Shihoya Wataru 志甫谷渉

Researcher Number	30809421
Other IDs
Affiliation (Current)	2025: 慶應義塾大学, 医学部(信濃町), 准教授 2025: 東京大学, 大学院理学系研究科(理学部), 助教
Affiliation (based on the past Project Information) *help	2020 – 2025: 東京大学, 大学院理学系研究科(理学部), 助教 2018 – 2019: 東京大学, 大学院理学系研究科(理学部), 特別研究員
Review Section/Research Field	Principal Investigator Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related / Basic Section 43020:Structural biochemistry-related / Medium-sized Section 47:Pharmaceutical sciences and related fields / Complex systems Except Principal Investigator Complex systems
Keywords	Principal Investigator GPCR / クライオ電子顕微鏡法 / 構造生物学 / クライオ電子顕微鏡 / X線結晶構造解析 / ロドプシン / Cryo-EM / 薬剤 / リゾリン脂質受容体 / ヘリオロドプシン … More / X線自由電子レーザー / Ｘ線自由電子レーザー / Gタンパク質共役受容体 / 単粒子解析 / エンドセリン受容体 / エンドセリン / G蛋白質 / PACAP … More Except Principal Investigator チャネルロドプシン / X線結晶構造解析 / フォトクロミックタンパク質 / 構造ダイナミクス / ロドプシン / クライオ電子顕微鏡単粒子解析 / 時分割X線結晶構造解析 / X線自由電子レーザー / 膜タンパク質 / 光遺伝学 Less

クライオ電子顕微鏡法を用いたGPCR標的型機能性抗体の開発と応用Principal Investigator
- Principal Investigator
  
  志甫谷渉
- Project Period (FY)
  2025 – 2027
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
- Research Institution
  The University of Tokyo
GPCR drug discovery 2.0 targeting lysophospholipid-activated GPCRsPrincipal Investigator
- Principal Investigator
  
  志甫谷渉
- Project Period (FY)
  2022 – 2024
- Research Category
  
  Grant-in-Aid for Challenging Research (Exploratory)
- Review Section
  
  Medium-sized Section 47:Pharmaceutical sciences and related fields
- Research Institution
  The University of Tokyo
Structural analysis and drug development of GPCRsPrincipal Investigator
- Principal Investigator
  
  志甫谷渉
- Project Period (FY)
  2022 – 2025
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
- Research Institution
  The University of Tokyo
Structural and funtinal analysis of non-cannonical rhodopsinsPrincipal Investigator
- Principal Investigator
  
  Shihoya Wataru
- Project Period (FY)
  2020 – 2022
- Research Category
  
  Grant-in-Aid for Early-Career Scientists
- Review Section
  
  Basic Section 43020:Structural biochemistry-related
- Research Institution
  The University of Tokyo
TR-SFX study of non-canonical rhodopsinPrincipal Investigator
- Principal Investigator
  
  志甫谷渉
- Project Period (FY)
  2020 – 2021
- Research Category
  
  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
- Review Section
  
  Complex systems
- Research Institution
  The University of Tokyo
Time-resolved XFEL crystallography and rational engineering of light-driven proteins
- Principal Investigator
  
  Iwata So
- Project Period (FY)
  2019 – 2023
- Research Category
  
  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
- Review Section
  
  Complex systems
- Research Institution
  Kyoto University
Structural analysis of endothelin receptors in complex with G-proteinsPrincipal Investigator
- Principal Investigator
  
  Shihoya Wataru
- Project Period (FY)
  2018 – 2019
- Research Category
  
  Grant-in-Aid for Early-Career Scientists
- Review Section
  
  Basic Section 43020:Structural biochemistry-related
- Research Institution
  The University of Tokyo

All 2024 2023 2022 2021 2020 2019 2018

All Journal Article

[Journal Article] Cryo-EM advances in GPCR structure determination2024
- Author(s)
  Shihoya Wataru、Iwama Aika、Sano Fumiya K、Nureki Osamu
- Journal Title
  
  The Journal of Biochemistry
  
  Volume: 未定 Issue: 1 Pages: 1-10
- DOI
  10.1093/jb/mvae029
- Data Source
  KAKENHI-PLANNED-19H05777
[Journal Article] Molecular insights into atypical modes of β-arrestin interaction with seven transmembrane receptors2024
- Author(s)
  Jagannath Maharana , Fumiya K Sano , Parishmita Sarma , Manish K Yadav, Longhan Duan , Tomasz M Stepniewski , Madhu Chaturvedi, Ashutosh Ranjan, Vinay Singh , Sayantan Saha , Gargi Mahajan , Mohamed Chami , Wataru Shihoya , Jana Selent , Ka Young Chung , Ramanuj Banerjee , Osamu Nureki , Arun K Shukla
- Journal Title
  
  Science
  
  Volume: 383 Issue: 6678 Pages: 101-108
- DOI
  10.1126/science.adj3347
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21H05037, KAKENHI-PLANNED-19H05777
[Journal Article] Optimizing cryo-EM structural analysis of Gi-coupling receptors via engineered Gt and Nb35 application2024
- Author(s)
  Hidetaka S Oshima, Fumiya K Sano, Hiroaki Akasaka, Aika Iwama, Wataru Shihoya, Osamu Nureki
- Journal Title
  
  Biochem Biophys Res Commun
  
  Volume: 693 Pages: 149361-149361
- DOI
  10.1016/j.bbrc.2023.149361
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-21H05037, KAKENHI-PLANNED-19H05777
[Journal Article] Structural basis for lysophosphatidylserine recognition by GPR342024
- Author(s)
  Izume Tamaki、Kawahara Ryo、Uwamizu Akiharu、Chen Luying、Yaginuma Shun、Omi Jumpei、Kawana Hiroki、Hou Fengjue、Sano Fumiya K.、Tanaka Tatsuki、Kobayashi Kazuhiro、Okamoto Hiroyuki H.、Kise Yoshiaki、Ohwada Tomohiko、Aoki Junken、Shihoya Wataru、Nureki Osamu
- Journal Title
  
  Nature Communications
  
  Volume: 15 Issue: 1 Pages: 902-902
- DOI
  10.1038/s41467-024-45046-z
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-22K19263, KAKENHI-PROJECT-22K19371, KAKENHI-PROJECT-22K15273, KAKENHI-PROJECT-22KJ0530, KAKENHI-PROJECT-22KJ0577, KAKENHI-PROJECT-22KJ0604, KAKENHI-PROJECT-21H05037, KAKENHI-PROJECT-23K23819, KAKENHI-PLANNED-19H05777
[Journal Article] Phototrophy by Antenna-Containing Rhodopsin Pumps in Aquatic Environments2023
- Author(s)
  Chazan, Das, Fujiwara, Murakoshi, Rozenberg, Molina-Marquez, Sano, Tanaka, Gomez-Villegas, Larom, Pushkarev, Malakar, Hasegawa, Tsukamoto, Ishizuka, Konno, Nagata, Mizuno, Katayama, Abe-Yoshizumi, Ruhman, Inoue, Kandori, Leon, Shihoya, Yoshizawa, Sheves, Nureki, Beja
- Journal Title
  
  Nature
  
  Volume: 615 Issue: 7952 Pages: 535-540
- DOI
  10.1038/s41586-023-05774-6
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PLANNED-20H05758, KAKENHI-PROJECT-22KJ3197, KAKENHI-PROJECT-22KJ1110, KAKENHI-PROJECT-20K21383, KAKENHI-PROJECT-20K15728, KAKENHI-PROJECT-21H05037, KAKENHI-PROJECT-21H04969, KAKENHI-PLANNED-19H05777, KAKENHI-PROJECT-23K21092, KAKENHI-PROJECT-22H00557
[Journal Article] Cryo-EM structure of the endothelin-1-ETB-Gi complex2023
- Author(s)
  Sano Fumiya K、Akasaka Hiroaki、Shihoya Wataru、Nureki Osamu
- Journal Title
  
  eLife
  
  Volume: 12 Pages: 1-14
- DOI
  10.7554/elife.85821
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-22K19371, KAKENHI-PLANNED-19H05777
[Journal Article] Structural Insights into Endothelin Receptor Signaling2023
- Author(s)
  Shihoya, Wataru, Sano, Fumiya K, Nureki, Osamu,
- Journal Title
  
  J Biochem,
  
  Volume: 29 Issue: 4 Pages: 317-325
- DOI
  10.1093/jb/mvad055
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-21H05037, KAKENHI-PLANNED-19H05777
[Journal Article] Endogenous ligand recognition and structural transition of a human PTH receptor2022
- Author(s)
  Kobayashi Kazuhiro、Kawakami Kouki、Kusakizako Tsukasa、Miyauchi Hirotake、Tomita Atsuhiro、Kobayashi Kan、Shihoya Wataru、Yamashita Keitaro、Nishizawa Tomohiro、Kato Hideaki E.、Inoue Asuka、Nureki Osamu
- Journal Title
  
  Molecular Cell
  
  Volume: 82 Issue: 18 Pages: 3468-3483
- DOI
  10.1016/j.molcel.2022.07.003
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-22K15072, KAKENHI-PLANNED-21H05113, KAKENHI-PROJECT-21H04791, KAKENHI-PROJECT-20J21820, KAKENHI-PROJECT-20K15754, KAKENHI-PROJECT-21H05037, KAKENHI-PLANNED-19H05777
[Journal Article] Cryo-EM structures of the β3 adrenergic receptor bound to solabegron and isoproterenol2022
- Author(s)
  Nureki Ikko、Kobayashi Kazuhiro、Tanaka Tatsuki、Demura Kanae、Inoue Asuka、Shihoya Wataru、Nureki Osamu
- Journal Title
  
  Biochemical and Biophysical Research Communications
  
  Volume: 611 Pages: 158-164
- DOI
  10.1016/j.bbrc.2022.04.065
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PLANNED-21H05113, KAKENHI-PROJECT-21H04791, KAKENHI-PROJECT-21H05037, KAKENHI-PROJECT-23K24014, KAKENHI-PLANNED-19H05777
[Journal Article] Pharmacophore-guided Virtual Screening to Identify New β3 -adrenergic Receptor Agonists2022
- Author(s)
  Ujiantari NSO, 他 3 名, Nureki O(5 番目), Hutchinson DS, Schuster D
- Journal Title
  
  Mol. Inform.
  
  Volume: 41 Issue: 7 Pages: 2100223-2100223
- DOI
  10.1002/minf.202100223
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21H05037, KAKENHI-PLANNED-19H05777
[Journal Article] Structure of the active Gi-coupled human lysophosphatidic acid receptor 1 complexed with a potent agonist2022
- Author(s)
  Akasaka Hiroaki、Tanaka Tatsuki、Sano Fumiya K.、Matsuzaki Yuma、Shihoya Wataru、Nureki Osamu
- Journal Title
  
  Nature Communications
  
  Volume: 13 Issue: 1 Pages: 1-12
- DOI
  10.1038/s41467-022-33121-2
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-22K19371, KAKENHI-PROJECT-21H05037, KAKENHI-PROJECT-23K24014, KAKENHI-PLANNED-19H05777
[Journal Article] Cryo-EM structure of the β3-adrenergic receptor reveals the molecular basis of subtype selectivity.2021
- Author(s)
  Nagiri, C., Kobayashi, K., Tomita, A., Kato, M., Kobayashi, K., Yamashita, K., Nishizawa, T., Inoue, A., Shihoya, W. and Nureki, O.
- Journal Title
  
  Mol. Cell
  
  Volume: 81 Issue: 15 Pages: 3205-3215
- DOI
  10.1016/j.molcel.2021.06.024
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-16H06294, KAKENHI-PUBLICLY-20H05437, KAKENHI-PROJECT-20J13299, KAKENHI-PROJECT-20K15728, KAKENHI-PROJECT-21H05037
[Journal Article] Cryo-EM structure of the human MT1-Gi signaling complex.2021
- Author(s)
  Okamoto, H. H., Miyauchi, H., Inoue, A., Raimondi, F., Tsujimoto, H., Kusakizako, T., Shihoya, W., Yamashita, K., Suno, R., Nomura, N., Kobayashi, T., Iwata, S., Nishizawa, T. and Nureki, O.
- Journal Title
  
  Nat. Struct. Mol. Biol.
  
  Volume: 28 Issue: 8 Pages: 694-701
- DOI
  10.1038/s41594-021-00634-1
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16H06294, KAKENHI-PLANNED-21H05112, KAKENHI-PROJECT-19H00923, KAKENHI-PROJECT-19H03428, KAKENHI-PROJECT-21K19360, KAKENHI-PROJECT-20K15754, KAKENHI-PROJECT-22KJ0604, KAKENHI-PROJECT-21H05037, KAKENHI-PLANNED-19H05777
[Journal Article] Crystal Structure of Schizorhodopsin Reveals Mechanism of Inward Proton Pumping2021
- Author(s)
  Akimitsu Higuchi, Wataru Shihoya, Masae Konno, Tatsuya Ikuta, Hideki Kandori, Keiichi Inoue, Osamu Nureki
- Journal Title
  
  Proceedings of the National Academy of Sciences of the United States of America
  
  Volume: 118 Issue: 14 Pages: 1-9
- DOI
  10.1073/pnas.2016328118
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20K21383, KAKENHI-PROJECT-20K15728, KAKENHI-PUBLICLY-20H05437, KAKENHI-PLANNED-20H05758, KAKENHI-PROJECT-17H03007, KAKENHI-PROJECT-20K21251
[Journal Article] Structural insights into the mechanism of rhodopsin phosphodiesterase2020
- Author(s)
  Ikuta T, Shihoya W, Sugiura M, Yoshida K, Watari M, Tokano T, Yamashita K, Katayama K, Tsunoda SP, Uchihashi T, Kandori H and Nureki O.
- Journal Title
  
  Nat. Commun.
  
  Volume: 11 Issue: 1 Pages: 5605-5605
- DOI
  10.1038/s41467-020-19376-7
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K06109, KAKENHI-PROJECT-20K21251, KAKENHI-PROJECT-20K15728, KAKENHI-PUBLICLY-19H04959, KAKENHI-PUBLICLY-19H05389, KAKENHI-PUBLICLY-20H04669, KAKENHI-PUBLICLY-20H05437, KAKENHI-PROJECT-18H03986, KAKENHI-PROJECT-18H01837, KAKENHI-PROJECT-19J13316, KAKENHI-PROJECT-16H06294
[Journal Article] Cryo-EM structure of the human PAC1 receptor coupled to an engineered heterotrimeric G protein2020
- Author(s)
  Kobayashi Kazuhiro、Shihoya Wataru、Nishizawa Tomohiro、Kadji Francois Marie Ngako、Aoki Junken、Inoue Asuka、Nureki Osamu
- Journal Title
  
  Nature Structural & Molecular Biology
  
  Volume: 27 Issue: 3 Pages: 274-280
- DOI
  10.1038/s41594-020-0386-8
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-17H05000, KAKENHI-PROJECT-17J30010, KAKENHI-PROJECT-17K08264, KAKENHI-PROJECT-18K14634, KAKENHI-PROJECT-20J21820
[Journal Article] Crystal structure of human endothelin ETB receptor in complex with peptide inverse agonist IRL25002019
- Author(s)
  Nagiri Chisae、Shihoya Wataru、Inoue Asuka、Kadji Francois Marie Ngako、Aoki Junken、Nureki Osamu
- Journal Title
  
  Communications Biology
  
  Volume: 2 Issue: 1 Pages: 1-11
- DOI
  10.1038/s42003-019-0482-7
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17J30010, KAKENHI-PROJECT-17K08264, KAKENHI-PROJECT-18K14634, KAKENHI-PROJECT-16H06294
[Journal Article] Crystal structures of human ETB receptor provide mechanistic insight into receptor activation and partial activation2018
- Author(s)
  Shihoya Wataru、Izume Tamaki、Inoue Asuka、Yamashita Keitaro、Kadji Francois Marie Ngako、Hirata Kunio、Aoki Junken、Nishizawa Tomohiro、Nureki Osamu
- Journal Title
  
  Nature Communications
  
  Volume: 9 Issue: 1 Pages: 1-11
- DOI
  10.1038/s41467-018-07094-0
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-17K08264, KAKENHI-PROJECT-18K14634, KAKENHI-PROJECT-17J30010, KAKENHI-PROJECT-16H06294, KAKENHI-PROJECT-17H05000

1. Iwata So (60452330)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
2. 野村紀通 (10314246)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
3. 近藤美欧 (20619168)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
4. 草木迫司 (80815316)

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
5. 山下恵太郎 (20721690)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
6. INOUE Asuka

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
7. NISHIZAWA Tomohiro

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
8. INOUE Keiichi

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
9. 角田聡

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
10. 濡木理

# of Collaborated Projects: 0 results

# of Collaborated Products: 6 results
11. 加藤英明

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
12. 木瀬孔明

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Shihoya Wataru 志甫谷 渉

Research Projects

Research Products

Co-Researchers

クライオ電子顕微鏡法を用いたGPCR標的型機能性抗体の開発と応用Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

GPCR drug discovery 2.0 targeting lysophospholipid-activated GPCRsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Structural analysis and drug development of GPCRsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Structural and funtinal analysis of non-cannonical rhodopsinsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

TR-SFX study of non-canonical rhodopsinPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Time-resolved XFEL crystallography and rational engineering of light-driven proteins

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Structural analysis of endothelin receptors in complex with G-proteinsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

[Journal Article] Cryo-EM advances in GPCR structure determination2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Molecular insights into atypical modes of β-arrestin interaction with seven transmembrane receptors2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Optimizing cryo-EM structural analysis of Gi-coupling receptors via engineered Gt and Nb35 application2024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Structural basis for lysophosphatidylserine recognition by GPR342024

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Phototrophy by Antenna-Containing Rhodopsin Pumps in Aquatic Environments2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Cryo-EM structure of the endothelin-1-ETB-Gi complex2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Structural Insights into Endothelin Receptor Signaling2023

Author(s)

Journal Title

DOI

Shihoya Wataru 志甫谷渉