Hashii Noritaka 橋井則貴

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HASHII Noritaka 橋井則貴

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Researcher Number	20425672
Other IDs
Affiliation (Current)	2025: 国立医薬品食品衛生研究所, 生物薬品部, 室長
Affiliation (based on the past Project Information) *help	2021 – 2024: 国立医薬品食品衛生研究所, 生物薬品部, 室長 2010 – 2018: 国立医薬品食品衛生研究所, 生物薬品部, 室長 2011: 国立医薬品食品衛生研究所, 生物薬品部, 第一室長 2008 – 2009: National Institute of Health Sciences, 生物薬品部, 主任研究官
Review Section/Research Field	Principal Investigator Physical pharmacy / Drug development chemistry Except Principal Investigator Basic Section 47060:Clinical pharmacy-related / Medical pharmacy
Keywords	Principal Investigator 糖タンパク質 / 水素重水素交換／質量分析 / 質量分析 / 糖鎖 / 再生医療 / 再生医学 / 医薬品化学 / HDX/MS / 水素重水素交換 / 相互作用解析 … More / 水素／重水素交換質量分析 / 抗TNFα抗体 / 分子標的薬 / 水素重水素交換質量分析 / エピトープ / 相互作用 / 水素/重水素交換質量分析 / 抗TNF-α抗体 / 高次構造 / 糖鎖生物学 / 分析化学 / 神経分化 / 間葉系幹細胞 … More Except Principal Investigator 抗体医薬品 / FcRn / MAPPs / in vitro recycling assay / 抗原提示 / 副作用 / 医薬品 / 中毒性表皮壊死症 / スティーブンス・ジョンソン症候群 / 抗てんかん薬 / 解熱鎮痛剤 / ペプチド / 重篤副作用 / HLA / 中毒性表皮壊死症（TEN） / スティーブンス・ジョンソン症候群（SJS） / 水素重水素交換／質量分析 / HDX/MS / アミノ酸改変 / 半減期延長 / Fcγ受容体 / 表面プラズモン共鳴 Less

体内分布や抗原提示に着目したFcRn親和性改変抗体間の違いに関する研究
- Principal Investigator
  
  鈴木琢雄
- Project Period (FY)
  2024 – 2026
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 47060:Clinical pharmacy-related
- Research Institution
  National Institute of Health Sciences
MAPPs assay to elucidate the influences of the change of FcRn affinity on the antigen presentation.
- Principal Investigator
  
  Suzuki Takuo
- Project Period (FY)
  2021 – 2023
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 47060:Clinical pharmacy-related
- Research Institution
  National Institute of Health Sciences
HLAの関与する解熱鎮痛薬誘因性重症薬疹の初期発症メカニズムの解明
- Principal Investigator
  
  岡本好海 (内田)
- Project Period (FY)
  2017 – 2018
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Medical pharmacy
- Research Institution
  National Institute of Health Sciences
Development of analytical method for glycoprotein-glycan interaction by hydrogen/deuterium exchange mass spectrometryPrincipal Investigator
- Principal Investigator
  
  Hashii Noritaka
- Project Period (FY)
  2015 – 2017
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Physical pharmacy
- Research Institution
  National Institute of Health Sciences
Basic research on neonatal Fc receptor (FcRn) affinity to establish half-life extension technology of therapeutic antibodies
- Principal Investigator
  
  Suzuki Takuo
- Project Period (FY)
  2015 – 2017
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Medical pharmacy
- Research Institution
  National Institute of Health Sciences
Development of technique for higher order structure of glycoprotein by hydrogen/deuterium exchange mass spectrometryPrincipal Investigator
- Principal Investigator
  
  HASHII Noritaka
- Project Period (FY)
  2012 – 2014
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Physical pharmacy
- Research Institution
  National Institute of Health Sciences
Development of evaluation methods for comparability of mesenchymal stem cells based on glycansPrincipal Investigator
- Principal Investigator
  
  HASHII Noritaka
- Project Period (FY)
  2010 – 2011
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Drug development chemistry
- Research Institution
  National Institute of Health Sciences
Study on neural differentiation of human mesenchymal stem cells based on glycansPrincipal Investigator
- Principal Investigator
  
  HASHII Noritaka
- Project Period (FY)
  2008 – 2009
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Drug development chemistry
- Research Institution
  National Institute of Health Sciences

All 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 Other

All Journal Article Presentation Book

[Book] HPLC, GCの測定条件設定テクニックと解析　事例集2016
- Author(s)
  橋井則貴
- Total Pages
  2
- Publisher
  （株）技術情報協会
- Data Source
  KAKENHI-PROJECT-15K07916
[Book] 蛋白質核酸酵素(糖鎖異常の網羅的解析, 臨時増刊号, 糖鎖情報の独自性と普遍性, 53)2008
- Author(s)
  川崎ナナ, 橋井則貴, 山口照英
- Data Source
  KAKENHI-PROJECT-20790110
[Journal Article] Change in N-Glycosylation of Plasma Proteins in Japanese Semisupercentenarians2015
- Author(s)
  Miura Y, Hashii N, Tsumoto H, Takakura D, Ohta Y, Abe Y, Arai Y, Kawasaki N, Hirose N, Endo T.
- Journal Title
  
  PLoS One
  
  Volume: 10
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-15K07916
[Journal Article] Enrichment of O-GlcNAc-modified peptides using novel thiol-alkyne and thiol-disulfide exchange2015
- Author(s)
  Tsumoto H, Ogasawara D, Hashii N, Suzuki T, Akimoto Y, Endo T, Miura Y.
- Journal Title
  
  Bioorg Med Chem Lett.
  
  Volume: 25 Pages: 2645-2649
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-15K07916
[Journal Article] Analysis of the local dynamics of human insulin and a rapid-acting insulin analog by hydrogen/deuterium exchange mass spectrometry2013
- Author(s)
  Nakazawa S, Ahn J, Hashii N, Hirose K, Kawasaki N
- Journal Title
  
  Biochim. Biophys. Acta
  
  Volume: 1834 Pages: 1210-1214
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24590069
[Journal Article] α1-3/4 fucosylation at Asn 241 ofβ-haptoglobin is a novel marker for colon cancer : A combinatorial approach for development of glycan biomarkers2012
- Author(s)
  S. Y. Park, S. H. Lee, N. Kawasaki, S. Itoh, K. Kang, Ryu S. Hee, N. Hashii, J. M. Kim, J. Y. Kim, Kim J. Hoe, Kim
- Journal Title
  
  Int. J. Cancer
  
  Volume: 130 Pages: 2366-2376
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22790125
[Journal Article] Glycomic analysis by liquid chromatography/mass spectrometry2012
- Author(s)
  N. Hashii, S. Nakazawa, N. Kawasaki
- Journal Title
  
  YAKUGAKU ZASSHI
  
  Volume: 132 Pages: 489-497
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22790125
[Journal Article] α1-3/4 fucosylation at Asn 241 of β-haptoglobin is a novel marker for colon cancer : A combinatorial approach for development of glycan biomarkers2012
- Author(s)
  Park, S.Y., Lee, S.H., Kawasaki, N., Itoh, S., Kang, K., Hee, Ryu, S., Hashii, N., Kim, J.M., Kim, J.Y., Hoe, Kim, J.
- Journal Title
  
  Int.J.Cancer
  
  Volume: 130 Pages: 2366-2376
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22790125
[Journal Article] Alteration of N-glycosylation in the kidney of systemic lupus erythematosus model mouse. Relative quantification of N-glycans by using isotope tagging method.2009
- Author(s)
  Noritaka Hashii, Nana Kawasalci, Satsuki Itoh, Yukari Nakajima, Toru Kawanishi, Teruhide Yamaguchi
- Journal Title
  
  Immunology 126
  
  Pages: 336-345
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790110
[Journal Article] Alteration of N-glycosylation in the kidney in a mouse model of systemic lupus erythematosus: relative quantification of N-glycans using an isotope-tagging method.2009
- Author(s)
  Hashii, N., Kawasaki, N., Itoh, S., Nakajima, Y., Kawanishi, T., Yamaguchi, T.
- Journal Title
  
  Immunology 126
  
  Pages: 336-345
- Data Source
  KAKENHI-PROJECT-20790110
[Journal Article] Glycosylation analysis of IgLON family proteins in rat brain by liquid chromatography and multiple-stage mass spectrometry.2009
- Author(s)
  Itoh, S., Hachisuka, A., Kawasaki, N., Hashii, N., Teshima, R., Hayakawa, T., Kawanishi, T., Yamaguchi, T.
- Journal Title
  
  Biochemistry 47
  
  Pages: 10132-10154
- Data Source
  KAKENHI-PROJECT-20790110
[Journal Article] Identification of glycoproteins carrying a target glycan-motif by liquid chromatography/multiple-stage mass spectrometry: identification of Lewis x-conjugated glycoproteins in mouse kidney.2009
- Author(s)
  Hashii, N., Kawasaki, N., Itoh, S., Nakajima, Y., Harazono, A., Kawanishi, T., Yamaguchi, T.
- Journal Title
  
  J. Proteome Res. 8
  
  Pages: 3415-3429
- Data Source
  KAKENHI-PROJECT-20790110
[Journal Article] Identification of glycoproteins carrying a target glycan-motif by liquid chromatography/multiple-stage mass spectrometry. Identification of Lewis x-glycoproteins in mouse kidney.2009
- Author(s)
  Noritaka Hashii, Nana Kawasaki, Satsuki ltoh, Yukari Nakajima, Akira Harazono, Toru Kawanishi, Teruhide Yamaguchi
- Journal Title
  
  J.Proteome Res. 8
  
  Pages: 3415-3429
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790110
[Journal Article] Simultaneous glycosylation analysis of human serum glycoproteins by high-performance liquid chromatography/tandem mass spectrometry.2008
- Author(s)
  Harazono, A., Kawasaki, N., Itoh, S., Hashii, N., Matsuishi-Nakajima, Y., Kawanishi, T., Yamaguchi, T.
- Journal Title
  
  J Chromatogr B Analyt Technol Biomed Life Sci. 869
  
  Pages: 20-30
- NAID
  10028001892
- Data Source
  KAKENHI-PROJECT-20790110
[Presentation] 抗体のFcRn親和性改変がFcγ受容体結合性や高次構造に及ぼす影響2017
- Author(s)
  鈴木琢雄、東阪嘉子、橋井則貴、多田　稔、岩崎紀之、石井明子
- Organizer
  第17回日本蛋白質科学会年会
- Data Source
  KAKENHI-PROJECT-15K08087
[Presentation] 水素重水素交換／質量分析法の糖鎖―タンパク質間相互作用解析への応用2016
- Author(s)
  橋井則貴，鈴木琢雄，蛭田葉子，石井明子
- Organizer
  第89回日本生化学会大会
- Place of Presentation
  仙台
- Year and Date
  2016-09-26
- Data Source
  KAKENHI-PROJECT-15K07916
[Presentation] FcRn親和性の違いが抗体医薬品の体内分布などに及ぼす影響2016
- Author(s)
  鈴木琢雄、宮崎ちひろ、多田稔、橋井則貴、石井明子
- Organizer
  日本薬学会第136年会
- Place of Presentation
  横浜
- Year and Date
  2016-03-27
- Data Source
  KAKENHI-PROJECT-15K08087
[Presentation] 水素/重水素交換および質量分析法を利用したtumor necrosis factor alpha(TNFα)アンタゴニストとTNFαの相互作用解析2014
- Author(s)
  中澤志織，橋井則貴，川崎ナナ
- Organizer
  日本薬学会第134年会
- Place of Presentation
  熊本
- Data Source
  KAKENHI-PROJECT-24590069
[Presentation] 水素/重水素交換質量分析を用いたバイオ医薬品の高次構造解析2014
- Author(s)
  橋井則貴
- Organizer
  第5回生体相互作用解析フォーラム
- Place of Presentation
  大阪大学
- Year and Date
  2014-04-22
- Invited
- Data Source
  KAKENHI-PROJECT-24590069
[Presentation] 抗体医薬品の分子変化体モニターリングシステム2014
- Author(s)
  太田悠葵, 橋井則貴, 原園景, 鈴木琢雄, 川崎ナナ
- Organizer
  日本薬学会第134年会
- Place of Presentation
  熊本
- Data Source
  KAKENHI-PROJECT-24590069
[Presentation] Analysis of interaction between TNFα and anti-TNFα agents using hydrogen/deuterium exchange and mass spectrometry2013
- Author(s)
  中澤志織，橋井則貴，川崎ナナ
- Organizer
  第86回日本生化学会
- Place of Presentation
  横浜
- Data Source
  KAKENHI-PROJECT-24590069
[Presentation] Analysis of interaction between TNF-alpha and anti-TNF-alpha agents using hydrogen/deuterium exchange and mass spectrometry2013
- Author(s)
  Nakazawa S, Hashii N, Kawasaki N
- Organizer
  61st ASMS Conference on Mass Spectrometry and Allied Topics
- Place of Presentation
  Minneapolis, USA
- Data Source
  KAKENHI-PROJECT-24590069
[Presentation] 水素重水素交換／質量分析によるヒトインスリンアナログの多量体安定性に関与する部位の特定2012
- Author(s)
  橋井則貴，中澤志織， Joomi Ahn，廣瀬賢治，川崎ナナ
- Organizer
  第85 回日本生化学会大会
- Place of Presentation
  福岡
- Data Source
  KAKENHI-PROJECT-24590069
[Presentation] Analysis of interaction between TNF-α and anti-TNF-α agents by hydrogen deuterium exchange/mass　spectrometry2012
- Author(s)
  Nakazawa S, Hashii N, Kawasaki N.
- Organizer
  19th International Mass Spectrometry Conference
- Place of Presentation
  京都
- Data Source
  KAKENHI-PROJECT-24590069
[Presentation] 液体クロマトグラフィー/質量分析及び主成分分析による糖鎖プロファイルのデータマイニング法の評価2011
- Author(s)
  橋井則貴,原園景,栗林亮佑,中澤志織,川崎ナナ
- Organizer
  第59回質量分析総合討論会
- Place of Presentation
  ホテル阪急エキスポパーク(大阪府)
- Year and Date
  2011-09-15
- Data Source
  KAKENHI-PROJECT-22790125
[Presentation] 糖鎖プロファイルによる間葉系幹細胞の分化初期における細胞の識別2011
- Author(s)
  橋井則貴,黄笑宇,栗林亮佑,川崎ナナ
- Organizer
  第84回日本生化学会大会
- Place of Presentation
  国立京都国際会館(京都府)
- Year and Date
  2011-09-22
- Data Source
  KAKENHI-PROJECT-22790125
[Presentation] 糖タンパク質医薬品の単糖試験法の標準化.2010
- Author(s)
  原園景, 小林哲, 川崎ナナ, 伊藤さつき, 多田稔, 橋井則貴, 石井明子, 荒戸照世, 柳原繁弘, 八木有紀, 古賀明子, 津田祐里子, 木村美紀子, 崎田政志, 北村智, 山口秀人, 三村尚志, 村田芳美, 濱詰康樹, 佐藤貴之, 長束俊治, 掛樋一晃, 木下充弘, 渡部沙木絵, 山口照英
- Organizer
  日本薬学会第130年会
- Data Source
  KAKENHI-PROJECT-20790110
[Presentation] Differential glycan analysis during neural differentiation of human mesenchymal stem cells by quantitative glycan profiling using liquid chromatography/mass spectrometry2010
- Author(s)
  N. Hashii, X. Huang, N. Kawasaki, T. Yamaguchi
- Organizer
  The 25^<th> International Carbohydrate Symposium(ICS2010)
- Place of Presentation
  幕張メッセ(千葉県)
- Year and Date
  2010-08-05
- Data Source
  KAKENHI-PROJECT-22790125
[Presentation] Identification of cell therapeutic products from stem cells based on glycans2010
- Author(s)
  N. Hashii, X. Huang, N. Kawasaki
- Organizer
  第33回日本分子生物学会年会第83回日本生化学会大会合同大会
- Place of Presentation
  神戸ポートアイランド(兵庫県)
- Year and Date
  2010-12-07
- Data Source
  KAKENHI-PROJECT-22790125
[Presentation] Quantitative glycan analysis during neural differentiation of human mesenchymal stem cells by liquid chromatography/mass spectrometry2010
- Author(s)
  N. Hashii, X. Huang, N. Kawasaki, T. Yamaguchi
- Organizer
  The 2010 International Chemical Congress of Pacific Basin Societies
- Place of Presentation
  Hawaii Convention Center(Honolulu)
- Year and Date
  2010-12-17
- Data Source
  KAKENHI-PROJECT-22790125
[Presentation] Quantitative glycan analysis during neural differentiation of human mesenchymal stem cells by liquid chromatography/mass spectrometry.2010
- Author(s)
  Hashii, Noritaka
- Organizer
  The 2010 International Chemical Congress of Pacific Basin Societies
- Place of Presentation
  Hawaii Convention Center(Honolulu)
- Year and Date
  2010-12-17
- Data Source
  KAKENHI-PROJECT-22790125
[Presentation] Differential glycan analysis during neural differentiation of human mesenchymal stem cells by quantitative glycan profiling using liquid chromatography/mass spectrometry.2010
- Author(s)
  Hashii, Noritaka
- Organizer
  The 25^<th> International Carbohydrate Symposium(ICS2010)
- Place of Presentation
  幕張メッセ(千葉県)
- Year and Date
  2010-08-05
- Data Source
  KAKENHI-PROJECT-22790125
[Presentation] Identification of cell therapeutic products from stem cells based on glycans.2010
- Author(s)
  Hashii, Noritaka
- Organizer
  第33回日本分子生物学会年会第83回日本生化学会大会合同大会
- Place of Presentation
  神戸ポートアイランド(兵庫県)
- Year and Date
  2010-12-07
- Data Source
  KAKENHI-PROJECT-22790125
[Presentation] 糖鎖プロファイルを指標とした細胞治療薬の特性解析2009
- Author(s)
  橋井則貴
- Organizer
  第8回日本再生医療学会総会
- Place of Presentation
  東京国際フォーラム
- Year and Date
  2009-03-06
- Data Source
  KAKENHI-PROJECT-20790110
[Presentation] 糖鎖を指標とした細胞治療薬の特性解析2009
- Author(s)
  橋井則貴
- Organizer
  日本薬学会第129年会
- Place of Presentation
  国立京都国際会館
- Year and Date
  2009-03-26
- Data Source
  KAKENHI-PROJECT-20790110
[Presentation] 糖鎖プロファイルを指標とした細胞治療薬の特性解析.2009
- Author(s)
  橋井則貴, 川崎ナナ, 篠原聡, 秦艶, 黄笑宇, 伊藤さつき, 山口照英
- Organizer
  第8回日本再生医療学会総会
- Data Source
  KAKENHI-PROJECT-20790110
[Presentation] Role of nuclear sialyl lewis x-conjugated glycoproteins in the proliferation of cancer cell lines2009
- Author(s)
  Daisuke Takakura, Nana Kawasaki, Yan Qin, Xiaoyu Huang, Satsuki Itoh, Noritaka Hashii, Teruhide Yamaguchi
- Organizer
  第32回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜(神奈川)
- Data Source
  KAKENHI-PROJECT-20790110
[Presentation] 糖鎖を指標とした細胞治療薬の特性解析.2009
- Author(s)
  橋井則貴, 川崎ナナ, 篠原聡, 秦艶, 黄笑宇, 伊藤さつき, 山口照英
- Organizer
  日本薬学会第129年会
- Data Source
  KAKENHI-PROJECT-20790110
[Presentation] ヒト間葉系幹細胞のThy-1の糖鎖構造解析.2008
- Author(s)
  伊藤さつき, 川崎ナナ, 篠原聡, 橋井則貴, 山口照英
- Organizer
  第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
- Data Source
  KAKENHI-PROJECT-20790110
[Presentation] d5-フェニルヒドラジンを用いた同位体標識法及び液体クロマトグラフィー/質量分析による糖鎖の定量解析.2008
- Author(s)
  橋井則貴, 川崎ナナ, 中島紫, 伊藤さつき, 山口照英
- Organizer
  第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
- Data Source
  KAKENHI-PROJECT-20790110
[Presentation] 水素／重水素交換質量分析によるヒトインスリンアナログの多量体安定性解析
- Author(s)
  橋井則貴，中澤志織，Joomi Ahn J，廣瀬賢治，川崎ナナ
- Organizer
  Biological Mass Spectrometry Conference
- Place of Presentation
  東京
- Data Source
  KAKENHI-PROJECT-24590069

1. Suzuki Takuo (10415466)

# of Collaborated Projects: 4 results

# of Collaborated Products: 4 results
2. 岡本好海 (40716792)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results

Hashii Noritaka 橋井 則貴

HASHII Noritaka 橋井 則貴

Research Projects

Research Products

Co-Researchers

体内分布や抗原提示に着目したFcRn親和性改変抗体間の違いに関する研究

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

MAPPs assay to elucidate the influences of the change of FcRn affinity on the antigen presentation.

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

HLAの関与する解熱鎮痛薬誘因性重症薬疹の初期発症メカニズムの解明

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Development of analytical method for glycoprotein-glycan interaction by hydrogen/deuterium exchange mass spectrometryPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Basic research on neonatal Fc receptor (FcRn) affinity to establish half-life extension technology of therapeutic antibodies

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Development of technique for higher order structure of glycoprotein by hydrogen/deuterium exchange mass spectrometryPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Development of evaluation methods for comparability of mesenchymal stem cells based on glycansPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Study on neural differentiation of human mesenchymal stem cells based on glycansPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Book] HPLC, GCの測定条件設定テクニックと解析 事例集2016

Author(s)

Total Pages

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[Book] 蛋白質核酸酵素(糖鎖異常の網羅的解析, 臨時増刊号, 糖鎖情報の独自性と普遍性, 53)2008

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[Journal Article] Change in N-Glycosylation of Plasma Proteins in Japanese Semisupercentenarians2015

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[Journal Article] Enrichment of O-GlcNAc-modified peptides using novel thiol-alkyne and thiol-disulfide exchange2015

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[Journal Article] Analysis of the local dynamics of human insulin and a rapid-acting insulin analog by hydrogen/deuterium exchange mass spectrometry2013

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[Journal Article] α1-3/4 fucosylation at Asn 241 ofβ-haptoglobin is a novel marker for colon cancer : A combinatorial approach for development of glycan biomarkers2012

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[Journal Article] Glycomic analysis by liquid chromatography/mass spectrometry2012

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Hashii Noritaka 橋井則貴

HASHII Noritaka 橋井則貴

[Book] HPLC, GCの測定条件設定テクニックと解析　事例集2016