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mizuuchi ryo  水内 良

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Mizuuchi Ryo  水内 良

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Researcher Number 60845535
Affiliation (Current) 2025: 早稲田大学, 理工学術院, 准教授
Affiliation (based on the past Project Information) *help 2025: 早稲田大学, 理工学術院, 准教授
2023 – 2024: 早稲田大学, 理工学術院, 専任講師
2019 – 2022: 東京大学, 大学院総合文化研究科, 特任助教
Review Section/Research Field
Principal Investigator
Basic Section 43040:Biophysics-related / Transformative Research Areas, Section (IV) / 0703:Biology at organismal to population levels and anthropology, and related fields
Keywords
Principal Investigator
RNA / 人工細胞 / 再構成 / 生命の起源 / 進化 / 自己複製 / 液―液相分離 / 分子通信 / 無細胞翻訳系 / ゲノム複製 … More / 多細胞 / 進化分子工学 / 自己触媒 / 前生物化学 / 分子ビーコン / 翻訳システム / 実験進化 / 複雑化 / 生命の初期進化 Less
  • Research Projects

    (5 results)
  • Research Products

    (60 results)
  • Co-Researchers

    (1 People)
  •  Development of minimal, evolvable self-reproducing RNAPrincipal Investigator

    • Principal Investigator
      水内 良
    • Project Period (FY)
      2025 – 2029
    • Research Category
      Grant-in-Aid for Scientific Research (B)
    • Review Section
      Basic Section 43040:Biophysics-related
    • Research Institution
      Waseda University
  •  Spatiotemporal regulation of genome replication in an artificial multicellular systemPrincipal Investigator

    • Principal Investigator
      水内 良
    • Project Period (FY)
      2023 – 2024
    • Research Category
      Grant-in-Aid for Transformative Research Areas (A)
    • Review Section
      Transformative Research Areas, Section (IV)
    • Research Institution
      Waseda University
  •  Construction of a minimal self-reproducing RNAPrincipal Investigator

    • Principal Investigator
      水内 良
    • Project Period (FY)
      2023 – 2024
    • Research Category
      Grant-in-Aid for Early-Career Scientists
    • Review Section
      Basic Section 43040:Biophysics-related
    • Research Institution
      Waseda University
  •  Development of an artificial multicellular genome replication systemPrincipal Investigator

    • Principal Investigator
      水内 良
    • Project Period (FY)
      2021 – 2022
    • Research Category
      Grant-in-Aid for Transformative Research Areas (A)
    • Review Section
      Transformative Research Areas, Section (IV)
    • Research Institution
      The University of Tokyo
  •  Experimental verification of a hypothesis of early evolution of complexity using a primitive life-like systemPrincipal Investigator

    • Principal Investigator
      Mizuuchi Ryo
    • Project Period (FY)
      2019 – 2020
    • Research Category
      Grant-in-Aid for Research Activity Start-up
    • Review Section
      0703:Biology at organismal to population levels and anthropology, and related fields
    • Research Institution
      The University of Tokyo

All 2024 2023 2022 2021 2020 2019

All Journal Article Presentation

  • [Journal Article] Constraint on an RNA world on montmorillonite clay2024

    • Author(s)
      Mizuuchi Ryo
    • Journal Title

      Biophysical Journal

      Volume: 123 Issue: 4 Pages: 421-423

    • DOI

      10.1016/j.bpj.2024.01.020

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-23K14153, KAKENHI-PUBLICLY-23H04403
  • [Journal Article] Emergence of linkage between cooperative RNA replicators encoding replication and metabolic enzymes through experimental evolution2023

    • Author(s)
      Ueda Kensuke、Mizuuchi Ryo、Ichihashi Norikazu
    • Journal Title

      PLOS Genetics

      Volume: 19 Issue: 8 Pages: e1010471-e1010471

    • DOI

      10.1371/journal.pgen.1010471

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-23K14153, KAKENHI-PROJECT-22K21344, KAKENHI-PUBLICLY-22H05402, KAKENHI-PUBLICLY-23H04403
  • [Journal Article] How prebiotic complexity increases through Darwinian evolution2023

    • Author(s)
      Yukawa Kohtoh、Mizuuchi Ryo、Ichihashi Norikazu
    • Journal Title

      Current Opinion in Systems Biology

      Volume: 34 Pages: 100456-100456

    • DOI

      10.1016/j.coisb.2023.100456

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-23K14153, KAKENHI-PROJECT-22K21344, KAKENHI-PUBLICLY-23H04403
  • [Journal Article] Minimal RNA self-reproduction discovered from a random pool of oligomers2023

    • Author(s)
      Mizuuchi Ryo、Ichihashi Norikazu
    • Journal Title

      Chemical Science

      Volume: 14 Issue: 28 Pages: 7656-7664

    • DOI

      10.1039/d3sc01940c

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-23K14153, KAKENHI-PROJECT-22K21344, KAKENHI-PUBLICLY-22H05402, KAKENHI-PUBLICLY-23H04403
  • [Journal Article] Cell-free expression of RuBisCO for ATP production in the synthetic cells2023

    • Author(s)
      Sugii Shugo、Hagino Katsumi、Mizuuchi Ryo、Ichihashi Norikazu
    • Journal Title

      Synthetic Biology

      Volume: 8 Issue: 1

    • DOI

      10.1093/synbio/ysad016

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-23K14153, KAKENHI-PROJECT-23KJ0558, KAKENHI-PROJECT-22K21344, KAKENHI-PUBLICLY-22H05402, KAKENHI-PUBLICLY-23H04403
  • [Journal Article] Evolutionary transition from a single RNA replicator to a multiple replicator network2022

    • Author(s)
      Mizuuchi, R., Furubayashi, T., Ichihashi, N
    • Journal Title

      Nature Communications

      Volume: 13 Issue: 1 Pages: 1460-1460

    • DOI

      10.1038/s41467-022-29113-x

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PUBLICLY-20H04859, KAKENHI-PUBLICLY-21H05867, KAKENHI-PROJECT-22K14794, KAKENHI-PROJECT-22KJ0540, KAKENHI-PUBLICLY-22H05402
  • [Journal Article] Plausible pathway for a host-parasite molecular replication network to increase its complexity through Darwinian evolution2022

    • Author(s)
      Kamiura Rikuto、Mizuuchi Ryo、Ichihashi Norikazu
    • Journal Title

      PLOS Computational Biology

      Volume: 18 Issue: 12 Pages: e1010709-e1010709

    • DOI

      10.1371/journal.pcbi.1010709

    • Peer Reviewed / Open Access / Int'l Joint Research
    • Data Source
      KAKENHI-PUBLICLY-21H05867, KAKENHI-PROJECT-20J21802
  • [Journal Article] Experimental evidence for the correlation between RNA structural fluctuations and the frequency of beneficial mutations2022

    • Author(s)
      Maeda Yutaro、Mizuuchi Ryo、Shigenobu Shuji、Shibai Atsushi、Kotani Hazuki、Furusawa Chikara、Ichihashi Norikazu
    • Journal Title

      RNA

      Volume: 28 Pages: 1659-1667

    • DOI

      10.1261/rna.079291.122

    • Data Source
      KAKENHI-PUBLICLY-21H05867
  • [Journal Article] Relaxed Substrate Specificity in Qβ Replicase through Long-Term In Vitro Evolution2021

    • Author(s)
      Yukawa, K., Mizuuchi, R., Ichihashi, N
    • Journal Title

      Life

      Volume: 12 Issue: 1 Pages: 32-32

    • DOI

      10.3390/life12010032

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PUBLICLY-20H04859, KAKENHI-PUBLICLY-21H05867, KAKENHI-PUBLICLY-22H05402
  • [Journal Article] Primitive Compartmentalization for the Sustainable Replication of Genetic Molecules2021

    • Author(s)
      Mizuuchi Ryo、Ichihashi Norikazu
    • Journal Title

      Life

      Volume: 11 Issue: 3 Pages: 1-17

    • DOI

      10.3390/life11030191

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-19K23763
  • [Journal Article] Development and evolution of a primitive life-like molecular replication system2021

    • Author(s)
      水内 良
    • Journal Title

      Viva Origino

      Volume: 49 Issue: 3 Pages: 10

    • DOI

      10.50968/vivaorigino.49_10

    • NAID

      130008130254

    • ISSN
      0910-4003, 1346-6933
    • Language
      Japanese
    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PUBLICLY-21H05867
  • [Journal Article] Emergence and diversification of a host-parasite RNA ecosystem through Darwinian evolution2020

    • Author(s)
      Furubayashi Taro、Ueda Kensuke、Bansho Yohsuke、Motooka Daisuke、Nakamura Shota、Mizuuchi Ryo、Ichihashi Norikazu
    • Journal Title

      eLife

      Volume: 9 Pages: 1-15

    • DOI

      10.7554/elife.56038

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-19K23763, KAKENHI-PUBLICLY-20H04859
  • [Journal Article] Translation-coupled RNA replication and parasitic replicators in membrane-free compartments2020

    • Author(s)
      Mizuuchi Ryo、Ichihashi Norikazu
    • Journal Title

      Chemical Communications

      Volume: 56 Issue: 87 Pages: 13453-13456

    • DOI

      10.1039/d0cc06606k

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-19K23763
  • [Journal Article] In vitro evolution of phi29 DNA polymerases through compartmentalized gene expression and rolling-circle replication2020

    • Author(s)
      Sakatani Yoshihiro、Mizuuchi Ryo、Ichihashi Norikazu
    • Journal Title

      Protein Engineering, Design and Selection

      Volume: 32 Issue: 11 Pages: 481-487

    • DOI

      10.1093/protein/gzaa011

    • Data Source
      KAKENHI-PROJECT-19K23763
  • [Journal Article] Structural transition of replicable RNAs during in vitro evolution with Qβ replicase2019

    • Author(s)
      Mizuuchi Ryo、Usui Kimihito、Ichihashi Norikazu
    • Journal Title

      RNA

      Volume: 26 Issue: 1 Pages: 83-90

    • DOI

      10.1261/rna.073106.119

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-19K23763, KAKENHI-PUBLICLY-18H04820, KAKENHI-PROJECT-15KT0080
  • [Presentation] 生命の起源を追体験する2024

    • Author(s)
      水内良
    • Organizer
      定量生物学の会 第十一回年会
    • Invited
    • Data Source
      KAKENHI-PUBLICLY-23H04403
  • [Presentation] 原始的なRNA 集団と自己複製体の探究2024

    • Author(s)
      水内良、柿崎二郎、佐藤颯、市橋伯一
    • Organizer
      第48回 生命の起原および進化学会学術講演会
    • Data Source
      KAKENHI-PUBLICLY-23H04403
  • [Presentation] 生命の起源を追体験する2024

    • Author(s)
      水内良
    • Organizer
      定量生物学の会 第十一回年会
    • Invited
    • Data Source
      KAKENHI-PROJECT-23K14153
  • [Presentation] 原始的なRNA 集団と自己複製体の探究2024

    • Author(s)
      水内良、柿崎二郎、佐藤颯、市橋伯一
    • Organizer
      第48回 生命の起原および進化学会学術講演会
    • Data Source
      KAKENHI-PROJECT-23K14153
  • [Presentation] 原始生命モデルの実験進化で観測不能な生命の起源に迫る2023

    • Author(s)
      水内良
    • Organizer
      日本進化学会第25回大会
    • Invited
    • Data Source
      KAKENHI-PROJECT-23K14153
  • [Presentation] Minimal RNA self-reproduction discovered from a random pool of oligomers2023

    • Author(s)
      Ryo Mizuuchi, Norikazu Ichihashi
    • Organizer
      Molecular Origins of Life
    • Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-23K14153
  • [Presentation] Emergence and evolution of host-parasite ecosystems in a molecular replication system2023

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      Confect Symposium 2023 “Marine Microbial Virus Ecology and Evolution”
    • Invited / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-23K14153
  • [Presentation] 原始的なRNA集団の調査から見つかった自己複製する最小のRNA2023

    • Author(s)
      水内良、市橋伯一
    • Organizer
      「細胞を創る」研究会 16.0
    • Data Source
      KAKENHI-PROJECT-23K14153
  • [Presentation] RNA replication systems and evolution: for understanding the origins of life2023

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      SPEED × Bottom-up Biotech × ELSI joint workshop
    • Invited / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-23K14153
  • [Presentation] Minimal RNA self-reproduction discovered from a random pool of oligomers2023

    • Author(s)
      Ryo Mizuuchi, Norikazu Ichihashi
    • Organizer
      Molecular Origins of Life
    • Int'l Joint Research
    • Data Source
      KAKENHI-PUBLICLY-23H04403
  • [Presentation] Emergence and evolution of host-parasite ecosystems in a molecular replication system2023

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      Confect Symposium 2023 “Marine Microbial Virus Ecology and Evolution
    • Invited / Int'l Joint Research
    • Data Source
      KAKENHI-PUBLICLY-23H04403
  • [Presentation] 原始生命モデルの実験進化で観測不能な生命の起源に迫る2023

    • Author(s)
      水内良
    • Organizer
      日本進化学会第25回大会
    • Invited
    • Data Source
      KAKENHI-PUBLICLY-23H04403
  • [Presentation] Minimal RNA self-reproduction discovered from a random pool of oligomers2023

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      第61回日本生物物理学会年会
    • Invited
    • Data Source
      KAKENHI-PUBLICLY-23H04403
  • [Presentation] 分子から生命が生まれる道筋を解き明かすために2023

    • Author(s)
      水内良
    • Organizer
      生命の分子システムの理解に向けて何を創れば良いか?
    • Invited
    • Data Source
      KAKENHI-PROJECT-23K14153
  • [Presentation] Evolution of complexity in an artificial RNA replication system2023

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      STATPHYS28 Satellite Meeting, Statistical Physics and Information-Processing in Living Systems
    • Invited / Int'l Joint Research
    • Data Source
      KAKENHI-PUBLICLY-23H04403
  • [Presentation] Experimental evolution of artificial RNA replication systems: for understanding the origins of life2023

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      International Workshop for Bridging Synthetic, Computational & Evolutionary Biology
    • Invited / Int'l Joint Research
    • Data Source
      KAKENHI-PUBLICLY-23H04403
  • [Presentation] 再構成実験で探る生命の起源:原始生命モデルと実験室内進化2023

    • Author(s)
      水内良
    • Organizer
      CHEMINAS 47
    • Invited
    • Data Source
      KAKENHI-PROJECT-23K14153
  • [Presentation] Minimal RNA self-reproduction discovered from a random pool of oligomers2023

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      第61回日本生物物理学会年会
    • Invited
    • Data Source
      KAKENHI-PROJECT-23K14153
  • [Presentation] RNA replication systems and evolution: for understanding the origins of life2023

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      SPEED × Bottom-up Biotech × ELSI joint workshop
    • Invited / Int'l Joint Research
    • Data Source
      KAKENHI-PUBLICLY-23H04403
  • [Presentation] 原始的なRNA集団の調査から見つかった自己複製する最小のRNA2023

    • Author(s)
      水内良、市橋伯一
    • Organizer
      「細胞を創る」研究会 16.0
    • Data Source
      KAKENHI-PUBLICLY-23H04403
  • [Presentation] Evolution of complexity in an artificial RNA replication system2023

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      STATPHYS28 Satellite Meeting, Statistical Physics and Information-Processing in Living Systems
    • Invited / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-23K14153
  • [Presentation] 分子から生命が生まれる道筋を解き明かすために2023

    • Author(s)
      水内良
    • Organizer
      生命の分子システムの理解に向けて何を創れば良いか?
    • Invited
    • Data Source
      KAKENHI-PUBLICLY-23H04403
  • [Presentation] 再構成実験で探る生命の起源:原始生命モデルと実験室内進化2023

    • Author(s)
      水内良
    • Organizer
      CHEMINAS 47
    • Invited
    • Data Source
      KAKENHI-PUBLICLY-23H04403
  • [Presentation] Experimental evolution of artificial RNA replication systems: for understanding the origins of life2023

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      International Workshop for Bridging Synthetic, Computational & Evolutionary Biology
    • Invited / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-23K14153
  • [Presentation] 人工RNA複製システムと生命の起源2022

    • Author(s)
      水内良
    • Organizer
      学術変革領域(A)分子サイバネティクス 第10回領域セミナー
    • Invited
    • Data Source
      KAKENHI-PUBLICLY-21H05867
  • [Presentation] Compartmentalization for RNA replication and evolution2022

    • Author(s)
      水内良
    • Organizer
      第60回日本生物物理学会年会
    • Invited
    • Data Source
      KAKENHI-PUBLICLY-21H05867
  • [Presentation] From emergence to Darwinian evolution of RNA replicators2022

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      NASA Center for the Origin of Life meeting
    • Invited / Int'l Joint Research
    • Data Source
      KAKENHI-PUBLICLY-21H05867
  • [Presentation] Emergence and Darwinian evolution of RNA replicators2022

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      Interdisciplinary Origin of Life Meeting for Early Career Researchers (IOoL2022)
    • Int'l Joint Research
    • Data Source
      KAKENHI-PUBLICLY-21H05867
  • [Presentation] 人工RNA複製体のダーウィン進化による複雑化2022

    • Author(s)
      水内良、古林太郎、市橋伯一
    • Organizer
      第46回 生命の起原および進化学会学術講演会
    • Data Source
      KAKENHI-PUBLICLY-21H05867
  • [Presentation] Mineral surfaces select for longer RNA molecules: implication for prebiotic evolution2022

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      Japan Geoscience Union Meeting
    • Invited / Int'l Joint Research
    • Data Source
      KAKENHI-PUBLICLY-21H05867
  • [Presentation] 安定化した液滴での翻訳とRNA自己複製:人工多細胞の構築に向けて2022

    • Author(s)
      水内良、市橋伯一
    • Organizer
      「細胞を創る」研究会 15.0
    • Data Source
      KAKENHI-PUBLICLY-21H05867
  • [Presentation] 人工RNA自己複製体と寄生体の長期共進化2021

    • Author(s)
      水内良
    • Organizer
      日本微生物生態学会第34回大会
    • Invited
    • Data Source
      KAKENHI-PUBLICLY-21H05867
  • [Presentation] 原始生命を模した分子複製システムの進化と改良2021

    • Author(s)
      水内良
    • Organizer
      生命の起原および進化学会 2021年シンポジウム
    • Invited
    • Data Source
      KAKENHI-PROJECT-19K23763
  • [Presentation] Evolutionary complexification of an artificial RNA replication system2021

    • Author(s)
      Ryo Mizuuchi, Taro Furubayashi, Norikazu Ichihashi
    • Organizer
      第59回日本生物物理学会年会
    • Data Source
      KAKENHI-PUBLICLY-21H05867
  • [Presentation] 人工RNA複製体の進化と人工細胞と生命の起源2021

    • Author(s)
      水内良
    • Organizer
      人工細胞モデル&分子ロボティクス 第2回研究会
    • Invited
    • Data Source
      KAKENHI-PUBLICLY-21H05867
  • [Presentation] 原始生命を模した分子複製システムの構築と進化2021

    • Author(s)
      水内良
    • Organizer
      第1回分子サイバネティクス定例研究会
    • Invited
    • Data Source
      KAKENHI-PROJECT-19K23763
  • [Presentation] 無細胞翻訳系を用いた液滴内でのRNAの自己複製2021

    • Author(s)
      水内良、市橋伯一
    • Organizer
      第15回無細胞生命科学研究会
    • Data Source
      KAKENHI-PROJECT-19K23763
  • [Presentation] Evolutionary complexification of a replicase-encoding RNA replicator2021

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      The XIXth International Conference on the Origin of Life
    • Invited / Int'l Joint Research
    • Data Source
      KAKENHI-PUBLICLY-21H05867
  • [Presentation] Evolutionary complexification of an artificial RNA replication system in a cell-like compartment2021

    • Author(s)
      Ryo Mizuuchi、Taro Furubayashi、Norikazu Ichihashi
    • Organizer
      「細胞を創る」研究会 14.0
    • Data Source
      KAKENHI-PUBLICLY-21H05867
  • [Presentation] Emergence of cooperative RNA replicators in an artificial cell-like system2020

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      8th ELSI Symposium
    • Data Source
      KAKENHI-PROJECT-19K23763
  • [Presentation] Translation-coupled RNA replication in membrane-free droplets2020

    • Author(s)
      水内良、市橋伯一
    • Organizer
      第58回日本生物物理学会年会
    • Data Source
      KAKENHI-PROJECT-19K23763
  • [Presentation] 液滴での翻訳を介したRNAの自己複製2020

    • Author(s)
      水内良、市橋伯一
    • Organizer
      「細胞を創る」研究会 13.0
    • Data Source
      KAKENHI-PROJECT-19K23763
  • [Presentation] RNA自己複製体のダーウィン進化による複雑化2020

    • Author(s)
      水内良、古林太郎、市橋伯一
    • Organizer
      日本進化学会 第22回大会
    • Data Source
      KAKENHI-PROJECT-19K23763
  • [Presentation] Autocatalytic sets, Mineral surfaces, and the evolution of RNA2019

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      Ribonucleoprotein reconstruction/evolution mini-workshop
    • Invited / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-19K23763
  • [Presentation] Compartmentalization for the evolution of cooperation in RNA replicators2019

    • Author(s)
      Ryo Mizuuchi
    • Organizer
      Primitive Compartmentalization Symposium
    • Invited / Int'l Joint Research
    • Data Source
      KAKENHI-PROJECT-19K23763
  • 1.  ICHIHASHI Norikazu
    # of Collaborated Projects: 0 results
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