KOBAYASHI YOSHIAKI 小林芳明

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Kobayashi Yoshiaki 小林芳明

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Researcher Number	20907381
Other IDs	https://orcid.org/0000-0002-9585-7255
Affiliation (Current)	2025: 九州大学, 生体防御医学研究所, 助教
Affiliation (based on the past Project Information) *help	2025: 九州大学, 生体防御医学研究所, 助教 2021 – 2022: 東京大学, 大学院理学系研究科(理学部), 特任研究員
Review Section/Research Field	Principal Investigator Basic Section 43010:Molecular biology-related / 0701:Biology at molecular to cellular levels, and related fields
Keywords	Principal Investigator ライブセルイメージング / RNA結合タンパク質 / AGO2 / Argonaute / Argonaute2 / RNAi / siRNA

miRNAによるマウス初期胚の休眠制御機構の解明Principal Investigator
- Principal Investigator
  
  小林芳明
- Project Period (FY)
  2025 – 2028
- Research Category
  
  Grant-in-Aid for Early-Career Scientists
- Review Section
  
  Basic Section 43010:Molecular biology-related
- Research Institution
  Kyushu University
Molecular dynamics of siRNA-AGO complexes in complex cellular environmentsPrincipal Investigator
- Principal Investigator
  
  Kobayashi Yoshiaki
- Project Period (FY)
  2021 – 2022
- Research Category
  
  Grant-in-Aid for Research Activity Start-up
- Review Section
  
  0701:Biology at molecular to cellular levels, and related fields
- Research Institution
  The University of Tokyo

All 2023 2022 2021

All Journal Article Presentation Book

[Book] Clinical Neuroscience_核酸医薬と神経疾患2023
- Author(s)
  小林芳明 , 程久美子
- Total Pages
  736
- Publisher
  中外医学社
- Data Source
  KAKENHI-PROJECT-21K20630
[Book] 核酸医薬・mRNA医薬の製造分析の基礎と基盤技術開発2023
- Author(s)
  小林芳明 , 程久美子 (小比賀聡, 井上貴雄監修)
- Total Pages
  290
- Publisher
  シーエムシー・リサーチ
- Data Source
  KAKENHI-PROJECT-21K20630
[Book] 核酸医薬　本領を発揮する創薬モダリティ2021
- Author(s)
  浅野吉政、小林芳明、程久美子 (編集：横田　隆徳)
- Total Pages
  204
- Publisher
  羊土社
- ISBN
  9784758103985
- Data Source
  KAKENHI-PROJECT-21K20630
[Book] 日本薬学会医薬化学部会機関誌「MEDCHEM NEWS」31-4号「ESSAY特集」2021
- Author(s)
  小林芳明、程久美子 (編集：MEDCHEM NEWS編集委員会)
- Total Pages
  60
- Publisher
  じほうMEDCHEM NEWS
- Data Source
  KAKENHI-PROJECT-21K20630
[Journal Article] SNPD-CRISPR: Single Nucleotide Polymorphism-Distinguishable Repression or Enhancement of a Target Gene Expression by CRISPR System2023
- Author(s)
  Maruyama Shohei、Kusakabe Takashi、Zou Xinyi、Kobayashi Yoshiaki、Asano Yoshimasa、Wang Qingbo S.、Ui-Tei Kumiko
- Journal Title
  
  Methods in Molecular Biology
  
  Volume: 2637 Pages: 49-62
- DOI
  10.1007/978-1-0716-3016-7_4
- ISBN
  9781071630150, 9781071630167
- Data Source
  KAKENHI-PROJECT-21K20630, KAKENHI-PROJECT-22K15158, KAKENHI-PROJECT-21H02465
[Journal Article] siRNAが働く仕組みと医薬品としての研究開発の現状2023
- Author(s)
  小林芳明 , 浅野吉政 , 程久美子
- Journal Title
  
  循環器内科 = Cardioangiology / 循環器内科編集委員会編
  
  Volume: 93 Pages: 346-354
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21K20630
[Journal Article] siRNA Seed Region Is Divided into Two Functionally Different Domains in RNA Interference in Response to 2′-OMe Modifications2022
- Author(s)
  Kobayashi Yoshiaki、Fukuhara Daiki、Akase Dai、Aida Misako、Ui-Tei Kumiko
- Journal Title
  
  ACS Omega
  
  Volume: 7 Issue: 2 Pages: 2398-2410
- DOI
  10.1021/acsomega.1c06455
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-21K20630, KAKENHI-PROJECT-21H02465
[Journal Article] The siRNA Off-Target Effect Is Determined by Base-Pairing Stabilities of Two Different Regions with Opposite Effects2022
- Author(s)
  Kobayashi Yoshiaki、Tian Shen、Ui-Tei Kumiko
- Journal Title
  
  Genes
  
  Volume: 13 Issue: 2 Pages: 319-319
- DOI
  10.3390/genes13020319
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-21K20630, KAKENHI-PROJECT-21H02465
[Journal Article] Knockdown of 15-bp Deletion-Type v-raf Murine Sarcoma Viral Oncogene Homolog B1 mRNA in Pancreatic Ductal Adenocarcinoma Cells Repressed Cell Growth In Vitro and Tumor Volume In Vivo2022
- Author(s)
  Song Jiaxuan、Kobayashi Yoshiaki、Asano Yoshimasa、Sato Atsushi、Taniguchi Hiroaki、Ui-Tei Kumiko
- Journal Title
  
  Cancers
  
  Volume: 14 Issue: 13 Pages: 3162-3162
- DOI
  10.3390/cancers14133162
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-21K20630, KAKENHI-PROJECT-21H02465
[Journal Article] A 2′-modified uridine analog, 2′-O-(methylthiomethoxy)methyl uridine, for siRNA applications2022
- Author(s)
  Fangjie Lyu, Seongjin An, Yoshiaki Kobayashi, Kohei Nomura, Rintaro Baba, Naoko Abe, Haruka Hiraoka, Fumitaka Hashiya, Zhaoma Shu, Kumiko Ui-Tei, Yasuaki Kimura, Hiroshi Abe
- Journal Title
  
  Bioorganic & Medicinal Chemistry Letters
  
  Volume: 74 Pages: 128939-128939
- DOI
  10.1016/j.bmcl.2022.128939
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21K14750, KAKENHI-PROJECT-21K20630, KAKENHI-PROJECT-21H02465, KAKENHI-PROJECT-23K23486
[Journal Article] Thermodynamic stabilities in the siRNA seed and non-seed regions regulate siRNA off-target effect in opposite direction2022
- Author(s)
  Kobayashi Yoshiaki、Tian Shen、Ui-Tei Kumiko
- Journal Title
  
  Research Aspects in Biological Science
  
  Volume: 2 Pages: 117-130
- DOI
  10.9734/bpi/rabs/v2/6153f
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] Development of siRNA for KRAS and BRAF mutations using a cancer cell-derived Xenograft.2022
- Author(s)
  Yoshimasa Asano, Yoshiaki Kobayashi, Sato Atsushi, Hiroaki Taniguchi, Kumiko Ui-Tei
- Organizer
  The Fourth RAS Initiative Symposium
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] Prediction of Structural Change of siRNA by 2'-formamide, a Newly-synthesized Chemical Modification, via Density Functional Theory.2022
- Author(s)
  Seongjin An, Yoshiaki Kobayashi, Kohei Nomura, Yasuaki Kimura, Hiroshi Abe, Dai Akase, Misako Aida, Kumiko Ui-Tei
- Organizer
  CBI学会2022年大会
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] Verification of CRISPR-Cas9 system for sequence-specific regulation of the gut microbiota CRISPR-Cas9システムによる塩基配列特異的な腸内細菌叢制御の検証2022
- Author(s)
  Ryuichi Ono, Yoshiaki Kobayashi, Kumiko Ui-Tei
- Organizer
  第45回日本分子生物学会
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] SNPD-siRNA: an siRNA Specifically Suppresses the Expression of a Target Gene with Single Nucleotide Mutation.2022
- Author(s)
  Kumiko Ui-Tei, Yoshiaki Kobayashi, Yoshimasa Asano, Hiroaki Taniguchi
- Organizer
  The Fourth RAS Initiative Symposium
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] The 2’-formamide modification in the siRNA seed region reduces off-target effects by two different mechanisms 2’-formamide修飾をseed領域にもつsiRNAは2つの異なるメカニズムによってoff-target効果を減弱させる2022
- Author(s)
  Seongjin An, Yoshiaki Kobayashi, Kohei Nomura, Yasuaki Kimura, Hiroshi Abe, Dai Akase, Misako Aida, Kumiko Ui-Tei
- Organizer
  第45回日本分子生物学会
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] Specific knockdown of KRAS mutant gene using SNPD-siRNA repressed cell proliferation of pancreatic cancer cells in vitro and in vivo2022
- Author(s)
  Yoshiaki Kobayashi, Yoshimasa Asano, Atsushi Sato, Hiroaki Taniguchi, Kumiko Ui-Tei
- Organizer
  CBI学会2022年大会
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] Verification of SNPD-siPIK3CA targeting single nucleotide mutation in PIK3CA oncogene in breast cancer-derived cells PIK3CAがん原遺伝子の一塩基多型を識別する小分子干渉RNA (SNPD-siPIK3CA)のがん細胞に対する効果の検証2022
- Author(s)
  Toshinori Ohyama, Yoshiaki Kobayashi, Kumiko Ui-Tei
- Organizer
  第45回日本分子生物学会
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] The siRNA Seed Region Is Composed of Two Functionally Different Domains Which Have Different Effects On RNA Interference and Off-target Activities in Response to 2´-OMe Modifications.2022
- Author(s)
  Yoshiaki Kobayashi, Daiki Fukuhara, Dai Akase, Misako Aida, Kumiko Ui-Tei
- Organizer
  The Fourth RAS Initiative Symposium
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] Development of the CRISPR system to regulate TERT expression utilizing single nucleotide mutations in the promoter region プロモーター領域における一塩基変異を識別してTERT遺伝子の発現を制御するCRISPRシステムの構築2022
- Author(s)
  Hiromu Matsui, Yoshiaki Kobayashi, Kumiko Ui-Tei
- Organizer
  第45回日本分子生物学会
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] Development of siRNA which specifically represses the expression of oncogenic fusion gene がんの原因となる融合遺伝子を特異的に抑制する小分子干渉RNAの開発2022
- Author(s)
  Soto Okubo, Yoshiaki Kobayashi, Kumiko Ui-Tei
- Organizer
  第45回日本分子生物学会
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] 2’-formamide修飾をseed領域にもつsiRNAは塩基の向きを変えることで off-target効果を減弱させる2022
- Author(s)
  安成鎮、小林芳明、野村浩平、木村康明、阿部洋、赤瀬大、相田美砂子、程久美子
- Organizer
  日本核酸医薬学会第7回年会・若手シンポジウム
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] 1塩基の違いを区別して特異的に抑制するsingle-nucleotide polymorphism-distinguishable siRNA (SNPD-siRNA)の開発とその機能解析2022
- Author(s)
  小林芳明，佐藤淳，浅野吉政，谷口博昭，程久美子
- Organizer
  日本核酸医薬学会第7回年会・若手シンポジウム
- Invited
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] The siRNA seed region is divided into two functionally different domains in RNA interference in response to 2´-OMe modifications.2022
- Author(s)
  Yoshiaki Kobayashi, Daiki Fukuhara, Dai Akase, Misako Aida, Kumiko Ui-Tei
- Organizer
  第23回日本RNA学会年会
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] Cell-derived Xenograftモデルを用いたKRASやBRAFの変異部位を対象としたsiRNAの有効性の検証2022
- Author(s)
  浅野吉政、小林芳明、佐藤淳、谷口博昭、程久美子
- Organizer
  日本核酸医薬学会第7回年会
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] Molecular design of single nucleotide polymorphism-distinguishable SNPD-siRNA １塩基変異を区別して遺伝子発現を抑制するSNPD-siRNAの分子設計2022
- Author(s)
  Kumiko Ui-Tei, Yoshiaki Kobayashi, Yoshimasa Asano
- Organizer
  第45回日本分子生物学会
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] PIK3CAがん原遺伝子の一塩基多型を識別する小分子干渉RNA (SNPD-siRNA)の開発2022
- Author(s)
  大山隼礼、小林芳明、程久美子
- Organizer
  日本核酸医薬学会第7回年会
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] PIK3CAがん原遺伝子の一塩基変異を識別して抑制する小分子干渉RNA(SNPD-siRNA)の有効性の検証2021
- Author(s)
  大山隼礼, 小林芳明, 程久美子
- Organizer
  第44回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] トリプレットリピート病の原因遺伝子における一塩基多型を識別する小分子干渉RNA（SNPD-siRNA）の構築とマカド・ジョセフ病由来細胞における検証2021
- Author(s)
  鈴木ゆりあ, 小林芳明, 程久美子
- Organizer
  第44回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] 2´-OMe修飾したsiRNAのseed領域は2つの機能ドメインをもつ2021
- Author(s)
  小林芳明, 田申, 福原大輝, 赤瀬大, 相田美砂子, 程久美子
- Organizer
  第44回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-21K20630
[Presentation] BaseとSugar修飾を利用したsiRNAのoff-target効果の回避2021
- Author(s)
  Seongjin An, Yoshiaki Kobayashi, Kohei Nomura, Akase Dai, Yasuaki Kimura, Hiroshi Abe, Misako Aida, Kumiko Ui-Tei
- Organizer
  第44回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-21K20630

1. 平岡陽花

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

KOBAYASHI YOSHIAKI 小林 芳明

Kobayashi Yoshiaki 小林 芳明

Research Projects

Research Products

Co-Researchers

miRNAによるマウス初期胚の休眠制御機構の解明Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Molecular dynamics of siRNA-AGO complexes in complex cellular environmentsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

[Book] Clinical Neuroscience_核酸医薬と神経疾患2023

Author(s)

Total Pages

Publisher

Data Source

[Book] 核酸医薬・mRNA医薬の製造分析の基礎と基盤技術開発2023

Author(s)

Total Pages

Publisher

Data Source

[Book] 核酸医薬 本領を発揮する創薬モダリティ2021

Author(s)

Total Pages

Publisher

ISBN

Data Source

[Book] 日本薬学会医薬化学部会機関誌「MEDCHEM NEWS」31-4号「ESSAY特集」2021

Author(s)

Total Pages

Publisher

Data Source

[Journal Article] SNPD-CRISPR: Single Nucleotide Polymorphism-Distinguishable Repression or Enhancement of a Target Gene Expression by CRISPR System2023

Author(s)

Journal Title

DOI

ISBN

Data Source

[Journal Article] siRNAが働く仕組みと医薬品としての研究開発の現状2023

Author(s)

Journal Title

Data Source

[Journal Article] siRNA Seed Region Is Divided into Two Functionally Different Domains in RNA Interference in Response to 2′-OMe Modifications2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] The siRNA Off-Target Effect Is Determined by Base-Pairing Stabilities of Two Different Regions with Opposite Effects2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Knockdown of 15-bp Deletion-Type v-raf Murine Sarcoma Viral Oncogene Homolog B1 mRNA in Pancreatic Ductal Adenocarcinoma Cells Repressed Cell Growth In Vitro and Tumor Volume In Vivo2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] A 2′-modified uridine analog, 2′-O-(methylthiomethoxy)methyl uridine, for siRNA applications2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Thermodynamic stabilities in the siRNA seed and non-seed regions regulate siRNA off-target effect in opposite direction2022

Author(s)

Journal Title

DOI

Data Source

[Presentation] Development of siRNA for KRAS and BRAF mutations using a cancer cell-derived Xenograft.2022

Author(s)

Organizer

Data Source

[Presentation] Prediction of Structural Change of siRNA by 2'-formamide, a Newly-synthesized Chemical Modification, via Density Functional Theory.2022

Author(s)

Organizer

KOBAYASHI YOSHIAKI 小林芳明

Kobayashi Yoshiaki 小林芳明

[Book] 核酸医薬　本領を発揮する創薬モダリティ2021