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TAKAI Kazuyuki  高井 和幸

ORCIDConnect your ORCID iD *help
… Alternative Names

高井 和幸  タカイ カズユキ

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Researcher Number 40260848
Other IDs
External Links
Affiliation (Current) 2025: 愛媛大学, 理工学研究科(工学系), 教授
Affiliation (based on the past Project Information) *help 2016: 愛媛大学, 理工学研究科(工学系), 教授
2012 – 2015: 愛媛大学, 理工学研究科, 教授
2011: 愛媛大学, 大学院・理工学研究科, 教授
2008 – 2009: Ehime University, 無細胞生命科学工学研究センター, 准教授
2006: 愛媛大学, 理工学研究科, 助教授 … More
2004 – 2006: 愛媛大学, 無細胞生命科学工学研究センター, 助教授
1996 – 1999: 千葉工業大学, 工学部, 講師
1997: 東京医科歯科大学, 医学部, 講師
1994: 千葉工業大学, 工学部, 助手 Less
Review Section/Research Field
Principal Investigator
Living organism molecular science / System genome science / System genome science / Science and Engineering
Except Principal Investigator
極限環境生物学 / 広領域
Keywords
Principal Investigator
組換え発現 / コムギ / 再構成 / 翻訳因子 / the genetic code / RNA ligases / modified nucleoside / mRNA / イノシン / 遺伝暗号進化 … More / tRNA / 無細胞タンパク質合成系 / 修飾ウリジン / 分子生物学 / wobble則 / 遺伝暗号 / RNAリガーゼ / 修飾ヌクレオシド / 大腸菌由来無細胞タンパク質合成系 / CCT / SfiNX / コドン最適化 / 大腸菌 / シャペロニン / wheat / Escherichia coli / expression / chaperonin / 無細胞再構成 / 植物 / 合成生物学 / リボソーム / 翻訳 / タンパク質合成系 / タンパク質合成 / コムギ胚芽 … More
Except Principal Investigator
非イオン界面活性剤 / マグネシウムイオン / アンチセンスDNA / 耐分解酵素性 / 感染細胞 / レトロウイルス / RNase H活性 / センスRNA / RNAキメラオリゴマー / 環状DNA / エキソン / イントロン / スプライシング / Biomolecule / Evolution / Genome / Enzyme / Nucleic Acid / RNAメチル化酵素 / tRNA / 好熱菌 / RNA修飾 / 進化 / ゲノム / 生体分子 / 酵素 / 核酸 / nuclease resistance / HIV-1 infected cells / retrovirus / Rnase H / sense RNA / antisense DNA / DNA chimeric oligonucleotide / circular dumbbell RNA / 耐分解酵素 / RNaseH活性 / tRNA^<Tyr> / 5'-OH RNA / 2'、3'-環状リン酸 / ピリミジン-アデニン / RNAの位置特異的切断反応 / 逆転写酵素阻害能 / 構造タンパク質遺伝子 / 抗HIV活性 / 逆転写酵素 / ホスホリピドDNA / ホスホロチオエ-トオリゴマー Less
  • Research Projects

    (11 results)
  • Research Products

    (36 results)
  • Co-Researchers

    (13 People)
  •  Combining eukaryotic chaperonins with the translation system from Escherichia coliPrincipal Investigator

    • Principal Investigator
      Takai Kazuyuki
    • Project Period (FY)
      2014 – 2016
    • Research Category
      Grant-in-Aid for Challenging Exploratory Research
    • Research Field
      System genome science
    • Research Institution
      Ehime University
  •  Constitutive approach to the translation system from wheatPrincipal Investigator

    • Principal Investigator
      TAKAI Kazuyuki
    • Project Period (FY)
      2011 – 2014
    • Research Category
      Grant-in-Aid for Scientific Research (B)
    • Research Field
      System genome science
    • Research Institution
      Ehime University
  •  タンパク質合成系の再構成に向けたコムギ胚芽翻訳因子の分画Principal Investigator

    • Principal Investigator
      高井 和幸
    • Project Period (FY)
      2008 – 2009
    • Research Category
      Grant-in-Aid for Scientific Research on Priority Areas
    • Review Section
      Science and Engineering
    • Research Institution
      Ehime University
  •  Molecular Evolution of RNA modification enzymes and RNA recognition mechanism

    • Principal Investigator
      HORI Hiroyuki
    • Project Period (FY)
      2005 – 2006
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      極限環境生物学
    • Research Institution
      Ehime University
  •  Nucleoside modification and evolution of the wobble rulePrincipal Investigator

    • Principal Investigator
      TAKAI Kazuyuki
    • Project Period (FY)
      2004 – 2005
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Living organism molecular science
    • Research Institution
      Ehime University
  •  金属イオン-非イオン界面活性剤によるRNAの位置特異的切断反応

    • Principal Investigator
      高久 洋
    • Project Period (FY)
      1998
    • Research Category
      Grant-in-Aid for Scientific Research on Priority Areas (A)
    • Research Institution
      Chiba Institute of Technology
  •  ウイルス感染細胞内でアンチセンス機能を発揮するアンチセンス核酸の構築と感染制御

    • Principal Investigator
      高久 洋
    • Project Period (FY)
      1998
    • Research Category
      Grant-in-Aid for Scientific Research on Priority Areas (A)
    • Research Institution
      Chiba Institute of Technology
  •  金属イオン-非イオン界面活性剤によるRNAの位置特異的切断反応

    • Principal Investigator
      高久 洋
    • Project Period (FY)
      1997
    • Research Category
      Grant-in-Aid for Scientific Research on Priority Areas
    • Research Institution
      Chiba Institute of Technology
  •  Inhibition of HIV-1 replication of an HIV-1 dependent antisense oligonucleotides.

    • Principal Investigator
      TAKAKU Hiroshi
    • Project Period (FY)
      1997 – 1999
    • Research Category
      Grant-in-Aid for Scientific Research (A)
    • Research Field
      広領域
    • Research Institution
      Chiba Institute of Technology
  •  金属イオン-非イオン界面活性剤によるRNAの位置特異的切断反応

    • Principal Investigator
      高久 洋
    • Project Period (FY)
      1996
    • Research Category
      Grant-in-Aid for Scientific Research on Priority Areas
    • Research Institution
      Chiba Institute of Technology
  •  抗HIV活性をもつ機能性アンチセンスDNAの合成とその作用機序

    • Principal Investigator
      TAKAKU Hiroshi
    • Project Period (FY)
      1994
    • Research Category
      Grant-in-Aid for Scientific Research on Priority Areas
    • Research Institution
      Chiba Institute of Technology

All 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2005 Other

All Journal Article Presentation

  • [Journal Article] Translational resistivity/conductivity of coding sequences during exponential growth of Escherichia coli.2017

    • Author(s)
      Kazuyuki Takai
    • Journal Title

      Journal of Theoretical Biology

      Volume: 413 Pages: 66-71

    • DOI

      10.1016/j.jtbi.2016.11.015

    • Peer Reviewed / Acknowledgement Compliant / Open Access
    • Data Source
      KAKENHI-PROJECT-26640129
  • [Journal Article] CodHonEditor: Spreadsheets for Codon Optimization and Editing of Protein Coding Sequences2016

    • Author(s)
      Kazuyuki TAKAI
    • Journal Title

      Nucleosides, Nucleotides and Nucleic Acids

      Volume: 35 Issue: 5 Pages: 223-232

    • DOI

      10.1080/15257770.2015.1127962

    • Peer Reviewed / Acknowledgement Compliant / Open Access
    • Data Source
      KAKENHI-PROJECT-26640129
  • [Journal Article] SfiNX: a method for assembly of protein coding sequences with high success rates2016

    • Author(s)
      Kazuyuki TAKAI, Keigo HISAMATSU
    • Journal Title

      Biotechnology Letters

      Volume: 38 Issue: 5 Pages: 773-778

    • DOI

      10.1007/s10529-016-2042-2

    • Peer Reviewed / Acknowledgement Compliant / Open Access
    • Data Source
      KAKENHI-PROJECT-26640129
  • [Journal Article] Purification of Wheat Factors Involving in Translation Initiation for Reconstitution of Protein Synthesis.2009

    • Author(s)
      Nagano, H., Sugihara, S., Takagi, H., Ogasawara, T., Endo, Y., Takai, K.
    • Journal Title

      Proceedings of the 20th Annual Symposium on Micro-Nano Mechatronics and Human Sciences

      Pages: 214-219

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-20034040
  • [Journal Article] The wheat-germ cell-free expression system.2009

    • Author(s)
      Takai, K., Sawasaki, T., Endo, Y.
    • Journal Title

      Curr.Pharm.Biotech. 3

      Pages: 272-278

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-20034040
  • [Journal Article] Purification of Plant Translation Factors for Reconstituti on of Protein Synthesis2008

    • Author(s)
      H. Nagano, S. Sugihara, H. Takagi, T. Ogasawara, Y. Endo, and K. Takai
    • Journal Title

      Proceedings of the 19th Annual Symposium on Micro-Nano Mechatronics and Human Sciences

      Pages: 169-173

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-20034040
  • [Journal Article] Purification of eukaryotic translation factors from wheat germ for reconstitution of protein synthesis2008

    • Author(s)
      H. Nagano, S. Sugihara, H. Takagi, T. Ogasawara, Y. Endo, and K. Takai
    • Journal Title

      Nucleic Adds Symposium Ser 52

      Pages: 497-498

    • Data Source
      KAKENHI-PROJECT-20034040
  • [Journal Article] Purification and sequence determination of an RNA ligase from wheat embryos.2005

    • Author(s)
      Makino, S., Sawasaki, T., Endo, Y., Takai, K.
    • Journal Title

      Nucleic Acids Symposium Ser.49

      Pages: 319-320

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-16510162
  • [Journal Article] Possible conformation of 5-aminomethyluridine derivatives recognizing a G at the third position of the codon.2005

    • Author(s)
      Takai, K.
    • Journal Title

      Nucleic Acids Symposium Ser.49

      Pages: 317-318

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-16510162
  • [Journal Article] Possible conformation of 5-aminomethyluridine. derivatives recognizing a G at the third position of the codon.2005

    • Author(s)
      Kazuyuki Takai
    • Journal Title

      Nucleic Acids Symposium Ser. 49

      Pages: 317-318

    • Description
      「研究成果報告書概要(和文)」より
    • Data Source
      KAKENHI-PROJECT-16510162
  • [Journal Article] Possible conformations of 5-aminomethyluridine derivatives recognizing a G at the third position of the codon2005

    • Author(s)
      Kazuyuki Takai
    • Journal Title

      Nucleic Acids Symposium Series 42

      Pages: 317-318

    • Data Source
      KAKENHI-PROJECT-16510162
  • [Journal Article] Classification of the possible pairs between the first anticodon and the third codon positions based on a simple model assuming two geometries with which the pairing effectively potentiates the decoding complex

    • Author(s)
      Kazuyuki Takai
    • Journal Title

      Journal of Theoretical Biology (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Data Source
      KAKENHI-PROJECT-16510162
  • [Journal Article] Classification of the possible pairs between the first anticodon and the third codon positions based on a simple model assuming two geometries with which the pairing effectively potentiates the decoding complex

    • Author(s)
      Kazuyuki Takai
    • Journal Title

      J.Theor.Biol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-16510162
  • [Presentation] コムギ由来ペプチド鎖解離因子複合体の調製2016

    • Author(s)
      阿賀健,久松啓伍,冨川千恵,高井和幸
    • Organizer
      「細胞を創る」研究会9.0
    • Place of Presentation
      早稲田大学国際会議場,東京都・新宿区
    • Year and Date
      2016-11-21
    • Data Source
      KAKENHI-PROJECT-26640129
  • [Presentation] コムギ胚芽からのシャペロニンを含む画分の調製2016

    • Author(s)
      加藤凌平,冨川千恵,高井和幸
    • Organizer
      「細胞を創る」研究会9.0
    • Place of Presentation
      早稲田大学国際会議場,東京都・新宿区
    • Year and Date
      2016-11-21
    • Data Source
      KAKENHI-PROJECT-26640129
  • [Presentation] CodHonEditor:中身が見えるコドン最適化ツール2016

    • Author(s)
      高井和幸
    • Organizer
      「細胞を創る」研究会9.0
    • Place of Presentation
      早稲田大学国際会議場,東京都・新宿区
    • Year and Date
      2016-11-21
    • Data Source
      KAKENHI-PROJECT-26640129
  • [Presentation] タンパク質をコードするDNAの効率的な連結2015

    • Author(s)
      高井和幸
    • Organizer
      「細胞を創る」研究会8.0
    • Place of Presentation
      大阪大学吹田キャンパス,吹田市
    • Year and Date
      2015-11-12
    • Data Source
      KAKENHI-PROJECT-26640129
  • [Presentation] 転写後修飾によるウリジン3位のプロトン乖離2015

    • Author(s)
      高井和幸
    • Organizer
      BMB2015 第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド(神戸国際会議場),神戸
    • Year and Date
      2015-12-01
    • Invited
    • Data Source
      KAKENHI-PROJECT-26640129
  • [Presentation] Toward In Vitro Reconstitution of the Protein Synthesis System from Wheat.2014

    • Author(s)
      K. Takai
    • Organizer
      8th International Symposium on Nanomedicine - Fusion of Nanomedicine and Molecular Science of Photosynthesis -
    • Place of Presentation
      愛媛大学,愛媛県松山市
    • Year and Date
      2014-12-05
    • Invited
    • Data Source
      KAKENHI-PROJECT-23310141
  • [Presentation] コムギ由来翻訳開始因子eIF2の調製法の検討2013

    • Author(s)
      野中拓,久松啓伍,冨川千恵,高井和幸
    • Organizer
      第15回日本RNA学会年会
    • Place of Presentation
      松山
    • Data Source
      KAKENHI-PROJECT-23310141
  • [Presentation] コムギ由来翻訳開始因子eIF2の調製法の検討2013

    • Author(s)
      野中拓,久松啓伍,冨川千恵,高井和幸
    • Organizer
      「細胞を創る」研究会6.0
    • Place of Presentation
      鶴岡
    • Data Source
      KAKENHI-PROJECT-23310141
  • [Presentation] コムギ由来タンパク質合成関連因子の一次配列情報2012

    • Author(s)
      高井和幸,久松啓伍
    • Organizer
      「細胞を創る」研究会6.0
    • Place of Presentation
      東京工業大学すずかけ台キャンパス
    • Data Source
      KAKENHI-PROJECT-23310141
  • [Presentation] タンパク質合成の再構成に向けた真核生物翻訳因子の調製2011

    • Author(s)
      久松啓伍, 小笠原富夫, 遠藤弥重太, 高井和幸
    • Organizer
      第6回無細胞生命科学研究会
    • Place of Presentation
      兵庫県立大学書写記念会館(姫路)
    • Data Source
      KAKENHI-PROJECT-23310141
  • [Presentation] Preparation of eukaryotic translation factors for reconstitution of protein synthesis2011

    • Author(s)
      Keigo Hisamatsu, Tomio Ogasawara, Yaeta Endo, Kazuyuki Takai
    • Organizer
      International Symposium on SYNTHESIZING LIFE AND BIOLOGICAL SYSTEMS & The Fourth Annual Meeting of the Japanese Society for Cell Synthesis Research
    • Place of Presentation
      千里ライフサイエンスセンター(吹田)
    • Data Source
      KAKENHI-PROJECT-23310141
  • [Presentation] 真核型再構成タンパク質合成系を目指した取り組み2010

    • Author(s)
      長野光, 高木久徳, 小笠原富夫, 遠藤弥重太, 高井和幸
    • Organizer
      特定領域研究「バイオ操作」第8回公開シンポジウム
    • Place of Presentation
      福岡市
    • Year and Date
      2010-03-11
    • Data Source
      KAKENHI-PROJECT-20034040
  • [Presentation] 翻訳促進配列に結合するコムギ胚芽抽出液中の因子の解析2009

    • Author(s)
      深田聡, 小笠原富夫, 遠藤弥重太, 高井和幸
    • Organizer
      「細胞を創る」研究会2.0
    • Place of Presentation
      東京都文京区
    • Data Source
      KAKENHI-PROJECT-20034040
  • [Presentation] タンパク質合成の再構成を目指したコムギ翻訳因子の調製2009

    • Author(s)
      長野光, 杉原祥平, 高木久徳, 小笠原富夫, 遠藤弥重太, 高井和幸
    • Organizer
      第3回無細胞生命科学研究会
    • Place of Presentation
      弘前市
    • Data Source
      KAKENHI-PROJECT-20034040
  • [Presentation] A comparison of ribosome fractions from washed and unwashed wheat embryo preparations2009

    • Author(s)
      長野光, 杉原祥平, 高木久徳, 小笠原富夫, 遠藤弥重太, 高井和幸
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜市
    • Data Source
      KAKENHI-PROJECT-20034040
  • [Presentation] タンパク質合成の再構成を目指したコムギ翻訳因子の調製2009

    • Author(s)
      長野光, 杉原祥平, 高木久徳, 小笠原富夫, 遠藤弥重太, 高井和幸
    • Organizer
      特定領域研究「バイオ操作」第7回公開シンポジウム
    • Place of Presentation
      仙台市
    • Year and Date
      2009-03-06
    • Data Source
      KAKENHI-PROJECT-20034040
  • [Presentation] 洗浄済みコムギ胚芽および未洗浄コムギ胚芽から調製したリボソームの比較2009

    • Author(s)
      長野光, 杉原祥平, 高木久徳, 小笠原富夫, 遠藤弥重太, 高井和幸
    • Organizer
      「細胞を創る」研究会2.0
    • Place of Presentation
      東京都文京区
    • Data Source
      KAKENHI-PROJECT-20034040
  • [Presentation] コムギ無細胞系で内部からの翻訳開始を促進する新規配列の探索の試み2008

    • Author(s)
      笠井彰太郎, 高井和幸
    • Organizer
      「細胞を創る」研究会1.0
    • Place of Presentation
      吹田市
    • Data Source
      KAKENHI-PROJECT-20034040
  • [Presentation] コムギ胚芽無細胞タンパク質合成系-再構成に向けて-2008

    • Author(s)
      高井和幸
    • Organizer
      「細胞を創る」研究会1.0
    • Place of Presentation
      吹田市
    • Data Source
      KAKENHI-PROJECT-20034040
  • [Presentation] タンパク質合成の再構成に向けたコムギ翻訳因子の精製2008

    • Author(s)
      長野光, 杉原祥平, 高木久徳, 小笠原富夫, 遠藤弥重太, 高井和幸
    • Organizer
      「細胞を創る」研究会1.0
    • Place of Presentation
      吹田市
    • Data Source
      KAKENHI-PROJECT-20034040
  • [Presentation] Purification of wheat translation factors for reconstitution of oprotein synthesis2008

    • Author(s)
      長野光, 杉原祥平, 高木久徳, 小笠原富夫, 遠藤弥重太, 高井和幸
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸市
    • Data Source
      KAKENHI-PROJECT-20034040
  • [Presentation] 大腸菌を用いたコムギ由来翻訳開始因子eIF2Bの調製の試み

    • Author(s)
      上野秀道,高井和幸,冨川千恵
    • Organizer
      「細胞を創る」研究会7.0
    • Place of Presentation
      東京大学,東京都
    • Year and Date
      2014-11-13 – 2014-11-14
    • Data Source
      KAKENHI-PROJECT-23310141
  • [Presentation] SfiI認識部位を導入した大腸菌共発現ベクターの試み

    • Author(s)
      高井和幸
    • Organizer
      「細胞を創る」研究会7.0
    • Place of Presentation
      東京大学,東京都
    • Year and Date
      2014-11-13 – 2014-11-14
    • Data Source
      KAKENHI-PROJECT-23310141
  • 1.  TAKAKU Hiroshi (50101267)
    # of Collaborated Projects: 6 results
    # of Collaborated Products: 0 results
  • 2.  YAMAMOTO Naoki (00094053)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 3.  HORI Hiroyuki (20256960)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 4.  NUREKI Osamu (10272460)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 5.  中島 秀喜 (20192669)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 6.  HISAMATSU Keigo
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 5 results
  • 7.  TOMIKAWA Chie
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 3 results
  • 8.  KISHIMOTO Tatsuro
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 9.  KANNO Keisuke
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 10.  UENO Hidemichi
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 1 results
  • 11.  KONDO Masaki
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 12.  TOKUMARU Tatsuya
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 13.  NONAKA Taku
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 2 results

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