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SHIMAZAKI Yoji  島崎 洋次

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Shimazaki Yoji  島崎 洋次

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Researcher Number 80284389
Other IDs
External Links
Affiliation (Current) 2025: 愛媛大学, 理工学研究科(理学系), 准教授
Affiliation (based on the past Project Information) *help 2021 – 2024: 愛媛大学, 理工学研究科(理学系), 准教授
2013 – 2015: 愛媛大学, 理工学研究科, 准教授
2007: Ehime University, Faculty of Science, Ass. Professor
2006: 愛媛大学, 理工学研究科, 助手
2002 – 2005: 愛媛大学, 理学部, 助手
1997 – 2000: 愛媛大学, 理学部, 助手
Review Section/Research Field
Principal Investigator
Basic Section 34020:Analytical chemistry-related / Analytical chemistry / 分離・精製・検出法
Except Principal Investigator
分離・精製・検出法 / Analytical chemistry
Keywords
Principal Investigator
タンパク質 / 電気泳動法 / 酵素活性 / 電気泳動 / 機能と構造 / 糖鎖修飾 / 分離分析法 / 天然超分子 / 酵素 / 天然酵素 … More / タンパク質複合体 / 機能制御 / 非変性 / 分離と溶出 / 超分子 / 捕獲システム / タンパク質凝集体 / 実試料分析 / 蛍光検出 / プロテアーゼ / 生体試料分析 / 微量分析 / アミロイドβ / 抗体固定化膜 / 分離分析 / 一斉分析 / 質量分析 … More
Except Principal Investigator
血漿タンパク質 / MALDI-TOF MS / 2次元ゲル電気泳動 / mass spectrometry / MS / ESI-MS / 質量分析 / two-dimensional electrophoresis / 2次元電気泳動 / isoelectric focusing / plasma proteins / SDS電気泳動 / 等電点電気泳動 / protein interaction / non-denaturing / マイクロ / タンパク質相互作用 / マイクロゲル / 非変性条件 / ヒト血漿 / 微量タンパク質 / SDS electrophoresis / capillary electrophoresis / 血漿 / タンパク質 / 高分離能分析 / キャピラリー電気泳動 / peptide mass fumernrintine / plasma protein / micro 2-DE / protein complex / MALDI-TOF-MS / ペプチドマスフィンガープリンティング / マイクロ2次元電気泳動 / 非変性 / タンパク質複合体 / micro gel / two-dimensional gel electrophoresis / human plasma / 生理的条件 / MALDI-TOP MS / minor proteins / SDS-gel electrophoresis / 構造解析 / 分離 / 微量 / 電気泳動 / MALDI-TOFMS / high-performance analysis / separation / minor protein components / human plasma proteins / 高性能分取 / 高性能分離 / two-demensional gel electrophoresis / high-resolution anaiysis / high-sensitivity analysis / myeloma / 血清 / 免疫グロブリン / 高感度分析 / 骨髄腫 Less
  • Research Projects

    (9 results)
  • Research Products

    (40 results)
  • Co-Researchers

    (3 People)
  •  タンパク質の機能・構造とその糖鎖修飾の状態との関係を分析する新規電気泳動法の構築Principal Investigator

    • Principal Investigator
      島崎 洋次
    • Project Period (FY)
      2024 – 2026
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Review Section
      Basic Section 34020:Analytical chemistry-related
    • Research Institution
      Ehime University
  •  Construction of high resolution separation analysis and function control methods for natural supramoleculesPrincipal Investigator

    • Principal Investigator
      島崎 洋次
    • Project Period (FY)
      2021 – 2024
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Review Section
      Basic Section 34020:Analytical chemistry-related
    • Research Institution
      Ehime University
  •  Construction of analytical systems in fibrillation of target proteinPrincipal Investigator

    • Principal Investigator
      Shimazaki Yoji
    • Project Period (FY)
      2013 – 2015
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Analytical chemistry
    • Research Institution
      Ehime University
  •  Construction of a high-performance system to collect protein information by the combination of non-denaturing two-dimensional electrophoresis and mass spectrometry

    • Principal Investigator
      MANABE Takashi
    • Project Period (FY)
      2006 – 2007
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Analytical chemistry
    • Research Institution
      Ehime University
  •  Analysis of protein-protein interactions in human plasma using non-denaturing two-dimensional electrophoresis and mass spectrometry

    • Principal Investigator
      MANABE Takashi
    • Project Period (FY)
      2004 – 2005
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      Analytical chemistry
    • Research Institution
      Ehime University
  •  電気泳動法による水溶性酵素の機能構造一斉分析法の開発Principal Investigator

    • Principal Investigator
      島崎 洋次
    • Project Period (FY)
      2002 – 2003
    • Research Category
      Grant-in-Aid for Young Scientists (B)
    • Research Field
      分離・精製・検出法
    • Research Institution
      Ehime University
  •  Separation and structural analysis of IgG-binding minor proteins in human plasma

    • Principal Investigator
      MANABE Takashi
    • Project Period (FY)
      2002 – 2003
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      分離・精製・検出法
    • Research Institution
      Ehime University
  •  Development of high-performance techniques for the separation and analysis of minor protein components in human plasma

    • Principal Investigator
      MANABE Takashi
    • Project Period (FY)
      1999 – 2000
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      分離・精製・検出法
    • Research Institution
      Ehime University
  •  Develepment of high sensitivity and high resolution techniques for the analysis of myeloma proteins

    • Principal Investigator
      MANABE Takashi
    • Project Period (FY)
      1997 – 1998
    • Research Category
      Grant-in-Aid for Scientific Research (C)
    • Research Field
      分離・精製・検出法
    • Research Institution
      Ehime University

All 2024 2023 2022 2021 2016 2015 2014 2013 2006 2005 2004 Other

All Journal Article Presentation

  • [Journal Article] 第62回日本電気泳動学会学会賞(児玉賞)受賞者論文:非変性条件の2次元電気泳動による各種酵素およびその複合体の分離と機能解析2024

    • Author(s)
      Shimazaki Youji
    • Journal Title

      Electrophoresis Letters

      Volume: 68

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21K05128
  • [Journal Article] Prospects and challenges of native protein separation and analysis using non-denaturing two-dimensional gel electrophoresis2023

    • Author(s)
      Shimazaki Youji、Fuke Urara、Tokuda Misaki
    • Journal Title

      Electrophoresis Letters

      Volume: 67 Issue: 2 Pages: 71-75

    • DOI

      10.2198/electroph.67.71

    • ISSN
      2189-2628, 2189-2636
    • Language
      Japanese
    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-21K05128
  • [Journal Article] Analysis of the enzymatic degradation of lysozyme fibrils using a combination method of non-denaturing gel electrophoresis and double staining with Coomassie brilliant blue G-250 and R-250 dyes2023

    • Author(s)
      Kenichiro Nagata, Ryo Ashikaga, Wakako Mori, Tamotsu Zako, Youji Shimazaki
    • Journal Title

      Analytical Sciences

      Volume: 39 Pages: 267-274

    • DOI

      10.1007/s44211-022-00229-w

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21K05128, KAKENHI-PROJECT-22K19114, KAKENHI-PROJECT-19H02527
  • [Journal Article] Applicability of esterase activity change analysis using a combined method of non-denaturing two-dimensional electrophoresis and reverse staining2023

    • Author(s)
      Inoue Maho、Shimazaki Youji
    • Journal Title

      J. Electrophor

      Volume: 67 Issue: 1 Pages: 1-7

    • DOI

      10.2198/jelectroph.67.1

    • ISSN
      1349-9394, 1349-9408
    • Language
      Japanese
    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-21K05128
  • [Journal Article] Electrophoretic extraction of the carboxylesterase-transferrin complexes after separation and detection by non-denaturing two-dimensional electrophoresis2023

    • Author(s)
      Shimazaki Youji、Nakao Karin、Fuke Urara
    • Journal Title

      Electrophoresis Letters

      Volume: 67 Issue: 1 Pages: 37-42

    • DOI

      10.2198/electroph.67.37

    • ISSN
      2189-2628, 2189-2636
    • Language
      Japanese
    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-21K05128
  • [Journal Article] Electrophoretic extraction of protein complexes after separation and detection by a combined method of non-denaturing two-dimensional electrophoresis and reversible staining2022

    • Author(s)
      Karin Nakao, Youji Shimazaki
    • Journal Title

      J. Electrophor

      Volume: 66 Issue: 1 Pages: 5-11

    • DOI

      10.2198/jelectroph.66.5

    • ISSN
      1349-9394, 1349-9408
    • Language
      Japanese
    • Peer Reviewed / Open Access
    • Data Source
      KAKENHI-PROJECT-21K05128
  • [Journal Article] Characterization of enzymatic activity of lysozyme in lysozyme-ovotransferrin complex before and after treatment with trypsin2021

    • Author(s)
      Youji Shimazaki, Shunta Yabu
    • Journal Title

      Separation Science Plus

      Volume: 4 Pages: 377-383

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-21K05128
  • [Journal Article] Retaining activity of enzymes after capture and extraction within a single-drop of biological fluid using immunoaffinity membranes,2016

    • Author(s)
      Youji Shimazaki ,Yuki Sato
    • Journal Title

      Journal of Chromatography B

      Volume: in press

    • Peer Reviewed / Acknowledgement Compliant
    • Data Source
      KAKENHI-PROJECT-25410145
  • [Journal Article] Combined method of immunoaffinity membrane within tubes and MALDI-TOF MS for capturing and analyzing amyloid beta,2015

    • Author(s)
      Youji Shimazaki, Yoko Takatsu
    • Journal Title

      Applied Biochemistry and Biotechnology

      Volume: 177 Issue: 7 Pages: 1565-1571

    • DOI

      10.1007/s12010-015-1837-2

    • Peer Reviewed / Acknowledgement Compliant
    • Data Source
      KAKENHI-PROJECT-25410145
  • [Journal Article] A microfluidic device containing membrane-immobilized antibodies for successively capturing cytosolic enzymes2014

    • Author(s)
      Youji Shimazaki, Ai. Hashimoto
    • Journal Title

      Talanta

      Volume: 125 Pages: 400-404

    • DOI

      10.1016/j.talanta.2014.03.001

    • Peer Reviewed / Acknowledgement Compliant
    • Data Source
      KAKENHI-PROJECT-25410145
  • [Journal Article] Micro-scale extraction and analysis of intact carboxylesterase after trapping on an immunoaffinity membrane surface2014

    • Author(s)
      Ayaka Kimura, Youji Shimazaki
    • Journal Title

      Applied Biochemistry and Biotechnology

      Volume: 172 Issue: 8 Pages: 4053-4061

    • DOI

      10.1007/s12010-014-0807-4

    • Peer Reviewed / Acknowledgement Compliant
    • Data Source
      KAKENHI-PROJECT-25410145
  • [Journal Article] A microfluidic device containing membrane-immobilized antibodies for successively capturing cytosolic enzymes2014

    • Author(s)
      Youji Shimazaki, Ai Hashimoto
    • Journal Title

      Talanta

      Volume: in press

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-25410145
  • [Journal Article] Isolation and analysis of amyloid -beta 1-42 monomer and oligomers in liquid droplets using an immunoaffinity membrane,2014

    • Author(s)
      Youji Shimazaki, Yu Hirose
    • Journal Title

      Journal of Chromatography B

      Volume: 972 Pages: 53-57

    • DOI

      10.1016/j.jchromb.2014.09.021

    • Peer Reviewed / Acknowledgement Compliant
    • Data Source
      KAKENHI-PROJECT-25410145
  • [Journal Article] Antigen digestion on the target plate of MALDI-TOF MS after isolation using an immunoaffinity membrane2013

    • Author(s)
      Youji Shimazaki, Yuri Nishimura, Masaki Saito
    • Journal Title

      Journal of Phamaceutical and Biomedical Analysis

      Volume: 83 Pages: 293-298

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-25410145
  • [Journal Article] Analysis of trypsin inhibition activity in human plasma proteins after separation by non-denaturing two-dimensional electrophoresis2013

    • Author(s)
      Youji Shimazaki, Madoka Michhiro
    • Journal Title

      Clinica Chimica Acta

      Volume: 425 Pages: 48-53

    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-25410145
  • [Journal Article] Analysis of hydrolytic activity of phospholipase Calfa from porcine retina on retinyl ester and phosphatidylcholine using non-denaturing two-dimensional electrophoresis and mass spectrometry2006

    • Author(s)
      島崎 洋次
    • Journal Title

      J. Chromatogr. B. 843

      Pages: 42-46

    • Data Source
      KAKENHI-PROJECT-18550076
  • [Journal Article] Analysis of hydrolytic activity of phospholipase Calfa from porcine retina on retinyl ester and phosphatidyl choline using non-denaturing two-dimensional electrophoresis and mass spectrometry2006

    • Author(s)
      島崎 洋次
    • Journal Title

      J. Chromatogr. B. 843

      Pages: 42-46

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
    • Data Source
      KAKENHI-PROJECT-18550076
  • [Journal Article] Analysis of hydrolytic activity of phospholipase Calfa from porcine retina on retinyl ester and phosphatidyl choline using non-denaturing two-dimensional electrophoresis and mass spectrometry2006

    • Author(s)
      Shimazaki, Y., Kishi, IL, Mori, M., Yasuda, C., Manabe, T
    • Journal Title

      J. Chromatogr B 843

      Pages: 42-46

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-18550076
  • [Journal Article] Detection of activity and mass spectrometric identification of mouse liver carboxylesterase and aldehyde dehydrogenase separated by non-denaturing two-dimensional electrophoresis after extraction with detergents2005

    • Author(s)
      島崎 洋次, 真鍋 敬
    • Journal Title

      Biochim.Biophys.Acta 1749・1

      Pages: 95-101

    • Data Source
      KAKENHI-PROJECT-16550077
  • [Journal Article] Detection of activity and mass spectrometric identification of mouse liver caboxylesterase and aldehyde dehydrogenase separated by non-denaturing two-dimensional electrophoresis after extraction with detergents2005

    • Author(s)
      島崎洋次, 真鍋 敬
    • Journal Title

      Biochim. Biophys. Acta 1749

      Pages: 95-101

    • Description
      「研究成果報告書概要(和文)」より
    • Data Source
      KAKENHI-PROJECT-16550077
  • [Journal Article] Detection of activity and mass spectrometric identification of mouse liver caboxylesterase and aldehyde dehydrogenase separated by non-denaturing two-dimensional electrophoresis after extraction with detergents2005

    • Author(s)
      Yoji Shimazaki, Takashi Manabe
    • Journal Title

      Biochim.Biophys.Acta 1749

      Pages: 95-101

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-16550077
  • [Journal Article] Nondenaturing Two-dimensional Electrophoresis Enzyme Profile Involving Activity and Sequence Structure of Cytosol Proteins from Mouse Liver2004

    • Author(s)
      島崎 洋次, 菅原 由貴, 真鍋 敬
    • Journal Title

      Proteomics 4・5

      Pages: 406-411

    • Data Source
      KAKENHI-PROJECT-16550077
  • [Journal Article] Nondenaturing Two-dimensional Electrophoresis Enzyme Profile Involving Activity and Sequence Structure of Cytosol Proteins from Mouse Liver2004

    • Author(s)
      島崎洋次, 菅原 由貴, 真鍋 敬
    • Journal Title

      Proteomics 4

      Pages: 1406-1411

    • Description
      「研究成果報告書概要(和文)」より
    • Data Source
      KAKENHI-PROJECT-16550077
  • [Journal Article] Simultaneous Analysis of Esterase and Transferase Activities in Cytosol Proteins from the Bovine Retina by Uning Microscale Non-Denaturing Two-Dimensional Electrophoresis2004

    • Author(s)
      Yoji Shimazaki, Yoko Hiraka, Minatsu Uesugi, Takashi Manabe
    • Journal Title

      Biochim.Biophys.Acta 1696

      Pages: 51-57

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-16550077
  • [Journal Article] Analysis of Esterase and Transferase Activities in Cytosol Proteins from the Bovine Retina by Uning Microscale Non-Denaturing Two-Dimensional Electrophoresis2004

    • Author(s)
      島崎洋次, 平加容子, 上杉美夏, 真鍋 敬
    • Journal Title

      Biochim. Biophys. Acta 1696

      Pages: 51-57

    • Description
      「研究成果報告書概要(和文)」より
    • Data Source
      KAKENHI-PROJECT-16550077
  • [Journal Article] Nondenaturing Two-dimensional Electrophoresis Enzyme Profile Involving Activity and Sequence Structure of Cytosol Proteins from Mouse Liver2004

    • Author(s)
      Yoji Shimazaki, Yuki Sugawara, Takashi Manabe
    • Journal Title

      Proteomics 4

      Pages: 1406-1411

    • Description
      「研究成果報告書概要(欧文)」より
    • Data Source
      KAKENHI-PROJECT-16550077
  • [Presentation] 私と電気泳動「電気泳動でタンパク質の機能を視る」2023

    • Author(s)
      島崎洋次,
    • Organizer
      第74回日本電気泳動学会学術集会
    • Invited
    • Data Source
      KAKENHI-PROJECT-21K05128
  • [Presentation] 第62回日本電気泳動学会 学会賞(児玉賞)受賞講演: 非変性条件の2次元電気泳動による天然酵素および複合体の分離と機能解析2023

    • Author(s)
      島崎洋次
    • Organizer
      第74回日本電気泳動学会学術集会
    • Invited
    • Data Source
      KAKENHI-PROJECT-21K05128
  • [Presentation] 非変性条件の2次元ゲル電気泳動法によるネイティブタンパク質の分離分析法の展望と課題2022

    • Author(s)
      島崎洋次, 福家麗
    • Organizer
      第72回日本電気泳動学会シンポジウム
    • Invited
    • Data Source
      KAKENHI-PROJECT-21K05128
  • [Presentation] 2次元ゲル電気泳動で分離されたタンパク質超分子の分析法の検討2022

    • Author(s)
      島崎洋次, 中尾香琳
    • Organizer
      日本分析化学会第71年会
    • Data Source
      KAKENHI-PROJECT-21K05128
  • [Presentation] 非変性条件の2次元電気泳動法で分離・検出されたタンパク質複合体の電気泳動法による抽出2022

    • Author(s)
      中尾香琳, 島崎洋次
    • Organizer
      第73回日本電気泳動学会
    • Data Source
      KAKENHI-PROJECT-21K05128
  • [Presentation] 等電点電気泳動分離溶出されたリゾチーム超分子の機能制御法の検討2021

    • Author(s)
      O島崎洋次、塚野鈴、籔隼大
    • Organizer
      日本分析化学会 第70年会
    • Data Source
      KAKENHI-PROJECT-21K05128
  • [Presentation] 非変性等電点電気泳動分離したタンパク質複合体の溶出法の検討とその特性分析2021

    • Author(s)
      O島崎洋次、籔隼大、塚野鈴
    • Organizer
      日本電気泳動学会第72回総会
    • Data Source
      KAKENHI-PROJECT-21K05128
  • [Presentation] 抗体膜法とMALDI-TOF MSによるアミロイドβ単量体の分離分析法の構築2015

    • Author(s)
      島崎洋次、鷹津陽子
    • Organizer
      日本分析化学会第64年会
    • Place of Presentation
      福岡伊都、九州大学
    • Year and Date
      2015-09-09
    • Data Source
      KAKENHI-PROJECT-25410145
  • [Presentation] 非変性条件の電気泳動法による酵素抗体複合体の分離法の検討2015

    • Author(s)
      島崎洋次、佐藤友紀、宮塚理乃
    • Organizer
      日本電気泳動学会
    • Place of Presentation
      東京、東京工科大学
    • Year and Date
      2015-09-04
    • Data Source
      KAKENHI-PROJECT-25410145
  • [Presentation] 生体酵素の機能と構造の分析法の開発2014

    • Author(s)
      島崎洋次
    • Organizer
      日本分析化学会中国四国支部愛媛地区講演会
    • Place of Presentation
      新居浜市市民文化センター (愛媛県新居浜市)
    • Year and Date
      2014-12-10
    • Invited
    • Data Source
      KAKENHI-PROJECT-25410145
  • [Presentation] 非変性条件の電気泳動後のタンパク質の機能解析法の検討

    • Author(s)
      島崎洋次、友澤孝宣
    • Organizer
      第65回日本電気泳動学会総会
    • Place of Presentation
      横浜情報文化センター (神奈川県横浜市)
    • Year and Date
      2014-10-24 – 2014-10-25
    • Data Source
      KAKENHI-PROJECT-25410145
  • [Presentation] アビジン固定化膜を用いた抗原選別と酵素消化連続分析法の確立

    • Author(s)
      島崎洋次、斉藤正樹
    • Organizer
      日本分析化学会 第62年会
    • Place of Presentation
      大阪
    • Data Source
      KAKENHI-PROJECT-25410145
  • [Presentation] アミロイドβ単量体および凝集体の分離分析法の検討

    • Author(s)
      島崎洋次、廣瀬優
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学 (広島県東広島市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Data Source
      KAKENHI-PROJECT-25410145
  • [Presentation] アミロイドβ1-42単量体の酵素分解による凝集化の制御法の検討

    • Author(s)
      斎藤正樹、島崎洋次
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学 (広島県東広島市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Data Source
      KAKENHI-PROJECT-25410145
  • 1.  MANABE Takashi (60094281)
    # of Collaborated Projects: 5 results
    # of Collaborated Products: 0 results
  • 2.  座古 保 (50399440)
    # of Collaborated Projects: 2 results
    # of Collaborated Products: 1 results
  • 3.  眞鍋 敬
    # of Collaborated Projects: 0 results
    # of Collaborated Products: 9 results

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* This action can be performed only by the researcher himself/herself who is listed on the KAKEN Researcher’s page. Are you sure that this KAKEN Researcher’s page is your page?

この研究者とORCID iDの連携を行いますか?
※ この処理は、研究者本人だけが実行できます。

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