MIYOSHI Kazuchika 三好和睦

Researcher Number	70363611
Other IDs
Affiliation (Current)	2025: 鹿児島大学, 農水産獣医学域獣医学系, 教授
Affiliation (based on the past Project Information) *help	2015 – 2022: 鹿児島大学, 農水産獣医学域農学系, 教授 2014: 鹿児島大学農学部, 生物生産学科, 教授 2007 – 2014: Kagoshima University, 農学部, 准教授 2004 – 2006: 鹿児島大学, 農学部, 助教授
Review Section/Research Field	Principal Investigator Applied animal science / Integrative animal science / Basic Section 42040:Laboratory animal science-related Except Principal Investigator Applied animal science / Basic Section 42020:Veterinary medical science-related / Basic Section 42010:Animal production science-related / Experimental pathology / Animal production science
Keywords	Principal Investigator ミニブタ / 核移植 / 体細胞クローニング / マイクロミニピッグ / 体細胞核移植 / 遺伝子ノックアウト動物 / 胚盤胞 / ブタ / 生殖細胞 / 凍結保存 … More / エレクトロポレーション / 疾患モデル動物 / リプログラミング / 4遺伝子 / Oct-3 / 遺伝子改変 … More Except Principal Investigator 体細胞核移植 / 遺伝子改変 / マイクロミニブタ / クローンブタ / ブタ / 動脈硬化 / ゲノム編集 / α-Gal epitope / 遺伝子導入 / 胎仔性繊維芽細胞 / 体細胞クローン / 抗がん剤（メトトレキサート） / 腫瘍循環器学 / 抗がん剤 / メトトレキサート / 抗がん剤メトトレキサート / 葉酸代謝 / 脂質代謝 / 腫瘍循環器病学 / メタボリックシンドローム / 高コレステロール血症 / ミニブタ / 遺伝子工学 / エピゲノム修飾 / メチル化転移酵素 / メチル化 / ヒストン / DNAメチル化転移酵素1 / 初期化 / EGFP / Oct-3/4 / DNAメチル化転移酵素1遺伝子 / DNAメチル化 / 遺伝子改変動物 / 疾患モデル動物 / クローン / マイクロミニピッグ / 受精卵遺伝子改変 / 受精卵採取 / アルツハイマー病 / クローン動物 / 動物モデル / ＬＤＬＲ / LDLR / isolectin IB4 / genome editing / targeted toxin / CRISPR/Cas9 / GAAT1 / トロンボモジュリン / DAF / CD47 / EndoGalC / α-Galエピトープ / ブタ細胞 / 異種移植拒絶関連遺伝子 / thrombomodulin（血液凝固阻害因子） / DAF（補体抑制因子） / 異種移植 / ＫＯ / ホセKOブタ / gene targetin / ホモKOブタ / double KO / 薬剤耐性 / gene targeting / ホモ / KOブタ / バルプロ酸 / 発生 / クラウン系ミニブタ / ラトランキュリンA / スクリパタイド / スベロイルビスヒドロキサム酸 / トリコスタチンA / 脱アセチル化 Less

Mechanism elucidation of methotrexate arteriosclerosis exacerbation with human atherosclerosis pig model
- Principal Investigator
  
  Kawaguchi Hiroaki
- Project Period (FY)
  2020 – 2022
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 42020:Veterinary medical science-related
- Research Institution
  Kitasato University
  Kagoshima University
Generation of gene knockout microminipigs based on the modification process to somatic cell nuclear transfer embryosPrincipal Investigator
- Principal Investigator
  
  Miyoshi Kazuchika
- Project Period (FY)
  2020 – 2022
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 42040:Laboratory animal science-related
- Research Institution
  Kagoshima University
Attempts to perform genome modification towards donor cells for improving SCNT efficiency in pigs
- Principal Investigator
  
  Sato Masahiro
- Project Period (FY)
  2019 – 2021
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 42010:Animal production science-related
- Research Institution
  National Center for Child Health and Development
  Kagoshima University
Development of genetically modified microminipigs that can contribute to cancer treatments as disease model animalsPrincipal Investigator
- Principal Investigator
  
  MIYOSHI Kazuchika
- Project Period (FY)
  2016 – 2018
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Integrative animal science
- Research Institution
  Kagoshima University
Production of gene-modified pig of next-generation model for Alzheimer's disase
- Principal Investigator
  
  Tanimoto Akihide
- Project Period (FY)
  2016 – 2018
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Experimental pathology
- Research Institution
  Kagoshima University
Efficient enrichment of homozygous bi-allelic knockout microminiature porcine cells using a novel selection system and production of genome-edited cloned piglets
- Principal Investigator
  
  SATO Masahiro
- Project Period (FY)
  2015 – 2017
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Animal production science
- Research Institution
  Kagoshima University
Establishment of somatic cell nuclear transfer techniques in Microminipigs, the smallest pigs in the worldPrincipal Investigator
- Principal Investigator
  
  MIYOSHI KAZUCHIKA
- Project Period (FY)
  2013 – 2015
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Integrative animal science
- Research Institution
  Kagoshima University
Production of cloned genetically modified pigs by a new drug-free gene-modified cell isolation system
- Principal Investigator
  
  SATO Masahiro
- Project Period (FY)
  2012 – 2014
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Applied animal science
- Research Institution
  Kagoshima University
Improvement of somatic cell nuclear transfer techniques in miniature pigs using a noninvasive evaluation system for reprogramming statusPrincipal Investigator
- Principal Investigator
  
  MIYOSHI Kazuchika
- Project Period (FY)
  2010 – 2012
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Applied animal science
- Research Institution
  Kagoshima University
Development of a general method for obtaining double KO swine cells which will be used as donors for production of cloned homozygous piglets
- Principal Investigator
  
  SATO Masahiro
- Project Period (FY)
  2009 – 2011
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Applied animal science
- Research Institution
  Kagoshima University
Improvement of the developmental capacity of nuclear transfer embryos derived from gene-modified clawn miniature pig cells using a histone deacetylase inhibitor and latrunculin A.
- Principal Investigator
  
  YOSHIDA Mitsutoshi
- Project Period (FY)
  2008 – 2010
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Applied animal science
- Research Institution
  Kagoshima University
Development of a noninvasive evaluation system for reprogramming status in miniature pig somatic cell nuclear transfer embryosPrincipal Investigator
- Principal Investigator
  
  MIYOSHI Kazuchika
- Project Period (FY)
  2007 – 2008
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Applied animal science
- Research Institution
  Kagoshima University
体細胞核移植による効率的なクローンミニブタ作出システムの確立Principal Investigator
- Principal Investigator
  
  三好和睦
- Project Period (FY)
  2004 – 2006
- Research Category
  
  Grant-in-Aid for Young Scientists (A)
- Research Field
  
  Applied animal science
- Research Institution
  Kagoshima University

[Book] Targeted toxin as a useful reagent for enrichment of x-Gal epitope-negative cells used for somatic cell nuclear transfer in pigs2012
- Author(s)
  Sato M, Chi H, Miyoshi K. In Tech
- Publisher
  Xenotransplantation
- Data Source
  KAKENHI-PROJECT-22580321
[Book] Cell Reprogramming and Transgenesis by Nuclear Transfer2005
- Author(s)
  Kazuchika Miyoshi
- Publisher
  Humana Press, Totowa, USA(印刷中)
- Data Source
  KAKENHI-PROJECT-16688008
[Journal Article] Improved fertility of frozen‐thawed porcine spermatozoa with 3,3‐dimethylglutaric anhydride poly‐L‐lysine as a novel cryoprotectant2023
- Author(s)
  Jin Hansol、Choi Wooyoon、Matsumura Kazuaki、Hyon Suong‐hyu、Gen Yuki、Hayashi Masakazu、Kawabata Tadahiro、Ijiri Moe、Miyoshi Kazuchika
- Journal Title
  
  Animal Science Journal
  
  Volume: 94 Issue: 1
- DOI
  10.1111/asj.13821
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PUBLICLY-21H05516, KAKENHI-PROJECT-20H04532, KAKENHI-PROJECT-20K06472
[Journal Article] Cytoplasmic microinjection of piggyBac transposase mRNA and transposon vectors for efficient in vitro production of transgenic porcine parthenotes.2022
- Author(s)
  Miyagasako R、Hansol J、Watanabe S、Miyoshi K、Inada E、Sato M
- Journal Title
  
  OBM Genetics
  
  Volume: 06 Issue: 03 Pages: 1-20
- DOI
  10.21926/obm.genet.2203166
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-21K10165, KAKENHI-PROJECT-20K06472, KAKENHI-PROJECT-20K10210
[Journal Article] Cryopreservation of pig spermatozoa using carboxylated poly-L-lysine as cryoprotectant2022
- Author(s)
  Jin Hansol, Choi Wooyoon, Matsumura Kazuaki, Hyon Suong-hyu, Gen Yuki, Hayashi Masakazu, Kawabata Tadahiro, Ijiri Moe, Miyoshi Kazuchika
- Journal Title
  
  J. Reprod. Dev.
  
  Volume: 68 Issue: 5 Pages: 312-317
- DOI
  10.1262/jrd.2022-058
- ISSN
  0916-8818, 1348-4400
- Language
  English
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K06472
[Journal Article] In vitro Electroporation in the Presence of CRISPR/Cas9 Reagents as a Safe and Effective Method for Producing Biallelic Knock-Out Porcine Embryos2021
- Author(s)
  Sato Masahiro, Jin Hansol, Akasaka Eri, Miyoshi Kazuchika
- Journal Title
  
  OBM Genetics
  
  Volume: 5 Issue: 1 Pages: 15-15
- DOI
  10.21926/obm.genet.2101123
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-20K06472, KAKENHI-PROJECT-19K06372
[Journal Article] Production of genetically engineered mice with higher efficiency, lower mosaicism, and multiplexing capability using maternally expressed Cas92020
- Author(s)
  Sakurai T, Kamiyoshi A, Kawate H, Watanabe S, Sato M, Shindo T
- Journal Title
  
  Sci Rep
  
  Volume: 10(1) Issue: 1 Pages: 1091-1091
- DOI
  10.1038/s41598-020-57996-7
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K07317, KAKENHI-PROJECT-19K07477, KAKENHI-PROJECT-19K06372
[Journal Article] Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase2019
- Author(s)
  Sakurai Takayuki、Kamiyoshi Akiko、Takei Norio、Watanabe Satoshi、Sato Masahiro、Shindo Takayuki
- Journal Title
  
  Scientific Reports
  
  Volume: 9 Issue: 1 Pages: 9923-9923
- DOI
  10.1038/s41598-019-46357-8
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-18K07060, KAKENHI-PROJECT-19K06372, KAKENHI-PROJECT-19K07477, KAKENHI-PROJECT-18H02602
[Journal Article] Recent Advance in Genome Editing-Based Gene Modification in Pigs2019
- Author(s)
  Sato Masahiro、Miyoshi Kazuchika、Kawaguchi Hiroaki、Inada Emi、Saitoh Issei、Tanimoto Akihide
- Journal Title
  
  Reproductive Biology and Technology in Animals (Edited by: Juan Carlos Garon Poggi and Katy Satue Ambrojo) (InTechOpen)
  
  Volume: - Pages: 1-35
- DOI
  10.5772/intechopen.88022
- ISBN
  9781789841794, 9781789841800
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-19K06372
[Journal Article] The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones2018
- Author(s)
  Watanabe Satoshi、Sakurai Takayuki、Nakamura Shingo、Miyoshi Kazuchika、Sato Masahiro
- Journal Title
  
  International Journal of Molecular Sciences
  
  Volume: 19 Issue: 4 Pages: 1075-1075
- DOI
  10.3390/ijms19041075
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-16H05049, KAKENHI-PROJECT-16H05176, KAKENHI-PROJECT-16K08085
[Journal Article] Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes2018
- Author(s)
  Sato M, Maeda K, Koriyama M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai, T, Watanabe S, Miyoshi K
- Journal Title
  
  Theriogenology
  
  Volume: 108 Pages: 29-38
- DOI
  10.1016/j.theriogenology.2017.11.030
- NAID
  120007099790
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-16K08085, KAKENHI-PROJECT-16K15063, KAKENHI-PROJECT-16H05049, KAKENHI-PROJECT-16K15233, KAKENHI-PROJECT-18K09839, KAKENHI-PROJECT-16H05176, KAKENHI-PROJECT-16K11810
[Journal Article] Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.2017
- Author(s)
  Sato M, Miyoshi K, Nakamura S, Ohtsuka M, Sakurai T, Watanabe S, Kawaguchi H, Tanimoto A.
- Journal Title
  
  Int J Mol Sci
  
  Volume: 18 Issue: 12 Pages: 2610-2610
- DOI
  10.3390/ijms18122610
- NAID
  120007099791
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-16K08023, KAKENHI-PROJECT-16K08085, KAKENHI-PROJECT-16K11810, KAKENHI-PROJECT-16K15063, KAKENHI-PROJECT-16H05049, KAKENHI-PROJECT-16H05176, KAKENHI-PROJECT-15K07695, KAKENHI-PROJECT-16K15233
[Journal Article] Hypertension alters the endothelial-dependent biphasic response of bradykinin in isolated Microminipig basilar artery.2017
- Author(s)
  Zahorul Islam M, Kawaguchi H, Miura N, Miyoshi N, Yamazaki-Himeno E, Shiraishi M, Miyamoto A, Tanimoto A.
- Journal Title
  
  Microvascular Research
  
  Volume: 114 Pages: 52-57
- DOI
  10.1016/j.mvr.2017.06.001
- Peer Reviewed / Open Access / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16H05176, KAKENHI-PROJECT-16H05845
[Journal Article] Selection of human blastocysts with a high implantation potential based on timely compaction2017
- Author(s)
  Mizobe Y, Ezono Y, Tokunaga M, Oya N, Iwakiri R, Yoshida N, Sato Y, Onoue N, Miyoshi K
- Journal Title
  
  Journal of Assisted Reproduction and Genetics
  
  Volume: 34 Issue: 8 Pages: 991-997
- DOI
  10.1007/s10815-017-0962-y
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16K08085
[Journal Article] Effects of early cleavage patterns of human embryos on subsequent in vitro development and implantation2016
- Author(s)
  Mizobe Y, Oya N, Iwakiri R, Yoshida N, Sato Y, Miyoshi K, Tokunaga M, Ezono Y
- Journal Title
  
  Fertility and Sterility
  
  Volume: 106 Issue: 2 Pages: 348-353
- DOI
  10.1016/j.fertnstert.2016.04.020
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16K08085
[Journal Article] A non-inheritable maternal Cas9-based multiple-gene editing system in mice2016
- Author(s)
  Sakurai T, Kamiyoshi A, Kawate H, Mori C, Watanabe S, Tanaka M, Uetake R, Sato M, Shindo T.
- Journal Title
  
  Sci Rep
  
  Volume: 6 Issue: 1 Pages: 20011-20011
- DOI
  10.1038/srep20011
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-25460330, KAKENHI-PROJECT-26670153, KAKENHI-PROJECT-26293085, KAKENHI-PROJECT-26293183, KAKENHI-PROJECT-26450454, KAKENHI-PROJECT-15K07695
[Journal Article] Development of a culture medium for rat 1-cell embryos2016
- Author(s)
  Miyoshi K
- Journal Title
  
  Journal of Mammalian Ova Research
  
  Volume: 33 Issue: 1 Pages: 11-16
- DOI
  10.1274/jmor.33.11
- NAID
  40020876145
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16K08085
[Journal Article] Birth of Cloned Microminipigs Derived from Somatic Cell Nuclear Transfer Embryos that have been Transiently Treated with Valproic Acid.2016
- Author(s)
  Miyoshi K, Maeda K, Akioka K, Sato M, Kawaguchi H, Tanimoto A
- Journal Title
  
  Cellular Reprogramming
  
  Volume: 6 Issue: 6 Pages: 390-400
- DOI
  10.1089/cell.2016.0025
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-15K07695, KAKENHI-PROJECT-16K08085, KAKENHI-PROJECT-16H05176
[Journal Article] The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs.2016
- Author(s)
  Sato M, Maeda K, Koriyama M, Inada E, Saitoh I, Miura H, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K.
- Journal Title
  
  Int J Mol Sci.
  
  Volume: 17 Issue: 9 Pages: 1424-1424
- DOI
  10.3390/ijms17091424
- NAID
  130007023583
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-16H05049, KAKENHI-PROJECT-25293418, KAKENHI-PROJECT-25463192, KAKENHI-PROJECT-15K07695, KAKENHI-PROJECT-16K08085, KAKENHI-PROJECT-16K11810, KAKENHI-PROJECT-15K14371, KAKENHI-PROJECT-16K15063
[Journal Article] Developmental ability of embryos produced from oocytes with fragile oolemma by intracytoplasmic sperm injection2016
- Author(s)
  Mizobe Y, Oya N, Iwakiri R, Yoshida N, Sato Y, Onoue N, Miyoshi K, Tokunaga M, Ezono Y
- Journal Title
  
  Journal of Assisted Reproduction and Genetics
  
  Volume: 33 Issue: 12 Pages: 1685-1690
- DOI
  10.1007/s10815-016-0811-4
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-16K08085
[Journal Article] Direct injection of CRISPR/Cas9-related mRNA into cytoplasm of parthenogenetically activated porcine oocytes causes frequent mosaicism for indel mutations.2015
- Author(s)
  Sato M, Koriyama M, Watanabe S, Ohtsuka M, Sakurai T, Inada E, Saitoh I, Nakamura S, Miyoshi K
- Journal Title
  
  International Journal of Molecular Sciences,
  
  Volume: 16 Issue: 8 Pages: 17838-17856
- DOI
  10.3390/ijms160817838
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-15K07695, KAKENHI-PROJECT-25463192, KAKENHI-PROJECT-25450475, KAKENHI-PROJECT-26670153, KAKENHI-PROJECT-25850226, KAKENHI-PROJECT-26293085, KAKENHI-PROJECT-26450454
[Journal Article] A new rolling culture-based in vitro fertilization system capable of reducing polyspermy in porcine oocytes2015
- Author(s)
  Kitaji H, Ookutsu S, Sato M, Miyoshi K
- Journal Title
  
  Animal Science Journal
  
  Volume: 86 Issue: 5 Pages: 494-498
- DOI
  10.1111/asj.12327
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-24580411, KAKENHI-PROJECT-25450475
[Journal Article] Generation of α-1,3-galactosyltransferase-deficient porcine embryonic fibroblasts by CRISPR/Cas9-mediated knock-in of a small mutated sequence and a targeted toxin-based selection system.2015
- Author(s)
  Sato M, Kagoshima A, Saitoh I, Inada E, Miyoshi K, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
- Journal Title
  
  Domestic Animal Reproduction
  
  Volume: 50 Issue: 5 Pages: 872-880
- DOI
  10.1111/rda.12565
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-15K07695, KAKENHI-PROJECT-15K11362, KAKENHI-PROJECT-25463192, KAKENHI-PROJECT-25450475, KAKENHI-PROJECT-26670153, KAKENHI-PROJECT-25850226, KAKENHI-PROJECT-25293418, KAKENHI-PROJECT-26293085, KAKENHI-PROJECT-26450454
[Journal Article] Production of cloned miniature pigs expressing high levels of human apolipoprotein(a) in plasma2015
- Author(s)
  Ozawa M, Himaki T, Ookutsu S, Mizobe Y, Ogawa J, Miyoshi K, Yabuki A, Fan J, Yoshida M
- Journal Title
  
  PLoS ONE
  
  Volume: 10 Issue: 7 Pages: e0132155-e0132155
- DOI
  10.1371/journal.pone.0132155
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-25450475
[Journal Article] Preincubation with green tea polyphenol extract is beneficial for attenuating sperm injury caused by freezing-thawing in swine.2015
- Author(s)
  Kitaji H, Ookutsu S, Sato M, Miyoshi K
- Journal Title
  
  Animal Science Journal
  
  Volume: 86 Issue: 11 Pages: 922-928
- DOI
  10.1111/asj.12379
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-15K07695, KAKENHI-PROJECT-25450475
[Journal Article] The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of theα-1,3-galactosyltransferase gene in porcine embryonic fibroblasts2014
- Author(s)
  Sato M, Miyoshi K, Nagao Y, Nishi Y, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
- Journal Title
  
  Xenotransplantation
  
  Volume: 21 FEB( Article first published online) Issue: 3 Pages: 291-300
- DOI
  10.1111/xen.12089
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24580411, KAKENHI-PROJECT-26293085, KAKENHI-PROJECT-26670153
[Journal Article] A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice2014
- Author(s)
  Sakurai T, Watanabe S, Kamiyoshi A, Sato M, Shindo T
- Journal Title
  
  BMC Biotechnology
  
  Volume: 14 Issue: 1 Pages: 69-69
- DOI
  10.1186/1472-6750-14-69
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-24580411, KAKENHI-PROJECT-25460330, KAKENHI-PROJECT-26293085, KAKENHI-PROJECT-26293183, KAKENHI-PROJECT-26670153
[Journal Article] Osmolarity- and stage-dependent effects of glycine on parthenogenetic development of pig oocytes2014
- Author(s)
  Miyoshi K, Mizobe Y
- Journal Title
  
  Journal of Reproduction and Development
  
  Volume: 60 Pages: 349-354
- NAID
  130004137829
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-25450475
[Journal Article] The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the α-1,3-galactosyltransferase gene in porcine embryonic fibroblasts2014
- Author(s)
  Sato M, Miyoshi K, Nagao Y, Nishi Y, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
- Journal Title
  
  Xenotransplantation
  
  Volume: 21
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-25450475
[Journal Article] Successful Suppression of Endogenous <i>α-1,3-Galactosyltransferase</i> Expression by RNA Interference in Pig Embryos Generated <i>In Vitro</i>2012
- Author(s)
  Chi H
- Journal Title
  
  J. Reprod. Dev.
  
  Volume: 58 Issue: 1 Pages: 69-76
- DOI
  10.1262/jrd.10-165M
- NAID
  130001170088
- ISSN
  0916-8818, 1348-4400
- Language
  English
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22580321, KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24580411
[Journal Article] Successful suppression of endogenous α-1, 3-galactosyltransferase expression by RNA interference in pig embryos generated in vitro2012
- Author(s)
  Chi H, Shinohara M, Yokomine T, Sato M, Takao S, Yoshida M, Miyoshi K
- Journal Title
  
  Journal of Reproduction and Developmen
  
  Volume: 58 Pages: 69-76
- NAID
  10030407205
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Effect of postactivation treatment with latrunculin A on in vitro and in vivo development of cloned embryos derived from kidney fibroblasts of an aged Clawn miniature boar2012
- Author(s)
  Himaki T, Mizobe Y, Tsuda K, Suetomo M, Yamakuchi H, Miyoshi K, Takao S, Yoshida M
- Journal Title
  
  Journal of Reproduction and Development
  
  Volume: 58 Pages: 398-403
- NAID
  10031066746
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22580321
[Journal Article] Successful suppression of endogenous α-1, 3-galactosyltransferase expression by RNA interference in pig embryos generated in vitro2012
- Author(s)
  Chi H, Shinohara M, Yokomine T, Sato M, Takao S, Yoshida M, Miyoshi K.
- Journal Title
  
  J Reprod Dev
  
  Volume: 58(1) Pages: 69-76
- NAID
  10030407205
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Determination of the optimal concentration of several selective drugs useful for generating multi-transgenic porcine embryonic fibroblasts2012
- Author(s)
  Sato M, Ohtsuka M, Miura H, Miyoshi K, Watanabe S
- Journal Title
  
  Reproduction in Domestic Animals
  
  Volume: (in press)
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Expression analysis of an α-1,3-galactosyltransferase, an enzyme that creates xenotransplantation-related α-Gal epitope, in pig preimplantation embryos2012
- Author(s)
  Chi H
- Journal Title
  
  Animal Science Journal
  
  Volume: 83 Issue: 1 Pages: 88-93
- DOI
  10.1111/j.1740-0929.2011.00964.x
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22580321, KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24580411
[Journal Article] Determination of the Optimal Concentration of Several Selective Drugs Useful for Generating Multi-Transgenic Porcine Embryonic Fibroblasts2012
- Author(s)
  Sato M, Ohtsuka M, Miura H, Miyoshi K, Watanabe S.
- Journal Title
  
  Reproduction in Domestic Animals/John Wiley & Sons, Inc.
  
  Volume: 47 Issue: 5 Pages: 759-765
- DOI
  10.1111/j.1439-0531.2011.01964.x
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350, KAKENHI-PROJECT-22580321, KAKENHI-PROJECT-23592892, KAKENHI-PROJECT-23593038, KAKENHI-PROJECT-23700514, KAKENHI-PROJECT-23700562, KAKENHI-PROJECT-24580411, KAKENHI-PROJECT-24700435, KAKENHI-PLANNED-22133002
[Journal Article] Expression analysis of a α-1,3-galactosyltransferase, an enzyme that creates xenotransplantation-related α-Gal epitope, in pig preimplantation embryos2012
- Author(s)
  Chi H, Sato M, Yoshida M, Miyoshi K
- Journal Title
  
  Animal Science Journal
  
  Volume: 83 Pages: 88-93
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Cultivation with untransfected fibroblasts can stimulate proliferation of a single gene-modified fibroblast derived from a Clawn miniature swine fetus2011
- Author(s)
  Sato M, Yoshida M, Miyoshi K, Ohtsuka M, Watanabe S
- Journal Title
  
  Reproduction in Domestic Animals
  
  Volume: 46 Pages: 911-916
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Targeted toxin as a useful reagent for enrichment of α-Gal epitope-negative cells used for somatic cell nuclear transfer in pigs2011
- Author(s)
  Sato M, Chi H, Miyoshi K
- Journal Title
  
  Xenotransplantation/In Tech
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Cultivation with untransfected fibroblasts can stimulate proliferation of a single gene-modified fibroblast derived from a Clawn miniature swine fetus2011
- Author(s)
  Sato M, Yoshida M, Miyoshi K, Ohtsuka M, Watanabe S
- Journal Title
  
  Reproduction in Domestic Animals/John Wiley & Sons, Inc.
  
  Volume: Vol.46, No.5 Pages: 911-916
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Whole-genome amplification-based GenomiPhi for multiple genomic analysis of individual early porcine embryos2011
- Author(s)
  Akasaka E, Ozawa A, Mori H, Mizobe Y, Yoshida M, Miyoshi K, Sato M.
- Journal Title
  
  Theriogenology/Elsevier B. V.
  
  Volume: 75(8)
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Targeted Toxin as a Useful Reagent for Enrichment ofα-Gal Epitope-Negative Cells Used for Somatic Cell Nuclear Transfer in Pigs2011
- Author(s)
  Sato M, Chi H, Miyoshi K
- Journal Title
  
  Xenotransplantation(InTech Open Acess Book)
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Whole-genome amplification-based GenomiPhi for multiple genomic analys is of individual early porcine embryos2011
- Author(s)
  Akasaka E, Ozawa A, Mori H, Mizobe Y, Yoshida M, Miyoshi K, Sato M
- Journal Title
  
  Theriogenology/Elsevier B.V.
  
  Volume: 75 Pages: 1543-1549
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Beneficial effects of reversine on in vitro development of miniature pig somatic cell nuclear transfer embryos2010
- Author(s)
  Miyoshi K, Mori H, Mizobe Y, Himaki T, Yoshida M, Sato M.
- Journal Title
  
  Journal of Reproduction and Development
  
  Volume: 56巻 Pages: 291-296
- NAID
  10026324772
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Enrichment of xenograft-competent genetically modified pig cells using a targeted toxin, isolectin BS-I-B4 conjugate2010
- Author(s)
  Akasaka E, Watanabe S, Himaki T, Ohtsuka M, Yoshida M, Miyoshi K, Sato M.
- Journal Title
  
  Xenotransplantation
  
  Volume: 17巻 Pages: 81-89
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Latrunculin A dramatically improves the developmental capacity of nuclear tramsfer embryos derived from gene-modified Clawn miniature pig cells2010
- Author(s)
  Himaki T, Mori H, Mizobe Y, Miyoshi K, Sato M, Takao S, Yoshida M.
- Journal Title
  
  Cellular Reprogramming
  
  Volume: 12巻 Pages: 127-131
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Valproic acid enhances in vitro development and Oct-3/4 expression of miniature pig somatic cell nuclear transfer embryos2010
- Author(s)
  Miyoshi K, Mori H, Mizobe Y, Akasaka E, Ozawa A, Yoshida M, Sato M.
- Journal Title
  
  Cellular Reprogramming
  
  Volume: 12巻 Pages: 67-74
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Beneficial effects of reversine on in vitro development of miniature pig somatic cell nuclear transfer embryos2010
- Author(s)
  Miyoshi K, Mori H, Mizobe Y, Himaki T, Yoshida M, Sato M
- Journal Title
  
  Journal of Reproduction and Development
  
  Volume: 56 Pages: 291-296
- NAID
  10026324772
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22580321
[Journal Article] Production of genetically modified porcine blastocysts by somatic cell nuclear transfer : preliminary results toward production of xenograft-competent miniature pigs2010
- Author(s)
  Himaki T, Watanabe S, Chi H, Yoshida M, Miyoshi K, Sato M
- Journal Title
  
  J Reprod Dev
  
  Volume: Vol.56 Pages: 630-638
- NAID
  10027730311
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580311
[Journal Article] Effects of trichostatin A on in vitro development and transgene function in somatic cell nuclear transfer embryos derived from transgenic Clawn miniature pig cells2010
- Author(s)
  Himaki T, Yokomine TA, Sato M, Takao S, Miyoshi K, Yoshida M
- Journal Title
  
  Anim Sci J
  
  Volume: 81 Pages: 558-563
- NAID
  10027889588
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580311
[Journal Article] Latrunculin A dramatically improves the developmental capacity of nuclear tramsfer embryos derived from gene-modified Clawn miniature pig cells2010
- Author(s)
  Himaki T, Mori H, Mizobe Y, Miyoshi K, Sato M, Takao S, Yoshida M
- Journal Title
  
  Cellular Reprogramming
  
  Volume: 12 Issue: 2 Pages: 127-131
- DOI
  10.1089/cell.2009.0066
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22580321
[Journal Article] Effects of trichostatin A on in vitro development and transgene function in somatic cell nuclear transfer embryos derived from transgenic Clawn miniature pig cells2010
- Author(s)
  Himaki T, Yokomine T, Sato M, Takao S, Miyoshi K, Yoshida M
- Journal Title
  
  Animal Science Journal
  
  Volume: 81 Issue: 5 Pages: 558-563
- DOI
  10.1111/j.1740-0929.2010.00772.x
- NAID
  10027889588
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22580321
[Journal Article] Effects of trichostatin A on in vitro development and transgene function in somatic cell nuclear transfer embryos derived from transgenic Clawn miniature pig cells2010
- Author(s)
  Himaki T, Yokomine T, Sato M, Miyoshi K, Takao S, Yoshida M.
- Journal Title
  
  Animal Science Journal
  
  Volume: 81巻 Pages: 558-563
- NAID
  10027889588
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Production of genetically modified porcine blastocysts by somatic cell nuclear transfer : preliminary results toward production of xenograft-competent miniature pigs2010
- Author(s)
  Himaki T, Watanabe S, Chi H, Yoshida M, Miyoshi K, Sato M.
- Journal Title
  
  Journal of Reproduction and Development
  
  Volume: 56巻 Pages: 630-638
- NAID
  10027730311
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Valproic acid enhances in vitro development and Oct-3/4 expression of miniature pig somatic cell nuclear transfer embryos2010
- Author(s)
  Miyoshi K, Mori H, Mizobe Y, Akasaka E, Ozawa A, Yoshida M, Sato M.
- Journal Title
  
  Cell Reprogram
  
  Volume: Vol.12 Pages: 67-74
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580311
[Journal Article] Production of genetically modified porcine blastocysts by somatic cell nuclear transfer : preliminary results to ward production of xenograft-competent miniature pigs2010
- Author(s)
  Himaki T, Watanabe S, Chi H, Yoshida M, Miyoshi K, Sato M
- Journal Title
  
  J Reprod Dev
  
  Volume: 56 Pages: 630-638
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580311
[Journal Article] Usefulness of a non-invasive reporter system for monitoring reprogramming state in pig cells : Results of a cell fusion experiment2010
- Author(s)
  Ozawa A, Akasaka E, Watanabe S, Yoshida M, Miyoshi K, Sato M.
- Journal Title
  
  Journal of Reproduction and Development
  
  Volume: 56巻 Pages: 363-369
- NAID
  10026621377
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Latrunculin a dramatically improves the developmental capacity of nuclear transfer embryos derived from gene-modified clawn miniature pig cells2010
- Author(s)
  Himaki T, Mori H, Mizobe Y, Miyoshi K, Sato M, Takao S, Yoshida M.
- Journal Title
  
  Cell Reprogram
  
  Volume: Vol.12 Pages: 127-131
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580311
[Journal Article] Valproic acid enhances in vitro development and Oct-3/4 expression of miniature pig somatic cell nuclear transfer embryos2010
- Author(s)
  Miyoshi K, et al.
- Journal Title
  
  Cell Reprogram 12
  
  Pages: 67-74
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Latrunculin a dramatically improves the developmental capacity of nuclear transfer embryos derived from gene-modified clawn miniature pig cells2010
- Author(s)
  Himaki T, Mori H, Mizobe Y, Miyoshi K, Sato M, Takao S, Yoshida M
- Journal Title
  
  Cell Reprogram
  
  Volume: 12 Pages: 127-131
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580311
[Journal Article] Effects of trichostatin A on in vitro development and transgene function in somatic cell nuclear transfer embryos derived from transgenic Clawn miniature pig cells2010
- Author(s)
  Himaki T, Yokomine TA, Sato M, Takao S, Miyoshi K, Yoshida M.
- Journal Title
  
  Anim Sci J
  
  Volume: Vol.81
- NAID
  10027889588
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580311
[Journal Article] Enhancement of cytoplasmic maturation of in vitro-matured pig oocytes by mechanical vibration2010
- Author(s)
  Mizobe Y, Yoshida M, Miyoshi K
- Journal Title
  
  Journal of Reproduction and Development
  
  Volume: 56 Pages: 285-290
- NAID
  10026324747
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22580321
[Journal Article] Valpronic acid enhances in vitro development and Oct-3/4 expression of miniature pig somatic cell nuclear transfer embryos2010
- Author(s)
  Miyoshi K, Mori H, Mizobe Y, Akasaka E, Ozawa A, Yoshida M, Sato M.
- Journal Title
  
  Cellular Reprogramming 12
  
  Pages: 67-74
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580311
[Journal Article] Stage-specific effects of osmolarity of a culture medium on development of pig oocytes and miniature pig somatic cell nuclear transfer embryos activated by ultrasound treatment2010
- Author(s)
  Mizobe Y, Kurino S, Sata Y, Mori H, Yoshida M, Miyoshi K
- Journal Title
  
  Animal Science Journal
  
  Volume: 81 Pages: 453-460
- NAID
  10027889195
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22580321
[Journal Article] Production of genetically modified porcine blastocysts by somatic cell nuclear transfer: Preliminary results toward production of xenograft-competent miniature pigs2010
- Author(s)
  Himaki T, WatanabeS, ChiH, YoshidaM, Miyoshi K, Sato M
- Journal Title
  
  Journal of Reproduction and Development
  
  Volume: 56 Pages: 630-638
- NAID
  10027730311
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22580321
[Journal Article] Development of a noninvasive monitoring system for evaluation of Oct-3/4 promoter status in miniature pig somatic cell nuclear transfer embryos2009
- Author(s)
  Miyoshi K, Mori H, Mizobe Y, Akasaka E, Ozawa A, Yoshida M, Sato M
- Journal Title
  
  J Reprod Dev 55
  
  Pages: 661-669
- NAID
  10026322831
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580311
[Journal Article] Development of a noninvasive monitoring system for evaluation of Oct-3/4 promoter status in miniature pig somatic cell nuclear transfer embryos2009
- Author(s)
  Miyoshi K, et al.
- Journal Title
  
  J Reprod Dev 55
  
  Pages: 661-669
- NAID
  10026322831
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Development of a noninvasive monitoring system for evaluation of Oct-3/4 promoter status in miniature pig somatic cell nuclear transfer embryos2009
- Author(s)
  Miyoshi K, Mori H, Mizobe Y, Akasaka E, Ozawa A, Yoshida M, Sato M.
- Journal Title
  
  J Reprod Dev
  
  Volume: Vol.55 Pages: 661-669
- NAID
  10026322831
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580311
[Journal Article] プロスタグランジンF2α類縁物質と性腺刺激ホルモン投与によるクラウン系雌ミニブタの発情同期化に関する研究2009
- Author(s)
  日巻武裕, 梅野知子, 三好和睦, 吉田光敏
- Journal Title
  
  鹿児島県獣医師会会報
  
  Volume: Vol.23 Pages: 5-7
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580311
[Journal Article] Development of a noninvasive monitoring system for evaluation of Oct-3/4 promoter status in miniature pig somatic cell nuclear transfer embryos2009
- Author(s)
  Miyoshi K, Mori H, Mizobe Y, Akasaka E, Ozawa A, Yoshida M, Sato M.
- Journal Title
  
  Journal of Reproduction and Development
  
  Volume: 55巻 Pages: 661-669
- NAID
  10026322831
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Journal Article] Effects of demecolcine and sucrose on the incidence of cytoplasmic protrusions containing chromosomes in pig oocytes matured in vitro2008
- Author(s)
  Miyoshi K, Mori H, Yamamoto H, Kishimoto M, Yoshida M
- Journal Title
  
  Journal of Reproduction and Development 54巻
  
  Pages: 117-121
- NAID
  10021218892
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19580328
[Journal Article] Activation and parthenogenetic development of pig oocytes exposed to ultrasound in media containing different concentrations of Ca2+2008
- Author(s)
  Miyoshi K, Fujimoto Y, Mori H, Yoshida M
- Journal Title
  
  Journal of Reproduction and Development 54巻
  
  Pages: 42-45
- NAID
  10021218462
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19580328
[Journal Article] Effects of demecolcine and sucrose on the incidence of cytoplasmic protrusions containing chromosomes in pig oocytes matured in vitro2008
- Author(s)
  Miyoshi K
- Journal Title
  
  Journal of Reproduction and Development 54
  
  Pages: 117-121
- NAID
  10021218892
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19580328
[Journal Article] Activation and parthenogenetic development of pig oocytes exposed toultrasound in media containing different concentrations of Ca^<2+>2008
- Author(s)
  Miyoshi K
- Journal Title
  
  Journal of Reproduction and Development 54
  
  Pages: 42-45
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19580328
[Journal Article] Effects of demecolcine and sucrose on the incidence of cytoplasmicprotrusions containing chromosomes in pig oocytes matured in vitro.2008
- Author(s)
  Miyoshi K, Mori H, Yamamoto H, Kishimoto M, Yoshida M
- Journal Title
  
  J Reprod Dev 54
  
  Pages: 117-121
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580311
[Journal Article] Effects of cycloheximide on parthenogenetic development of pig oocytes activated by ultrasound treatment2008
- Author(s)
  Mori H, Mizobe Y, Inoue S, Uenohara A, Takeda M, Yoshida M, Miyoshi K
- Journal Title
  
  Journal of Reproduction and Development 54巻
  
  Pages: 364-369
- NAID
  10024412377
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19580328
[Journal Article] Effects of demecolcine and sucrose on the incidence of cytoplasmic protrusions containing chromosomes in pig oocytes matured in vitro2008
- Author(s)
  Miyoshi K
- Journal Title
  
  Journal of Reproduction and Development (印刷中)
- NAID
  10021218892
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19580328
[Journal Article] Effects of cycloheximide on parthenogenetic development of pig oocytesactivated by ultrasound treatment.2008
- Author(s)
  Mori H, Mizobe Y, Inoue S, Uenohara A, Takeda M, Yoshida M, Miyoshi K
- Journal Title
  
  J Reprod Dev 54
  
  Pages: 364-369
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20580311
[Journal Article] Birth of cloned miniature pigs derived from somatic cell nuclear transferred embryos activated by ultrasound treatment2007
- Author(s)
  Miyoshi K, Inoue S, Himaki T, Mikawa S, Yoshida M
- Journal Title
  
  Molecular Reproduction and Development 74巻
  
  Pages: 1568-1574
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19580328
[Journal Article] Birth of cloned miniature pigs derived from somatic cell nuclear transferred embryos activated by ultrasound treatment2007
- Author(s)
  Miyoshi K
- Journal Title
  
  Molecular Reproduction and Development 74
  
  Pages: 1568-1574
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-19580328
[Journal Article] Birth of cloned miniature pigs derived from somatic cell nuclear transferred embryos activated by ultrasound treatment2007
- Author(s)
  Miyoshi K, Inoue S, Himaki T, Mikawa S, Yoshida M
- Journal Title
  
  Molecular Reproduction and Development (印刷中)
- Data Source
  KAKENHI-PROJECT-16688008
[Journal Article] In vitro development of cloned embryos derived from miniature pig somatic cells after activation by ultrasound stimulation2006
- Author(s)
  Miyoshi K, Sato K, Yoshida M
- Journal Title
  
  Cloning and Stem Cells 8
  
  Pages: 159-165
- Data Source
  KAKENHI-PROJECT-16688008
[Journal Article] 超音波を用いたブタ卵子活性化法の開発および体細胞クローンミニブタ作出への応用2006
- Author(s)
  三好和睦
- Journal Title
  
  BIO九州 180
  
  Pages: 1-5
- Data Source
  KAKENHI-PROJECT-16688008
[Journal Article] Utility of ultrasound stimulation for activation of pig oocytes matured in vitro2005
- Author(s)
  Sato K, Yoshida M, Miyoshi K
- Journal Title
  
  Molecular Reproduction and Development 72
  
  Pages: 396-403
- Data Source
  KAKENHI-PROJECT-16688008
[Journal Article] Optimization of calcium concentrations in fusion and activation media for production of cloned embryos from miniature pig somatic cells2005
- Author(s)
  Miyoshi K, Yuki Y, Yoshida M
- Journal Title
  
  Journal of Reproduction and Development 51
  
  Pages: 699-706
- Data Source
  KAKENHI-PROJECT-16688008
[Journal Article] Beneficial effects of reversine on in vitro development of miniaturepig somatic cell nuclear transfer embryos
- Author(s)
  Miyoshi K, et al.
- Journal Title
  
  J Reprod Dev (in press)
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-21580350
[Patent] ブタ生殖細胞の凍結保存液及び凍結保存方法2022
- Inventor(s)
  三好和睦, 井尻萌, 玄丞烋, 玄優基
- Industrial Property Rights Holder
  株式会社バイオベルデ
- Industrial Property Rights Type
  特許
- Industrial Property Number
  2022-082490
- Filing Date
  2022
- Data Source
  KAKENHI-PROJECT-20K06472
[Patent] 超音波による卵子またはクローン胚の活性化方法ならびに該方法により活性化したクローン胚からのクローン動物の作出2011
- Inventor(s)
  三好和睦, 吉田光敏
- Industrial Property Rights Holder
  鹿児島大学
- Filing Date
  2011-07-08
- Data Source
  KAKENHI-PROJECT-22580321
[Patent] 超音波による卵子またはクローン胚の活性化方法ならびに該当方により活性化したクローン胚からのクローン動物の作出2005
- Inventor(s)
  三好和睦, 吉田光敏
- Industrial Property Rights Holder
  国立大学法人鹿児島大学
- Industrial Property Number
  2005-337656
- Filing Date
  2005-11-22
- Data Source
  KAKENHI-PROJECT-16688008
[Presentation] Carboxylated poly-L-lysineおよび3,3-Dimethylglutaric anhydride poly-L-lysineがブタ胚盤胞の耐凍性に及ぼす影響2022
- Author(s)
  Hansol Jin, 井尻萌, 三好和睦
- Organizer
  第15回日本暖地畜産学会
- Data Source
  KAKENHI-PROJECT-20K06472
[Presentation] The world’s smallest Microminipigs and its clone: A novel atherosclerosis-prone miniature pigs2018
- Author(s)
  谷本　昭英、川口　博明、佐藤　正宏、三好　和睦、大竹　正剛、三浦　直樹、野口　倫子
- Organizer
  第19回　Atherosclerosis and Biolipid Conference
- Invited
- Data Source
  KAKENHI-PROJECT-16H05176
[Presentation] 体細胞核移植胚への直接electroporation（GENTEP）は効率的なゲノム編集ブタ作製に有効である2018
- Author(s)
  佐藤正宏, 三好和睦, 松永将伍, 渡部聡, 中村伸吾, 川口博明, 谷本昭英
- Organizer
  第41回日本分子生物学会
- Data Source
  KAKENHI-PROJECT-16K08085
[Presentation] マイクロミニピッグにおける体細胞クローニング2018
- Author(s)
  三好　和睦
- Organizer
  第20回応用薬理シンポジウム
- Invited
- Data Source
  KAKENHI-PROJECT-16H05176
[Presentation] マイクロミニピッグにおける体細胞クローニング技術の確立およびそれを用いた遺伝子ノックアウト動物作出の試み2018
- Author(s)
  三好和睦
- Organizer
  第20回応用薬理シンポジウム
- Invited
- Data Source
  KAKENHI-PROJECT-16K08085
[Presentation] The world’s smallest Microminipigs and its clone2017
- Author(s)
  Akihide Tanimoto, Hiroaki Kawaguchi, Kazuchima Miyoshi, Masayoshi Otake
- Organizer
  7th International Congress on Rabbit Biotechnology
- Invited / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-16H05176
[Presentation] ヒトアポリポプロテイン(a)発現ミニブタの作出2016
- Author(s)
  小沢政之, 大久津昌治, 三好和睦
- Organizer
  第109回日本繁殖生物学会
- Place of Presentation
  麻布大学（神奈川県相模原市）
- Year and Date
  2016-09-12
- Data Source
  KAKENHI-PROJECT-16K08085
[Presentation] マイクロミニピッグを用いた医学研究の現状2016
- Author(s)
  谷本昭英、川口博明、三好和睦、大竹　正剛
- Organizer
  第48回日本動脈硬化学会総会・学術集会
- Invited
- Data Source
  KAKENHI-PROJECT-16H05176
[Presentation] piggyBac遺伝子導入系により複数遺伝子が同時導入されたブタ細胞は核移植後のクローン胚の発生を保証する2016
- Author(s)
  佐藤正宏，前田昂亮，郡山実優，稲田絵美，齋藤一誠，大塚正人，中村伸吾，桜井敬之，渡部聡，三好和睦
- Organizer
  第109回日本繁殖生物学会
- Place of Presentation
  麻布大学（神奈川県相模原市）
- Year and Date
  2016-09-12
- Data Source
  KAKENHI-PROJECT-15K07695
[Presentation] piggyBac遺伝子導入系により複数遺伝子が同時導入されたブタ細胞は核移植後のクローン胚の発生を保証する2016
- Author(s)
  佐藤正宏, 前田昂亮, 郡山実優, 稲田絵美, 齋藤一誠, 大塚正人, 中村伸吾, 桜井敬之, 渡部聡, 三好和睦
- Organizer
  第109回日本繁殖生物学会
- Place of Presentation
  麻布大学（神奈川県相模原市）
- Year and Date
  2016-09-12
- Data Source
  KAKENHI-PROJECT-16K08085
[Presentation] piggyBac遺伝子導入系により複数遺伝子が同時導入されたブタ細胞は核移植後のクローン胚の発生を保証する2016
- Author(s)
  佐藤正宏，前田昂亮，郡山実優，稲田絵美，齋藤一誠，大塚正人，中村伸吾，桜井敬之，渡部聡，三好和睦
- Organizer
  第109回日本繁殖生物学会
- Data Source
  KAKENHI-PROJECT-16H05176
[Presentation] ブタ単為発生卵細胞質へのCRISPR/Cas9系mRNAの直接顕微注入によるゲノム編集は多様な標的遺伝子変異を持つ細胞を生む2015
- Author(s)
  佐藤正宏, 郡山実優, 渡部聡, 大塚正人, 桜井敬之, 稲田絵美, 齋藤一誠, 中村伸吾, 三好和睦
- Organizer
  第108回日本繁殖生物学会大会
- Place of Presentation
  宮崎大学木花キャンパス（宮崎県・宮崎市）
- Year and Date
  2015-09-17
- Data Source
  KAKENHI-PROJECT-15K07695
[Presentation] ブタ単為発生卵細胞質へのCRISPR/Cas9系mRNAの直接顕微注入によるゲノム編集は多様な標的遺伝子変異を持つ細胞を生む2015
- Author(s)
  佐藤正宏, 郡山実優, 渡部聡, 大塚正人, 桜井敬之, 稲田絵美, 齋藤一誠, 中村伸吾, 三好和睦
- Organizer
  第108回日本繁殖生物学会
- Place of Presentation
  宮崎大学（宮崎県宮崎市）
- Year and Date
  2015-09-17
- Data Source
  KAKENHI-PROJECT-25450475
[Presentation] ミニブタにおける体細胞クローニング技術の確立およびそれを用いた新規商品の評価・開発2015
- Author(s)
  三好和睦
- Organizer
  先進医用ミニブタの開発と前臨床研究拠点形成プロジェクト第3回公開シンポジウム
- Place of Presentation
  鹿児島大学(鹿児島県鹿児島市）
- Year and Date
  2015-03-24
- Invited
- Data Source
  KAKENHI-PROJECT-25450475
[Presentation] バルプロ酸処理を用いた体細胞クローンマイクロミニピッグの作出2015
- Author(s)
  前田昂亮, 秋岡幸兵, 野口倫子, 三浦直樹, 川口博明, 谷本昭英, 三好和睦
- Organizer
  第8回日本暖地畜産学会
- Place of Presentation
  東海大学（熊本県熊本市）
- Year and Date
  2015-10-24
- Data Source
  KAKENHI-PROJECT-25450475
[Presentation] 次世代型KOシステムCRISPR/Cas9ゲノム編集法による遺伝子改変ブタ作成への可能性2015
- Author(s)
  佐藤正宏, 郡山実優, 三好和睦
- Organizer
  先進医用ミニブタの開発と前臨床研究拠点形成プロジェクト第3回公開シンポジウム
- Place of Presentation
  鹿児島大学(鹿児島県鹿児島市）
- Year and Date
  2015-03-24
- Invited
- Data Source
  KAKENHI-PROJECT-25450475
[Presentation] トランスポゾンPiggyBacシステムによる複数遺伝子のブタ細胞への同時導入2013
- Author(s)
  郡山実優, 稲田絵美, 齋藤一誠, 三浦浩美, 大塚正人, 中村伸吾, 桜井敬之, 渡部聡, 三好和睦, 佐藤正宏
- Organizer
  第106回日本繁殖生物学会
- Place of Presentation
  東京農工大学府中キャンパス（東京都）
- Data Source
  KAKENHI-PROJECT-25450475
[Presentation] トランスポゾンPiggyBacシステムによる複数遺伝子のブタ細胞への同時導入2013
- Author(s)
  郡山美優、稲田絵美、齋藤一誠、三浦浩美、大塚正人、中村伸吾、桜井敬之、渡部聡、三好和睦、佐藤正宏
- Organizer
  第106回日本繁殖生物学会
- Place of Presentation
  農工大学農学部府中キャンパス（東京都）
- Data Source
  KAKENHI-PROJECT-24580411
[Presentation] RISPR/Cas9による遺伝子編集と標的毒素法との組み合わせは、α-1,3-galactosyltransferase遺伝子を完全にKOしたブタ胎仔性線維芽細胞の効率的作製に有効である2013
- Author(s)
  佐藤正宏、三好和睦、長尾洋三、西洋平、大塚正人、中村伸吾、桜井敬之、渡部聡
- Organizer
  第36回日本分子生物学会年会
- Place of Presentation
  神戸ポートアイランド（神戸市）
- Data Source
  KAKENHI-PROJECT-24580411
[Presentation] CRISPR/Cas9による遺伝子編集と標的毒素法との組み合わせは、α-1,3-galactosyltransferase遺伝子を完全にKOしたブタ胎仔性線維芽細胞の効率的作製に有効である2013
- Author(s)
  佐藤正宏, 三好和睦, 長尾洋三, 西洋平, 大塚正人, 中村伸吾, 桜井敬之, 渡部聡
- Organizer
  第36回日本分子生物学会
- Place of Presentation
  神戸国際展示場（兵庫県）
- Data Source
  KAKENHI-PROJECT-25450475
[Presentation] 振動培養装置がヒト体外受精に及ぼす影響2013
- Author(s)
  溝部大和, 秋吉俊明, 松尾完, 南志穂, 西垣みなみ, 松崎彰子, 福嶋倫子, 山口敦巳, 岡本純英, 三好和睦
- Organizer
  第58回日本生殖医学会
- Place of Presentation
  神戸国際会議場（兵庫県）
- Data Source
  KAKENHI-PROJECT-25450475
[Presentation] ミニブタ体細胞クローン胚作出技術の高度化2012
- Author(s)
  三好和睦
- Organizer
  先進医用ブタの開発と前臨床研究拠点形成プロジェクト第2回公開シンポジウム～ブタの医用動物への展開～
- Place of Presentation
  鹿児島大学稲盛会館(鹿児島県)(招待講演)
- Year and Date
  2012-03-22
- Data Source
  KAKENHI-PROJECT-22580321
[Presentation] ミニブタ体細胞クローン胚作出技術の高度化. 先進医用ブタの開発と前臨床研究拠点形成プロジェクト2012
- Author(s)
  三好和睦
- Organizer
  第2回公開シンポジウム～ブタの医用動物への展開～
- Place of Presentation
  鹿児島大学
- Year and Date
  2012-03-22
- Data Source
  KAKENHI-PROJECT-22580321
[Presentation] 異種移植関連抗原α-Galエピトープ陰性ブタ体細胞の選択的濃縮のためのtargeted toxinの有効性2011
- Author(s)
  池海英、三好和睦、佐藤正宏
- Organizer
  第104回日本繁殖生物学会
- Place of Presentation
  いわて県民情報交流センター・アイーナ(岩手県盛岡市)
- Year and Date
  2011-09-15
- Data Source
  KAKENHI-PROJECT-21580350
[Presentation] 全ゲノム増幅法による核移植ブタ胚でのgene-targeted allele検出の有効性2011
- Author(s)
  池海英、吉田光敏、三好和睦、佐藤正宏
- Organizer
  第58回日本実験動物学会総会
- Place of Presentation
  タワーホール船堀(東京都)
- Data Source
  KAKENHI-PROJECT-21580350
[Presentation] 異種移植関連抗原α-Galエピトープ陰性ブタ体細胞の選択的濃縮のためのtargeted toxinの有効性2011
- Author(s)
  池海英、三好和睦、佐藤正宏
- Organizer
  第104回日本繁殖生物学会
- Place of Presentation
  いわて県民情報交流センター・アイーナ(岩手県)
- Data Source
  KAKENHI-PROJECT-21580350
[Presentation] 活性化後のスクリプタイドまたはスベロイルビスヒドロキサム酸処理がミニブタ体細胞クローン胚の体外発生に及ぼす影響2011
- Author(s)
  小川純輝、小澤政之、三好和睦、吉田光敏
- Organizer
  第104回日本繁殖生物学会
- Place of Presentation
  岩手
- Year and Date
  2011-09-17
- Data Source
  KAKENHI-PROJECT-20580311
[Presentation] 活性化後のラトランキュリンA処理がミニブタ成体腎細胞に由来するクローン胚の発生能に及ぼす影響2010
- Author(s)
  日巻武裕、溝部大和、津田健一郎、末友雅士、三好和睦、吉田光敏
- Organizer
  第112回日本畜産学会
- Place of Presentation
  東京
- Year and Date
  2010-03-28
- Data Source
  KAKENHI-PROJECT-20580311
[Presentation] マイクロアレイを用いたブタ体外成熟卵子の網羅的遺伝子発現解析2010
- Author(s)
  脇詩織、三好和睦、吉田光敏
- Organizer
  第112回日本畜産学会
- Place of Presentation
  東京
- Year and Date
  2010-03-28
- Data Source
  KAKENHI-PROJECT-20580311
[Presentation] 活性化後のラトランキュリンA処理がミニブタ成体腎細胞に由来するクローン胚の発生能に及ぼす影響2010
- Author(s)
  日巻武裕, 溝部大和, 津田健一郎, 末友雅士, 三好和睦, 吉田光敏
- Organizer
  日本畜産学会第112回大会
- Place of Presentation
  明治大学
- Year and Date
  2010-03-28
- Data Source
  KAKENHI-PROJECT-20580311
[Presentation] Latrunculin A dramatically improves the developmental capacity of nuclear transfer embryos derived from gene-modified Clawn miniature pig cells2009
- Author(s)
  Himaki T, Mori H, Mizobe Y, Miyoshi K, Sato M, Takao S, Yoshida M.
- Organizer
  42nd Meeting of the Society for the Study of Reproduction
- Place of Presentation
  アメリカ合衆国
- Year and Date
  2009-07-19
- Data Source
  KAKENHI-PROJECT-20580311
[Presentation] Effect of Latrunculin A on Developmental Potentials of Electrically Activated Porcine Oocvtes2009
- Author(s)
  王建輝, 池海英, 三好和睦, 吉田光敏
- Organizer
  日本畜産学会第110回大会
- Place of Presentation
  日本大学
- Year and Date
  2009-03-29
- Data Source
  KAKENHI-PROJECT-20580311
[Presentation] α-1, 3-galactosiyl-transferase遺伝子ノックアウトベクターを挿入したクラウン系ミニブタ体細胞に由来するクローン胚の体内発生能の検討2009
- Author(s)
  日巻武裕、森寛倫、溝部大和、三好和睦、佐藤正宏、高尾尊身、吉田光敏
- Organizer
  第12回日本異種移植研究会
- Place of Presentation
  鹿児島
- Year and Date
  2009-03-07
- Data Source
  KAKENHI-PROJECT-20580311
[Presentation] バルプロ酸がミニブク体細胞クローン胚の体外発生に及ぼす影響2009
- Author(s)
  森寛倫, 溝部大和, 赤坂恵理, 小澤明央, 吉田光敏, 佐藤正宏, 三好和睦
- Organizer
  第12回日本異種移植研究会
- Place of Presentation
  鹿児島大学
- Year and Date
  2009-03-07
- Data Source
  KAKENHI-PROJECT-20580311
[Presentation] Effect of latrunculin A on developmental potentials of electrically activated porcine oocytes2009
- Author(s)
  王建輝、池海英、三好和睦、吉田光敏
- Organizer
  第110回日本畜産学会
- Place of Presentation
  神奈川
- Year and Date
  2009-03-29
- Data Source
  KAKENHI-PROJECT-20580311
[Presentation] バルプロ酸がミニブタ体細胞クローン胚の体外発生に及ぼす影響2009
- Author(s)
  森寛倫、溝部大和、赤坂恵理、小澤明央、吉田光敏、佐藤正宏、三好和睦
- Organizer
  第12回日本異種植研究会
- Place of Presentation
  鹿児島
- Year and Date
  2009-03-07
- Data Source
  KAKENHI-PROJECT-20580311
[Presentation] 種々のマイクロミニピッグ体細胞を用いて作出したクローン胚の体外発生
- Author(s)
  長尾洋三, 三好和睦
- Organizer
  第107回日本繁殖生物学会
- Place of Presentation
  帯広畜産大学(北海道帯広市）
- Year and Date
  2014-08-21 – 2014-08-23
- Data Source
  KAKENHI-PROJECT-25450475
[Presentation] ブタにおけるCRISPR/Cas9, targeted toxin法を用いたmultiple constructsの標的遺伝子導入システムの開発
- Author(s)
  佐藤正宏、郡山実優、三好和睦、大塚正人、中村伸吾、桜井敬之、渡部聡
- Organizer
  第107回日本繁殖生物学会大会
- Place of Presentation
  帯広畜産大学（北海道・帯広市）
- Year and Date
  2014-08-21 – 2014-08-24
- Data Source
  KAKENHI-PROJECT-24580411
[Presentation] トリス（2-カルボキシエチル）ホスフィン塩酸塩がブタ卵子およびマイクロミニピッグ体細胞クローン胚の体外発生に及ぼす影響
- Author(s)
  前田昂亮, 三好和睦
- Organizer
  第107回日本繁殖生物学会
- Place of Presentation
  帯広畜産大学(北海道帯広市）
- Year and Date
  2014-08-21 – 2014-08-23
- Data Source
  KAKENHI-PROJECT-25450475
[Presentation] Efficient generation of α-1,3-galactosyltransferase-deficient porcine embryonic fibroblasts by CRISPR/Cas9-mediated knock-in system
- Author(s)
  Sato M, Kagoshima A, Saitoh I, Inada E, Miyoshi K, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
- Organizer
  The Asian Federation of Laboratory Animal Science Associations (AFLAS) Congress
- Place of Presentation
  Loyal Chulan Hotel, Kuala Lumpur, Malaysia
- Year and Date
  2014-11-11 – 2014-11-12
- Data Source
  KAKENHI-PROJECT-24580411
[Presentation] ブタにおけるCRISPR/Cas9, targeted toxin法を用いたmultiple constructsの標的遺伝子導入システムの開発
- Author(s)
  佐藤正宏, 郡山実優, 三好和睦, 大塚正人, 中村伸吾, 桜井敬之, 渡部聡
- Organizer
  第107回日本繁殖生物学会
- Place of Presentation
  帯広畜産大学(北海道帯広市）
- Year and Date
  2014-08-21 – 2014-08-23
- Data Source
  KAKENHI-PROJECT-25450475

1. SATO Masahiro (30287099)

# of Collaborated Projects: 11 results

# of Collaborated Products: 67 results
2. KAWAGUCHI HIROAKI (60325777)

# of Collaborated Projects: 5 results

# of Collaborated Products: 7 results
3. YOSHIDA Mitsutoshi (00174954)

# of Collaborated Projects: 3 results

# of Collaborated Products: 46 results
4. Tanimoto Akihide (10217151)

# of Collaborated Projects: 2 results

# of Collaborated Products: 5 results
5. 三浦直樹 (80508036)

# of Collaborated Projects: 2 results

# of Collaborated Products: 2 results
6. KUBOTA CHIKARA (80420652)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
7. 堀内正久 (50264403)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
8. 小澤政之 (90136854)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
9. 岩井聡美 (30566542)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
10. 井尻萌 (20836233)

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
11. HIMAKI Takehiro

# of Collaborated Projects: 1 results

# of Collaborated Products: 11 results
12. MORI Hironori

# of Collaborated Projects: 1 results

# of Collaborated Products: 12 results
13. TSUDA Kennichirou

# of Collaborated Projects: 1 results

# of Collaborated Products: 2 results
14. MIZOBE Yamato

# of Collaborated Projects: 1 results

# of Collaborated Products: 13 results
15. SUETOMO Masashi

# of Collaborated Projects: 1 results

# of Collaborated Products: 2 results
16. OGAWA Jyunki

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
17. AKAHOSHI Kaoru

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
18. NOGUCHI michiko

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
19. OHTAKE masayoshi

# of Collaborated Projects: 1 results

# of Collaborated Products: 2 results
20. NAKAMURA Shingo

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
21. INADA Emi

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
22. KAMIYOSHI Akiko

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
23. MASTUMURA Kazuaki

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
24. 桜井敬之

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
25. 武井則雄

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
26. 大塚正人

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

MIYOSHI Kazuchika 三好 和睦

Research Projects

Research Products

Co-Researchers

Mechanism elucidation of methotrexate arteriosclerosis exacerbation with human atherosclerosis pig model

Principal Investigator

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Review Section

Research Institution

Generation of gene knockout microminipigs based on the modification process to somatic cell nuclear transfer embryosPrincipal Investigator

Principal Investigator

Project Period (FY)

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Review Section

Research Institution

Attempts to perform genome modification towards donor cells for improving SCNT efficiency in pigs

Principal Investigator

Project Period (FY)

Research Category

Review Section

Research Institution

Development of genetically modified microminipigs that can contribute to cancer treatments as disease model animalsPrincipal Investigator

Principal Investigator

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Research Institution

Production of gene-modified pig of next-generation model for Alzheimer's disase

Principal Investigator

Project Period (FY)

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Research Institution

Efficient enrichment of homozygous bi-allelic knockout microminiature porcine cells using a novel selection system and production of genome-edited cloned piglets

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Establishment of somatic cell nuclear transfer techniques in Microminipigs, the smallest pigs in the worldPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Production of cloned genetically modified pigs by a new drug-free gene-modified cell isolation system

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Improvement of somatic cell nuclear transfer techniques in miniature pigs using a noninvasive evaluation system for reprogramming statusPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Development of a general method for obtaining double KO swine cells which will be used as donors for production of cloned homozygous piglets

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Improvement of the developmental capacity of nuclear transfer embryos derived from gene-modified clawn miniature pig cells using a histone deacetylase inhibitor and latrunculin A.

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Development of a noninvasive evaluation system for reprogramming status in miniature pig somatic cell nuclear transfer embryosPrincipal Investigator

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体細胞核移植による効率的なクローンミニブタ作出システムの確立Principal Investigator

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MIYOSHI Kazuchika 三好和睦