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LIM KEE SIANG  LIM KEE・SIANG

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Lim Kee Siang  LIM KEE・SIANG

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Researcher Number 60842987
Other IDs
Affiliation (Current) 2025: 金沢大学, ナノ生命科学研究所, 特任助教
Affiliation (based on the past Project Information) *help 2019 – 2024: 金沢大学, ナノ生命科学研究所, 特任助教
Review Section/Research Field
Principal Investigator
Basic Section 49060:Virology-related / 0803:Pathology, infection/immunology, and related fields
Except Principal Investigator
Medium-sized Section 37:Biomolecular chemistry and related fields
Keywords
Principal Investigator
High-speed AFM / viral fusion protein / Influenza A / Hemagglutinin / HS-AFM / Nuclear transport / Nuclear pore complex / RNA viruses / Nucleocapsid protein / Exosome … More / High Speed AFM / Virulence factor / Influenz A / Fusogenic transition / Virulence factors / Direct visualization / exosome / fusogenic transition / Viral fusion protein / Viral Fusion Protein / COVID-19 / Neutralizing antibodies / SARS-CoV-2 spike protein / HS-AFM nanoimaging / SARS-CoV-2 / Spike protein / Viral fusion proteins … More
Except Principal Investigator
相分離 / 翻訳後修飾 / 液-液相分離 / 核膜孔 / 核膜孔複合体 Less
  • Research Projects

    (4 results)
  • Research Products

    (36 results)
  • Co-Researchers

    (5 People)
  •  Nanoscopic elucidation of dynamic behavior of RNA viral nucleocapsid proteins using high-speed atomic force microscopy (HS-AFM)Principal Investigator

    • Principal Investigator
      LIM KEE・SIANG
    • Project Period (FY)
      2024 – 2027
    • Research Category
      Grant-in-Aid for Early-Career Scientists
    • Review Section
      Basic Section 49060:Virology-related
    • Research Institution
      Kanazawa University
  •  Functional Dynamics of Liquid-Liquid Phase Separation Mediated by Nuclear Pore Complex

    • Principal Investigator
      Wong Richard
    • Project Period (FY)
      2021 – 2022
    • Research Category
      Grant-in-Aid for Challenging Research (Exploratory)
    • Review Section
      Medium-sized Section 37:Biomolecular chemistry and related fields
    • Research Institution
      Kanazawa University
  •  Real-time observation of native conformations and molecular behaviors of viral fusion proteins using high-speed atomic force microscopy (HS-AFM)Principal Investigator

    • Principal Investigator
      Lim Kee Siang
    • Project Period (FY)
      2020 – 2023
    • Research Category
      Grant-in-Aid for Early-Career Scientists
    • Review Section
      Basic Section 49060:Virology-related
    • Research Institution
      Kanazawa University
  •  Direct visualization of conformational dynamics of hemagglutinin and interaction between hemagglutinin and exosomes using high-speed atomic force microscopy.Principal Investigator

    • Principal Investigator
      Lim Kee Siang
    • Project Period (FY)
      2019 – 2020
    • Research Category
      Grant-in-Aid for Research Activity Start-up
    • Review Section
      0803:Pathology, infection/immunology, and related fields
    • Research Institution
      Kanazawa University

All 2024 2023 2022 2021 2020 2019

All Journal Article Presentation

  • [Journal Article] Crafty mimicry grants nuclear pore entry to HIV2024

    • Author(s)
      Lim Keesiang、Hazawa Masaharu、Wong Richard W.
    • Journal Title

      Cell Host & Microbe

      Volume: 32 Issue: 4 Pages: 441-442

    • DOI

      10.1016/j.chom.2024.03.004

    • Peer Reviewed / Open Access / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-20K16262, KAKENHI-PUBLICLY-22H05537, KAKENHI-PUBLICLY-23H04278, KAKENHI-PROJECT-23K23476
  • [Journal Article] Nanoscopic Elucidation of Spontaneous Self-Assembly of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Open Reading Frame 6 (ORF6) Protein2023

    • Author(s)
      Nishide Goro、Lim Keesiang、Tamura Maiki、Kobayashi Akiko、Zhao Qingci、Hazawa Masaharu、Ando Toshio、Nishida Noritaka、Wong Richard W.
    • Journal Title

      The Journal of Physical Chemistry Letters

      Volume: 14 Issue: 38 Pages: 8385-8396

    • DOI

      10.1021/acs.jpclett.3c01440

    • Peer Reviewed / Open Access / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-22K06924, KAKENHI-PROJECT-20K16262, KAKENHI-PLANNED-21H05250, KAKENHI-PUBLICLY-22H05537, KAKENHI-PUBLICLY-23H04278, KAKENHI-PROJECT-22H00405, KAKENHI-PROJECT-23K23476
  • [Journal Article] Nanoscopic Assessment of Anti-SARS-CoV-2 Spike Neutralizing Antibody Using High-Speed AFM.2023

    • Author(s)
      Keesiang Lim, Goro Nishide, Elma Sakinatus Sajidah, Tomoyoshi Yamano, Yujia Qiu, Takeshi Yoshida, Akiko Kobayashi, Masaharu Hazawa, Toshio Ando, Rikinari Hanayama, Richard W Wong
    • Journal Title

      Nano letters

      Volume: 23 Issue: 2 Pages: 619-628

    • DOI

      10.1021/acs.nanolett.2c04270

    • Peer Reviewed / Open Access / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-21K07065, KAKENHI-PROJECT-21K07173, KAKENHI-PROJECT-22K06924, KAKENHI-PUBLICLY-21H05744, KAKENHI-PROJECT-19H03433, KAKENHI-PROJECT-21K19043, KAKENHI-PROJECT-20K16262, KAKENHI-PUBLICLY-22H05537, KAKENHI-PUBLICLY-23H04278, KAKENHI-PROJECT-22H00405, KAKENHI-PROJECT-23K23476
  • [Journal Article] Recent advances in sensing the inter-biomolecular interactions at the nanoscale ? A comprehensive review of AFM-based force spectroscopy2023

    • Author(s)
      Lostao Anabel、Lim KeeSiang、Pallar?s Mar?a Carmen、Ptak Arkadiusz、Marcuello Carlos
    • Journal Title

      International Journal of Biological Macromolecules

      Volume: 238 Pages: 124089-124089

    • DOI

      10.1016/j.ijbiomac.2023.124089

    • Peer Reviewed / Open Access / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-20K16262
  • [Journal Article] NSP9 of SARS-CoV-2 attenuates nuclear transport by hampering nucleoporin 62 dynamics and functions in host cells2022

    • Author(s)
      Makiyama Kei、Hazawa Masaharu、Kobayashi Akiko、Lim Keesiang、Voon Dominic C.、Wong Richard W.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 586 Pages: 137-142

    • DOI

      10.1016/j.bbrc.2021.11.046

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-19K07398, KAKENHI-PROJECT-20K16262, KAKENHI-PUBLICLY-21H05744, KAKENHI-PUBLICLY-22H05537, KAKENHI-PROJECT-21K19043, KAKENHI-PROJECT-23K23476
  • [Journal Article] Spatiotemporal tracking of small extracellular vesicle nanotopology in response to physicochemical stresses revealed by HS-AFM.2022

    • Author(s)
      Elma Sakinatus Sajidah, Keesiang Lim, Tomoyoshi Yamano, Goro Nishide, Yujia Qiu, Takeshi Yoshida, Hanbo Wang, Akiko Kobayashi, Masaharu Hazawa, Firli R P Dewi, Rikinari Hanayama, Toshio Ando, Richard W Wong
    • Journal Title

      Journal of extracellular vesicles

      Volume: 11 Issue: 11 Pages: 12275-12275

    • DOI

      10.1002/jev2.12275

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-21K07065, KAKENHI-PROJECT-21K07173, KAKENHI-PROJECT-22K06924, KAKENHI-PROJECT-20K16262, KAKENHI-PUBLICLY-21H05744, KAKENHI-PUBLICLY-22H05537, KAKENHI-PROJECT-22H00405, KAKENHI-PROJECT-19H03433, KAKENHI-PROJECT-21K19043, KAKENHI-PROJECT-23K23476
  • [Journal Article] Discovery of a Novel Aminocyclopropenone Compound That Inhibits BRD4-Driven Nucleoporin NUP210 Expression and Attenuates Colorectal Cancer Growth2022

    • Author(s)
      Kondo Hiroya、Mishiro Kenji、Iwashima Yuki、Qiu Yujia、Kobayashi Akiko、Lim Keesiang、Domoto Takahiro、Minamoto Toshinari、Ogawa Kazuma、Kunishima Munetaka、Hazawa Masaharu、Wong Richard W.
    • Journal Title

      Cells

      Volume: 11 Issue: 3 Pages: 317-317

    • DOI

      10.3390/cells11030317

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-19K07398, KAKENHI-PROJECT-19K07710, KAKENHI-PROJECT-20K15953, KAKENHI-PROJECT-19H03727, KAKENHI-PROJECT-20K16262, KAKENHI-PUBLICLY-21H05744, KAKENHI-PUBLICLY-22H05537, KAKENHI-PROJECT-21K19043, KAKENHI-PROJECT-23K24403, KAKENHI-PROJECT-23K23476
  • [Journal Article] High-Speed Atomic Force Microscopy Reveals Spatiotemporal Dynamics of Histone Protein H2A Involution by DNA Inchworming2021

    • Author(s)
      Nishide Goro、Lim Keesiang、Mohamed Mahmoud Shaaban、Kobayashi Akiko、Hazawa Masaharu、Watanabe-Nakayama Takahiro、Kodera Noriyuki、Ando Toshio、Wong Richard W.
    • Journal Title

      The Journal of Physical Chemistry Letters

      Volume: 12 Issue: 15 Pages: 3837-3846

    • DOI

      10.1021/acs.jpclett.1c00697

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-17H06121, KAKENHI-PROJECT-19K07398, KAKENHI-PUBLICLY-21H05744, KAKENHI-PROJECT-20H00327, KAKENHI-PROJECT-21K19043, KAKENHI-PROJECT-20K16262
  • [Journal Article] How SARS-CoV-2 and Other Viruses Build an Invasion Route to Hijack the Host Nucleocytoplasmic Trafficking System2021

    • Author(s)
      Sajidah Elma Sakinatus、Lim Keesiang、Wong Richard W.
    • Journal Title

      Cells

      Volume: 10 Issue: 6 Pages: 1424-1424

    • DOI

      10.3390/cells10061424

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PUBLICLY-21H05744, KAKENHI-PROJECT-21K19043, KAKENHI-PROJECT-20K16262
  • [Journal Article] Millisecond dynamic of SARS‐CoV‐2 spike and its interaction with ACE2 receptor and small extracellular vesicles2021

    • Author(s)
      Lim Keesiang、Nishide Goro、Yoshida Takeshi、Watanabe‐Nakayama Takahiro、Kobayashi Akiko、Hazawa Masaharu、Hanayama Rikinari、Ando Toshio、Wong Richard W.
    • Journal Title

      Journal of Extracellular Vesicles

      Volume: 10 Issue: 14

    • DOI

      10.1002/jev2.12170

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-19K07398, KAKENHI-PUBLICLY-21H05744, KAKENHI-PROJECT-17H06121, KAKENHI-PROJECT-19H03433, KAKENHI-PROJECT-21K07173, KAKENHI-PROJECT-21K19043, KAKENHI-PROJECT-20K16262
  • [Journal Article] High-Speed AFM Reveals Molecular Dynamics of Human Influenza A Hemagglutinin and Its Interaction with Exosomes2020

    • Author(s)
      Lim Keesiang、Kodera Noriyuki、Wang Hanbo、Mohamed Mahmoud Shaaban、Hazawa Masaharu、Kobayashi Akiko、Yoshida Takeshi、Hanayama Rikinari、Yano Seiji、Ando Toshio、Wong Richard W.
    • Journal Title

      Nano Letters

      Volume: 20 Issue: 9 Pages: 6320-6328

    • DOI

      10.1021/acs.nanolett.0c01755

    • Peer Reviewed / Open Access / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-19K07398, KAKENHI-PROJECT-20K07568, KAKENHI-PROJECT-19K22578, KAKENHI-PROJECT-20K16262, KAKENHI-PROJECT-17H06121, KAKENHI-PROJECT-20H00327, KAKENHI-PROJECT-19H03433, KAKENHI-PROJECT-19K23841
  • [Presentation] Nanoscopic Evaluation of Anti-SARS-CoV-2 Spike Neutralizing Antibody Using High-Speed AFM2023

    • Author(s)
      Keesiang Lim
    • Organizer
      the 7th WPI NanoLSI Symposium
    • Data Source
      KAKENHI-PROJECT-20K16262
  • [Presentation] Nanoscopic Evaluation of Anti-SARS-CoV-2 Spike Neutralizing Antibody Using High-Speed AFM.2023

    • Author(s)
      Keesiang Lim
    • Organizer
      The 70th Annual Meeting of the Japanese Society for Virology
    • Data Source
      KAKENHI-PROJECT-20K16262
  • [Presentation] Nanoscopic Evaluation of Anti-SARS-CoV-2 Spike Neutralizing Antibody Using High-Speed AFM2023

    • Author(s)
      Keesiang Lim
    • Organizer
      The 23rd annual meeting of the Protein Science Society of Japan
    • Data Source
      KAKENHI-PROJECT-20K16262
  • [Presentation] Spatiotemporal tracking of SARS-CoV-2 spike (S) protein and its interaction with ACE2 receptor and small extracellular vesicles2022

    • Author(s)
      Keesiang Lim
    • Organizer
      he 60th Annual Meeting of the Japanese Society for Biophysics
    • Data Source
      KAKENHI-PROJECT-20K16262
  • [Presentation] Millisecond tracking of structural dynamics of SARS-CoV-2 spike protein and its interaction with ACE2 receptor and small extracellular vesicles.2022

    • Author(s)
      Keesiang Lim
    • Organizer
      The 95th Annual Meeting of the Japanese Society for Biochemistry
    • Data Source
      KAKENHI-PROJECT-20K16262
  • [Presentation] Structural dynamics of SARS-CoV-2 spike (S) protein and its interaction with ACE2 receptor and small extracellular vesicle2022

    • Author(s)
      Keesiang Lim
    • Organizer
      The 69th Annual Meeting of the Japanese Society for Virology
    • Data Source
      KAKENHI-PROJECT-20K16262
  • [Presentation] Structural dynamics of SARS-CoV-2 spike (S) protein and its interaction with ACE2 receptor and small extracellular vesicles2022

    • Author(s)
      Keesiang Lim
    • Organizer
      Cross-Scale Biology 1st Wakate meeting
    • Data Source
      KAKENHI-PROJECT-20K16262
  • [Presentation] Conformational dynamics of SARS-CoV-2 spike (S) protein and its interaction with ACE2 receptor and small extracellular vesicles.2022

    • Author(s)
      Keesiang Lim
    • Organizer
      The 74th Annual Meeting of the Japanese Society for Cell Biology
    • Data Source
      KAKENHI-PROJECT-20K16262
  • [Presentation] 高速 AFM を用いた DNA インチワーミングによるヒストン H2A ラッピング動態の実時空間イメージング2021

    • Author(s)
      西出梧朗、Keesiang Lim、Mahmoud Shaaban Mohamed、小林亜紀子、羽澤勝治、中山隆宏、古寺哲幸、安藤敏夫、Richard Wong
    • Organizer
      日本生化学会北陸支部第 39 回大会
    • Data Source
      KAKENHI-PROJECT-21K19043
  • [Presentation] 高速 AFM によるヒストン H2A トポグラフィーとその DNA コンパクション時の時空間ダイナミクスの解明2021

    • Author(s)
      西出梧朗、Keesiang Lim、小林亜紀子、羽澤勝治、中山隆宏、古寺哲幸、安藤敏夫、Richard Wong
    • Organizer
      第21回日本蛋白質科学会年会
    • Data Source
      KAKENHI-PROJECT-21K19043
  • [Presentation] Investigation of real-time dynamic histone H2A-DNA interaction and H2A-DNA condensation/de-condensation using high-speed AFM2021

    • Author(s)
      西出梧朗、Keesiang LIM、小林亜紀子、羽澤勝治、中山隆宏、古寺哲幸、安藤敏夫、Richard WONG
    • Organizer
      第59回日本生物物理学会年会
    • Data Source
      KAKENHI-PROJECT-21K19043
  • [Presentation] 核膜孔複合体による核膜近傍での転写制御機構2021

    • Author(s)
      岩嶋友紀、羽澤勝治、Qiu Yujia、Dini Kurnia Ikliptikawati、西出 梧朗、小林 亜紀子、Lim Kee Siang、Richard Wong
    • Organizer
      第44回日本分子生物学会年会
    • Data Source
      KAKENHI-PROJECT-21K19043
  • [Presentation] 核内相分離の制御を可能とする新規アミノシクロプロペノン化合物の発見2021

    • Author(s)
      近藤 寛弥、三代憲司、岩嶋友紀、Qiu Yujia、稲葉有香、小林亜紀子、Keesiang Lim、源利成、小川数馬、國嶋崇隆、羽澤勝治、Richard Wong
    • Organizer
      第39回染色体ワークショップ・第20回核ダイナミクス研究会
    • Data Source
      KAKENHI-PROJECT-21K19043
  • [Presentation] 高速AFMを用いたヒストンH2A-DNA実時空間イメージングによるヌクレオソーム凝縮プロセスとその環境の解明2021

    • Author(s)
      西出梧朗、Keesiang Lim、Mahmoud Shaaban Mohamed、小林亜紀子、羽澤勝治、中山隆宏、古寺哲幸、安藤敏夫、Richard Wong
    • Organizer
      第94回日本生化学大会
    • Data Source
      KAKENHI-PROJECT-21K19043
  • [Presentation] 核膜孔複合体による転写制御機構2021

    • Author(s)
      岩嶋友紀、羽澤勝治、Qiu Yujia、Dini Kurnia Ikliptikawati1、西 出 梧朗、小林 亜紀子、Lim Kee Siang、Richard Wong
    • Organizer
      第39回染色体ワークショップ・第20回核ダイナミクス研究会
    • Data Source
      KAKENHI-PROJECT-21K19043
  • [Presentation] ヒストンH2A-DNAダイナミクスの実時空間イメージング2021

    • Author(s)
      西出梧朗、Keesiang Lim、小林亜紀子、羽澤勝治、稲葉有香、中山隆宏、古寺哲幸、安藤敏夫、Richard Wong
    • Organizer
      第39回染色体ワークショップ・第20回核ダイナミクス研究会
    • Data Source
      KAKENHI-PROJECT-21K19043
  • [Presentation] 高速 AFM を用いた DNA インチワーミングによるヒストン H2A ラッピング動態の実時空間イメージング2021

    • Author(s)
      西出梧朗、Keesiang Lim、小林亜紀子、羽澤勝治、中山隆宏、古寺哲幸、安藤敏夫、Richard Wong
    • Organizer
      第73回日本細胞生物学会大会
    • Data Source
      KAKENHI-PROJECT-21K19043
  • [Presentation] Molecular Dynamic of Coronavirus Spike Protein2021

    • Author(s)
      Keesiang Lim
    • Organizer
      The Molecular Biology Society of Japan
    • Data Source
      KAKENHI-PROJECT-20K16262
  • [Presentation] Molecular Dynamic of Coronavirus Spike Protein2021

    • Author(s)
      Keesiang Lim, Goro Nishide, Takeshi Yoshida, Takahiro Watanabe-Nakayama, Akiko Kobayashi, Masaharu Hazawa, Rikinari Hanayama, Toshio Ando, Richard W. Wong
    • Organizer
      第44回日本分子生物学会年会
    • Data Source
      KAKENHI-PROJECT-21K19043
  • [Presentation] High-Speed AFM Reveals Molecular Dynamics of Human Influenza A Hemagglutinin and Its Interaction with Exosomes2020

    • Author(s)
      Lim Kee Siang
    • Organizer
      WPI NanoLSI Symposium (4th)
    • Data Source
      KAKENHI-PROJECT-20K16262
  • [Presentation] High-Speed AFM Reveals Molecular Dynamics of Human Influenza A Hemagglutinin and Its Interaction with Exosomes2020

    • Author(s)
      Lim Kee Siang
    • Organizer
      MBSJ (The Molecular Biology Society of Japan) 2020
    • Data Source
      KAKENHI-PROJECT-19K23841
  • [Presentation] Direct visualization of avian influenza H5N1 hemagglutinin precursor and its conformational changes by high-speed atomic force microscopy.2020

    • Author(s)
      Lim Kee Siang
    • Organizer
      1st WPI NanoLSI-iCeMS Joint Symposium on Nanoimaging and Advanced Materials for Life Science
    • Data Source
      KAKENHI-PROJECT-19K23841
  • [Presentation] High-Speed AFM Reveals Molecular Dynamics of Human Influenza A Hemagglutinin and Its Interaction with Exosomes2020

    • Author(s)
      Lim Kee Siang
    • Organizer
      MBSJ (The Molecular Biology Society of Japan) 2020
    • Data Source
      KAKENHI-PROJECT-20K16262
  • [Presentation] High-Speed AFM Reveals Molecular Dynamics of Human Influenza A Hemagglutinin and Its Interaction with Exosomes2020

    • Author(s)
      Lim Kee Siang
    • Organizer
      WPI NanoLSI Symposium (4th)
    • Data Source
      KAKENHI-PROJECT-19K23841
  • [Presentation] Direct visualization of avian influenza H5N1 Hemagglutinin precursor and its conformational change by high-speed atomic force microscopy.2019

    • Author(s)
      Lim Kee Siang
    • Organizer
      MBSJ
    • Data Source
      KAKENHI-PROJECT-19K23841
  • 1.  Wong Richard (30464035)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 11 results
  • 2.  小林 亜紀子 (00345662)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 15 results
  • 3.  羽澤 勝治 (40622460)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 10 results
  • 4.  山野 友義
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 2 results
  • 5.  安藤 敏夫
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 1 results

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