Presentation
  Tokyo
- Year and Date
  2005-11-29
- Description
  「研究成果報告書概要(和文)」より
- Data Source
  KAKENHI-PROJECT-16580280
[Presentation] 中度好塩菌由来金属結合蛋白質の金属親和性と塩の効果
- Author(s)
  尾辻大樹、西元愛里、石橋松二郎、徳永廣子、徳永正雄
- Organizer
  日本生化学会　九州支部大会
- Place of Presentation
  佐賀大学　農学部
- Data Source
  KAKENHI-PROJECT-23580475
[Presentation] 塩依存的にまき戻る中度好塩菌Kocuria varians由来αーアミラーゼの性質検討とクローニング
- Author(s)
  山口類、石橋松二郎、徳永廣子、荒川力、徳永正雄
- Organizer
  第１１回日本蛋白質科学会年会
- Place of Presentation
  ホテル阪急エキスポパーク　大阪府
- Data Source
  KAKENHI-PROJECT-23580475

1. TOKUNAGA Masao (20112782)

# of Collaborated Projects: 4 results

# of Collaborated Products: 19 results
2. TOKUNAGA Hiroko (60381191)

# of Collaborated Projects: 2 results

# of Collaborated Products: 14 results

ISHIBASHI Matsujiro 石橋 松二郎

Research Projects

Research Products

Co-Researchers

Solubilization-inducing tag for solubilization, development of higher function and medicine for proteins.

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Distinct way to prepare the hyper-stable and stress-tolerant enzymes-halophilic enzyme engineering and hyper-functional cell production

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Construction of super-stable enzymes : distinct reversibility of structure and its mechanism

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

好塩性酵素を産業に生かすPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Mechanisms of protein foldingand molecular breeding in halophilic enzymes

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Book] Halophilic Properties and their Manipulation and Application. in Extremophiles : Sustainable Resorces and Biotechnological Implications, First2013

Author(s)

Publisher

Data Source

[Journal Article] Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner.2013

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner2013

Author(s)

Journal Title

Data Source

[Journal Article] Halophilic characterization of starch-binding domain from Kocuria varians alpha-amylase2012

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Halophilic characterization of starch-binding domain from Kocuria variansα-amylase2012

Author(s)

Journal Title

Data Source

[Journal Article] Salt-dependent thermo-reversible α-amylase : cloning and characterization of halophilic α-amylase from moderately halophilic bacterium, Kocuria varians.2010

Author(s)

Journal Title

Data Source

[Journal Article] Residue 134 detrmines the dimer-tetramer assembly of nucleoside diphosphate kinase from moderately halophilic bacteria2008

Author(s)

Journal Title

Data Source

[Journal Article] Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum2005

Author(s)

Journal Title

Description

Data Source

[Journal Article] Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum2005

Author(s)

Journal Title

Description

Data Source

[Journal Article] Facilitated folding and subunit assembly in Escherichia coli and in vitro of nucleoside diphosphate kinase from extremely halophilic archaeon conferred by amino-terminal extension containing hexa-His-tag2004

Author(s)

Journal Title

Data Source

[Presentation] Brevibacillus発現系を用いた一本鎖抗体 (scFv) の発現生産2012

Author(s)

ISHIBASHI Matsujiro 石橋松二郎