Shimomura Takushi 下村拓史

Researcher Number	50635464
Other IDs
Affiliation (Current)	2025: 生理学研究所, 分子細胞生理研究領域, 助教
Affiliation (based on the past Project Information) *help	2016 – 2023: 生理学研究所, 分子細胞生理研究領域, 助教
Review Section/Research Field	Principal Investigator Basic Section 48020:Physiology-related / Complex systems / General physiology
Keywords	Principal Investigator 電気生理学 / イオンチャネル / 非天然アミノ酸 / 構造生物学 / 膜タンパク質 / 光生理学 / 時分割構造解析 / 高速分子動画 / two-pore channel / Two-pore Na+ channel … More / 膜電位依存性イオンチャネル / TPC / Na+チャネル / Naチャネル / Caチャネル / イオン選択性 / 電位依存性イオンチャネル / ケミカルバイオロジー / 電位依存性 / 受精 / 電位依存性Na+チャネル / 生体分子 / voltage-gated channel / 光生理学的解析 / Two-Pore channel / 膜電位存性Na+チャネル / ホスホイノシチド / 膜電位依存性 / Nav / Two-pore channel Less

光生理学的手法を用いたTwo pore channelにおけるイオン選択性制御の分子基盤の解明Principal Investigator
- Principal Investigator
  
  下村拓史
- Project Period (FY)
  2023 – 2025
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 48020:Physiology-related
- Research Institution
  National Institute for Physiological Sciences
非天然アミノ酸を用いた汎用かつ簡便なタンパク質光感受化法Principal Investigator
- Principal Investigator
  
  下村拓史
- Project Period (FY)
  2022 – 2023
- Research Category
  
  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
- Review Section
  
  Complex systems
- Research Institution
  National Institute for Physiological Sciences
Unnatural amino acid-based state-specific analysis of ion channelsPrincipal Investigator
- Principal Investigator
  
  Shimomura Takushi
- Project Period (FY)
  2020 – 2022
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 48020:Physiology-related
- Research Institution
  National Institute for Physiological Sciences
光異性化アミノ酸導入による、リガンド依存性イオンチャネルの光制御化法の開発Principal Investigator
- Principal Investigator
  
  下村拓史
- Project Period (FY)
  2020 – 2021
- Research Category
  
  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
- Review Section
  
  Complex systems
- Research Institution
  National Institute for Physiological Sciences
Analysis of the involvment of TPC3 channel for polyspermyPrincipal Investigator
- Principal Investigator
  
  Shimomura Takushi
- Project Period (FY)
  2018 – 2019
- Research Category
  
  Grant-in-Aid for Early-Career Scientists
- Review Section
  
  Basic Section 48020:Physiology-related
- Research Institution
  National Institute for Physiological Sciences
Analysis of the unique voltage-dependent mechanism of a Na+ channel by voltage-clamp fluorometryPrincipal Investigator
- Principal Investigator
  
  Shimomura Takushi
- Project Period (FY)
  2016 – 2017
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  General physiology
- Research Institution
  National Institute for Physiological Sciences

All 2024 2023 2022 2021 2020 2019 2018 2017 2016

All Journal Article Presentation

[Journal Article] Conformational rearrangements in the second voltage sensor domain switch PIP2- and voltage-gating modes in two-pore channels2023
- Author(s)
  Shimomura T, Hirazawa K, Kubo Y
- Journal Title
  
  Proc Natl Acad Sci USA
  
  Volume: 120(6) Issue: 6
- DOI
  10.1073/pnas.2209569120
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20H03424, KAKENHI-PROJECT-20K07284, KAKENHI-PUBLICLY-22H04758
[Journal Article] Dopamine drives neuronal excitability via KCNQ channel phosphorylation for reward behavior2022
- Author(s)
  Tsuboi Daisuke、Otsuka Takeshi、Shimomura Takushi、Faruk Md Omar、Yamahashi Yukie、Amano Mutsuki、Funahashi Yasuhiro、Kuroda Keisuke、Nishioka Tomoki、Kobayashi Kenta、Sano Hiromi、Nagai Taku、Yamada Kiyofumi、Tzingounis Anastasios V.、Nambu Atsushi、Kubo Yoshihiro、Kawaguchi Yasuo、Kaibuchi Kozo
- Journal Title
  
  Cell Reports
  
  Volume: 40 Issue: 10 Pages: 111309-111309
- DOI
  10.1016/j.celrep.2022.111309
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-20K07965, KAKENHI-PROJECT-21K06427, KAKENHI-PROJECT-21K06428, KAKENHI-PROJECT-21K07257, KAKENHI-PROJECT-22K06438, KAKENHI-PROJECT-19KK0193, KAKENHI-PROJECT-20K07082, KAKENHI-PROJECT-20K07284, KAKENHI-PUBLICLY-21H00196, KAKENHI-PUBLICLY-22H04758, KAKENHI-PUBLICLY-22H04790, KAKENHI-PROJECT-20H03359
[Journal Article] Closed-state inactivation of cardiac, skeletal, and neuronal sodium channels is isoform specific2022
- Author(s)
  Brake N, Mancino AS, Yan Y, Shimomura T, Kubo Y, Khadra A, Bowie D
- Journal Title
  
  J Gen Physiol
  
  Volume: 154(7) Issue: 7
- DOI
  10.1085/jgp.202112921
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-20H03424, KAKENHI-PROJECT-20K07284, KAKENHI-PUBLICLY-22H04758
[Journal Article] Phosphoinositide regulates dynamic movement of the S4 voltage sensor in the second repeat in two-pore channel 32021
- Author(s)
  Hirazawa Kiichi、Tateyama Michihiro、Kubo Yoshihiro、Shimomura Takushi
- Journal Title
  
  Journal of Biological Chemistry
  
  Volume: 297 Issue: 6 Pages: 101425-101425
- DOI
  10.1016/j.jbc.2021.101425
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PUBLICLY-20H05449, KAKENHI-PROJECT-20H03424, KAKENHI-PROJECT-20K07284, KAKENHI-PROJECT-21K06793
[Journal Article] Mechanism of hERG inhibition by gating-modifier toxin, APETx1, deduced by functional characterization2021
- Author(s)
  Matsumura Kazuki、Shimomura Takushi、Kubo Yoshihiro、Oka Takayuki、Kobayashi Naohiro、Imai Shunsuke、Yanase Naomi、Akimoto Madoka、Fukuda Masahiro、Yokogawa Mariko、Ikeda Kazuyoshi、Kurita Jun-ichi、Nishimura Yoshifumi、Shimada Ichio、Osawa Masanori
- Journal Title
  
  BMC Molecular and Cell Biology
  
  Volume: 22 Issue: 1 Pages: 1-16
- DOI
  10.1186/s12860-020-00337-3
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K07284, KAKENHI-PUBLICLY-19H04973, KAKENHI-PUBLICLY-20H05449, KAKENHI-PROJECT-20H03424, KAKENHI-PROJECT-18K06152, KAKENHI-PROJECT-18K06606, KAKENHI-PROJECT-17H06097
[Journal Article] サソリ毒の持つ精巧なNa<sup>+</sup>チャネル調節メカニズム2019
- Author(s)
  下村拓史
- Journal Title
  
  Farumashia
  
  Volume: 55 Issue: 9 Pages: 888-888
- DOI
  10.14894/faruawpsj.55.9_888
- NAID
  130007700060
- ISSN
  0014-8601, 2189-7026
- Language
  Japanese
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-18K15019
[Journal Article] Phosphoinositides modulate the voltage dependence of two-pore channel 32019
- Author(s)
  Shimomura T, Kubo Y
- Journal Title
  
  J Gen Physiol
  
  Volume: 151 Issue: 8 Pages: 986-1006
- DOI
  10.1085/jgp.201812285
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-17H04021, KAKENHI-PROJECT-18K15019
[Presentation] Voltage clamp fluorometry of two-pore channels reveals a unique movement of 2nd S4 helix2024
- Author(s)
  Takushi Shimomura
- Organizer
  IINS Bordeaux - NIPS joint symposium
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K06352
[Presentation] A switching mechanism between PIP2- and voltage-dependence in two-pore channels2024
- Author(s)
  下村　拓史
- Organizer
  第101回　日本生理学会
- Invited
- Data Source
  KAKENHI-PROJECT-23K06352
[Presentation] A switching mechanism between PIP2- and voltage-dependence in two-pore channels2024
- Author(s)
  下村　拓史
- Organizer
  第101回　日本生理学会
- Invited
- Data Source
  KAKENHI-PUBLICLY-22H04758
[Presentation] Voltage clamp fluorometry of two-pore channels reveals a unique movement of 2nd S4 helix2024
- Author(s)
  Takushi Shimomura
- Organizer
  IINS Bordeaux - NIPS joint symposium
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PUBLICLY-22H04758
[Presentation] 遷移金属イオン蛍光共鳴エネルギー移動を利用した電位依存性カチオンチャネルの動的構造解析2023
- Author(s)
  平澤輝一、下村拓史、久保義弘
- Organizer
  第100回日本生理学会大会
- Data Source
  KAKENHI-PUBLICLY-22H04758
[Presentation] Two-pore channelにおける2番目のS4ヘリックスの状態に依存したPIP2および電位依存性ゲーティングの切り替え機構2023
- Author(s)
  下村拓史、平澤輝一、久保義弘
- Organizer
  第100回日本生理学会大会
- Data Source
  KAKENHI-PROJECT-20K07284
[Presentation] Contribution of the 2nd S4 helix for switching PIP2- and voltage-gating modes in two-pore channels2023
- Author(s)
  Takushi Shimomura
- Organizer
  ION CHANNEL MODULATION SYMPOSIUM 2023
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PUBLICLY-22H04758
[Presentation] Two-pore channelにおける2番目のS4ヘリックスの状態に依存したPIP2および電位依存性ゲーティングの切り替え機構2023
- Author(s)
  下村拓史、平澤輝一、久保義弘
- Organizer
  第100回日本生理学会大会
- Data Source
  KAKENHI-PUBLICLY-22H04758
[Presentation] An intermediate state in the 2nd voltage sensor domain is related to the PIP2-gating mode in two-pore channels2023
- Author(s)
  Takushi Shimomura
- Organizer
  The 10th Federation of the Asian and Oceanian Physiological Societies Congress
- Int'l Joint Research
- Data Source
  KAKENHI-PUBLICLY-22H04758
[Presentation] Contribution of the 2nd S4 helix for switching PIP2- and voltage-gating modes in two-pore channels2023
- Author(s)
  Takushi Shimomura
- Organizer
  ION CHANNEL MODULATION SYMPOSIUM 2023
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K06352
[Presentation] 遷移金属イオン蛍光共鳴エネルギー移動を利用した電位依存性カチオンチャネルの動的構造解析2023
- Author(s)
  平澤輝一、下村拓史、久保義弘
- Organizer
  第100回日本生理学会大会
- Data Source
  KAKENHI-PROJECT-20K07284
[Presentation] An intermediate state in the 2nd voltage sensor domain is related to the PIP2-gating mode in two-pore channels2023
- Author(s)
  Takushi Shimomura
- Organizer
  The 10th Federation of the Asian and Oceanian Physiological Societies Congress
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-23K06352
[Presentation] Analysis of the relationship between channel opening and position of the second S4 helix in two-pore Na+ channel 32022
- Author(s)
  下村拓史、平澤輝一、久保義弘
- Organizer
  第99回日本生理学会大会
- Data Source
  KAKENHI-PROJECT-20K07284
[Presentation] 膜電位下蛍光電流同時測定法により明らかになった、Two-pore Channelにおけるリガンド依存性と電位依存性ゲーティングの切り替え・統合の分子基盤2022
- Author(s)
  下村拓史
- Organizer
  第95回日本生化学会大会
- Invited
- Data Source
  KAKENHI-PUBLICLY-22H04758
[Presentation] Analysis of the relationship between channel opening and position of the second S4 helix in two-pore Na+ channel 32022
- Author(s)
  下村拓史、平澤輝一、久保義弘
- Organizer
  第99回日本生理学会大会
- Data Source
  KAKENHI-PUBLICLY-20H05449
[Presentation] 膜電位下蛍光電流同時測定法により明らかになった、Two-pore Channelにおけるリガンド依存性と電位依存性ゲーティングの切り替え・統合の分子基盤2022
- Author(s)
  下村拓史
- Organizer
  第95回日本生化学会大会
- Invited
- Data Source
  KAKENHI-PROJECT-20K07284
[Presentation] Cysteine scanning analysis of the 2nd S4 in two-pore Na+ channel 32021
- Author(s)
  下村拓史、平澤喜一、久保義弘
- Organizer
  第126回日本解剖学会総会・全国学術集会・第98回日本生理学会大会　合同大会
- Data Source
  KAKENHI-PROJECT-20K07284
[Presentation] Cysteine scanning analysis of the 2nd S4 in two-pore Na+ channel 32021
- Author(s)
  下村拓史、平澤喜一、久保義弘
- Organizer
  第126回日本解剖学会総会・全国学術集会・第98回日本生理学会大会　合同大会
- Data Source
  KAKENHI-PUBLICLY-20H05449
[Presentation] PI(3,4)P2-dependent Modulation of the Voltage Dependence in Two-pore Channel 32020
- Author(s)
  Shimomura T、Kubo Y
- Organizer
  64th Annual Meeting of the Biophysical Society
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K15019
[Presentation] PI(3,4)P2- and voltage-dependent gating of two-pore Na+ channel 32020
- Author(s)
  Shimomura T, Hirazawa K, Kubo Y.
- Organizer
  第97回日本生理学会大会（誌上開催）
- Data Source
  KAKENHI-PROJECT-18K15019
[Presentation] Practical Approaches to Protein Structural Information2019
- Author(s)
  下村拓史
- Organizer
  9th FAOPS congress
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K15019
[Presentation] A key interaction for modulation of voltage dependence by phosphoinositides in Two-pore channel 32019
- Author(s)
  下村拓史、久保義弘
- Organizer
  9th FAOPS congress
- Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K15019
[Presentation] Two-Pore Na+ channel 3にみられる長期脱分極刺激依存的な活性亢進の分子メカニズムについての解析2018
- Author(s)
  下村拓史、久保義弘
- Organizer
  第65回中部生理学会
- Data Source
  KAKENHI-PROJECT-18K15019
[Presentation] PIP2-dependent modulation of voltage dependence in Two-Pore Na+ Channel 32018
- Author(s)
  下村拓史、久保義弘
- Organizer
  第8回生理研-霊長研-脳研合同シンポジウム
- Data Source
  KAKENHI-PROJECT-18K15019
[Presentation] Phosphoinositides modulate the voltage dependence in Two-Pore Na+ Channel 3 (TPC3)2018
- Author(s)
  下村拓史、久保義弘
- Organizer
  The 49th NIPS International Symposium
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-18K15019
[Presentation] Two-pore Na+ channel 3の特徴的な電位依存性制御メカニズムについての解析2018
- Author(s)
  下村　拓史、久保　義弘
- Organizer
  第7回新潟脳研－霊長研－生理研合同シンポジウム
- Data Source
  KAKENHI-PROJECT-16K19000
[Presentation] Modulation of the voltage dependence by phosphoinositides in Two-Pore Na+ Channel 3 (TPC3)2018
- Author(s)
  下村　拓史、久保　義弘
- Organizer
  第95回日本生理学会大会
- Data Source
  KAKENHI-PROJECT-16K19000
[Presentation] Two-pore Na+ channel 3（TPC3）の2番目の膜電位センサードメインの特徴的な機能・構造についての解析2017
- Author(s)
  下村　拓史
- Organizer
  第94回日本生理学会大会
- Place of Presentation
  浜松アクトシティコングレスセンターほか　静岡県浜松市
- Year and Date
  2017-03-28
- Data Source
  KAKENHI-PROJECT-16K19000
[Presentation] Two pore Na+ channel 3（TPC3）の電位依存的活性化メカニズム2017
- Author(s)
  下村　拓史
- Organizer
  生理学研究所プロジェクト「細胞・システム作動機構の理解に向けた、生体タンパク質分子の構造と機能のダイナミクス研究の拠点形成」平成28年度末シンポジウム
- Place of Presentation
  生理学研究所　愛知県岡崎市
- Year and Date
  2017-03-13
- Data Source
  KAKENHI-PROJECT-16K19000
[Presentation] Two-pore Na+ channel 3 (TPC3)の2つのS4へリックス上の正電荷は、電位依存性に異なる貢献をする2016
- Author(s)
  下村　拓史
- Organizer
  第63回中部日本生理学会
- Place of Presentation
  自然科学研究機構岡崎コンファレンスセンター　愛知県岡崎市
- Year and Date
  2016-11-04
- Data Source
  KAKENHI-PROJECT-16K19000

1. 久保義弘

# of Collaborated Projects: 0 results

# of Collaborated Products: 3 results
2. 黒田啓介

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Shimomura Takushi 下村 拓史

Research Projects

Research Products

Co-Researchers

光生理学的手法を用いたTwo pore channelにおけるイオン選択性制御の分子基盤の解明Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

非天然アミノ酸を用いた汎用かつ簡便なタンパク質光感受化法Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Unnatural amino acid-based state-specific analysis of ion channelsPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

光異性化アミノ酸導入による、リガンド依存性イオンチャネルの光制御化法の開発Principal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Analysis of the involvment of TPC3 channel for polyspermyPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Analysis of the unique voltage-dependent mechanism of a Na+ channel by voltage-clamp fluorometryPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Journal Article] Conformational rearrangements in the second voltage sensor domain switch PIP2- and voltage-gating modes in two-pore channels2023

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Dopamine drives neuronal excitability via KCNQ channel phosphorylation for reward behavior2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Closed-state inactivation of cardiac, skeletal, and neuronal sodium channels is isoform specific2022

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Phosphoinositide regulates dynamic movement of the S4 voltage sensor in the second repeat in two-pore channel 32021

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Mechanism of hERG inhibition by gating-modifier toxin, APETx1, deduced by functional characterization2021

Author(s)

Journal Title

DOI

Data Source

[Journal Article] サソリ毒の持つ精巧なNa<sup>+</sup>チャネル調節メカニズム2019

Author(s)

Journal Title

DOI

NAID

ISSN

Language

Data Source

[Journal Article] Phosphoinositides modulate the voltage dependence of two-pore channel 32019

Author(s)

Journal Title

DOI

Data Source

[Presentation] Voltage clamp fluorometry of two-pore channels reveals a unique movement of 2nd S4 helix2024

Author(s)

Shimomura Takushi 下村拓史