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Sakaguchi Masayoshi  坂口 政吉

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SAKAGUCHI Masayoshi  坂口 政吉

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Researcher Number 80281351
Other IDs
Affiliation (Current) 2025: 工学院大学, 先進工学部, 准教授
Affiliation (based on the past Project Information) *help 2017 – 2019: 工学院大学, 先進工学部, 准教授
2004 – 2005: 工学院大学, 工学部, 助手
1999 – 2001: 工学院大学, 工学部, 助手
1996: Kogakuin University, Department of Applied Chemistry Assistant, 工学部, 助手
Review Section/Research Field
Principal Investigator
Applied microbiology
Except Principal Investigator
応用微生物学・応用生物化学 / Structural biochemistry / Structural biochemistry
Keywords
Principal Investigator
GH15ファミリー酵素 / 古細菌トレハラーゼ / 基質認識 / GH15ファミリー / 糖質分解酵素 / トレハロース / GH15糖質分解酵素 / 古細菌 / トレハラーゼ
Except Principal Investigator
CMP-sialic acid … More / UDP-galactose / Nucleotide sugar transporter / CMP-シアル酸 / 複合糖質 / 糖ヌクレオチド輸送体 / ATP / NAD^+ / バイオリアクター / Golgi apparatus / Glycoconjugate / 形態形成異常 / UDP-ガラクトース / ゴルジ装置 / Bifidobacterium / site-directed mutagenesis / active site / substrate specificity / organic synthesis by enzyme / L-lactate dehydrogenase / タンパク質分解酵素 / ビスィズス菌 / 部位特異的変異 / 活性部位 / 基質特異性 / 酵素による有機合成 / L-乳酸脱水素酵素 / UDP-N-acetylglucosamine / Glycoconjugates / Colon cancer / Glycobiology / CMP-シアル酸輸送体 / ヒスチジンータグ / 可溶化再構成 / UDP-ガラクトース輸送体遺伝子 / UDP-ガラクトース輸送体 / 多重特異的糖ヌクレオチド輸送体 / UST74C輸送体 / UGTre17輸送体 / UDP-N-アセチルグルコサミン / 大腸がん / 糖鎮生物学 / UDP-ガラストーク / COENZYMES / IMMOBILIZED ENZYMES / BIOREACTOR / NAD / バイオイアクター / 補酵素 / 固定化酵素 Less
  • Research Projects

    (5 results)
  • Research Products

    (8 results)
  • Co-Researchers

    (7 People)
  •  Functional analysis of archaeal trehalases and attempt of their properties for novel oligosaccharides productionPrincipal Investigator

    • Principal Investigator
      Sakaguchi Masayoshi
    • Project Period (FY)
      2017 – 2019
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Applied microbiology
    • Research Institution
      Kogakuin University
  •  Substrate recognition and subcellular localization of nucleotide sugar transporters

    • Principal Investigator
      KAWAKITA Masao
    • Project Period (FY)
      2004 – 2005
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Structural biochemistry
    • Research Institution
      KOGAKUIN UNIVERSITY
  •  Nucleotide sugar transporters: Structure and function, and physiological regulation

    • Principal Investigator
      KAWAKITA Masao
    • Project Period (FY)
      1999 – 2001
    • Research Category
      Grant-in-Aid for Scientific Research (B)
    • Research Field
      Structural biochemistry
    • Research Institution
      Kogakuin University
      Tokyo Metropolitan Organization for Medical Research
  •  Synthesis of organic compounds by the enzyme with modified substrate specificity

    • Principal Investigator
      OHTA Takahisa
    • Project Period (FY)
      1999 – 2000
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      応用微生物学・応用生物化学
    • Research Institution
      Kogakuin University
  •  Development of Multi-enzyme bioreactors using coenzyme-collecting system.

    • Principal Investigator
      OHTA Takahisa
    • Project Period (FY)
      1995 – 1996
    • Research Category
      Grant-in-Aid for Scientific Research (B)
    • Research Field
      応用微生物学・応用生物化学
    • Research Institution
      Kogakuin University

All 2020 2019 2018 2017

All Journal Article Presentation

  • [Journal Article] Diverse and common features of trehalases and their contributions to microbial trehalose metabolism2020

    • Author(s)
      Masayoshi Sakaguchi
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 104 Issue: 5 Pages: 1837-1847

    • DOI

      10.1007/s00253-019-10339-7

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-17K07729
  • [Journal Article] The Listeria innocua chitinase LinChi78 has a unique region that is necessary for hydrolytic activity2019

    • Author(s)
      Honda Shotaro、Kimura Masahiro、Wakita Satoshi、Oka Yuji、Kawakita Masao、Oyama Fumitaka、Sakaguchi Masayoshi
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 103 Issue: 4 Pages: 1777-1787

    • DOI

      10.1007/s00253-018-9573-5

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-17K07729
  • [Journal Article] Two trehalose-hydrolyzing enzymes from Crenarchaeon Sulfolobus acidocaldarius exhibit distinct activities and affinities toward trehalose2018

    • Author(s)
      Yuasa Mitsuhiro、Okamura Takeshi、Kimura Masahiro、Honda Shotaro、Shin Yongchol、Kawakita Masao、Oyama Fumitaka、Sakaguchi Masayoshi
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 102 Issue: 10 Pages: 4445-4455

    • DOI

      10.1007/s00253-018-8915-7

    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-17K07729
  • [Presentation] GH15古細菌トレハラーゼの機能解析2019

    • Author(s)
      坂口 政吉、湯浅 充洋、多田 凌吾、南澤 秋弥、甲田 有梨奈、川喜田 正夫
    • Organizer
      日本農芸化学会2020年度大会
    • Data Source
      KAKENHI-PROJECT-17K07729
  • [Presentation] リステリアキチナーゼの機能未知領域の機能2019

    • Author(s)
      坂口政吉,本田翔太郎,木村将大,脇田悟誌,岡侑司,川喜田正夫
    • Organizer
      日本農芸化学会2019年度(H31年度)大会[東京]
    • Data Source
      KAKENHI-PROJECT-17K07729
  • [Presentation] 古細菌トレハラーゼのN末端領域の機能2018

    • Author(s)
      ○坂口政吉,久米杏奈,湯浅充洋,本田翔太郎,小山文隆,川喜田正夫
    • Organizer
      日本農芸化学会2018年度大会 [名古屋]
    • Data Source
      KAKENHI-PROJECT-17K07729
  • [Presentation] 新規古細菌トレハラーゼの大腸菌組換えタンパク質の発現及び性質解析2017

    • Author(s)
      ○湯浅充洋,岡村武司,本田翔太郎,川喜田正夫,小山文隆,坂口政吉
    • Organizer
      日本応用糖質科学会 平成29年度大会(第66回)
    • Data Source
      KAKENHI-PROJECT-17K07729
  • [Presentation] 古細菌トレハラーゼのN末端領域の役割2017

    • Author(s)
      ○坂口政吉,久米杏奈,湯浅充洋,本田翔太郎,小山文隆,川喜田正夫
    • Organizer
      日本応用糖質科学会 平成29年度大会(第66回)
    • Data Source
      KAKENHI-PROJECT-17K07729
  • 1.  OHTA Takahisa (30011844)
    # of Collaborated Projects: 2 results
    # of Collaborated Products: 0 results
  • 2.  KAWAKITA Masao (00012740)
    # of Collaborated Projects: 2 results
    # of Collaborated Products: 0 results
  • 3.  MIKI Toshiaki (10239204)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 4.  AOKI Kazuhisa (00280785)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 5.  ISHIDA Nobuhiro (20291148)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 6.  SUGAHARA Yasusato (20133324)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results
  • 7.  OHKATSU Yasukazu (20011009)
    # of Collaborated Projects: 1 results
    # of Collaborated Products: 0 results

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