Kinoshita Emiko 木下恵美子

… Alternative Names

KINOSHITA Emiko 木下恵美子

Less

Researcher Number	40379912
Other IDs
External Links
Affiliation (Current)	2025: 広島都市学園大学, 健康科学部, 教授(移行)
Affiliation (based on the past Project Information) *help	2019 – 2024: 広島大学, 医系科学研究科(薬), 助教 2017 – 2018: 広島大学, 医歯薬保健学研究科(薬), 助教 2014 – 2016: 広島大学, 医歯薬保健学研究院(薬), 助教 2012 – 2014: 広島大学, 医歯薬保健学研究院, 助教 2008 – 2011: Hiroshima University, 大学院・医歯薬学総合研究科, 助教 2007: Hiroshima University, 大学院・医術薬学総合研究科, 助教 2006: 広島大学, 技術センター, 技術主任
Review Section/Research Field	Principal Investigator Physical pharmacy / Basic Section 47010:Pharmaceutical chemistry and drug development sciences-related / Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related / Medical pharmacy
Keywords	Principal Investigator Phos-tag / フォスタグ / リン酸化 / 分析化学 / 電気泳動 / 抗生物質 / リン酸化タンパク質 / phos-tag / Phos-tag SDS-PAGE / バイオテクノロジー … More / 蛋白質 / 分子認識 / ナノ材料 / ヒスチジンキナーゼ阻害剤 / 2成分伝達系 / 阻害剤 / レスポンスレギュレータ / ２成分伝達系 / ヒスチジンキナーゼ / 疾患関連タンパク質 / シグナル伝達 / キナーゼ / ATPγS / 反応中間体 / リン酸基転移反応 / ATPgS / チオリン酸基 / リン酸基 / ATPgammaS / チオリン酸化タンパク質 / リン酸化シグナル / タンパク質 / 亜鉛フォスタグ / リン酸基親和性電気泳動法 / リン酸基親和性電気泳動 / β-カテニン / 蛍光ディファレンシャル電気泳動法 / 卵白アルブミン / βカテニン / ユビキチン化 / 翻訳後修飾 / リン酸親和性2次元電気泳動法 / オーダーメード医療 / オーダーメー医療 / シトシン / グアニン / サイクレン / 亜鉛 / SNP / 一塩基多型 Less

病原菌のリン酸化酵素阻害剤（抗生物質）の多検体高速スクリーニングシステムの開発Principal Investigator
- Principal Investigator
  
  木下恵美子
- Project Period (FY)
  2024 – 2026
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 47010:Pharmaceutical chemistry and drug development sciences-related
- Research Institution
  Hiroshima University
Generating proteomic data on signalling pathways in pathogenic bacteria for the discovery of new antibiotic target molecules.Principal Investigator
- Principal Investigator
  
  Kinoshita Emiko
- Project Period (FY)
  2021 – 2023
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 47010:Pharmaceutical chemistry and drug development sciences-related
- Research Institution
  Hiroshima University
Development of a system for profiling the pathophysiology of diseases from the phosphorylated states of proteinsPrincipal Investigator
- Principal Investigator
  
  Kinoshita Emiko
- Project Period (FY)
  2018 – 2020
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
- Research Institution
  Hiroshima University
Creation of the analysis technique to visualize the dynamics of labile intermediates in phosphotransfer reactionsPrincipal Investigator
- Principal Investigator
  
  Kinoshita Emiko
- Project Period (FY)
  2015 – 2017
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Physical pharmacy
- Research Institution
  Hiroshima University
Development of phosphate affinity electrophoresis system for phosphorylation profiling of proteins with a wide range of molecular masses.Principal Investigator
- Principal Investigator
  
  KINOSHITA Emiko
- Project Period (FY)
  2012 – 2014
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Physical pharmacy
- Research Institution
  Hiroshima University
A method for simultaneous detection of dual post-translational modifications using phosphate-affinity electrophoresisPrincipal Investigator
- Principal Investigator
  
  KINOSHITA Emiko
- Project Period (FY)
  2010 – 2011
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Physical pharmacy
- Research Institution
  Hiroshima University
Development of phosphate-affinity two-dimensional fluorescence difference gel electrophoresisPrincipal Investigator
- Principal Investigator
  
  KINOSHITA Emiko
- Project Period (FY)
  2008 – 2009
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Physical pharmacy
- Research Institution
  Hiroshima University
オーダーメード医療を目的とした迅速かつ網羅的な疾患遺伝子多型検出法の開発Principal Investigator
- Principal Investigator
  
  木下恵美子
- Project Period (FY)
  2006 – 2007
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Medical pharmacy
- Research Institution
  Hiroshima University

[Book] Neuroproteomics: Neuromthods, 146 (ed. by Li, KW)2019
- Author(s)
  Eiji Kinoshita, Kinoshita-Kikuta, E., Koike, T.
- Total Pages
  218
- Publisher
  Springer Nature
- ISBN
  9781617791116
- Data Source
  KAKENHI-PROJECT-18K06596
[Book] Phosphopeptide detection with biotin- labeled Phos- tag, in Phospho- proteomics: Methods and Protocols, Methods in Molecular Biology, 13552016
- Author(s)
  Kinoshita- Kikuta, E., Eiji Kinoshita, and Koike, T.
- Total Pages
  13
- Publisher
  Springer
- Data Source
  KAKENHI-PROJECT-15K07887
[Book] Phis-Tag based affinity chromatography techniques for enrichment of the phosphoproteome, in Protein Modifications in Pathogenic Dysregulation of Signaling.2015
- Author(s)
  Eiji Kinoshita,Kinoshita- Kikuta, E.,and Koike, T.
- Total Pages
  14
- Publisher
  Springer
- Data Source
  KAKENHI-PROJECT-15K07887
[Book] Phos- tag technology for kinomics, in Kinomics: Approaches and Applications.2015
- Author(s)
  Kinoshita- Kikuta, E., Eiji Kinoshita, and Koike, T.
- Total Pages
  16
- Publisher
  Wiley- VCH
- Data Source
  KAKENHI-PROJECT-15K07887
[Book] Neutral phosphate affinity SDS- PAGE system for profiling of protein phosphorylation, in Proteomic Profiling: Methods and Protocols,Methods in Molecular Biology, 12952015
- Author(s)
  Kinoshita- Kikuta, E., Eiji Kinoshita, and Koike, T.
- Total Pages
  32
- Publisher
  Springer
- Data Source
  KAKENHI-PROJECT-15K07887
[Book] DNA Replication and Mutation2012
- Author(s)
  Kinoshita, E. Kinoshita-Kikuta, E. Koike, T.
- Total Pages
  21
- Publisher
  Nova Science Publishers
- Data Source
  KAKENHI-PROJECT-24590050
[Book] Phos-tag affinity electrophoresis for protein kinase profiling.2012
- Author(s)
  Kinoshita, E. Kinoshita-Kikuta, E. Koike, T.
- Total Pages
  22
- Publisher
  Springer
- Data Source
  KAKENHI-PROJECT-24590050
[Book] 臨床プロテオミクス～バイオマーカー探索から個別化医療へ2012
- Author(s)
  木下英司, 木下恵美子，小池透
- Total Pages
  5
- Publisher
  金原出版
- Data Source
  KAKENHI-PROJECT-24590050
[Book] Phosphate-affinity polyacrylamide gel electrophoresis for SNP genotyping. in Single Nucleotide Polymorphisms2009
- Author(s)
  Kinoshita, E.
- Total Pages
  10
- Publisher
  Humana Press
- Data Source
  KAKENHI-PROJECT-20790036
[Book] Zn(II)-cyclen polyacrylamide gel electrophoresis for SNP detection. in Single Nucleotide Polymorphisms, Methods in Molecular Biology, 578 (ed.by Komar AA)2009
- Author(s)
  Kinoshita-Kikuta, E.
- Total Pages
  14
- Publisher
  Humana Press
- Data Source
  KAKENHI-PROJECT-20790036
[Book] 細胞内リン酸化タンパク質を高精度に検出するためのPhos-tagビーズによるサンプル前処理法.細胞工学2009
- Author(s)
  木下英司
- Total Pages
  5
- Publisher
  秀潤社
- Data Source
  KAKENHI-PROJECT-20790036
[Book] 細胞内リン酸化タンパク質を高精度に検出するためのPhos-tagビーズによるサンプル前処理法2009
- Author(s)
  木下英司, 木下恵美子, 小池透
- Publisher
  秀潤社
- Data Source
  KAKENHI-PROJECT-20790036
[Book] Methods in Molecular Biology, 578 (ed. by Komar AA)2009
- Author(s)
  Emiko Kinoshita-Kikuta, Kinoshita, E., Koike, T
- Publisher
  Humana Press
- Data Source
  KAKENHI-PROJECT-20790036
[Book] Methods in Molecular Biology, 578 (ed. by Komar AA)2009
- Author(s)
  K inoshita, E., Emiko Kinoshita-Kikuta, Koike, T
- Publisher
  Humana Press
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] Characterization of Zinc(II) Complex of 1,4,7,10-Tetrazacyclododecane and Deprotonated 5-Fluorouracil (FU) in Crystalline/Solution States and Evaluation of Anticancer Activity: Approach for Improving the Anticancer Activity of FU2023
- Author(s)
  Yoshimi Ichimaru, Koichi Kato, Rina Nakatani, Kirara Sugiura, Hideki Mizutani, Emiko Kinoshita-Kikuta, Tohru Koike, Wanchun Jin, Masanori Imai, Hiromasa Kurosaki
- Journal Title
  
  Inorganic Chemistry Communications
  
  Volume: 147 Pages: 110221-110221
- DOI
  10.1016/j.inoche.2022.110221
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K07210, KAKENHI-PROJECT-21K06455, KAKENHI-PROJECT-21K15244, KAKENHI-PROJECT-20K06988
[Journal Article] Characterization of phosphorylation status and kinase activity of Src family kinases expressed in cell-based and cell-free protein expression systems2022
- Author(s)
  木下恵美子，木下英司，小池透
- Journal Title
  
  Electrophoresis Letters
  
  Volume: 66 Issue: 2 Pages: 71-79
- DOI
  10.2198/electroph.66.71
- ISSN
  2189-2628, 2189-2636
- Language
  Japanese
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-21K06455
[Journal Article] Use of Escherichia coli expression system for analyzing kinase motifs2022
- Author(s)
 Kinoshita-Kikuta, E., Ino, Y., Kimura, Y., Akiyama, T., Kinoshita, E., Koike, T.
- Journal Title
 
 J. Electrophor
 
 Volume: 66 Issue: 1 Pages: 13-20
- DOI
 10.2198/jelectroph.66.13
- ISSN
 1349-9394, 1349-9408
- Language
 Japanese
- Peer Reviewed / Open Access
- Data Source
 KAKENHI-PROJECT-21K06455, KAKENHI-PROJECT-20K06988
[Journal Article] Crystal Structure of Bis{1,3-bis[bis(pyridin-2-ylmethyl)amino]propan-2-olato-dizinc(II)}orthophosphate Tris(perchlorate) Octahydrate, [(Phos-tag)2-PO43−][ClO4−]3·8H2O2021
- Author(s)
 Ichimaru Yoshimi、Kato Koichi、Jin Wanchun、Sugiura Kirara、Kinoshita-Kikuta Emiko、KINOSHITA Eiji、Kurosaki Hiromasa、Koike Tohru
- Journal Title
 
 X-ray Structure Analysis Online
 
 Volume: 37 Issue: 0 Pages: 87-88
- DOI
 10.2116/xraystruct.37.87
- NAID
 130008127785
- ISSN
 1883-3578
- Year and Date
 2021-12-10
- Language
 English
- Peer Reviewed / Open Access
- Data Source
 KAKENHI-PROJECT-17K08376, KAKENHI-PROJECT-20K06988, KAKENHI-PROJECT-21K06455, KAKENHI-PROJECT-21K06500
[Journal Article] Recent advances in the Phos-tag technique focused on the analysis of phosphoproteins in a bacterial two-component system.2021
- Author(s)
  Kinoshita-Kikuta E., Koike T., Kinoshita E
- Journal Title
  
  J. Proteomics
  
  Volume: 252 Pages: 10429-10429
- DOI
  10.1016/j.jprot.2021.104429
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-21K06455
[Journal Article] Evaluation of four phosphopeptide enrichment strategies for mass spectrometry‐based proteomic analysis2021
- Author(s)
  Ino Yoko、Kinoshita Eiji、Kinoshita‐Kikuta Emiko、Akiyama Tomoko、Nakai Yusuke、Nishino Kohei、Osada Makoto、Ryo Akihide、Hirano Hisashi、Koike Tohru、Kimura Yayoi
- Journal Title
  
  PROTEOMICS
  
  Volume: 22 Issue: 7 Pages: 2100216-2100216
- DOI
  10.1002/pmic.202100216
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19H03774, KAKENHI-PROJECT-20H03498, KAKENHI-PROJECT-20K06988, KAKENHI-PROJECT-21K06455
[Journal Article] An assay of human tyrosine protein kinase ABL activity using an Escherichia coli protein expression system.2021
- Author(s)
  Kinoshita-Kikuta E, Yoshimoto M, Yano M, Eiji Kinoshita, Koike T.
- Journal Title
  
  Biotechniques
  
  Volume: 70(4) Issue: 4 Pages: 209-217
- DOI
  10.2144/btn-2020-0154
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-20K06988, KAKENHI-PROJECT-21K06455
[Journal Article] History of Phos-tag technology for phosphoproteomics.2021
- Author(s)
  Kinoshita E., Kinoshita-Kikuta E., Koike T
- Journal Title
  
  J. Proteomics
  
  Volume: 252 Pages: 104432-104432
- DOI
  10.1016/j.jprot.2021.104432
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-21K06455
[Journal Article] Characterization of phosphorylation status and kinase activity of Src family kinases expressed in cell-based and cell-free protein expression system.2021
- Author(s)
  Kinoshita-Kikuta E, Eiji Kinoshita, Suga M, Higashida M, Yamane Y, Nakamura T, Koike T.
- Journal Title
  
  Biomolecules
  
  Volume: 11(10) Issue: 10 Pages: 1448-1448
- DOI
  10.3390/biom11101448
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-20K06988, KAKENHI-PROJECT-21K06455
[Journal Article] Quantitative analysis of phosphoproteins in a bacterial two-component system using Phos-tag techniques2020
- Author(s)
  Kinoshita-Kikuta Emiko、Kinoshita Eiji、Koike Tohru
- Journal Title
  
  Electrophoresis Letters
  
  Volume: 64 Issue: 1 Pages: 35-39
- DOI
  10.2198/electroph.64.35
- NAID
  130007871304
- ISSN
  2189-2628, 2189-2636
- Language
  Japanese
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-18K06596
[Journal Article] A dot-blot-staining method for detecting phosphoproteins with a Phos-tag Aqua fluorescent dye2020
- Author(s)
  Kinoshita-Kikuta Emiko、Akayama Keisuke、Kinoshita Eiji、Koike Tohru
- Journal Title
  
  J. Electrophor
  
  Volume: 64 Issue: 1 Pages: 7-11
- DOI
  10.2198/jelectroph.64.7
- NAID
  130007961315
- ISSN
  1349-9394, 1349-9408
- Language
  English
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-20K06988, KAKENHI-PROJECT-18K06596
[Journal Article] Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during intracellular protein traffic2020
- Author(s)
  Kinoshita-Kikuta Emiko、Utsumi Toshihiko、Miyazaki Aya、Tokumoto Chiharu、Doi Kyosuke、Harada Haruna、Kinoshita Eiji、Koike Tohru
- Journal Title
  
  Scientific Reports
  
  Volume: 10 Issue: 1 Pages: 16273-16273
- DOI
  10.1038/s41598-020-73248-0
- NAID
  120007169771
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-20K05827, KAKENHI-PROJECT-20K06988, KAKENHI-PROJECT-18K06596
[Journal Article] An immuno-dot blot assay for screening histidine kinase inhibitors2020
- Author(s)
  Kinoshita-Kikuta Emiko、Maruta Shino、Eguchi Yoko、Igarashi Masayuki、Okajima Toshihide、Utsumi Ryutaro、Kinoshita Eiji、Koike Tohru
- Journal Title
  
  Analytical Biochemistry
  
  Volume: 600 Pages: 113765-113765
- DOI
  10.1016/j.ab.2020.113765
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-20K06988, KAKENHI-PROJECT-18K06596
[Journal Article] A strategy to identify protein-N-myristoylation-dependent phosphorylation reactions of cellular proteins by using Phos-tag SDS-PAGE.2019
- Author(s)
  Emiko Kinoshita-Kikuta, Ayane Tanikawa, Takuro Hosokawa, Aya Kiwado, Koko Moriya, Eiji Kinoshita, Tohru Koike, Toshihiko Utsumi
- Journal Title
  
  PLOS ONE
  
  Volume: 14 Issue: 11 Pages: e0225510-e0225510
- DOI
  10.1371/journal.pone.0225510
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K06596, KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-17K07758, KAKENHI-PROJECT-17K08237
[Journal Article] Increase in constitutively active MEK1 species by introduction of MEK1 mutations identified in cancers2019
- Author(s)
  E. Kinoshita-Kikuta, E. Kinoshita, S. Ueda, Y. Ino, Y. Kimura, H. Hirano, and T. Koike
- Journal Title
  
  BBA - Proteins and Proteomics
  
  Volume: 1867 Issue: 1 Pages: 62-70
- DOI
  10.1016/j.bbapap.2018.05.004
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-17K08237, KAKENHI-PROJECT-18K06596, KAKENHI-PROJECT-16H05230
[Journal Article] Quantitative monitoring of His and Asp phosphorylation in a bacterial signaling system by using Phos-tag Magenta/Cyan fluorescent dyes2019
- Author(s)
  Emiko Kinoshita-Kikuta, Hiroshi Kusamoto, Syogo Ono, Keisuke Akayama, Yoko Eguchi, Masayuki Igarashi, Toshihide Okajima, Ryutaro Utsumi, Eiji Kinoshita, Tohru Koike
- Journal Title
  
  Electrophoresis
  
  Volume: 40 Issue: 22 Pages: 3005-3013
- DOI
  10.1002/elps.201900261
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K06596, KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-17K08237
[Journal Article] Gel-based analysis of protein phosphorylation status by rapid fluorometric staining using TAMRA-labeled Phos-tag2019
- Author(s)
  Hiroshi Kusamoto, Emiko Kinoshita-Kikuta, Tomoyo Nishimura, Tomomi Nagai, Eiji Kinoshita, and Tohru Koike,
- Journal Title
  
  J. Electrophor
  
  Volume: 63 Issue: 1 Pages: 25-32
- DOI
  10.2198/jelectroph.63.25
- NAID
  130007634033
- ISSN
  1349-9394, 1349-9408
- Language
  English
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K06596, KAKENHI-PROJECT-19K07147, KAKENHI-PROJECT-17K08237
[Journal Article] A method for profiling the phosphorylation state of tyrosine protein kinases.2018
- Author(s)
  Yuuki Uezato, Isamu Kameshita, Keiko Morisawa, Shuji Sakamoto, Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tohru Koike, Yasunori Sugiyama,
- Journal Title
  
  Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
  
  Volume: 1867 Issue: 1 Pages: 71-75
- DOI
  10.1016/j.bbapap.2018.05.003
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K06596, KAKENHI-PROJECT-16K08590
[Journal Article] Specific glutamic acid residues in targeted proteins induce exaggerated retardations in Phos-tag SDS-PAGE migration2017
- Author(s)
  E.Kinoshita, E. Kinoshita-Kikuta, K. Karata, T. Kawano, A. Nishiyama, M. Yamato, and T. Koike
- Journal Title
  
  Electrophoresis
  
  Volume: 38 Issue: 8 Pages: 1139-1146
- DOI
  10.1002/elps.201600520
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-26460036, KAKENHI-PROJECT-15K00541, KAKENHI-PROJECT-15K07887
[Journal Article] TAMRA/TAMRA fluorescence quenching systems for the activity assay of alkaline phosphatase2017
- Author(s)
  A. Shiba, E. Kinoshita-Kikuta, E. Kinoshita, and Tohru Koike
- Journal Title
  
  Sensors
  
  Volume: 17 Issue: 8 Pages: 1877-1888
- DOI
  10.3390/s17081877
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-17K08237, KAKENHI-PROJECT-15K07887
[Journal Article] Zn(II)-Phos-tag SDS-PAGE for Separation and Detection of a DNA Damage-Related Signaling Large Phosphoprotein2017
- Author(s)
  Kinoshita E., Kinoshita-Kikuta E., Koike T.
- Journal Title
  
  Methods and Protocols, Methods in Molecular Biology
  
  Volume: 1599 Pages: 113-126
- DOI
  10.1007/978-1-4939-6955-5_9
- ISBN
  9781493969531, 9781493969555
- Data Source
  KAKENHI-PROJECT-15K07887
[Journal Article] A Phos-tag SDS-PAGE method that effectively uses phosphoproteomic data for profiling the phosphorylation dynamics of MEK1.2016
- Author(s)
  Kinoshita E, Kinoshita-Kikuta E, Kubota Y, Takekawa M and Koike T.
- Journal Title
  
  Proteomics
  
  Volume: 16 Issue: 13 Pages: 1825-1836
- DOI
  10.1002/pmic.201500494
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PUBLICLY-15H01205, KAKENHI-ORGANIZER-16H06573, KAKENHI-PROJECT-15H04703, KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-26460036, KAKENHI-PROJECT-15K07887, KAKENHI-PROJECT-16K15212, KAKENHI-INTERNATIONAL-16K21739, KAKENHI-PLANNED-16H06574, KAKENHI-PROJECT-15K19019
[Journal Article] Validation of cis and trans modes in multistep phosphotransfer signaling of bacterial tripartite sensor kinases by using Phos- tag SDS- PAGE.2016
- Author(s)
  Kinoshita- Kikuta, E., Eiji Kinoshita, Eguchi, Y., Koike, T.
- Journal Title
  
  PLoS One
  
  Volume: 11 Issue: 2 Pages: e0148294-e0148294
- DOI
  10.1371/journal.pone.0148294
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-15K07887, KAKENHI-PROJECT-25560417, KAKENHI-PROJECT-25293005
[Journal Article] A novel thiol-affinity micropipette tip method using zinc(II)-cyclen-attached agarose beads for enrichment of cysteine-containing molecules.2016
- Author(s)
  Kusamoto, H., Shiba, A., Koretake, N., Fujioka, H., Hieda, Y., Kinoshita-Kikuta, E., Kinoshita, E., Koike, T.
- Journal Title
  
  J. Chromatogr. B. Analyt. Technol. Biomed. Life. Sci.
  
  Volume: 1031 Pages: 195-201
- DOI
  10.1016/j.jchromb.2016.07.049
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-15K07887
[Journal Article] Phosphopeptide detection with biotin-labeled Phos-tag.2016
- Author(s)
  Kinoshita-Kikuta, E., Kinoshita, E., and Koike, T.
- Journal Title
  
  Methods and Protocols, Methods in Molecular Biology
  
  Volume: 1355 Pages: 17-29
- DOI
  10.1007/978-1-4939-3049-4_2
- ISBN
  9781493930487, 9781493930494
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-15K07887
[Journal Article] Phos-tag SDS-PAGEゲルからの標的リン酸化タンパク質の転写効率改善2015
- Author(s)
  木下英司，木下恵美子，小池透
- Journal Title
  
  分析化学
  
  Volume: 64 Pages: 501-509
- NAID
  130005091793
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-15K07887
[Journal Article] Expression and phosphorylation state analysis of intracellular protein kinases using Multi- PK antibody and Phos- tag SDS- PAGE.2015
- Author(s)
  Sugiyama, Y., Katayama, S., Kameshita, I., Morisawa, K., Higuchi, T., Todaka, H., Eiji Kinoshita, Kinoshita- Kikuta, E., Koike, T., Taniguchi, T., Sakamoto, S.
- Journal Title
  
  MethodsX
  
  Volume: 2 Pages: 469-474
- DOI
  10.1016/j.mex.2015.11.007
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K07887, KAKENHI-PROJECT-26460036, KAKENHI-PROJECT-26750372, KAKENHI-PROJECT-14J07002, KAKENHI-PROJECT-25460371, KAKENHI-PROJECT-25560417, KAKENHI-PROJECT-25293005
[Journal Article] Advances in Phos- tag- based methodologies for separation and detection of the phosphoproteome.2015
- Author(s)
  Eiji Kinoshita, Kinoshita- Kikuta, E., and Koike, T.
- Journal Title
  
  Biochim. Biophys. Acta
  
  Volume: 1854 Issue: 6 Pages: 601-618
- DOI
  10.1016/j.bbapap.2014.10.004
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-15K07887, KAKENHI-PROJECT-25560417, KAKENHI-PROJECT-25293005
[Journal Article] The cutting edge of affinity electrophoresis technology2015
- Author(s)
  Eiji Kinoshita, Kinoshita-Kikuta, E., and Koike, T.
- Journal Title
  
  Proteomes
  
  Volume: 3 Issue: 1 Pages: 42-55
- DOI
  10.3390/proteomes3010042
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-25117718, KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-25560417, KAKENHI-PROJECT-15K07887
[Journal Article] Neutral phosphate affinity SDS-PAGE system for profiling of protein phosphorylation2015
- Author(s)
  Kinoshita-Kikuta, E., Eiji Kinoshita, and Koike, T.
- Journal Title
  
  Methods Mol. Biol.
  
  Volume: 1295 Pages: 323-354
- DOI
  10.1007/978-1-4939-2550-6_24
- ISBN
  9781493925490, 9781493925506
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-25117718, KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-25560417
[Journal Article] Simple enrichment of thiol- containing biomolecules by using zinc(II)-cyclen-functionalized magnetic beads2014
- Author(s)
  Fujioka, H., Tsunehiro, M., Kawaguchi, M., Kuramoto, Y., Kurosaki, H., Hieda, Y., Kinoshita-Kikuta, E., Eiji Kinoshita, and Koike, T.
- Journal Title
  
  J. Sep. Sci.
  
  Volume: 37 Issue: 13 Pages: 1601-1609
- DOI
  10.1002/jssc.201400135
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-25117718, KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-25560417
[Journal Article] Identification of two phosphorylated species of β-catenin involved in the ubiquitin-proteasome pathway by using two- dimensional Phos-tag affinity electrophoresis2014
- Author(s)
  Kinoshita-Kikuta, E., Eiji Kinoshita, and Koike, T.
- Journal Title
  
  J. Electrophoresis
  
  Volume: 58 Pages: 1-4
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-24590050
[Journal Article] Advances in Phos-tag-based methodologies for separation and detection of the phosphoproteome2014
- Author(s)
  Eiji Kinoshita, Kinoshita-Kikuta, E., and Koike, T.
- Journal Title
  
  Biochim. Biophys. Acta.
  
  Volume: 1854 Pages: 601-608
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-24590050
[Journal Article] アフィニティー電気泳動技術の最前線2014
- Author(s)
  木下英司,木下恵美子,小池透
- Journal Title
  
  ぶんせき
  
  Volume: 7 Pages: 365-371
- NAID
  40020143674
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-24590050
[Journal Article] Tips on improving the efficiency of electrotransfer of target proteins from Phos-tag SDS-PAGE gel2014
- Author(s)
  Kinoshita-Kikuta, E., Eiji Kinoshita, Matsuda, A., and Koike, T.
- Journal Title
  
  Proteomics
  
  Volume: 14 Issue: 21-22 Pages: 2437-2442
- DOI
  10.1002/pmic.201400380
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-25117718, KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-25560417
[Journal Article] Profiling of Protein Thiophosphorylation Using Phos-tag Affinity Electrophoresis: Evaluation of Adenosine 5′-O-(3-thiotriphosphate) as a Phosphoryl Donor in Protein Kinase Reactions2014
- Author(s)
  E. Kinoshita, E. Kinoshita-Kikuta, A. Shiba, K. Edahiro, Y. Inoue, K. Yamamoto, and T. Koike
- Journal Title
  
  Proteomics
  
  Volume: 14 Issue: 6 Pages: 668-679
- DOI
  10.1002/pmic.201300533
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-25117718, KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-25560417
[Journal Article] A Phos-tag-based fluorescence quenching system for activity assay and inhibitor screening for alkaline phosphatase2014
- Author(s)
  Kinoshita-Kikuta, E., Kurosaki, H., Kunisada, N., Eiji Kinoshita, and Koike, T.
- Journal Title
  
  Am. J. Anal. Chem.
  
  Volume: 5 Issue: 12 Pages: 796-804
- DOI
  10.4236/ajac.2014.512088
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-25117718, KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-25560417, KAKENHI-PROJECT-26460036
[Journal Article] A Phos-tag-based magnetic-bead method for rapid and selective separation of phosphorylated biomolecules2013
- Author(s)
  M. Tsunehiro, Y. Meki, K. Matsuoka, E. Kinoshita-Kikuta, E. Kinoshita and T. Koike
- Journal Title
  
  Journal of Chromatography B
  
  Volume: 925 Pages: 86-94
- DOI
  10.1016/j.jchromb.2013.02.039
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-22590037, KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-25117718, KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-25560417, KAKENHI-PUBLICLY-23117522
[Journal Article] Phos-tag-Based Microarray Techniques Advance Phosphoproteomics2013
- Author(s)
  Eiji Kinoshita, Emiko Kinoshita-Kikuta and Tohru Koike
- Journal Title
  
  Journal of Proteomics & Bioinformatics
  
  Volume: S6 Issue: S6 Pages: 8-8
- DOI
  10.4172/jpb.s6-008
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-25117718, KAKENHI-PROJECT-25293005
[Journal Article] Sandwich assay for phosphorylation of protein multiplexes by using antibodies and Phos-tag2013
- Author(s)
  E. Kinoshita, E. Kinoshita-Kikuta, and T. Koike
- Journal Title
  
  Analytical Biochemistry
  
  Volume: 438 Issue: 2 Pages: 104-106
- DOI
  10.1016/j.ab.2013.03.029
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-22590037, KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-25117718, KAKENHI-PROJECT-25293005, KAKENHI-PROJECT-25560417, KAKENHI-PUBLICLY-23117522
[Journal Article] Increase in Lipophilicity of Enalaprilat by Complexation with Copper(II) or Zinc(II) Ions.2013
- Author(s)
  Fujioka, H., Hieda, Y., Kuramoto, Y, Konishi, K., Kinoshita-Kikuta, E., Eiji Kinoshita, and Koike T.
- Journal Title
  
  Yakugaku Zasshi
  
  Volume: 33 Pages: 1135-1141
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24590050
[Journal Article] タンパク質リン酸化修飾の高感度検出法と新しいオミクス技術としての展開．2013
- Author(s)
  木下英司，木下恵美子，小池透
- Journal Title
  
  生化学
  
  Volume: 85 Pages: 447-552
- NAID
  10031184265
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-24590050
[Journal Article] Phos-tag SDS-PAGE systems for phosphorylation profiling of proteins with a wide range of molecular masses under neutral pH conditions2012
- Author(s)
  E. Kinoshita, E. Kinoshita-Kikuta, and T. Koike
- Journal Title
  
  Proteomics
  
  Volume: 12 Issue: 2 Pages: 192-202
- DOI
  10.1002/pmic.201100524
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-22590037, KAKENHI-PROJECT-22790037, KAKENHI-PUBLICLY-23117522
[Journal Article] Separation and identification of four distinct serine-phosphorylation states of ovalbumin by Phos-tag affinity electrophoresis2012
- Author(s)
  E.Kinoshita-Kikuta, E.Kinoshita, T.Koike
- Journal Title
  
  Electrophoresis
  
  Volume: (掲載決定)
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] Highly sensitive detectionof protein phosphorylation by usingimproved Phos-tag Biotin2012
- Author(s)
  Kinoshita E., Kinoshita-Kikuta E., Sugiyama Y., Fukuda Y., Ozeki T., andKoike T
- Journal Title
  
  in press
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] Separation andidentification of four distinctserine-phosphorylation states of ovalbuminby Phos-tag affinity electrophoresis2012
- Author(s)
  Kinoshita-Kikuta E., Kinoshita E., andKoike T
- Journal Title
  
  Electrophoresis
  
  Volume: in press
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] Phos-tag SDS-PAGEsystems for phosphorylation profiling ofproteins with a wide range of molecularmasses under neutral pH conditions2012
- Author(s)
  Kinoshita E., Kinoshita-Kikuta E., andKoike T
- Journal Title
  
  Proteomics
  
  Volume: 12 Pages: 192-202
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] 小池透プロトコール集フォスタグテクノロジーを用いたリン酸化タンパク質解析法2012
- Author(s)
  木下恵美子, 木下英司
- Journal Title
  
  生物物理化学
  
  Volume: 56 Pages: 51-75
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] 小池透フォスタグケミストリー2012
- Author(s)
  木下英司, 木下恵美子
- Journal Title
  
  生物物理化学
  
  Volume: 56 Pages: 3-7
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] 小池透今後の展望1中性条件で泳動を行う改良型Phos-tag SDS-PAGEを用いたリン酸化タンパク質解析2012
- Author(s)
  木下恵美子, 木下英司
- Journal Title
  
  生物物理化学
  
  Volume: 56 Pages: 41-44
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] 今後の展望1中性条件で泳動を行う改良型Phos-tag SDS-PAGEを用いたリン酸化タンパク質解析2012
- Author(s)
  木下恵美子, 木下英司, 小池透
- Journal Title
  
  生物物理化学
  
  Volume: 56
- NAID
  130004809455
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] Highly sensitive detection of protein phosphorylation by using improved Phos-tag Biotin2012
- Author(s)
  E.Kinoshita, E.Kinoshita-Kikuta, Y.Sugiyama, Y.Fukuda, T.Ozeki, T.Koike
- Journal Title
  
  Proteomics
  
  Volume: (掲載決定)
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] フォスタグケミストリー2012
- Author(s)
  木下英司, 木下恵美子, 小池透
- Journal Title
  
  生物物理化学
  
  Volume: 56
- NAID
  130004809452
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] 小池透今後の展望2蛍光性Phos-tagを用いたタンパク質リン酸化反応の解析法2012
- Author(s)
  木下英司, 木下恵美子, 多幾山敬, 宗村雅男
- Journal Title
  
  生物物理化学
  
  Volume: 56 Pages: 45-49
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] Highly sensitive detection of protein phosphorylation by using improved Phos-tag Biotin2012
- Author(s)
  E. Kinoshita, E. Kinoshita-Kikuta, Y. Sugiyama, Y. Fukada, T. Ozeki, and T. Koike
- Journal Title
  
  Proteomics
  
  Volume: 12 Issue: 7 Pages: 932-937
- DOI
  10.1002/pmic.201100639
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-22590037, KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-23117522
[Journal Article] プロトコール集フォスタグテクノロジーを用いたリン酸化タンパク質解析法2012
- Author(s)
  木下恵美子, 木下英司, 小池透
- Journal Title
  
  生物物理化学
  
  Volume: 56
- NAID
  130004809457
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] 今後の展望2蛍光性Phos-tagを用いたタンパク質リン酸化反応の解析法2012
- Author(s)
  木下英司, 木下恵美子, 多幾山敬, 宗村雅男, 小池透
- Journal Title
  
  生物物理化学
  
  Volume: 56
- NAID
  130004809456
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] A Laborsaving, Timesaving, and More Reliable Strategy for Separation of Low-Molecular-Mass Phosphoproteins in Phos-tag Affinity Electrophoresis2012
- Author(s)
  E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike
- Journal Title
  
  International Journal of Chemistry
  
  Volume: 4 Issue: 5 Pages: 1-8
- DOI
  10.5539/ijc.v4n5p1
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-22590037, KAKENHI-PROJECT-24590050, KAKENHI-PUBLICLY-23117522
[Journal Article] Zinc(II)-cyclen polyacrylamide gel electrophoresis for detection of mutations in short Ade/Thy-rich DNA fragments2011
- Author(s)
  Kinoshita-Kikuta, E., Eiji Kinoshita, Harada, N., and Koike, T
- Journal Title
  
  Anal. Biochem
  
  Volume: 408 Issue: 2 Pages: 348-350
- DOI
  10.1016/j.ab.2010.09.003
- NAID
  120002753732
- URL
  http://ir.lib.hiroshima-u.ac.jp/00030873
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22590037, KAKENHI-PROJECT-22790037
[Journal Article] A novel phosphate-affinity bead with immobilized Phos-tag for separation and enrichment of phosphopeptides and phosphoproteins2011
- Author(s)
  Kinoshita-Kikuta, E., Yamada, A., Inoue, C., Eiji Kinoshita, and Koike, T
- Journal Title
  
  J. Integrated OMICS
  
  Volume: 1 Issue: 1 Pages: 157-169
- DOI
  10.5584/jiomics.v1i1.46
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22590037, KAKENHI-PROJECT-22790037
[Journal Article] Zinc(II)-cyclen polyacrylamide gel electrophoresis for detection of mutations in short Ade/Thy-rich DNA fragments.2011
- Author(s)
  Kinoshita-Kikuta, E.
- Journal Title
  
  Anal.Biochem.
  
  Volume: 408 Pages: 348-350
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] Improved Phos-tag SDS-PAG Eunder neutral pH conditions for advancedprotein phosphorylation profiling2011
- Author(s)
  Kinoshita, E. and Kinoshita-Kikuta E
- Journal Title
  
  Proteomics
  
  Volume: 11 Pages: 319-323
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] A phos-tag-based fluorescence resonance energy transfer system for theanalysis of the kinase reaction of asubstrate peptide2011
- Author(s)
  Somura, M., Takiyama, K., Kinoshita-Kikuta E., Kinoshita, E., Koike, T
- Journal Title
  
  Analytical Methods
  
  Volume: 3 Pages: 1303-1309
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] Zinc(II). cyclen polyacrylamide gel electrophoresis for detection of mutations in short Ade/ Thy-rich DNA fragments2011
- Author(s)
  Kinoshita E., Kinoshita-Kikuta E., HaradaN., and Koike T
- Journal Title
  
  AnalyticalBiochemistry
  
  Volume: 408 Pages: 348-350
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] A Phos-tag-based fluorescence resonance energy transfer system for the analysis of the kinase reaction of a substrate peptide2011
- Author(s)
  M. Somura, K. Takiyama, E. Kinoshita- Kikuta, E. Kinoshita, and T. Koike
- Journal Title
  
  Analytical Methods
  
  Volume: 3 Issue: 6 Pages: 1303-1309
- DOI
  10.1039/c1ay05016h
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22390006, KAKENHI-PROJECT-22590037, KAKENHI-PROJECT-22790037, KAKENHI-PUBLICLY-23117522
[Journal Article] A novelphosphate-affinity bead with immobilized Phos-tag for separation and enrichment of phosphopeptides and phosphoproteins2011
- Author(s)
  Kinoshita-Kikuta, E., Yamada, A., Inoue, C., Kinoshita, E., Koike, T
- Journal Title
  
  Journal of Integrated Omics
  
  Volume: 1 Pages: 157-269
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] Improved Phos-tag SDS-PAGE under neutral pH conditions for advancedprotein phosphorylation profiling.2011
- Author(s)
  Kinoshita, E.
- Journal Title
  
  Proteomics
  
  Volume: 11 Pages: 319-323
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] A novel phosphate-affinity bead with immobilized Phos-tag for separationand enrichment of phosphopeptides and phosphoproteins.2011
- Author(s)
  Kinoshita-Kikuta, E.
- Journal Title
  
  J.Integr.Omics
  
  Volume: 1 Pages: 157-169
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] Genotyping and mapping assay of single-nucleotide polymorphisms in CYP3A5 using DNA-binding zinc(II) complexes2010
- Author(s)
  Kinoshita, E.
- Journal Title
  
  Clin.Biochem 43
  
  Pages: 302-306
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] Genotyping and mapping assay of single-nucleotide polymorphisms in CYP3A5 using DNA-binding zinc(II) complexes2010
- Author(s)
  K inoshita, E., Emiko Kinoshita-Kikuta, Nakashima, H., Koike, T
- Journal Title
  
  Clin. Biochem 43
  
  Pages: 302-306
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] Monitoring method for thealteration of phosphorylation status in ahigh-molecular-mass protein using Phos-tag electrophoresis technology2010
- Author(s)
  Kinoshita-Kikuta E., Kinoshita E. andKoike T
- Journal Title
  
  Seikagaku
  
  Volume: 82 Pages: 857-862
- Data Source
  KAKENHI-PROJECT-22790037
[Journal Article] Separation and detection of large phosphoproteins using Phos-tag SDS-PAGE2009
- Author(s)
  K inoshita, E., Emiko Kinoshita-Kikuta, Koike, T
- Journal Title
  
  Nat. Protoc 4
  
  Pages: 1513-1521
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] Phos-tag beads as an immunoblotting enhancer for selective detection of phosphoproteins in cell lysates.2009
- Author(s)
  Kinoshita-Kikuta, E.
- Journal Title
  
  Anal.Biochem. 389
  
  Pages: 83-85
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] Mobility shift detection of phosphorylation on large proteins using a Phos-tag SDS-PAGE gel strengthened with agarose.2009
- Author(s)
  Kinoshita, E.
- Journal Title
  
  Proteomics 9
  
  Pages: 4098-4101
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] Mobility shift detection of phosphorylation on large proteins using a Phos-tag SDS-PAGE gel strengthened with agarose2009
- Author(s)
  K inoshita, E., Emiko Kinoshita-Kikuta, Ujihara, H., Koike, T
- Journal Title
  
  Proteomics 9
  
  Pages: 4098-4101
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] Phos-tag beads as an immunoblotting enhancer for selective detection of phosphoproteins in cell lysates2009
- Author(s)
  Emiko Kinoshita-Kikuta, Kinoshita, E., Koike, T
- Journal Title
  
  Anal. Biochem 389
  
  Pages: 83-85
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] A Phos-tag-based fluorescence resonance energy transfer system for the analysis of the dephosphorylation of phosphopeptides2009
- Author(s)
  T akiyama, K., Kinoshita, E., Emiko Kinoshita-Kikuta, Fujioka, Y., Kubo, Y., Koike, T
- Journal Title
  
  Anal. Biochem 388
  
  Pages: 235-241
- NAID
  120002023643
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] Phosphate-affinity gel electrophoresis using a Phos-tag molecule for phosphoproteome study2009
- Author(s)
  K inoshita, E., Emiko Kinoshita-Kikuta, Koike, T
- Journal Title
  
  Curr. Proteomics 6
  
  Pages: 104-121
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] Two-dimensional phosphate-affinity gel electrophoresis for the analysis of phosphoprotein isotypes2009
- Author(s)
  K inoshita, E., Emiko Kinoshita-Kikuta, Matsubara, M., Aoki, Y., Ohie, S., Mouri, Y., Koike, T
- Journal Title
  
  Electrophoresis 30
  
  Pages: 550-559
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] Two-dimensional phosphate-affinity gel electrophoresis for the analysis of phosphoprotein isotypes2009
- Author(s)
  Kinoshita, Eiji
- Journal Title
  
  Electrophoresis 30
  
  Pages: 550-559
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] Separation and detection of large phosphoproteins using Phos-tag SDS-PAGE.2009
- Author(s)
  Kinoshita, E.
- Journal Title
  
  Nat.Protoc. 4
  
  Pages: 1513-1521
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] A mobility shift detection method for DNA methylation analysis using phosphate-affinity polyacrylamide gel electrophoresis2008
- Author(s)
  Emiko Kinoshita-Kikuta, Kinoshita, E., Koike, T
- Journal Title
  
  Anal. Biochem 378
  
  Pages: 102-104
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] Detection of the Gua/Cyt-to-Cyt/Gua mutation in a Gua/Cyt-lined sequence using Zn^<2+>-cyclen polyacrylamide gel electrophoresis2008
- Author(s)
 K inoshita, E., Emiko Kinoshita-Kikuta, Yoshimoto, M., Koike, T
- Journal Title
 
 Anal. Biochem 380
 
 Pages: 122-127
- Peer Reviewed
- Data Source
 KAKENHI-PROJECT-20790036
[Journal Article] Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE2008
- Author(s)
  Kinoshita, Eiji
- Journal Title
  
  Proteomics 8
  
  Pages: 2994-3003
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] A mobility shift detection method for DNA methylation analysis using phosphate-affinity polyacrylamide gelelectrophoresis2008
- Author(s)
  Kinoshita-Kikuta, Emiko
- Journal Title
  
  Anal. Biochem. 378
  
  Pages: 102-104
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] Detection of the Gua/Cyt-to-Cyt/Gua mutation in a Gua/Cyt-lined sequence using Zn^<2 +>-cyclen polyacrylamide gelelectrophoresis2008
- Author(s)
 Kinoshita, Eiji
- Journal Title
 
 Anal. Biochem. 380
 
 Pages: 122-127
- Peer Reviewed
- Data Source
 KAKENHI-PROJECT-20790036
[Journal Article] FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway2008
- Author(s)
  I shiai, M., Kitao, H., Smogorzewska, A., Tomida, J., Kinomura, A., Uchida, E., Saberi, A., Kinoshita, E., Emiko Kinoshita-Kikuta, Koike, T., Tashiro, S., Elledge, S.J., Takata, M
- Journal Title
  
  Nat. Struct. Mol. Biol 15
  
  Pages: 1138-1146
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE2008
- Author(s)
  K inoshita, E., Emiko Kinoshita-Kikuta, Matsubara, M., Yamada, S., Nakamura, H., Shiro, Y., Aoki, Y., Okita, K., Koike, T
- Journal Title
  
  Proteomics 8
  
  Pages: 2994-3003
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20790036
[Journal Article] A mobility-shift detection method for DNA methylation analysis using phosphate-affinity polyacrylamide gel electrophoresis2008
- Author(s)
  Kinoshita-Kikuta, E.
- Journal Title
  
  Anal. Biochem. (印刷中)
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-18790120
[Journal Article] リン酸基アフイニテイー電気泳動法を利用した遺伝子診断法2007
- Author(s)
  木下英司
- Journal Title
  
  実験医学 25
- Data Source
  KAKENHI-PROJECT-18790120
[Journal Article] キナーゼプロフアイリングのための新しいリン酸アフィニティー電気泳勤法2007
- Author(s)
  木下英司
- Journal Title
  
  生物物理化学 51
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-18790120
[Journal Article] Label-free kinase profiling using phosphate affinity polyacrylamide gel electrophoresis2007
- Author(s)
  Emiko Kinoshita-Kikuta
- Journal Title
  
  Molecular ＆ Cellular Proteomics 6
  
  Pages: 356-366
- NAID
  120000878140
- Data Source
  KAKENHI-PROJECT-18790120
[Journal Article] リン酸基アフイニテイー電気泳勤法を利用した細胞内タンパク質リン酸化反応の解析法2007
- Author(s)
  木下英司
- Journal Title
  
  実験医学 25
- Data Source
  KAKENHI-PROJECT-18790120
[Journal Article] A single nucleotide polymorphism genotyping method using phosphate-affinity polyacrylamide gel electrophoresis2007
- Author(s)
  Eiji Kinoshita
- Journal Title
  
  Analytical Biochemistry 361
  
  Pages: 294-298
- Data Source
  KAKENHI-PROJECT-18790120
[Journal Article] 金属イオンの特性を利用したリン酸化タンパク質の特異的認識と検出2007
- Author(s)
  木下英司
- Journal Title
  
  薬学雑誌 127
- NAID
  110006546566
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-18790120
[Journal Article] リン酸基親和性クロマトグラフィーを用いた細胞内のリン酸化タンパク質の網羅的精製2007
- Author(s)
  木下英司
- Journal Title
  
  バイオテクノロジージャーナル 7巻・2号
  
  Pages: 217-220
- Data Source
  KAKENHI-PROJECT-18790120
[Journal Article] Phos-tagを用いたリン酸化タンパク質の検出法 (2)アクリルアミド化Phos-tagを用いたリン酸アフィニティー電気泳動2006
- Author(s)
  木下恵美子
- Journal Title
  
  実験医学 24巻・17号
  
  Pages: 2649-2654
- Data Source
  KAKENHI-PROJECT-18790120
[Journal Article] Phos-tagを用いたリン酸化タンパク質の検出法 (1)ビオチン化Phos-tagを用いたケミルミ検出2006
- Author(s)
  木下英司
- Journal Title
  
  実験医学 24巻・16号
  
  Pages: 2523-2528
- Data Source
  KAKENHI-PROJECT-18790120
[Journal Article] Enrichment of phosphorylated proteins from cell lysate using a novel phosphate-affinity chromatography at physiological pH2006
- Author(s)
  Emiko Kinoshita-Kikuta
- Journal Title
  
  Proteomics 6
  
  Pages: 5088-5095
- Data Source
  KAKENHI-PROJECT-18790120
[Presentation] 新規ヒスチジンキナーゼ阻害剤スクリーニングのためのイムノドットブロットアッセイ2021
- Author(s)
  丸田志乃、木下恵美子、木下英司、小池透
- Organizer
  第71回日本電気泳動学会総会
- Data Source
  KAKENHI-PROJECT-21K06455
[Presentation] E. coli タンパク質共発現系を利用したチロシンキナーゼアッセイ2021
- Author(s)
  木下恵美子、木下英司、小池透
- Organizer
  62回　日本生化学会　中国・四国支部例会
- Data Source
  KAKENHI-PROJECT-21K06455
[Presentation] E. coli タンパク質共発現系を利用したチロシンキナーゼアッセイ2021
- Author(s)
  木下恵美子、木下英司、小池透
- Organizer
  第71回日本電気泳動学会総会
- Data Source
  KAKENHI-PROJECT-21K06455
[Presentation] Phos-tag を用いたリン酸化タンパク質の解析2021
- Author(s)
  木下恵美子
- Organizer
  日本薬学会第141年会
- Data Source
  KAKENHI-PROJECT-18K06596
[Presentation] 無細胞タンパク質合成系で作成したSrcファミリーチロシンキナーゼのリン酸化状態と活性2021
- Author(s)
  木下恵美子、木下英司、小池透
- Organizer
  94回　日本生化学会　　2021,11/3-5
- Data Source
  KAKENHI-PROJECT-21K06455
[Presentation] 無細胞タンパク質合成系で作成したSrcファミリーチロシンキナーゼのリン酸化状態と活性2021
- Author(s)
  木下恵美子、木下英司、小池透
- Organizer
  第71回日本電気泳動学会シンポジウム
- Data Source
  KAKENHI-PROJECT-21K06455
[Presentation] 大腸菌の共発現システムを利用したABLチロシンキナーゼアッセイ2021
- Author(s)
  吉本萌々香、矢野万莉奈、木下恵美子、木下英司、小池透
- Organizer
  第71回日本電気泳動学会総会
- Data Source
  KAKENHI-PROJECT-21K06455
[Presentation] チロシンキナーゼ Lyn は細胞膜への輸送の過程でゴルジ体においてcasein kinase 1γによってセリン13をリン酸化される2020
- Author(s)
  木下恵美子，内海俊彦，木下英司、小池透
- Organizer
  第９３回日本生化学会
- Data Source
  KAKENHI-PROJECT-18K06596
[Presentation] 蛍光Phos-tag誘導体によるブロット膜上のリン酸化タンパク質の定量解析法の開発2020
- Author(s)
  赤山圭佑，木下恵美子，木下英司，小池透
- Organizer
  第９３回日本生化学会
- Data Source
  KAKENHI-PROJECT-18K06596
[Presentation] Phos-tag 蛍光ゲル染色剤を用いた細菌の２成分伝達系のHis/Asp定量的リン酸化解析2020
- Author(s)
  斧尚吾，木下恵美子，木下英司，小池透
- Organizer
  第９３回日本生化学会
- Data Source
  KAKENHI-PROJECT-18K06596
[Presentation] 細胞内のタンパク質のN-ミリストイル化に依存したリン酸化反応2020
- Author(s)
  木下恵美子，木下英司，内海俊彦，小池透
- Organizer
  第７１回日本電気泳動学会総会
- Data Source
  KAKENHI-PROJECT-18K06596
[Presentation] 錯体医薬における亜鉛酵素阻害剤の親和性解析2019
- Author(s)
  草本寛，木下恵美子，木下英司，小池透
- Organizer
  医療薬学フォーラム2019/第27回クリニカルファーマシーシンポジウム
- Data Source
  KAKENHI-PROJECT-18K06596
[Presentation] Phos-tag を用いたリン酸化タンパク質の解析法2019
- Author(s)
  木下恵美子
- Organizer
  日本プロテオーム学会JPrOS2019，日本電気泳動学会JES2019合同年会
- Data Source
  KAKENHI-PROJECT-18K06596
[Presentation] リン酸化タンパク質選択的な電気泳動ゲル蛍光染色法2019
- Author(s)
  草本寛，木下恵美子，木下英司，小池透
- Organizer
  日本プロテオーム学会JPrOS2019，日本電気泳動学会JES2019合同年会
- Data Source
  KAKENHI-PROJECT-18K06596
[Presentation] Phos-tag蛍光ゲル染色剤を用いた２成分系シグナリングの定量モニタリング2019
- Author(s)
  木下英司，木下恵美子，江口陽子，五十嵐雅之，岡島俊英，内海龍太郎，小池透
- Organizer
  第42回日本分子生物学会年会
- Data Source
  KAKENHI-PROJECT-18K06596
[Presentation] 蛍光クエンチングを用いるアルカリホスファターゼ分析の新規蛍光分析法の開発2018
- Author(s)
  芝晃生，木下恵美子，木下　英司，小池透
- Organizer
  日本薬学会第138年会　金沢
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] Phos-tag SDS-PAGEを用いたN-ミリストイル化依存的なタンパク質リン酸化反応の解析2018
- Author(s)
  谷川綾音，木下恵美子，黄波戸亜哉，守屋康子，木下英司，小池透，内海俊彦
- Organizer
  日本農芸化学会大会
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] 亜鉛酵素モデルに対するリガンドの親和性を解析する新規分光分析法の開発2018
- Author(s)
  草本寛，芝晃生，常弘昌弥，藤岡晴人，木下恵美子，木下英司，小池透
- Organizer
  日本薬学会第138年会
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] TAMRA-Phostagを用いたリン酸化タンパク質のゲル染色法2018
- Author(s)
  草本寛，西村朋世，木下恵美子，木下英司，小池透
- Organizer
  第69回日本電気泳動学会
- Data Source
  KAKENHI-PROJECT-18K06596
[Presentation] TAMRA-Phostagを用いたリン酸化タンパク質のゲル染色法2018
- Author(s)
  草本寛，西村朋世，木下恵美子，木下英司，小池透
- Organizer
  第91回日本生化学会大会
- Data Source
  KAKENHI-PROJECT-18K06596
[Presentation] N-ミリストイル化依存的なタンパク質リン酸化反応のPhos-tag SDS-PAGEを用いた解析法の確立2018
- Author(s)
  谷川綾音、木下恵美子、黄波戸亜哉、守屋康子、木下英司、小池透、内海俊彦
- Organizer
  第５９回日本生化学会　中国四国支部例会
- Data Source
  KAKENHI-PROJECT-18K06596
[Presentation] Phos-tagを基盤としたin vitro キナーゼアッセイ法：宿主細胞に由来する偽陽性のキナーゼ活性を見極める有効手段2017
- Author(s)
  木下英司，木下恵美子，小池透
- Organizer
  2017年度生命科学系学会合同年会 ComBio2017
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] プロテインキナーゼのリン酸化動態のプロファイリング法の開発2017
- Author(s)
  上里裕樹，木下英司，木下恵美子，小池透，亀下勇，杉山康憲
- Organizer
  2017年度生命科学系学会合同年会 ComBio2017
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] タンパク質のリン酸化分子種全体を見える化するPhos-tag SDS-PAGE2017
- Author(s)
  木下恵美子
- Organizer
  日本プロテオーム学会2017年大会
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] Phos-tag SDS-PAGEを基盤としたin vitro キナーゼアッセイ法：宿主細胞に由来する偽陽性のキナーゼ活性を見極める2017
- Author(s)
  藤田萌々子，木下恵美子，木下英司，小池透
- Organizer
  第６８回日本電気泳動学会
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] Phos-tag SDS-PAGEの移動度に影響を及ぼすリン酸基以外の要因2017
- Author(s)
  木下恵美子，山戸護久，西山敦洋，河野敏己，木下英司，小池透
- Organizer
  第６８回日本電気泳動学会
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] マルチPK抗体とPhos-tag 2D-PAGEを組み合わせたプロテインキナーゼのプロファイル法2017
- Author(s)
  上里裕樹, 木下英司, 木下恵美子, 小池透,亀下勇, 杉山康憲
- Organizer
  第６８回日本電気泳動学会
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] 細胞内情報伝達におけるMEK1リン酸化種の動態解析2017
- Author(s)
  谷口翼，上田爽夏，石山美帆，木下恵美子，木下英司，小池透
- Organizer
  第６８回日本電気泳動学会
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] フォスタグ技術とプロテオミクス情報を活用した細胞内MEK1リン酸化ダイナミクスのプロファイリング2017
- Author(s)
  木下英司，木下恵美子，小池透
- Organizer
  第137回日本薬学会年会
- Place of Presentation
  仙台市
- Year and Date
  2017-03-24
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] ハイブリッドヒスチジンキナーゼにおけるリン酸基転移反応様式の検証2017
- Author(s)
  池上まどか，木下恵美子，木下英司，小池透
- Organizer
  第６８回日本電気泳動学会
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] Phos- tag SDS- PAGEを用いたリン酸化阻害物質の探索2016
- Author(s)
  曽根雄太郎，山野喜，志賀もえみ，杉本幸子，木下英司，木下恵美子，小池透，大塚英昭，松浪勝義
- Organizer
  日本薬学会第136年会
- Place of Presentation
  横浜市
- Year and Date
  2016-03-26
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] Thio- tag Tipを用いたチオール基含有化合物の選択的分離精製法2016
- Author(s)
  草本寛，藤岡晴人，是竹紀尚，木下恵美子，木下英司，小池透
- Organizer
  日本薬学会第136年会
- Place of Presentation
  横浜市
- Year and Date
  2016-03-26
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] Thio-tag Tipを用いたチオール基含有化合物の選択的分離精製法2016
- Author(s)
  草本寛，芝晃生，是竹紀尚，木下恵美子，木下英司，小池透
- Organizer
  日本プロテオーム学会2016年大会
- Place of Presentation
  北里大学（東京都港区）
- Year and Date
  2016-07-28
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] Phosphate-affinity chromatographic micro-tip technology for enrichment of phosphopeptides towards phosphoproteomic study,2016
- Author(s)
  Maho Kawaguchi, Elena Tianfei Yuan, Yoko Ino, Yayoi Kimura, Hisashi Hirano, Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike,
- Organizer
  15th Human Proteome Organization World Congress
- Place of Presentation
  台北市（台湾）
- Year and Date
  2016-09-18
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] 大腸菌ハイブリッドセンサーキナーゼにおけるリン酸基転移反応様式の検証2016
- Author(s)
  木下英司，木下恵美子，江口陽子，小池透
- Organizer
  第89回日本生化学大会
- Place of Presentation
  仙台市
- Year and Date
  2016-09-25
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] ハイブリッドセンサーキナーゼにおけるリン酸基転移反応の制御機構～レシーバードメインによるリン酸化レベルの負の制御機能～2016
- Author(s)
  木下英司，木下恵美子，小池透
- Organizer
  日本薬学会第136年会
- Place of Presentation
  横浜市
- Year and Date
  2016-03-26
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] チオール含有生体分子を分離・濃縮するためのThio-tag磁気ビーズの開発2016
- Author(s)
  河口真歩，藤岡晴人，木下恵美子，木下英司，小池透
- Organizer
  第67回日本電気泳動学会総会
- Place of Presentation
  釧路市
- Year and Date
  2016-08-26
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] フォスタグ技術によるセンサーキナーゼの自己リン酸化制御機構の解析2016
- Author(s)
  木下恵美子
- Organizer
  第89回日本生化学大会
- Place of Presentation
  仙台市
- Year and Date
  2016-09-25
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] Thio-tag Tipを用いたチオール基含有化合物の選択的分離精製法2016
- Author(s)
  草本寛，藤岡晴人，芝晃生，是竹紀尚，木下恵美子，木下英司，小池透
- Organizer
  第67回日本電気泳動学会総会
- Place of Presentation
  釧路市
- Year and Date
  2016-08-26
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] フォスタグ技術とプロテオミクス情報を活用した細胞内MEK1リン酸化ダイナミクスのプロファイリング2016
- Author(s)
  木下英司，木下恵美子，久保田裕二，武川睦寛，小池透
- Organizer
  第39回日本分子生物学会年会
- Place of Presentation
  横浜市
- Year and Date
  2016-11-30
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] フォスタグ技術を用いたヒスチジンキナーゼにおける自己リン酸化ダイナミクスのプロファイリング2016
- Author(s)
  木下英司，木下恵美子，小池透
- Organizer
  日本プロテオーム学会2016年大会
- Place of Presentation
  北里大学（東京都港区）
- Year and Date
  2016-07-28
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] Thio-tag tip method by using zinc(II)-cyclen-attached agarose beads for enrichment of cysteine-containing biomolecules2016
- Author(s)
  Hiroshi Kusamoto, Akio Shiba, Norinao Koretake, Emiko Kinoshita-Kikuta, Eiji Kinoshita, Tohru Koike
- Organizer
  15th Human Proteome Organization World Congress
- Place of Presentation
  台北市（台湾）
- Year and Date
  2016-09-18
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] Phos-tag SDS-PAGE methodology that effectively uses phosphoproteomic data for profiling the phosphorylation dynamics of MEK12016
- Author(s)
  Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike
- Organizer
  15th Human Proteome Organization World Congress
- Place of Presentation
  台北市（台湾）
- Year and Date
  2016-09-18
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] Phos- tag SDS- PAGEゲルからの標的リン酸化タンパク質の転写効率の改善2015
- Author(s)
  木下英司、草本寛、木下恵美子、小池透
- Organizer
  日本電気泳動学会第66回総会
- Place of Presentation
  東京
- Year and Date
  2015-09-04
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] Phos-tag tip, a novel tool for phosphoproteome study2015
- Author(s)
  EIji Kinoshita, Emiko Kinoshita-Kikuta, Yoko Ino, Yayoi Kimura, Hisashi Hirano, Thoru Koike
- Organizer
  日本プロテオーム学会2015年大会
- Place of Presentation
  熊本市
- Year and Date
  2015-07-23
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] Phos-tagビーズを用いたゲル内消化産物からのリン酸化ペプチド濃縮2015
- Author(s)
  井野洋子、木下英司、木下恵美子、小池透，戸田年総，平野久，木村弥生
- Organizer
  日本電気泳動学会第66回総会
- Place of Presentation
  東京
- Year and Date
  2015-09-04
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] リン酸基捕捉能を持つダーククエンチャーを用いた蛍光分析法2015
- Author(s)
  國貞夏実、木下恵美子、木下英司、小池透
- Organizer
  日本電気泳動学会第66回総会
- Place of Presentation
  東京
- Year and Date
  2015-09-04
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] Developmento of Tio-tag magnetic, bead for rapid and selective separation of thiol-containing biomolecules.2015
- Author(s)
  Maho Kawaguchi, Norinao Koretake, Emiko Kinoshita- Kikuta, Eiji Kinoshita, and Tohru Koike
- Organizer
  Pacifichem2015（国際学会）
- Place of Presentation
  ホノルル市(USA)
- Year and Date
  2015-12-15
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] 迅速かつ簡便にリン酸化生体分子を分離精製するためのPhos-tag Tipの開発2015
- Author(s)
  河口真歩、井野洋子、木村弥生、平野久、木下恵美子、木下英司、小池透
- Organizer
  日本電気泳動学会第66回総会
- Place of Presentation
  東京
- Year and Date
  2015-09-04
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] ハイブリッドセンサーキナーゼのリン酸機リレー情報伝達機構におけるレシーバードメインの制御機構2015
- Author(s)
  木下恵美子，木下英司,　江口陽，吉多美祐，山本兼由，内海龍太郎，小池透
- Organizer
  BMB2015
- Place of Presentation
  神戸市
- Year and Date
  2015-12-01
- Data Source
  KAKENHI-PROJECT-15K07887
[Presentation] Phos-tag SDS-PAGEゲルからの標的タンパク質の転写効率を改善させる秘訣2014
- Author(s)
  木下英司,木下恵美子,小池透
- Organizer
  第37回日本分子生物学会年会
- Place of Presentation
  横浜市
- Year and Date
  2014-11-25
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] SuperSep Phos-tag を用いた低分子量リン酸化タンパク質の解析2014
- Author(s)
  脇本理恵子,木下恵美子,木下英司,小池透
- Organizer
  第65回日本電気泳動学会総会
- Place of Presentation
  横浜市
- Year and Date
  2014-10-25
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] 銅および亜鉛キレート形成によるACE阻害剤エナラプリラトの脂溶性増加2014
- Author(s)
  小西華那世,木下恵美子,木下英司,小池透
- Organizer
  第65回日本電気泳動学会総会
- Place of Presentation
  横浜市
- Year and Date
  2014-10-25
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] チオール含有生体分子を分離・濃縮するための亜鉛サイクレン磁気ビーズの開発2014
- Author(s)
  河口真歩,Yuan Tianfei Elena,藤岡晴人,木下恵美子,木下英司,小池透
- Organizer
  日本プロテオーム学会2014年会
- Place of Presentation
  つくば市
- Year and Date
  2014-07-18
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] hos-tag技術の開発とリン酸化プロテオミクスへの応用2011
- Author(s)
  木木下恵美子
- Organizer
  第9回日本プロテオーム学会大会
- Place of Presentation
  朱鷺メッセ,新潟
- Year and Date
  2011-07-29
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] 亜鉛Phos-tag SDS-PAGEによる細胞内キナーゼ/フォスファターゼの動態解析2011
- Author(s)
  木下恵美子
- Organizer
  第84回日本生化学会
- Place of Presentation
  京都市
- Year and Date
  2011-09-24
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] 改良型Phos-tag Biotinを用いたペプチドマイクロアレイ解析による蛋白質リン酸化反応プロファイリング2011
- Author(s)
  木下英司, 木下恵美子, 小池透
- Organizer
  第9回日本プロテオーム学会大会
- Place of Presentation
  朱鷺メッセ,新潟
- Year and Date
  2011-07-29
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] 中性ゲルを用いた改良型Phos-tag SDS-PAGEによるタンパク質リン酸化フォームの詳細な分離解析法2011
- Author(s)
  木下恵美子, 木下英司, 小池透
- Organizer
  第9回日本プロテオーム学会大会
- Place of Presentation
  朱鷺メッセ,新潟
- Year and Date
  2011-07-28
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] Phos-tag技術の開発とリン酸化プロテオミクスへの応用2011
- Author(s)
  木下恵美子
- Organizer
  第9回日本プロテオーム学会
- Place of Presentation
  新潟市(招待講演)
- Year and Date
  2011-07-29
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] 改良型Phos-tag Biotinの開発とその応用2011
- Author(s)
  木下英司, 木下恵美子, 小池透
- Organizer
  第62回日本電気泳動学会総会
- Place of Presentation
  横浜市
- Year and Date
  2011-11-13
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] 亜鉛-Phos-tag SDS-PAGEによる細胞内キナーゼ/フォスファターゼの動態解析2011
- Author(s)
  木下恵美子
- Organizer
  第84回日本生化学大会
- Place of Presentation
  国立京都国際会館
- Year and Date
  2011-09-24
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] 中性ゲルを用いた改良型Phos-tag SDS-PAGEによるタンパク質リン酸化フォームの詳細な分離解析法2011
- Author(s)
  木下恵美子, 木下英司, 小池透
- Organizer
  第9回日本プロテオーム学会
- Place of Presentation
  新潟市
- Year and Date
  2011-07-29
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] 改良型Phos-tag Biotinを用いたペプチドマイクロアレイ解析による蛋白質リン酸化反応プロファイリング2011
- Author(s)
  木下英司, 木下恵美子, 小池透
- Organizer
  第9回日本プロテオーム学会
- Place of Presentation
  新潟市
- Year and Date
  2011-07-29
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] Phos-tag SDS-PAGE systems for phosphorylation profiling of signaling proteins with a wide range of molecular masses under neutral pH conditions2011
- Author(s)
  Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tohru Koike
- Organizer
  第33回日本分子生物学会年会
- Place of Presentation
  横浜市
- Year and Date
  2011-12-16
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] Emiko Kinoshita-Kikuta, and Tohru Koike, Phos-tag SDS-PAGEsystems for phosphorylation profiling ofsignaling proteins with a wide range ofmolecular masses under neutral pHconditions2011
- Author(s)
  Eiji Kinoshita
- Organizer
  第33回日本分子生物学会年会
- Place of Presentation
  パシフィコ横浜
- Year and Date
  2011-12-16
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] Phos-tagBiotinの開発とその応用2011
- Author(s)
  木下英司, 木下恵美子, 小池透
- Organizer
  第62回日本電気泳動学会総会
- Place of Presentation
  横浜市開講記念会館改良型
- Year and Date
  2011-11-13
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] 新規リン酸親和性Phos-tagビーズを用いたリン酸化タンパク質の分離濃縮法2010
- Author(s)
  木下英司, 木下恵美子, 山田篤志, 小池透
- Organizer
  第51回日本生化学会中国・四国支部例会
- Place of Presentation
  山口大学
- Year and Date
  2010-05-14
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] Improved Phos-tag SDS-PAGE under neutral pH conditions for advanced protein phosphorylation profiling2010
- Author(s)
  木下恵美子
- Organizer
  第33回日本分子生物学会・第83回日本生化学学会年会
- Place of Presentation
  神戸国際展示場
- Year and Date
  2010-12-09
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] Phos-tag-based fluorescence resonanceenergy transfer system for the analysis of phosphorylated molecules2010
- Author(s)
  Masao Somura, Kei Takiyama, TomomiNagai, Eiji Kinoshita, EmikoKinoshita-Kikuta, and Tohru Koike
- Organizer
  2010International Chemical Congress of PacificBasin Societies
- Place of Presentation
  Kamehameha Halls, Honolulu, Hawaii, USA.
- Year and Date
  2010-12-16
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] Quantitative analysis of the phosphorylation states in a bacterialtwo-component system using Phos-tag SDS-PAGE2010
- Author(s)
  Shiho Yanagihara, Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike
- Organizer
  2010International ChemicalCongress of Pacific Basin Societies
- Place of Presentation
  Kamehameha Halls, Honolulu, Hawaii
- Year and Date
  2010-12-16
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] DNA結合性亜鉛錯体を用いたCYP3A5遺伝子における一塩基多型の高精度検出法2010
- Author(s)
  木下英司, 木下恵美子, 中島弘美, 小池透
- Organizer
  日本薬学会第130年会
- Place of Presentation
  岡山県桃太郎アリーナ
- Year and Date
  2010-03-29
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] Phos-tagSDS-PAGE for protein phosphorylationprofiling2010
- Author(s)
  Eiji Kinoshita, Emiko Kinoshita-Kikuta, Saori Okatani, and Tohru Koike
- Organizer
  2010International ChemicalCongress of Pacific Basin Societies
- Place of Presentation
  Kamehameha Halls, Honolulu, Hawaii, USA
- Year and Date
  2010-12-16
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] 新規リン酸親和性カラムを用いたヌクレオチド類の精製法2010
- Author(s)
  宗村雅男, 木下英司, 木下恵美子, 山田篤志, 小池透
- Organizer
  日本薬学会第130年会
- Place of Presentation
  岡山県桃太郎アリーナ
- Year and Date
  2010-03-29
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] Improved Phos-tag Biotin for advanced profiling of proteinphosphorylation2010
- Author(s)
  Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike
- Organizer
  第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
- Place of Presentation
  神戸国際展示場
- Year and Date
  2010-12-09
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] 高分子量リン酸化蛋白質解析のための改良型Phos-tag電気泳動法2010
- Author(s)
  木下英司, 木下恵美子, 小池透
- Organizer
  第61回日本電気泳動学会総会
- Place of Presentation
  北海道大学
- Year and Date
  2010-09-19
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] A Phos-tag-basedfluorescence resonance energy transfersystem for the analysis of the kinasereaction of a substrate peptide2010
- Author(s)
  Masao Somura, Kei Takiyama, EmikoKinoshita-Kikuta, Eiji Kinoshita, andTohru Koike
- Organizer
  第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
- Place of Presentation
  神戸国際展示場
- Year and Date
  2010-12-09
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] Phos-tag SDS-PAGE for protein phosphorylation profiling2010
- Author(s)
  Kinoshita, E.
- Organizer
  2010 International Chemical Congress of Pacific Basin Societies
- Place of Presentation
  Convention Center, Honolulu, Hawaii, USA
- Year and Date
  2010-12-16
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] 細胞内リン酸化シグナル解析におけるPhos-tag固定化ポリマーの新しい応用法2010
- Author(s)
  木下恵美子
- Organizer
  日本薬学会第130年会
- Place of Presentation
  神戸国際展示場(岡山市)
- Year and Date
  2010-03-29
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] Improved Phos-tag SDS-PAGE under neutral pH conditionsfor advanced protein phosphorylationprofiling2010
- Author(s)
  Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike
- Organizer
  第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
- Place of Presentation
  神戸国際展示場
- Year and Date
  2010-12-09
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] 細胞内リン酸化シグナル解析におけるPhos-tag固定化ポリマーの新しい応用法2010
- Author(s)
  木下恵美子, 木下英司, 山田篤志, 氏原弘洋, 小池透
- Organizer
  日本薬学会第130年会
- Place of Presentation
  岡山県桃太郎アリーナ
- Year and Date
  2010-03-29
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] 新規リン酸親和性Phos-tagビーズを用いたヌクレオチド類の分離精製法2010
- Author(s)
  宗村雅男, 木下英司, 木下恵美子, 山田篤志, 小池透
- Organizer
  第51回日本生化学会中国・四国支部例会
- Place of Presentation
  山口大学
- Year and Date
  2010-05-14
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] Improved Phos-tag Biotin for highly sensitive detection of protein phosphorylation2010
- Author(s)
  木下英司
- Organizer
  日本プロテオーム学会第8回大会
- Place of Presentation
  東京ベイホテル東急
- Year and Date
  2010-07-27
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] 蛋白質リン酸化反応を高感度検出するための改良型Phos-tag Biotin2010
- Author(s)
  木下英司, 木下恵美子, 小池透
- Organizer
  第8回日本プロテオーム学会大会
- Place of Presentation
  東京ベイホテル東急
- Year and Date
  2010-07-27
- Data Source
  KAKENHI-PROJECT-22790037
[Presentation] 蛋白質リン酸化解析のためのリン酸基親和性2次元電気泳動法2009
- Author(s)
  木下英司, 木下恵美子, 小池透
- Organizer
  第60回日本電気泳動学会総会
- Place of Presentation
  松本市Mウィング文化センター
- Year and Date
  2009-09-19
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] 細胞内リン酸化タンパク質を高精度に検出するためのPhos-tagビーズによるサンプル前処理法2009
- Author(s)
  山田篤志, 木下恵美子, 木下英司, 小池透
- Organizer
  第82回日本生化学会大会
- Place of Presentation
  神戸国際展示場
- Year and Date
  2009-10-24
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] リン酸基親和性電気泳動法を用いたヒスチジン・アスパラギンリン酸化タンパク質の定量解析法2009
- Author(s)
  柳原志穂, 木下恵美子, 木下英司, 中神明, 小池透
- Organizer
  第82回日本生化学会大会
- Place of Presentation
  神戸国際展示場
- Year and Date
  2009-10-24
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] リン酸基結合ナノ分子を利用した高感度・高効率なエピジェネティクス変異検出技法の創成2009
- Author(s)
  木下英司, 木下恵美子, 中島弘美, 氏原弘洋, 小池透
- Organizer
  日本薬学会第129年会
- Place of Presentation
  国立京都国際会館
- Year and Date
  2009-03-26
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] 改良型リン酸基親和性電気泳動法による200 kDa以上の高分子量タンパク質のリン酸化異性体解析2009
- Author(s)
  木下恵美子, 木下英司, 山田篤志, 氏原弘洋, 小池透
- Organizer
  第82回日本生化学会大会
- Place of Presentation
  神戸国際展示場
- Year and Date
  2009-10-24
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] ペプチドのリン酸化状態を検出する蛍光共鳴エネルギー移動システムの開発2009
- Author(s)
  石村憲和, 木下英司, 木下恵美子, 小池透
- Organizer
  日本薬学会第129年会
- Place of Presentation
  国立京都国際会館
- Year and Date
  2009-03-26
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] フォスタグ電気泳動法を用いた細菌2成分情報伝達系におけるリン酸化ヒスチジンおよびリン酸化アスパラギン酸蛋白質異性体の同時定量解析2009
- Author(s)
  木下英司, 木下恵美子, 中村寛夫, 城宜嗣, 小池透
- Organizer
  第9回蛋白質科学会
- Place of Presentation
  熊本全日空ホテルニュースカイ
- Year and Date
  2009-05-20
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] 改良型リン酸基親和性電気泳動法による200kDa以上の高分子量タンパク質のリン酸化異性体解析2009
- Author(s)
  木下恵美子
- Organizer
  第82回日本生化学会大会
- Place of Presentation
  神戸国際展示場(神戸市)
- Year and Date
  2009-10-24
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] 改変亜鉛サイクレン電気泳動法を用いたAT含有率が高い短PCR断片のSNP解析2009
- Author(s)
  原田奈生子, 木下英司, 木下恵美子, 小池透
- Organizer
  日本薬学会第129年会
- Place of Presentation
  国立京都国際会館
- Year and Date
  2009-03-26
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] Phos-tagを利用したメチル化DNA検出法の開発2009
- Author(s)
  木下英司, 木下恵美子, 小池透
- Organizer
  第50回日本生化学会中国・四国支部例会
- Place of Presentation
  鳥取市とりぎん文化会館
- Year and Date
  2009-05-16
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] Phos-tag固定化ポリマーを利用したシグナル伝達解析における選択的な蛋白質リン酸化検出のための新技術2009
- Author(s)
  木下英司, 木下恵美子, 小池透
- Organizer
  日本ヒトプロテオーム機構第7回大会
- Place of Presentation
  北里大学薬学部
- Year and Date
  2009-07-28
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] リン酸基アフィニティービーズ"Phos-tag Toyopearl"を用いたリン酸化プロテオーム解析における前処理技術の開発2009
- Author(s)
  山田篤志, 木下英司, 木下恵美子, 小池透
- Organizer
  第9回蛋白質科学会
- Place of Presentation
  熊本全日空ホテルニュースカイ
- Year and Date
  2009-05-20
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] ペプチドのリン酸化/脱リン酸化反応を検出するfret型蛍光センサーの開発2008
- Author(s)
  多幾山敬, 木下英司, 木下恵美子, 小池透
- Organizer
  第49回日本生化学会中国・四国支部例会
- Place of Presentation
  香川県県民ホール
- Year and Date
  2008-05-16
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] リン酸基アフィニティービーズ"Phos-tagTM Toyopearl"を用いたリン酸化ペプチド&タンパク質の分離濃縮2008
- Author(s)
  山田篤志, 木下英司, 木下恵美子, 小池透
- Organizer
  BMB2008
- Place of Presentation
  神戸国際展示場
- Year and Date
  2008-12-12
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] ペプチドのリン酸化状態を検出する蛍光共鳴エネルギー移動システムの開発2008
- Author(s)
  多幾山敬, 木下英司, 木下恵美子, 小池透
- Organizer
  BMB2008
- Place of Presentation
  神戸国際展示場
- Year and Date
  2008-12-12
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] Separation of phosphoprotein isotypes with the same phosphate stoichiometry using phosphate-affinity SDS-PAGE2008
- Author(s)
  木下英司
- Organizer
  BMB2008
- Place of Presentation
  神戸国際展示場
- Year and Date
  2008-12-12
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] フォスタグ結合型新規リン酸基アフィニティービーズを利用した細胞抽出液からのリン酸化蛋白質の分離と濃縮2008
- Author(s)
  木下英司, 木下恵美子, 山田篤志, 井上知香, 小池透
- Organizer
  日本ヒトプロテオーム機構第6回大会
- Place of Presentation
  ホテル阪急エキスポパーク
- Year and Date
  2008-07-29
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] リン酸基親和性電気泳動法を利用した新しい遺伝子診断技術2008
- Author(s)
  木下英司, 木下恵美子, 小池透
- Organizer
  第59回日本電気泳動学会総会
- Place of Presentation
  麻布大学百周年記念ホール
- Year and Date
  2008-11-16
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] リン酸基親和性電気泳動法を利用した同じリン酸化量を有するリン酸化蛋白質異性体の分離検出2008
- Author(s)
  木下英司, 木下恵美子, 松原守, 山田斉爾, 中村寛夫, 城宜嗣, 青木悠里, 沖田鋼季, 小池透
- Organizer
  BMB2008
- Place of Presentation
  神戸国際展示場
- Year and Date
  2008-12-12
- Data Source
  KAKENHI-PROJECT-20790036
[Presentation] 遺伝子変異を認識する機能性分子を用いた遺伝子診断技術の開発2007
- Author(s)
  木下英司
- Organizer
  日本電気泳動学会
- Place of Presentation
  宇部全日空ホテル
- Year and Date
  2007-11-09
- Data Source
  KAKENHI-PROJECT-18790120
[Presentation] 亜鉛サイクレン磁気ビーズを用いたチオール化合物の精製
- Author(s)
  藤岡晴人，稗田雄三，常弘昌弥，木下恵美子，木下英司，小池　透
- Organizer
  日本薬学会第134年会
- Place of Presentation
  熊本市総合体育館，熊本市
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] Quantitative analysis of the phosphorylation state of a hybrid histidine kinase in bacterial twocomponent system by using phosphate-affinity SDS-PAGE
- Author(s)
  Keisuke Edahiro, Akio Shiba, Emiko Kinoshita-Kikuta, Yuki Inoue, Eiji Kinoshita, and Tohru Koike
- Organizer
  HUPO 12th Annual World Congress (HUPO2013)
- Place of Presentation
  パシフィコ横浜，横浜市
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] 中性Phos-tag SDS-PAGEを用いたタンパク質リン酸化反応の解析
- Author(s)
  木下恵美子，木下英司，小池　透
- Organizer
  第85回日本生化学会
- Place of Presentation
  福岡国際会議場　マリンメッセ福岡
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] ハイブリッド型ヒスチジンキナーゼにおけるリン酸基転移反応の制御機構解析
- Author(s)
  枝廣圭祐，芝晃生，井上裕希，木下恵美子，木下英司，小池透
- Organizer
  第64回日本電気泳動学会総会
- Place of Presentation
  東北福祉大学ステーションキャンパス，仙台市
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] 迅速かつ簡便にリン酸化生体分子を分離生成するためのPhos-tag磁気ビーズの創製
- Author(s)
  常弘昌弥，目木勇馬，木下恵美子，木下英司，小池　透
- Organizer
  第85回日本生化学会
- Place of Presentation
  福岡国際会議場　マリンメッセ福岡
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] Phos-tag Biotin as an on-demand tool for study on protein phosphorylome.
- Author(s)
  Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike
- Organizer
  HUPO 12th Annual World Congress (HUPO2013)
- Place of Presentation
  パシフィコ横浜，横浜市
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] 迅速かつ簡便にリン酸化生体分子を分離精製するための Phos-tag磁気ビーズの開発
- Author(s)
  常弘昌弥, 目木勇馬, 木下恵美子，木下英司，小池透
- Organizer
  第64回日本電気泳動学会総会
- Place of Presentation
  東北福祉大学ステーションキャンパス，仙台市
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] 迅速かつ簡便にリン酸化生体分子を分離精製するためのPhos-tag磁気ビーズの創製
- Author(s)
  常弘昌弥,　目木勇馬,　木下恵美子，木下英司,　小池　透
- Organizer
  修飾シグナル病　第３回　公開シンポジウム
- Place of Presentation
  東京大学医科学研究所，東京
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] ハイブリッド型ヒスチジンキナーゼにおけるリン酸基転移反応の制御機構解析
- Author(s)
  枝廣圭祐,　芝　晃生,　井上裕希，木下恵美子,　木下英司，小池　透
- Organizer
  修飾シグナル病　第３回　公開シンポジウム
- Place of Presentation
  東京大学医科学研究所，東京
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] 中性亜鉛Phos-tagプレキャストゲル（　SuperSep Phos-tag　）を用いたリン酸化タンパク質の解析
- Author(s)
  木下恵美子，木下英司，小池　透
- Organizer
  第63回日本電気泳動学会
- Place of Presentation
  沖縄コンベンションセンター
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] Quantitative analysis of effects of pH on the stability of His- and Asp-phosphoproteins in bacterial two-component system by using phosphate-affinity SDS-PAGE
- Author(s)
  Akio Shiba, Keisuke Edahiro, Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike
- Organizer
  HUPO 12th Annual World Congress (HUPO2013)
- Place of Presentation
  パシフィコ横浜，横浜市
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] 中性Phos-tag SDS-PAGEを用いたタンパク質リン酸化反応の解析
- Author(s)
  木下恵美子，木下英司，小池　透
- Organizer
  第10回日本プロテオーム学会大会
- Place of Presentation
  東京科学未来館
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] Rapid and selective separation of phosphorylated biomolecules by using a Phos-tag-based magneticbead method
- Author(s)
  Masaya Tsunehiro, Yuma Meki, Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike
- Organizer
  HUPO 12th Annual World Congress (HUPO2013)
- Place of Presentation
  パシフィコ横浜，横浜市
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] 中性Phos-tag SDS-PAGEを用いたヒスチジン，アスパラギン酸リン酸化タンパク質の解析
- Author(s)
  芝　晃生，枝廣　圭祐，木下恵美子，木下英司，小池　透
- Organizer
  第85回日本生化学会
- Place of Presentation
  福岡国際会議場　マリンメッセ福岡
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] Phosphorylation profiling of MAPK signaling cascade proteins by using neutral Phos-tag SDS-PAGE
- Author(s)
  Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike
- Organizer
  HUPO 12th Annual World Congress (HUPO2013)
- Place of Presentation
  パシフィコ横浜，横浜市
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] ヒスチジン，アスパラギン酸のタンパク質リン酸化解析におけるチオリン酸基供与体(ATP-gammaS)利用の有用性の検討
- Author(s)
  芝晃生，枝廣圭祐，井上裕希，木下恵美子，木下英司，小池透
- Organizer
  第64回日本電気泳動学会総会
- Place of Presentation
  東北福祉大学ステーションキャンパス，仙台市
- Data Source
  KAKENHI-PROJECT-24590050
[Presentation] 近接Phos-tagゲルを用いたリン酸化タンパク質の電気泳動法
- Author(s)
  目木勇馬，木下恵美子，木下英司，小池透
- Organizer
  第64回日本電気泳動学会総会
- Place of Presentation
  東北福祉大学ステーションキャンパス，仙台市
- Data Source
  KAKENHI-PROJECT-24590050

1. 武川睦寛

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
2. 小池透

# of Collaborated Projects: 0 results

# of Collaborated Products: 11 results
3. 木下英司

# of Collaborated Projects: 0 results

# of Collaborated Products: 8 results
4. 水谷秀樹

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
5. 木村弥生

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
6. 江口陽子

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Kinoshita Emiko 木下 恵美子

KINOSHITA Emiko 木下 恵美子

Research Projects

Research Products

Co-Researchers

病原菌のリン酸化酵素阻害剤（抗生物質）の多検体高速スクリーニングシステムの開発Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Generating proteomic data on signalling pathways in pathogenic bacteria for the discovery of new antibiotic target molecules.Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Development of a system for profiling the pathophysiology of diseases from the phosphorylated states of proteinsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Creation of the analysis technique to visualize the dynamics of labile intermediates in phosphotransfer reactionsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Development of phosphate affinity electrophoresis system for phosphorylation profiling of proteins with a wide range of molecular masses.Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

A method for simultaneous detection of dual post-translational modifications using phosphate-affinity electrophoresisPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Development of phosphate-affinity two-dimensional fluorescence difference gel electrophoresisPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

オーダーメード医療を目的とした迅速かつ網羅的な疾患遺伝子多型検出法の開発Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Book] Neuroproteomics: Neuromthods, 146 (ed. by Li, KW)2019

Author(s)

Total Pages

Publisher

ISBN

Data Source

[Book] Phosphopeptide detection with biotin- labeled Phos- tag, in Phospho- proteomics: Methods and Protocols, Methods in Molecular Biology, 13552016

Author(s)

Total Pages

Publisher

Data Source

[Book] Phis-Tag based affinity chromatography techniques for enrichment of the phosphoproteome, in Protein Modifications in Pathogenic Dysregulation of Signaling.2015

Author(s)

Total Pages

Publisher

Data Source

[Book] Phos- tag technology for kinomics, in Kinomics: Approaches and Applications.2015

Author(s)

Total Pages

Publisher

Data Source

[Book] Neutral phosphate affinity SDS- PAGE system for profiling of protein phosphorylation, in Proteomic Profiling: Methods and Protocols,Methods in Molecular Biology, 12952015

Author(s)

Total Pages

Publisher

Data Source

[Book] DNA Replication and Mutation2012

Kinoshita Emiko 木下恵美子

KINOSHITA Emiko 木下恵美子

[Journal Article] Crystal Structure of Bis{1,3-bis[bis(pyridin-2-ylmethyl)amino]propan-2-olato-dizinc(II)}orthophosphate Tris(perchlorate) Octahydrate, [(Phos-tag)<sub>2</sub>-PO<sub>4</sub><sup>3−</sup>][ClO<sub>4</sub><sup>−</sup>]<sub>3</sub>·8H<sub>2</sub>O2021