Hosaka Toshiaki 保坂俊彰

Researcher Number	40462725
Other IDs	https://orcid.org/0000-0002-7652-8114
Affiliation (Current)	2025: 国立研究開発法人理化学研究所, 生命医科学研究センター, 技師
Affiliation (based on the past Project Information) *help	2020 – 2024: 国立研究開発法人理化学研究所, 生命機能科学研究センター, 技師 2018 – 2020: 国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 2015 – 2017: 国立研究開発法人理化学研究所, ライフサイエンス技術基盤研究センター, 研究員
Review Section/Research Field	Principal Investigator Complex systems / Basic Section 43030:Functional biochemistry-related / Structural biochemistry / Biophysics Except Principal Investigator Basic Section 44030:Plant molecular biology and physiology-related / Medium-sized Section 43:Biology at molecular to cellular levels, and related fields / Basic Section 43040:Biophysics-related / Bio-related chemistry
Keywords	Principal Investigator X線結晶構造解析 / 大腸菌無細胞合成系 / 時分割結晶構造解析 / SACLA / ケージド化合物 / クロライドイオンポンプ / クロライドイオンポンプロドプシン / 光異性化アミノ酸 / 非天然型アミノ酸 / ミトコンドリア … More / 複合体 / クライオ電子顕微鏡 / TSPO / VDAC / 非天然アミノ酸 / 結晶構造解析 / イオンポンプ / クロライドポンプ / 膜タンパク質 / X線自由電子レーザー / 時分割構造解析 / ロドプシン … More Except Principal Investigator X線自由電子レーザー / 情報伝達 / タンパク質相互作用 / 結晶構造解析 / 二酸化炭素 / 気孔 / 動的構造 / 時分割結晶構造解析 / 微生物型ロドプシン / X線結晶解析 / ロドプシン / 生体材料 / 可溶化 / 界面活性剤 / 両親媒性ペプチド / 脂質二分子膜 / リン脂質 / 界面活性型ペプチド / 膜タンパク質 / ナノディスク Less

細胞膜受容体の動的構造解析と活性化機構の解明
- Principal Investigator
  
  南後恵理子
- Project Period (FY)
  2024 – 2026
- Research Category
  
  Grant-in-Aid for Scientific Research (A)
- Review Section
  
  Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
- Research Institution
  Tohoku University
多角的構造解析による植物の二酸化炭素感知機構の解明
- Principal Investigator
  
  高橋洋平
- Project Period (FY)
  2023 – 2025
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 44030:Plant molecular biology and physiology-related
- Research Institution
  Nagoya University
多様なミトコンドリア膜タンパク質VDAC複合体の構造解析Principal Investigator
- Principal Investigator
  
  保坂俊彰
- Project Period (FY)
  2022 – 2025
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 43030:Functional biochemistry-related
- Research Institution
  Institute of Physical and Chemical Research
大腸菌無細胞合成系を利用した光反応性非天然型アミノ酸導入タンパク質調製法の開発Principal Investigator
- Principal Investigator
  
  保坂俊彰
- Project Period (FY)
  2022 – 2023
- Research Category
  
  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
- Review Section
  
  Complex systems
- Research Institution
  Institute of Physical and Chemical Research
Time-resolved X-ray crystallography of light-driven ion pumps and their applications
- Principal Investigator
  
  Nango Eriko
- Project Period (FY)
  2021 – 2023
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Review Section
  
  Basic Section 43040:Biophysics-related
- Research Institution
  Tohoku University
大腸菌無細胞合成系を利用した時分割SFX実験に適したサンプル調製法の開発Principal Investigator
- Principal Investigator
  
  保坂俊彰
- Project Period (FY)
  2020 – 2021
- Research Category
  
  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
- Review Section
  
  Complex systems
- Research Institution
  Institute of Physical and Chemical Research
Time-resolved serial femtosecond crystallography at an XFEL to reveal conformational changes in Cl- pump rhodopsin, NM-R3Principal Investigator
- Principal Investigator
  
  Hosaka Toshiaki
- Project Period (FY)
  2017 – 2020
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Structural biochemistry
- Research Institution
  Institute of Physical and Chemical Research
Preparation of novel nanodiscs that allow to in site analysis of membrane proteins
- Principal Investigator
  
  IMURA TOMOHIRO
- Project Period (FY)
  2016 – 2018
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Bio-related chemistry
- Research Institution
  National Institute of Advanced Industrial Science and Technology
Analysis of intermediate structure in proton pump mechanism of proteorhodopsinPrincipal Investigator
- Principal Investigator
  
  Hosaka Toshiaki
- Project Period (FY)
  2015 – 2016
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Biophysics
- Research Institution
  Institute of Physical and Chemical Research

All 2022 2019 2017 2016

All Journal Article Presentation Book

[Book] Advanced Methods in Structural Biology2016
- Author(s)
  T. Kimura-Someya, T. Hosaka, T. Shinoda, K. Shimono, M. Shirouzu, S. Yokoyama
- Total Pages
  340
- Publisher
  Springer
- Data Source
  KAKENHI-PROJECT-15K18523
[Journal Article] Conformational alterations in unidirectional ion transport of a light-driven chloride pump revealed using X-ray free electron lasers.2022
- Author(s)
  Hosaka, T. et al.
- Journal Title
  
  Proc. Natl. Acad. Sci. USA
  
  Volume: 119 Issue: 9
- DOI
  10.1073/pnas.2117433119
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PLANNED-19H05784, KAKENHI-PUBLICLY-20H05450, KAKENHI-PROJECT-18H04136, KAKENHI-PROJECT-19H00923, KAKENHI-PROJECT-22H00557, KAKENHI-PROJECT-21H02439, KAKENHI-ORGANIZER-19H05776, KAKENHI-PLANNED-19H05781, KAKENHI-PLANNED-19H05777
[Journal Article] A distinct lineage of giant viruses brings a rhodopsin photosystem to unicellular marine predators2019
- Author(s)
  Needham David M.、Yoshizawa Susumu、Hosaka Toshiaki et. al.
- Journal Title
  
  Proceedings of the National Academy of Sciences
  
  Volume: 116 Issue: 41 Pages: 20574-20583
- DOI
  10.1073/pnas.1907517116
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-17K07324, KAKENHI-PROJECT-17K08296, KAKENHI-PROJECT-18K19224, KAKENHI-PROJECT-19K16090, KAKENHI-PUBLICLY-19H04727, KAKENHI-PUBLICLY-19H05396, KAKENHI-PROJECT-18H04136, KAKENHI-PROJECT-18H02411, KAKENHI-PROJECT-19J01352, KAKENHI-PROJECT-16H06279
[Journal Article] Crystal structural characterization reveals novel oligomeric interactions of human voltage-dependent anion channel 1.2017
- Author(s)
  Hosaka T, Okazaki M, Kimura-Someya T, Ishizuka-Katsura Y, Ito K, Yokoyama S, Dodo K, Sodeoka M, Shirouzu M.
- Journal Title
  
  Protein Science
  
  Volume: 9 Issue: 9 Pages: 1749-1758
- DOI
  10.1002/pro.3211
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-17K07324, KAKENHI-PROJECT-17K08296
[Journal Article] Structural mechanism for light-driven transport by a new type of chloride ion pump, Nonlabens marinus rhodopsin-32016
- Author(s)
  Toshiaki Hosaka, Susumu Yoshizawa, Yu Nakajima, Noboru Ohsawa, Masakatsu Hato, Edward F. DeLong, Kazuhiro Kogure, Shigeyuki Yokoyama, Tomomi Kimura-Someya, Wataru Iwasaki and Mikako Shirouzu
- Journal Title
  
  Journal of Biological Chemistry
  
  Volume: 291 Issue: 34 Pages: 17488-17495
- DOI
  10.1074/jbc.m116.728220
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15H02800, KAKENHI-PROJECT-15J07201, KAKENHI-PROJECT-15K18523, KAKENHI-PROJECT-16H06279
[Journal Article] A three dimensional movie of structural changes in bacteriorhodopsin.2016
- Author(s)
  Nango, E., Royant, A.†, Kubo, M.† et al. (†equal contribution)(45名中2番目)
- Journal Title
  
  Science
  
  Volume: 354 Issue: 6319 Pages: 1552-1557
- DOI
  10.1126/science.aah3497
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PUBLICLY-15H01055, KAKENHI-PROJECT-15H03841, KAKENHI-PROJECT-15H04338, KAKENHI-PROJECT-15H05476, KAKENHI-PROJECT-15K18486, KAKENHI-PROJECT-15K18523, KAKENHI-PROJECT-16H06315
[Presentation] Structural changes in chloride ion pump rhodopsin by time-resolved crystallography2022
- Author(s)
  保坂俊彰
- Organizer
  大阪大学蛋白研セミナー　Frontier of Dynamic Structural Biology（招待講演）
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-21H02439
[Presentation] Structural changes in chloride ion pump rhodopsin by time-resolved crystallography2022
- Author(s)
  保坂俊彰
- Organizer
  大阪大学蛋白研セミナー　Frontier of Dynamic Structural Biology
- Invited
- Data Source
  KAKENHI-PUBLICLY-22H04759
[Presentation] Cl-イオンポンプロドプシンの時分割XFEL結晶構造解析2019
- Author(s)
  保坂俊彰
- Organizer
  第19回日本蛋白質科学会年会
- Data Source
  KAKENHI-PROJECT-17K07324
[Presentation] Structural changes in Cl- pump rhodopsin by time-resolved serial femtosecond crystallography2019
- Author(s)
  保坂俊彰
- Organizer
  CBI学会2019年大会
- Data Source
  KAKENHI-PROJECT-17K07324
[Presentation] Nonlabens marinus由来新規光駆動性クロライドポンプの結晶構造解析2017
- Author(s)
  保坂俊彰, 吉澤晋, 中島悠, 大沢登, 羽藤正勝, Edward F. DeLong, 木暮一啓, 横山茂之, 染谷友美, 岩崎渉, 白水美香子
- Organizer
  日本蛋白質科学会
- Data Source
  KAKENHI-PROJECT-17K07324

1. Nango Eriko (90376947)

# of Collaborated Projects: 2 results

# of Collaborated Products: 0 results
2. IMURA TOMOHIRO (10371022)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
3. 浅田秀基 (20399041)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
4. 清中茂樹 (90422980)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
5. 熊坂崇 (30291066)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
6. 高橋洋平 (80592964)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
7. YOSHIZAWA Susumu

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
8. 久保稔

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Hosaka Toshiaki 保坂 俊彰

Research Projects

Research Products

Co-Researchers

細胞膜受容体の動的構造解析と活性化機構の解明

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

多角的構造解析による植物の二酸化炭素感知機構の解明

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

多様なミトコンドリア膜タンパク質VDAC複合体の構造解析Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

大腸菌無細胞合成系を利用した光反応性非天然型アミノ酸導入タンパク質調製法の開発Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Time-resolved X-ray crystallography of light-driven ion pumps and their applications

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

大腸菌無細胞合成系を利用した時分割SFX実験に適したサンプル調製法の開発Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Time-resolved serial femtosecond crystallography at an XFEL to reveal conformational changes in Cl- pump rhodopsin, NM-R3Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Preparation of novel nanodiscs that allow to in site analysis of membrane proteins

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Analysis of intermediate structure in proton pump mechanism of proteorhodopsinPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Book] Advanced Methods in Structural Biology2016

Author(s)

Total Pages

Publisher

Data Source

[Journal Article] Conformational alterations in unidirectional ion transport of a light-driven chloride pump revealed using X-ray free electron lasers.2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] A distinct lineage of giant viruses brings a rhodopsin photosystem to unicellular marine predators2019

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Crystal structural characterization reveals novel oligomeric interactions of human voltage-dependent anion channel 1.2017

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Structural mechanism for light-driven transport by a new type of chloride ion pump, Nonlabens marinus rhodopsin-32016

Author(s)

Hosaka Toshiaki 保坂俊彰