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Puppulin Leonardo  PUPPULIN LEONARDO

ORCIDConnect your ORCID iD *help
Researcher Number 40771580
Other IDs
Affiliation (based on the past Project Information) *help 2018 – 2021: 金沢大学, ナノ生命科学研究所, 特任助教
2017: 京都府立医科大学, 医学部, 研究員
2016: 京都府立医科大学, 医学部, プロジェクト研究員
Review Section/Research Field
Principal Investigator
Basic Section 28040:Nanobioscience-related / General physiology
Keywords
Principal Investigator
Cancer cell / pH / Raman spectroscopy / Imaging / Reactive Oxygen Species / redox biology / nano-sensor / hydrogen peroxides / SERS / nanosensor … More / cell surface pH / cell surface / Hydrogen peroxide / Nanosensors / 間質液pH / MRI / アルツハイマー型認知症 / 糖尿病 / タンパク質 / グルコース / HC03-濃度 / 間質液 / ラマン分光 / 表面強調ラマン分光法 / Gold nanoparticles / gold nanoparticles / interstitial fluid Less
  • Research Projects

    (2 results)
  • Research Products

    (8 results)
  • Co-Researchers

    (10 People)
  •  Nanometer scale resolution imaging of pH and reactive oxygen species on the outer membrane surface for fast and effective cell discrimination in cancer tissues.Principal Investigator

    • Principal Investigator
      Puppulin Leonardo
    • Project Period (FY)
      2019 – 2021
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Review Section
      Basic Section 28040:Nanobioscience-related
    • Research Institution
      Kanazawa University
  •  Development of new methods for measuring ion concentrations in interstitial fluid using optical spectroscopyPrincipal Investigator

    • Principal Investigator
      Puppulin Leonardo
    • Project Period (FY)
      2016 – 2018
    • Research Category
      Grant-in-Aid for Young Scientists (B)
    • Research Field
      General physiology
    • Research Institution
      Kanazawa University
      Kyoto Prefectural University of Medicine

All 2021 2020 2019 2018 2017 2016

All Journal Article Presentation

  • [Journal Article] Plasma membrane anchored nanosensor for quantifying endogenous production of H2O2 in living cells2021

    • Author(s)
      Hosogi Shigekuni、Marunaka Yoshinori、Ashihara Eishi、Yamada Tadaaki、Sumino Ayumi、Tanaka Hideo、Puppulin Leonardo
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 179 Pages: 113077-113077

    • DOI

      10.1016/j.bios.2021.113077

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19K05228, KAKENHI-PROJECT-18K19422, KAKENHI-PROJECT-18H03182
  • [Journal Article] Bioconjugation strategy for cell surface labelling with gold nanostructures designed for highly localized pH measurement.2018

    • Author(s)
      Leonardo Puppulin, Shigekuni Hosogi, Hongxin Sun, Kazuhiko Matsuo, Toshio Inui, Yasuaki Kumamoto, Toshinobu Suzaki, Hideo Tanaka and Yoshinori Marunaka
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 5278-5278

    • DOI

      10.1038/s41467-018-07726-5

    • NAID

      120006549200

    • Peer Reviewed / Open Access / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-17K00613, KAKENHI-PROJECT-18K19459, KAKENHI-PROJECT-18K15983, KAKENHI-PROJECT-18H03182, KAKENHI-PROJECT-16K18990, KAKENHI-PROJECT-16K18991
  • [Journal Article] Raman micro-spectroscopy as a viable tool to monitor and estimate the ionic transport in epithelial cells2017

    • Author(s)
      Puppulin P, Pezzotti G, Sun H, Hosogi S, Nakahari T, Inui T, Kumamoto Y, Tanaka H, Marunaka Y
    • Journal Title

      Scientific Reports

      Volume: 印刷中

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-16K18990
  • [Journal Article] Validation of a protocol based on Raman and Infrared spectroscopies to nondestructively estimate the oxidative degradation of UHMWPE used in total joint arthroplasty2016

    • Author(s)
      Puppulin L, Miura Y, Casagrande E, Hasegawa M, MARUNAKA Y, Tone S, Sudo A, Pezzotti G
    • Journal Title

      Acta Biomaterialia

      Volume: 38 Pages: 167-178

    • DOI

      10.1016/j.actbio.2016.04.040

    • Peer Reviewed / Acknowledgement Compliant / Open Access / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-15K15034, KAKENHI-PROJECT-15K08732, KAKENHI-PROJECT-16K18990
  • [Presentation] Surface enhanced Raman spectroscopy probe for nanometer-scale measurement of pH and hydrogen peroxides on the outer membrane of cells2020

    • Author(s)
      Puppulin Leonardo
    • Organizer
      The 97th Annual Meeting of the Physiological Society of Japan
    • Invited
    • Data Source
      KAKENHI-PROJECT-19K05228
  • [Presentation] Highly localized pH sensing on the outer membrane of cells using surface enhanced Raman spectroscopy2019

    • Author(s)
      Puppulin Leonardo
    • Organizer
      Federation of the Asian and Oceanian Physiological Societies Congress
    • Data Source
      KAKENHI-PROJECT-16K18990
  • [Presentation] Raman spectroscopy as a viable tool to monitor the effect of aldosterone on A6 renal epithelial cell2017

    • Author(s)
      Puppulin L, Pezzotti G, Sun H, Hosogi S, Nakahari T, Kumamoto Y, Tanaka H, Marunaka Y
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      アクトシティ浜松(静岡県・浜松市)
    • Year and Date
      2017-03-28
    • Data Source
      KAKENHI-PROJECT-16K18990
  • [Presentation] New method based on Raman spectroscopy to detect in-situ concentration of HCO3-, Na+ and K+ from physiologically relevant solutions2016

    • Author(s)
      Puppulin L , Pezzotti G, Hosogi S, Nakahari T, Marunaka Y
    • Organizer
      International Symposium on Ion Channels, Transporters and Signal Transduction
    • Place of Presentation
      京都ホテル(京都府・京都市)
    • Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-16K18990
  • 1.  細木 誠之 (30433254)
    # of Collaborated Projects: 2 results
    # of Collaborated Products: 4 results
  • 2.  山田 忠明 (00507048)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 1 results
  • 3.  Matsuo Kazuhiko
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 4.  Marunaka Yoshinori
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 4 results
  • 5.  Inui Toshio
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 1 results
  • 6.  Tanaka Hideo
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 2 results
  • 7.  Kumamoto Yasuaki
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 2 results
  • 8.  Suzaki Toshinobu
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 1 results
  • 9.  Sun Hongxin
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 2 results
  • 10.  PEZZOTTI Giuseppe
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 1 results

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