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Kumada Yoichi  熊田 陽一

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… Alternative Names

熊田 陽一  クマダ ヨウイチ

KUMADA Youichi  熊田 陽一

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Researcher Number 70452373
Other IDs
Affiliation (Current) 2025: 京都工芸繊維大学, 分子化学系, 教授
Affiliation (based on the past Project Information) *help 2025: 京都工芸繊維大学, 分子化学系, 准教授
2022: 京都工芸繊維大学, 分子化学系, 准教授
2015 – 2020: 京都工芸繊維大学, 分子化学系, 准教授
2013 – 2014: 京都工芸繊維大学, 工芸科学研究科, 准教授
2007 – 2012: Kyoto Institute of Technology, 工芸科学研究科, 助教
2008: 京都工繊大, 助教
Review Section/Research Field
Principal Investigator
Biofunction/Bioprocess / Basic Section 27040:Biofunction and bioprocess engineering-related / Biofunction/Bioprocess
Except Principal Investigator
Biofunction/Bioprocess
Keywords
Principal Investigator
scFv / antibody / 固定化 / 単鎖抗体 / mass transfer / BNPs / Giga pore / affinity chromatography / 高速分離 / タンパク質 … More / クロマトグラフィ分離 / 貫通孔粒子 / dynamic binding capacity / anion exchanger / protein therapeutics / giga pore / anion-exchange / human antibodies / chromatography / Bioprocess / scFv-Fc / Fc Engineering / HF-RabMab / 抗体医薬 / Fc融合タンパク質 / single-chain Fv / 抗体フラグメント / production / CDR-grafting / RabMab / バイオセンサ / リフォールディング / 配向制御 / 材料親和性ペプチド / 窒化ケイ素 / orientation control / immobilization / GST / SiN-tag / SiN / イムノアッセイ / マイクロアレイ / 配向固定 / PMMA-tag / Polystyrene / Pichia pastoris / PS-tag / 医療・福祉 / 分子認識 / バイオデクノロジー / 生物・生体工学 / バイオ関連機器 / バイオテクノロジー / cysteine synthase complex / hydrophilic polystyrene plate / protein immobilization / polystyrene / Polystyrene binding peptide … More
Except Principal Investigator
抗体医薬 / 医療診断 / ペプチド合成 / プロテオーム解析 / アフィニティー濃縮 Less
  • Research Projects

    (8 results)
  • Research Products

    (39 results)
  • Co-Researchers

    (3 People)
  •  バイオ医薬品のオンデマンド精製を実現可能な高耐久性アフィニティリガンドPrincipal Investigator

    • Principal Investigator
      熊田 陽一
    • Project Period (FY)
      2025 – 2027
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Review Section
      Basic Section 27040:Biofunction and bioprocess engineering-related
    • Research Institution
      Kyoto Institute of Technology
  •  Development of a gigaporechromatographic resins that enables rapid and large-scale separation of biopharmaceuticalsPrincipal Investigator

    • Principal Investigator
      Kumada Yoichi
    • Project Period (FY)
      2020 – 2022
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Review Section
      Basic Section 27040:Biofunction and bioprocess engineering-related
    • Research Institution
      Kyoto Institute of Technology
  •  Development of next-generation antibody (HF-RabMab)Principal Investigator

    • Principal Investigator
      Kumada Yoichi
    • Project Period (FY)
      2017 – 2019
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Biofunction/Bioprocess
    • Research Institution
      Kyoto Institute of Technology
  •  Imobilization of scFv through SiN-binding peptide for sensitive RIfS sensorPrincipal Investigator

    • Principal Investigator
      Kumada Yoichi
    • Project Period (FY)
      2014 – 2016
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Biofunction/Bioprocess
    • Research Institution
      Kyoto Institute of Technology
  •  Development of sensitive and inexpensive antibody array chipPrincipal Investigator

    • Principal Investigator
      KUMADA Yoichi
    • Project Period (FY)
      2012 – 2013
    • Research Category
      Grant-in-Aid for Young Scientists (B)
    • Research Field
      Biofunction/Bioprocess
    • Research Institution
      Kyoto Institute of Technology
  •  Fusion of affinity techniques for protein concentration and novel proteome analysis

    • Principal Investigator
      KISHIMOTO Michimasa
    • Project Period (FY)
      2009 – 2011
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Biofunction/Bioprocess
    • Research Institution
      Kyoto Institute of Technology
  •  Development of self-oriented monoclonal antibody for innovative clinical diagnosisPrincipal Investigator

    • Principal Investigator
      KUMADA Youichi
    • Project Period (FY)
      2008 – 2009
    • Research Category
      Grant-in-Aid for Young Scientists (B)
    • Research Field
      Biofunction/Bioprocess
    • Research Institution
      Kyoto Institute of Technology
  •  PS-tagを用いるOne-stepタンパク質間相互作用検出システムの開発Principal Investigator

    • Principal Investigator
      熊田 陽一
    • Project Period (FY)
      2007 – 2008
    • Research Category
      Grant-in-Aid for Young Scientists (Start-up)
    • Research Field
      Biofunction/Bioprocess
    • Research Institution
      Kyoto Institute of Technology

All 2022 2019 2018 2016 2015 2014 2013 2011 2010 2009 2007 Other

All Journal Article Presentation Book Patent

  • [Book] 新生物化学工学2013

    • Author(s)
      岸本通雅、堀内淳一、藤原伸介、熊田陽一
    • Total Pages
      198
    • Publisher
      三共出版
    • Data Source
      KAKENHI-PROJECT-24760649
  • [Journal Article] Control of Accessible Surface Areas and Height Equivalent to a Theoretical Plate using Grafted Dextran during Anion-Exchange Chromatography of Therapeutic Proteins2022

    • Author(s)
      Haingomaholy Michelle Rakotondravao, Ryosuke Takahashi, Takatomo Takai, Yumiko Sakoda, Jun-Ichi Horiuchi, Yoichi Kumada
    • Journal Title

      J. Chem. Eng. Japan / JCEJ

      Volume: 55 Issue: 8 Pages: 267-274

    • DOI

      10.1252/jcej.22we035

    • ISSN
      0021-9592, 1881-1299
    • Year and Date
      2022-08-20
    • Language
      English
    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-20K05228
  • [Journal Article] Strategies for selection and identification of rabbit single-chain Fv antibodies as ligand in affinity chromatographyYoichi Kumada, Haingomaholy Michelle Rakotondravao, Yuya Hasegawa, Yuki Iwashita, Hiromichi Okura, Seiichi Uchimura, Jun-ichi Horiuchi2022

    • Author(s)
      Yoichi Kumada, Haingomaholy Michelle Rakotondravao, Yuya Hasegawa, Yuki Iwashita, Hiromichi Okura, Seiichi Uchimura, Jun-ichi Horiuchi
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 134 Issue: 3 Pages: 233-239

    • DOI

      10.1016/j.jbiosc.2022.06.002

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-20K05228
  • [Journal Article] Identification and characterization of polydimethylsiloxane-binding peptides (PDMS-tag) for oriented immobilization of functional protein on a PDMS surface.2016

    • Author(s)
      Y. Kumada, R. Otsuki, Y. Sakoda, R. Akai, K. Matoba, J. Katayama, M. Kishimoto, J. Horiuchi,
    • Journal Title

      J. Biotechnol.

      Volume: 236 Pages: 193-198

    • DOI

      10.1016/j.jbiotec.2016.08.005

    • Peer Reviewed / Acknowledgement Compliant
    • Data Source
      KAKENHI-PROJECT-26420795
  • [Journal Article] 1.Efficient preparation and site-directed immobilization of VHH antibodies by genetic fusion of poly(methylmethacrylate)-binding peptide (PMMA-tag).2015

    • Author(s)
      Kumada Y, Kang B, Yamakawa K, Kishimoto M, Horiuchi JI
    • Journal Title

      Biotechnology Progress

      Volume: 31 Issue: 6 Pages: 1563-1570

    • DOI

      10.1002/btpr.2169

    • Peer Reviewed / Acknowledgement Compliant
    • Data Source
      KAKENHI-PROJECT-26420795
  • [Journal Article] Immobilization and functional reconstitution of antibody Fab fragment by solid-phase refolding2014

    • Author(s)
      Y. Kumada, K. Hamasaki, A. Nakagawa, E. Sasaki, T. Shirai, M. Okumura, M. Inoue, M. Kishimoto
    • Journal Title

      Journal of Immunological Methods

    • Data Source
      KAKENHI-PROJECT-24760649
  • [Journal Article] Identification and characterization of peptide fragments for the direct and site-specific immobilization of functional proteins onto the surface of silicon nitride2014

    • Author(s)
      Y. Kumada, T. Ootsuka, M. Asada, S. Yoshizuka, M. Chiyama, M. Sakane, F. MD Hasan, K. Sawada, K. Okumura, M. Kishimoto
    • Journal Title

      Journal of Biotechnology

      Volume: in press

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-24760649
  • [Journal Article] Immobilization and functional reconstitution of antibody Fab fragment by solid-phase refolding2013

    • Author(s)
      Yoichi Kumada, Kyoko Hamasaki, Aya Nakagawa, Eiju Sasaki, Tatsinori Shirai, Masahiro Okumura, Manami Inoue, Michimasa Kishimoto
    • Journal Title

      Journal of Immunological Methods

      Volume: 400 - 401 Pages: 70-77

    • DOI

      10.1016/j.jim.2013.10.006

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-24760649
  • [Journal Article] Dynamic analysis of the silver staining gel image of 2-DE for protein quantification2010

    • Author(s)
      M. Kishimoto, Y. Tatsumi, N. Tamesui, Y. Kumada, J. Horiuchi, K. Okumura
    • Journal Title

      Journal of Electrophoresis

      Volume: Vol.51 Pages: 1-7

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21560810
  • [Journal Article] Direct Immobilization of Functional Single-Chain Variable Fragment Antibodies (scFvs) onto a Polystyrene Plate by Genetic Fusion of a Polystyrene-Binding Peptide (PS-tag)2009

    • Author(s)
      Y.Kumada, K.Hamasaki, Y.Shiritani, A.Nakagawa, D.Kuroki, T.Ohse, D.H.Choi, Y.Katakura, M.Kishimoto
    • Journal Title

      Analytical and Bioanalytical Chemistry 395

      Pages: 759-765

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-20760537
  • [Journal Article] Direct Immobilization of Functional Single-Chain Variable Fragment Antibodies (scFvs) onto a Polystyrene Plate by Genetic Fusion of a Polystyrene-Binding Peptide (PS-tag)2009

    • Author(s)
      Y. Kumada, K. Hamasaki, Y. Shiritani, A. Nakagawa, D. Kuroki, T. Ohse, D.H. Choi, Y. Katakura, M. Kishimoto
    • Journal Title

      Analytical and Bioanalytical Chemistry vol.395

      Pages: 759-765

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-20760537
  • [Journal Article] High biological activity of a recombinant protein immobilized onto polystyrene2009

    • Author(s)
      Y.Kumada, Y.Shiritani, K.Hamasaki, T.Ohse, M.Kisihmoto
    • Journal Title

      Biotechnology Journal 4

      Pages: 1190-1197

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-20760537
  • [Journal Article] High biological activity of a recombinant protein immobilized onto polystyrene2009

    • Author(s)
      Y. Kumada, Y. Shiritani, K. Hamasaki, T. Ohse, M. Kisihmoto
    • Journal Title

      Biotechnology Journal vol.4

      Pages: 1190-1197

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-20760537
  • [Journal Article] Characteristics of a liposome immunoassay on a poly (methylmethacrylate) surface2007

    • Author(s)
      S. Y. Hwang, Y. Kumada, G. H. Seong, J. B. Choo, S. Katoh, E. K. Lee
    • Journal Title

      Analytical and Bioanalytical Chemistry 389

      Pages: 2251-2257

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19860042
  • [Journal Article] Protein-protein interaction analysis using an affinity p eptide tag and hydrophilic polystyrene plate2007

    • Author(s)
      Y. Kumada, C. Zhao, R. Ishimura, H. Imanaka, K. Imamura, K. Nakanishi
    • Journal Title

      Journal of Biotechnology 128

      Pages: 354-361

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-19860042
  • [Journal Article] Efficient immobilization of a ligand antibody with high antigen-binding activity by use of a polystyrene binding peptide and an intelligent microtiter plate

    • Author(s)
      Y. Kumada, K. Hamasaki, Y. Shiritani, T. Ohse, M. Kisihmoto
    • Journal Title

      Journal of Biotechnology vol.142

      Pages: 135-141

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-20760537
  • [Journal Article] Identification and characterization of peptide fragments for the direct and site-specific immobilization of functional proteins onto the surface of silicon nitride

    • Author(s)
      Y. Kumada, T. Ootsuka, M. Asada, S. Yoshizuka, M. Chiyama, M. Sakane, F. MD Hasan, K. Sawada, K. Okumura, M. Kishimoto
    • Journal Title

      Journal of Biotechnology

      Volume: (in press)

    • Data Source
      KAKENHI-PROJECT-24760649
  • [Journal Article] High biological activity of a recombinant protein immobilized onto polystyrene

    • Author(s)
      熊田陽一、他4名
    • Journal Title

      Biotechnology Journal (印刷中)

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-20760537
  • [Journal Article] Efficient immobilization of a ligand antibody with high antigen-binding activity by use of a polystyrene binding peptide and an intelligent microtiter plate

    • Author(s)
      熊田陽一、他4名
    • Journal Title

      Journal of Biotechnology (印刷中)

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-20760537
  • [Journal Article] Screening of PC and PMMA-binding peptides for site-specific immobilization of protein

    • Author(s)
      Y. Kumada, S. Murata, Y. Ishikawa, K. Nakatsuka, M. Kishimoto
    • Journal Title

      Journal of Biotechnology

      Volume: (in press)

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21560810
  • [Patent] 抗体のリフォールディング方法、リフォールディングされた抗体の製造方法、リフォールディングされた抗体、及びこれらの利用2013

    • Inventor(s)
      熊田陽一、岸本通雅、石川泰行、藤原勇佐
    • Industrial Property Rights Holder
      京都工芸繊維大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-027862
    • Filing Date
      2013-02-15
    • Data Source
      KAKENHI-PROJECT-24760649
  • [Patent] ポリカーボネート及び/またはポリメタックリル酸メチル親和性ぺプチド、およびその利用2010

    • Inventor(s)
      熊田陽一、岸本通雅、中塚一喜、村田祥
    • Industrial Property Rights Holder
      京都工芸繊維大学
    • Industrial Property Number
      2010-003166
    • Filing Date
      2010-02-16
    • Data Source
      KAKENHI-PROJECT-21560810
  • [Presentation] Super-Secretory Expression of Recombinant Antibody Fragments by Fed-Batch Culture with PID-Control Feeding System2019

    • Author(s)
      Yoichi Kumada, Jun-chi Horiuchi
    • Organizer
      PEPTALK 2019
    • Data Source
      KAKENHI-PROJECT-17K06923
  • [Presentation] エクソソームのアフィニティ分離が可能な単鎖抗体固定化担体の開発2018

    • Author(s)
      小林 巧 , 新 裕太 , 片山 淳子 , 的場 一隆 , 堀内 淳一 , 熊田 陽一
    • Organizer
      日本生物工学会
    • Data Source
      KAKENHI-PROJECT-17K06923
  • [Presentation] 組換え大腸菌を用いた活性型一本鎖抗体の高効率分泌生産2015

    • Author(s)
      李歓・岸本通雅・堀内淳一・熊田陽一
    • Organizer
      化学工学会 秋季大会
    • Place of Presentation
      北海道・札幌市
    • Year and Date
      2015-09-09
    • Data Source
      KAKENHI-PROJECT-26420795
  • [Presentation] プロテオーム解析をもちいた半導体センサ親和性ペプチドのスクリーニング2011

    • Author(s)
      大塚武、寺田貴裕、岸本通雅、熊田陽一
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-27
    • Data Source
      KAKENHI-PROJECT-21560810
  • [Presentation] Analysis of Fermentation Process for Scfv by Proteomics2011

    • Author(s)
      Y.Fujiki, Y.Kumada, M.Kishimoto
    • Organizer
      2011 AIChE(米国化学工学会)Annual Meeting
    • Place of Presentation
      Convention center,ミネアポリスUSA
    • Year and Date
      2011-10-19
    • Data Source
      KAKENHI-PROJECT-21560810
  • [Presentation] プロテオーム解析による抗体生産培養の検証2011

    • Author(s)
      岸本通雅、藤木裕也、熊田陽一、堀内淳一
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-16
    • Data Source
      KAKENHI-PROJECT-21560810
  • [Presentation] Analysis of Fermentation Process for Scfv Production by Proteomics2011

    • Author(s)
      Y. Fujiki, Y. Kumada, M. Kishimoto
    • Organizer
      AIChE Annual Meeting Minnneapolis in USA
    • Year and Date
      2011-10-19
    • Data Source
      KAKENHI-PROJECT-21560810
  • [Presentation] タンパク質固定化用プラスチック親和性ペプチドの評価2011

    • Author(s)
      石川泰行、村田祥、熊田陽一、岸本通雅
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-27
    • Data Source
      KAKENHI-PROJECT-21560810
  • [Presentation] プロテオーム解析を用いた半導体センサー親和性ペプチドのスクリーニング2011

    • Author(s)
      大塚武、寺田貴裕、岸本通雅、熊田陽一
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-27
    • Data Source
      KAKENHI-PROJECT-21560810
  • [Presentation] ポリスチレン親和性ペプチドを利用したタンパク質の部位特異的固定化と配向制御2009

    • Author(s)
      熊田陽一
    • Organizer
      バイオマテリアル学会年次大会(招待講演)
    • Place of Presentation
      京都
    • Year and Date
      2009-11-17
    • Data Source
      KAKENHI-PROJECT-20760537
  • [Presentation] Novel Single-Chain FIT Antibody-Immobilized Polystyrene Supports: Its Potential and Possibility for Antibody Microarray2009

    • Author(s)
      Y. Kumada, T. Ohse, M. Kishimoto
    • Organizer
      International Congress of Antibody
    • Place of Presentation
      北京(中国)
    • Year and Date
      2009-05-23
    • Data Source
      KAKENHI-PROJECT-20760537
  • [Presentation] Novel polystyrene binding peptide (PS-tag) for site-specific immobilization and orientation control of recombinant antibody fragments2009

    • Author(s)
      Y. Kumada, T. ohse, M. Kishimoto
    • Organizer
      APBioChEC 2009
    • Place of Presentation
      神戸
    • Year and Date
      2009-12-01
    • Data Source
      KAKENHI-PROJECT-20760537
  • [Presentation] Development of Rapid and Sensitive ELISA Systems by Use of Polystyrene Binding Peptides(PS-tags)and Intelligent Microtiter well plate2009

    • Author(s)
      熊田陽一、他2人
    • Organizer
      Biomarker Assay Development
    • Place of Presentation
      米国, サンディエゴ
    • Year and Date
      2009-01-26
    • Data Source
      KAKENHI-PROJECT-20760537
  • [Presentation] Novel Single-Chain Fv Antibody-Immobilized Polystyrene Supports : Its Potential and Possibility for Antibody Microarray2009

    • Author(s)
      Y.Kumada, T.Ohse, M.Kishimoto
    • Organizer
      International Congress of Antibody
    • Place of Presentation
      北京(中国)
    • Year and Date
      2009-05-23
    • Data Source
      KAKENHI-PROJECT-20760537
  • [Presentation] Development of Rapid and Sensitive ELISA Systems by Use of Polystyrene Binding Peptides (PS-tags) and Intelligent Microtiter well plate2009

    • Author(s)
      Y. Kumada, T. Ohse, M. Kishimoto
    • Organizer
      Biomarker Assay Development
    • Place of Presentation
      San Diego (USA)
    • Year and Date
      2009-01-26
    • Data Source
      KAKENHI-PROJECT-20760537
  • [Presentation] ポリスチレン基盤への低分子抗体の部位特異的固定化と活性発現2009

    • Author(s)
      熊田陽一
    • Organizer
      化学工学会秋季大会
    • Place of Presentation
      広島大学
    • Year and Date
      2009-09-16
    • Data Source
      KAKENHI-PROJECT-21560810
  • [Presentation] ポリスチレン基盤への低分子抗体の部位特異的固定化と活性発現2009

    • Author(s)
      熊田陽一, 大瀬琢人, 岸本通雅
    • Organizer
      化学工学会秋季大会
    • Place of Presentation
      広島
    • Year and Date
      2009-09-16
    • Data Source
      KAKENHI-PROJECT-20760537
  • 1.  KISHIMOTO Michimasa (00144436)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 8 results
  • 2.  NOMURA Akinari (20402901)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 3.  IWATA Hiroo (30160120)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results

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