YOSHIDA Toru 吉田徹

Researcher Number	30724546
Other IDs
Affiliation (Current)	2025: 日本女子大学, 理学部, 助教
Affiliation (based on the past Project Information) *help	2024: 日本女子大学, 理学部, 助教 2020 – 2022: 日本女子大学, 理学部, 助教 2019: 京都産業大学, 総合生命科学部, 研究員 2017 – 2018: 京都産業大学, 総合生命科学部, 研究助教
Review Section/Research Field	Principal Investigator Basic Section 37020:Chemistry and chemical methodology of biomolecules-related / Basic Section 43040:Biophysics-related / Functional biochemistry Except Principal Investigator Basic Section 43040:Biophysics-related / Basic Section 38030:Applied biochemistry-related / Applied biochemistry / General medical chemistry
Keywords	Principal Investigator クライオ電子顕微鏡 / トモグラフィー / セルロース / Cryo-EM / ミニセル / Cryo-ET / バクテリアセルロース / 結晶構造解析 / 翻訳後修飾 / X線結晶構造解析 / 基質特異性 / ADPリボシル化 … More Except Principal Investigator … More クライオ電子顕微鏡 / タンパク質膜透過 / 二成分毒素 / DyP / ADPリボシル化 / Komagataeibacter xylinus / セルロース生産菌 / エンカプスリン / １分子測定 / トキシン膜透過システム / 膜孔形成毒素 / フォールディング / ADPリボシルトランスフェラーゼ / 複合体構造解析 / トランスロコン / 膜孔 / アンフォールド / 膜結合 / イオタ毒素 / 超分子複合体 / 単粒子解析 / ディフィシル菌 / ウェルシュ菌 / ＤｙＰ型ペルオキシダーゼ / タンパク質機能 / 立体構造のトポロジー / タンパク質工学 / 収束進化 / 分子進化 / DyP型ペルオキシダーゼ / 構造酵素学 / 複合体 / Rac1 / CPILE-a / 基質タンパク質認識 / 細菌毒素 / アクチン / 修飾アミノ酸認識機構 / 基質タンパク質認識機構 / Ia / X線結晶構造解析 / タンパク質複合体 / Cdc42 / RhoA / C3 / 基質特異性 Less

Toxin membrane translocation system approached by liposome single particle analysis
- Principal Investigator
  
  津下英明
- Project Period (FY)
  2024 – 2026
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 43040:Biophysics-related
- Research Institution
  Kyoto Sangyo University
酢酸菌によるセルロース繊維の結晶系制御システムの解明Principal Investigator
- Principal Investigator
  
  吉田徹
- Project Period (FY)
  2024 – 2026
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 37020:Chemistry and chemical methodology of biomolecules-related
- Research Institution
  Japan Women's University
Role of encapsulin and DyP from cellulose high-producing bacateria
- Principal Investigator
  
  菅野靖史
- Project Period (FY)
  2024 – 2026
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 38030:Applied biochemistry-related
- Research Institution
  Japan Women's University
A mechanism by which acetic acid bacteria form a single cellulose fiber with multiple cellulose synthase complexes on the cell membrane.Principal Investigator
- Principal Investigator
  
  YOSHIDA Toru
- Project Period (FY)
  2020 – 2022
- Research Category
  
  Grant-in-Aid for Early-Career Scientists
- Review Section
  
  Basic Section 43040:Biophysics-related
- Research Institution
  Japan Women's University
Structural and Functional Study of Binary Toxin Translocon by Cryo-EM and X-ray Crystallography
- Principal Investigator
  
  TSUGE Hideaki
- Project Period (FY)
  2018 – 2019
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 43040:Biophysics-related
- Research Institution
  Kyoto Sangyo University
Two kinds of specificities essential for mono-ADP-ribosyltransferase reaction.Principal Investigator
- Principal Investigator
  
  YOSHIDA Toru
- Project Period (FY)
  2017 – 2019
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Functional biochemistry
- Research Institution
  Kyoto Sangyo University
Rho GTPase Recognition Mechanism of ADP-ribosylating C3 exoenzyme
- Principal Investigator
  
  TSUGE Hideaki
- Project Period (FY)
  2015 – 2017
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  General medical chemistry
- Research Institution
  Kyoto Sangyo University
Rational design of DyP type peroxidase based on the topology
- Principal Investigator
  
  SUGANO Yasushi
- Project Period (FY)
  2014 – 2016
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Applied biochemistry
- Research Institution
  Japan Women's University

All 2023 2022 2020 2019 2018 2017 2016 2015

All Journal Article Presentation

[Journal Article] Unexpected diversity of dye-decolorizing peroxidases2023
- Author(s)
  Yoshida Toru、Sugano Yasushi
- Journal Title
  
  Biochemistry and Biophysics Reports
  
  Volume: 33 Pages: 101401-101401
- DOI
  10.1016/j.bbrep.2022.101401
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K15763
[Journal Article] Cryo-EM structures of the translocational binary toxin complex CDTa-bound CDTb-pore from Clostridioides difficile2022
- Author(s)
  Kawamoto Akihiro、Yamada Tomohito、Yoshida Toru、Sato Yusui、Kato Takayuki、Tsuge Hideaki
- Journal Title
  
  Nature Communications
  
  Volume: 13 Issue: 1 Pages: 6119-6119
- DOI
  10.1038/s41467-022-33888-4
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21J13410, KAKENHI-PROJECT-20K15763, KAKENHI-PROJECT-21H02452
[Journal Article] Cryo-EM structures reveal translocational unfolding in the clostridial binary iota toxin complex2020
- Author(s)
  Yamada Tomohito、Yoshida Toru、Kawamoto Akihiro、Mitsuoka Kaoru、Iwasaki Kenji、Tsuge Hideaki
- Journal Title
  
  Nature Structural & Molecular Biology
  
  Volume: 27 Issue: 3 Pages: 288-296
- DOI
  10.1038/s41594-020-0388-6
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K06170, KAKENHI-PROJECT-18K14639
[Journal Article] Substrate N2 atom recognition mechanism in pierisin family DNA-targeting, guanine-specific ADP-ribosyltransferase ScARP2018
- Author(s)
  Yoshida Toru、Tsuge Hideaki
- Journal Title
  
  Journal of Biological Chemistry
  
  Volume: 293 Issue: 36 Pages: 13768-13774
- DOI
  10.1074/jbc.ac118.004412
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K15095, KAKENHI-PROJECT-18K06170
[Journal Article] Crystal structure and structure-based mutagenesis of actin-specific ADP-ribosylating toxin CPILE-a as novel enterotoxin.2017
- Author(s)
  Toniti, W., Yoshida, T., Tsurumura, T., Irikura, D., Monma, C., Kamata, Y., and Tsuge, H.
- Journal Title
  
  PLoS ONE
  
  Volume: 12 Issue: 2 Pages: e0171278-e0171278
- DOI
  10.1371/journal.pone.0171278
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-16J09051, KAKENHI-PROJECT-15K08289
[Journal Article] Comparative Studies of Actin- and Rho-Specific ADP-Ribosylating Toxins: Insight from Structural Biology2017
- Author(s)
  Tsuge H, Tsurumura T, Toda A, Murata H, Toniti W, Yoshida T.
- Journal Title
  
  Curr Top Microbiol Immunol.
  
  Volume: 399 Pages: 69-86
- DOI
  10.1007/82_2016_23
- ISBN
  9783319500461, 9783319500478
- Peer Reviewed / Acknowledgement Compliant
- Data Source
  KAKENHI-PROJECT-15K08289
[Journal Article] Involvement of the Arg566 residue of Aeromonas sobria serine protease in substrate specificity.2017
- Author(s)
  Kobayashi H, Otsubo T, Teraoka F, Ikeda K, Seike S, Takahashi E, Okamoto K, Yoshida T, Tsuge H, Yamanaka H
- Journal Title
  
  PLoS One
  
  Volume: - Issue: 10 Pages: e0186392-e0186392
- DOI
  10.1371/journal.pone.0186392
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-15K08289, KAKENHI-PROJECT-16K18926
[Journal Article] Anabaena sp. DyP-type peroxidase is a tetramer consisting of two asymmetirc dimers2016
- Author(s)
  T. Yoshida, H.J.O. Ogola, Y. Amano, T. Hisabori, H. Ashida, Y. Sawa, H. Tsuge, Y. Sugano
- Journal Title
  
  Proteins
  
  Volume: 84 Issue: 1 Pages: 31-42
- DOI
  10.1002/prot.24952
- Peer Reviewed / Acknowledgement Compliant
- Data Source
  KAKENHI-PROJECT-26450132
[Journal Article] Conformational plasticity is crucial for C3-RhoA complex formation by ARTT-loop2015
- Author(s)
  Tsuge H, Yoshida T, Tsurumura T
- Journal Title
  
  Pathog Dis
  
  Volume: 73 Issue: 9 Pages: ftv094-ftv094
- DOI
  10.1093/femspd/ftv094
- Peer Reviewed / Acknowledgement Compliant
- Data Source
  KAKENHI-PROJECT-15K08289
[Journal Article] Rho GTPase Recognition by C3 Exoenzyme Based on C3-RhoA Complex Structure2015
- Author(s)
  Toda A, Tsurumura T, Yoshida T, Tsumori Y, Tsuge H.
- Journal Title
  
  J Biol Chem
  
  Volume: 290 Issue: 32 Pages: 19423-32
- DOI
  10.1074/jbc.m115.653220
- Peer Reviewed / Acknowledgement Compliant
- Data Source
  KAKENHI-PROJECT-15K08289
[Presentation] Clostridium perfringens由来二成分毒素CPILEbの膜貫通孔は、セリンで形成された最狭窄部位を持つ2022
- Author(s)
  吉田徹、内田悠斗、山田等仁、津下英明
- Organizer
  第68回トキシンシンポジウム
- Data Source
  KAKENHI-PROJECT-20K15763
[Presentation] DNAを基質とするADPリボシル基転移酵素の基質認識機構2019
- Author(s)
  吉田徹、津下英明
- Organizer
  酵素・補酵素研究会2019
- Data Source
  KAKENHI-PROJECT-17K15095
[Presentation] Cryo-EM structure of clostridial binary toxin translocon Ib-pore2019
- Author(s)
  Yamada T, Yoshida T, Kawamoto A, Mitsuoka K, Iwasaki K, and Tsuge H
- Organizer
  European Workshops on Bacterial Protein Toxins - ETOX19
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K06170
[Presentation] Cryo-EM structure of clostridial binary toxin translocation channel Ib-pore2019
- Author(s)
  山田等仁、吉田徹、川本暁大、光岡薫、岩崎憲司、津下英明
- Organizer
  第57回日本生物物理学会
- Data Source
  KAKENHI-PROJECT-18K06170
[Presentation] Substrate recognition and reaction mechanism of DNA-targeting guanine-specific ADP-ribosyltransferase2018
- Author(s)
  Yoshida Toru、Tsuge Hideaki
- Organizer
  International Symposium on “Proteins; from the Cradle to the Grave”
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K15095
[Presentation] Membrane transport and ADP-ribosylation mechanism of binary toxin C. perfringens iota toxin2018
- Author(s)
  Yoshida　T, Yamada T, and Tsuge H
- Organizer
  International Symposium on “Proteins; from the Cradle to the Grave”
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K06170
[Presentation] Substrate recognition and reaction mechanism of DNA-targeting guanine-specific ADP-ribosyltransferase2018
- Author(s)
  Yoshida　T, and Tsuge H
- Organizer
  International Symposium on “Proteins; from the Cradle to the Grave”
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K06170
[Presentation] Substrate and target atom recognition mechanism of guanine-specific ADP-ribosyltransferase2018
- Author(s)
  Yoshida Toru、Tsuge Hideaki
- Organizer
  第18回日本蛋白質科学会年会
- Data Source
  KAKENHI-PROJECT-17K15095
[Presentation] Cryo-EM single particle analysis of C. perfringens binary toxin2018
- Author(s)
  Yamada T, Yoshida T, and Tsuge H
- Organizer
  International Symposium on “Proteins; from the Cradle to the Grave”
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K06170
[Presentation] Structure determination of Clostridium perfringens iota-like enterotoxin by X-ray crystallography2017
- Author(s)
  Toru Yoshida, Hidetomo Kobayashi, Hiroyasu Yamanaka, Hideaki Tsuge
- Organizer
  ETOX18
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K08289
[Presentation] Crystal structure and structure-based mutagenesis of actin-specific ADP-ribosylating toxin CPILE-a as novel enterotoxin2017
- Author(s)
  Waraphan Toniti, Toru Yoshida, Toshiharu Tsurumura, Daisuke Irikura, Chie Monma, Youichi Kamata, Hideaki Tsuge
- Organizer
  ETOX18
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K08289
[Presentation] Characterization of Actin ADP-ribosyltransferase from Clostridium perfringens iota-like enterotoxin2016
- Author(s)
  Waraphan Toniti, Toru Yoshida, Toshiharu Tsurumura, Daisuke Irikura, Chie Monma, Yoichi Kamata, and Hideaki Tsuge
- Organizer
  ICC05-AEM2016
- Place of Presentation
  宇奈月国際会館（富山）
- Year and Date
  2016-09-08
- Data Source
  KAKENHI-PROJECT-15K08289
[Presentation] ADP-ribosylation of RhoA by C3 exoenzyme.2016
- Author(s)
  Toru Yoshida, and Hideaki Tsuge
- Organizer
  ICC05-AEM2016
- Place of Presentation
  宇奈月国際会館（富山）
- Year and Date
  2016-09-08
- Data Source
  KAKENHI-PROJECT-15K08289
[Presentation] C3 exoenzymeとRhoA複合体の結晶構造解析に基づく基質特異性2016
- Author(s)
  津下英明、戸田暁之、鶴村俊治、吉田徹
- Organizer
  日本分子生物学会
- Place of Presentation
  パシフィコ横浜（横浜）
- Year and Date
  2016-12-02
- Data Source
  KAKENHI-PROJECT-15K08289
[Presentation] An improved classification of DyP-type peroxidase family accelerates the research of the family2015
- Author(s)
  Y. Sugano, T. Yoshida
- Organizer
  FEMS 2015
- Place of Presentation
  マーストリヒト　オランダ
- Year and Date
  2015-06-07
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-26450132
[Presentation] Structural basis of ADP-ribosyltransferase C3 exoenzyme with RhoA complex2015
- Author(s)
  戸田暁之、鶴村俊治、吉田徹、津守耶良、津下英明
- Organizer
  日本生物物理学会
- Place of Presentation
  金沢大学（石川県、金沢市）
- Year and Date
  2015-09-13
- Data Source
  KAKENHI-PROJECT-15K08289

1. TSUGE Hideaki (40299342)

# of Collaborated Projects: 5 results

# of Collaborated Products: 17 results
2. SUGANO Yasushi (90282855)

# of Collaborated Projects: 3 results

# of Collaborated Products: 2 results
3. 杉田征彦 (00734469)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
4. TONITI WARAPHAN

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results

YOSHIDA Toru 吉田 徹

Research Projects

Research Products

Co-Researchers

Toxin membrane translocation system approached by liposome single particle analysis

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

酢酸菌によるセルロース繊維の結晶系制御システムの解明Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Role of encapsulin and DyP from cellulose high-producing bacateria

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

A mechanism by which acetic acid bacteria form a single cellulose fiber with multiple cellulose synthase complexes on the cell membrane.Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Structural and Functional Study of Binary Toxin Translocon by Cryo-EM and X-ray Crystallography

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Two kinds of specificities essential for mono-ADP-ribosyltransferase reaction.Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Rho GTPase Recognition Mechanism of ADP-ribosylating C3 exoenzyme

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Rational design of DyP type peroxidase based on the topology

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Journal Article] Unexpected diversity of dye-decolorizing peroxidases2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Cryo-EM structures of the translocational binary toxin complex CDTa-bound CDTb-pore from Clostridioides difficile2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Cryo-EM structures reveal translocational unfolding in the clostridial binary iota toxin complex2020

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Substrate N2 atom recognition mechanism in pierisin family DNA-targeting, guanine-specific ADP-ribosyltransferase ScARP2018

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Crystal structure and structure-based mutagenesis of actin-specific ADP-ribosylating toxin CPILE-a as novel enterotoxin.2017

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Comparative Studies of Actin- and Rho-Specific ADP-Ribosylating Toxins: Insight from Structural Biology2017

Author(s)

Journal Title

YOSHIDA Toru 吉田徹