Yamashita Kouwa 山下幸和

… Alternative Names

YAMASHITA Kouwa 山下幸和

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Researcher Number	80382670
Other IDs
Affiliation (Current)	2022: 横浜薬科大学, 薬学部, 特任教授
Affiliation (based on the past Project Information) *help	2015: 横浜薬科大学, 薬学部, 教授 2010 – 2014: 東北薬科大学, 薬学部, 教授
Review Section/Research Field	Principal Investigator Physical pharmacy Except Principal Investigator Physical pharmacy
Keywords	Principal Investigator LC-ESI-MS/MS / 乳がん / エストロゲン / オルトキノン / フェナジン誘導体 / 質量分析 / 分析化学 / エストロゲンオルトキノン / 定量分析 / LC-MS … More / ヘムタンパク / LC-ESI-MS/MS法 / targeted steroidomics / 副腎皮質ホルモン / 微量定量法 / プロトン親和性誘導体 / 鑑別診断法 / LC-ESI-MS法 / 臨床診断法 … More Except Principal Investigator ステロイド / LC-MS/MS Less

Targeted steroidomics research for diagnosis of adrenocortical diseasePrincipal Investigator
- Principal Investigator
  
  Yamashita Kouwa
- Project Period (FY)
  2013 – 2015
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Physical pharmacy
- Research Institution
  Yokohama College of Pharmacy
  Tohoku Pharmaceutical University
Metabolite profiling of biological steroids in pathological tissue specimen
- Principal Investigator
  
  HONDA Akira
- Project Period (FY)
  2011 – 2013
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Physical pharmacy
- Research Institution
  Tokyo Medical University
Development of the analytical method for estrogen-o-quinones and its application to the study on the initiation mechanism of human breast cancerPrincipal Investigator
- Principal Investigator
  
  YAMASHITA Kouwa
- Project Period (FY)
  2010 – 2012
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Physical pharmacy
- Research Institution
  Tohoku Pharmaceutical University

All 2016 2015 2014 2013 2012 2011 2010 Other

All Journal Article Presentation Book

[Book] Testosterone: Biochemistry, Therapeutic Uses and Physiological Effects2012
- Author(s)
  K. Yamashita (ed. by Konstantin Chichinadze)
- Total Pages
  193
- Publisher
  Nova Scientific Publishers Inc
- Data Source
  KAKENHI-PROJECT-22590041
[Book] Testosterone: Biochemistry, Therapeutic Uses and Physiological Effects2012
- Author(s)
  Kouwa Yamashita (ed. by Konstantin Chichinadze)
- Total Pages
  193
- Publisher
  Nova Scientific Publishers Inc
- Data Source
  KAKENHI-PROJECT-22590041
[Journal Article] Simultaneous quantification of salivary 3-hydroxybutyrate, 3-hydroxyisobutyrate, 3-hydroxy-3-methylbutyrate, and 2-hydroxybutyrate as a possible marker of amino acid and fatty acid catabolic pathways by LC-ESI-MS/MS2015
- Author(s)
  Miyazaki T, Honda A, Ikegami T, Iwamoto J, Monma T, Hirayama T, Saito Y, Yamashita K, Matsuzaki Y. . . .
- Journal Title
  
  SpringerPlus
  
  Volume: 4 Pages: 494-494
- DOI
  10.1186/s40064-015-1304-0
- Peer Reviewed / Acknowledgement Compliant / Open Access
- Data Source
  KAKENHI-PROJECT-15K00849, KAKENHI-PROJECT-25460042, KAKENHI-PROJECT-25461013, KAKENHI-PROJECT-25750334
[Journal Article] Measurement of peripheral plasma 18-oxocortisol can discriminate unilateral adenoma from bilateral diseases in patients with primary aldosteronism.2015
- Author(s)
  F. Satoh, R. Morimoto, Y. Ono, Y. Iwakura, K Omata, M. Kudo, K. Takase, K. Seiji, H. Sasamoto, S. Honma, M. Okuyama, K. Yamashita, CE.Gomez-Sanchez, WE. Rainey, Y. Arai, H. Sasano, Y. Nakamura, S. Ito
- Journal Title
  
  Hypertension.
  
  Volume: 65 Pages: 1096-1102
- DOI
  10.1161/hypertensionaha.114.04453
- Peer Reviewed / Open Access
- Data Source
  KAKENHI-PROJECT-25460042, KAKENHI-PROJECT-15H04711, KAKENHI-PROJECT-25462532
[Journal Article] Recernt developement of chemical derivatization in LC-MS for biomedical approaches2015
- Author(s)
  Ohno K, Yamashita K
- Journal Title
  
  Bioanalysis
  
  Volume: 7 Pages: 2489-2499
- DOI
  10.4155/bio.15.180
- Peer Reviewed / Acknowledgement Compliant
- Data Source
  KAKENHI-PROJECT-25460042
[Journal Article] An innovative LC-MS/MS-based method for determining CYP17 and CYP19 activity in the adipose tissue of pre- and postmenoposal and ovariectomized women using 13C-labeled steroid substrates2014
- Author(s)
  Toshihiko Kinoshita, Seijiro Honma, Yasuhiro Shibata, kouwa Yamashita, Yoko Watanabe, Hitoe Maekubo, Mitsunobu Okuyama, Akiko Takashima, Naoki Takeshita
- Journal Title
  
  J. Clin. Endocrinol. Metab.
  
  Volume: 99 Pages: 1339-1347
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-25460042
[Journal Article] Structures and Biomimetic Synthesis of Novel a-Pyrone Polyketide of an Endophytic Penicillium sp. Catharanthus roseus2013
- Author(s)
  Teigo Asai, Dan Luo, kouwa Yamashita, Yoshiteru Oshima
- Journal Title
  
  Organic Letters
  
  Volume: 15 Pages: 1021-1023
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22590041
[Journal Article] Structures and Biomimetic Synthesis of Polyketides of an Endophytic Penicillium sp. of Cataranthus roseus2013
- Author(s)
  T, Asai, D. Luo, K. Yamashita, Y. Oshima
- Journal Title
  
  Org. Letti
  
  Volume: 15 Pages: 1020-1023
- DOI
  10.1021/ol303506t
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22590041, KAKENHI-PROJECT-23651212, KAKENHI-PROJECT-23710248, KAKENHI-PROJECT-24590310
[Journal Article] 18-oxocortisol measurement in adrenal vein sampling as a biomarker for subclassifying primary aldosteronism.2011
- Author(s)
  Y. Nakamura, F. Satoh, R. Morimoto, M. Kudo, K. Takase, C E. Gometz-Sanchez, S. Honma, M. Okuyama, K. Yamashita, W E. Rainey, H. Sasano, S.Ito
- Journal Title
  
  J Clin Endocrinol Metab.
  
  Volume: 96
- DOI
  10.1210/jc.2010-2785
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22590041, KAKENHI-PROJECT-23390296
[Journal Article] Assay of labile estrogen-o-quinones, potent carcinogenic molecular species, by HPLC-electrosprayionization tandem mass spectrometry2010
- Author(s)
  Kouwa Yamashita
- Journal Title
  
  J.Steroid Biochem.Mol.Biol.
  
  Volume: 119 Pages: 141-148
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-22590041
[Journal Article] Assay of labile estrogen o-quinones, a potent carcinogenic molecular species, by high performance liquid chromatographyelectrospray ionization mass spectrometry with phenazine derivatization2010
- Author(s)
  K. Yamashita, A. Masuda, Y. Hoshino, S.Komatsu, M. Numazawa
- Journal Title
  
  J. Steroid Biochem. Mol. Biol
  
  Volume: 119 Pages: 141-148
- Data Source
  KAKENHI-PROJECT-22590041
[Presentation] LC-ESI-MS/MSによるコルチゾール生合成関連ステロイドの一斉定量2016
- Author(s)
  小松祥子、榊　教克、今　華恵、藤村　務、山下幸和
- Organizer
  日本薬学会第136年会
- Place of Presentation
  横浜（パシフィコ横浜）
- Year and Date
  2016-03-28
- Data Source
  KAKENHI-PROJECT-25460042
[Presentation] LC-ESI-MS/MSによる血清グルココルチコイドおよびその前駆体の一斉分析2015
- Author(s)
  小松祥子、齋藤彩夏、榊教克、今　華慧恵、齋藤　滋、藤村　務、山下幸和
- Organizer
  第54回日本薬学会東北支部大会
- Place of Presentation
  盛岡(岩手医科大学）
- Year and Date
  2015-09-26
- Data Source
  KAKENHI-PROJECT-25460042
[Presentation] 改良Marfey法を用いた微生物細胞内アミノ酸の検出法に関する研究2015
- Author(s)
  加藤　創、高橋和弘、原田健一、山下幸和、藤村　務
- Organizer
  第54回日本薬学会東北支部大会
- Place of Presentation
  盛岡(岩手医科大学）
- Year and Date
  2015-09-26
- Data Source
  KAKENHI-PROJECT-25460042
[Presentation] LC-ESI-MS/MS法による3b-HSD活性評価を目的とした基礎検討2014
- Author(s)
  小松祥子、榊　教克、山下幸和
- Organizer
  日本薬学会第134年会
- Place of Presentation
  熊本
- Data Source
  KAKENHI-PROJECT-25460042
[Presentation] 質量分析による生体試料中アンドロゲンの高感度分析法の開発2014
- Author(s)
  大野賢一、山下幸和
- Organizer
  第25回日本臨床化学会東北支部総会
- Place of Presentation
  盛岡（岩手医科大学）
- Year and Date
  2014-08-09
- Data Source
  KAKENHI-PROJECT-25460042
[Presentation] Aldosteroneのdimethylethylsilylおよびdimethylisopropylsilyl誘導体化とGC-MS分析2014
- Author(s)
  小松　祥子, 大池　裕貴, 山下幸和
- Organizer
  第53回日本薬学会東北支部大会
- Place of Presentation
  いわき（いわき明星大学）
- Year and Date
  2014-10-05
- Data Source
  KAKENHI-PROJECT-25460042
[Presentation] LC-ESI-MS/MS法による水酸化ステロイドのメタボローム解析(VII) ステロール類のピコリン酸およびN-メチルピペリジンカルボン酸誘導体の調製2013
- Author(s)
  小松祥子、都澤　優、千葉　淳、山崎敬子、山下幸和、沼澤光輝
- Organizer
  第52回日本薬学会東北支部大会
- Place of Presentation
  仙台
- Data Source
  KAKENHI-PROJECT-25460042
[Presentation] LC-ESI-MS/MS法による水酸化ステロイドのメタボローム解析(V)安定同位体標識カテコールエストロゲン－GSH付加体の合成－2013
- Author(s)
  山下幸和、近藤恵美、白取未希、小松祥子、加藤　創、大野賢一、沼澤光輝
- Organizer
  日本薬学会第133年会
- Place of Presentation
  横浜
- Data Source
  KAKENHI-PROJECT-22590041
[Presentation] LC-ESI-MS/MS法による水酸化ステロイドのメタボローム解析(VI)－ステロイド硫酸抱合体の定量法の最適化－2013
- Author(s)
  小松祥子、齋藤彩夏、榊　克教、山下幸和、沼澤光輝
- Organizer
  日本薬学会第133年会
- Place of Presentation
  横浜
- Data Source
  KAKENHI-PROJECT-22590041
[Presentation] Microdetermination of labile estrogen-o-quinones and their cysteine conjugates by high performance liquid chromatography-electrospray ionization tandem mass spectrometry2013
- Author(s)
  Kouwa Yamashita, Miki Shiratori, Emi Kondo, Sachiko Komatsu, Hajime Katoh, Ken-ichi Ohno
- Organizer
  The 39th International Symposium on High-Performance-Liquid-Phase Separation and Related Technique
- Place of Presentation
  Amsterdam, The Netherlands
- Data Source
  KAKENHI-PROJECT-25460042
[Presentation] アンドロゲンの高感度分析法開発と唾液試料への応用2013
- Author(s)
  田村智美、長谷川智美、大野賢一、山下幸和
- Organizer
  第52回日本薬学会東北支部大会
- Place of Presentation
  仙台
- Data Source
  KAKENHI-PROJECT-25460042
[Presentation] LC-ESI-MS/MS法による水酸化ステロイドのメタボローム解析(V)安定同位体標識カテコールエストロゲン-GSH 付加体の合成2013
- Author(s)
  山下幸和、近藤恵美、白取未希、小松祥子、加藤創、大野賢一、沼澤光輝
- Organizer
  日本薬学会第133 年会
- Place of Presentation
  横浜
- Year and Date
  2013-03-28
- Data Source
  KAKENHI-PROJECT-22590041
[Presentation] Pyridine-carboxylates as protonaffinitive derivatives in HPLC-ESI-MS/MS analysis of hydroxysteroids2012
- Author(s)
  K. Yamashita, S. Komatsu, H. Kato, K.-I.Ohno
- Organizer
  29^<th>LC/MS Montreux Symposium
- Place of Presentation
  Montreux
- Year and Date
  2012-11-09
- Data Source
  KAKENHI-PROJECT-22590041
[Presentation] LC-ESI-MS/MSによる水酸化ステロイドのメタボロム解析(IV)27 ステロイドの一斉分析2012
- Author(s)
  山下幸和、小松祥子、加藤創、大野賢一、沼澤光輝、本間誠次郎
- Organizer
  日本薬学会第132年会
- Place of Presentation
  札幌
- Year and Date
  2012-03-30
- Data Source
  KAKENHI-PROJECT-22590041
[Presentation] Use of proton-affinitive derivatization in HPLC-positive-ESI-MS analysis of biologically important carboxylic acids2012
- Author(s)
  Hajime Kato, Shin Mitsuzuka, Kouwa Yamashita, Mitsuteru Numazawa
- Organizer
  19th International Mass Spectrometry Conference
- Place of Presentation
  京都
- Data Source
  KAKENHI-PROJECT-22590041
[Presentation] Use of proton-affinitive derivatization in HPLC-ESI-MS analysis of biologically important carboxylic acids2012
- Author(s)
  H. Kato, S. Mitsuzuka, K. Yamashita, M. Numazawa
- Organizer
  19^<th>International Mass Spectrometry Conference
- Place of Presentation
  Kyoto
- Year and Date
  2012-09-20
- Data Source
  KAKENHI-PROJECT-22590041
[Presentation] LC-ESI-MS/MS によるアロマターゼ活性測定法の開発および従来法との比較2012
- Author(s)
  小松祥子、鈴木直樹、高橋透、山下幸和、沼澤光輝
- Organizer
  第51回日本薬学会東北支部大会
- Place of Presentation
  青森
- Year and Date
  2012-10-07
- Data Source
  KAKENHI-PROJECT-22590041
[Presentation] Pyridine-carboxylates as proton-affinitive derivatives in HPLC-ESI-MS/MS analysis of hydroxysteroids2012
- Author(s)
  Kouwa Yamashita, Sachiko Komatsu, Hajime Katoh, Ken-ichi Ohno
- Organizer
  29th LC/MS Montreux Symposium
- Place of Presentation
  Switzerland
- Data Source
  KAKENHI-PROJECT-22590041
[Presentation] LC-ESI-MS/MS法による水酸化ステロイドのメタボローム解析(IV)27ステロイドの一斉分析2012
- Author(s)
  山下幸和、小松祥子、加藤創、大野賢一、沼澤光輝、本間誠次郎
- Organizer
  日本薬学会第年会
- Place of Presentation
  札幌(北海道大学)
- Year and Date
  2012-03-30
- Data Source
  KAKENHI-PROJECT-22590041
[Presentation] LC-ESI-MS/MSによるアロマターゼ活性測定法の開発および従来法との比較2012
- Author(s)
  小松祥子、鈴木直樹、高橋　透、山下幸和、沼澤光輝
- Organizer
  第51回日本薬学会東北支部大会
- Place of Presentation
  青森
- Data Source
  KAKENHI-PROJECT-22590041
[Presentation] エストロゲンオルトキノンによるタンパク修飾(I)-L-Cys, N-Ac-L-Cys 及び GSH-共有結合体の調製とカテコール/キノン平衡2011
- Author(s)
  白取未希、加藤創、山下幸和、沼澤光輝
- Organizer
  第50回日本薬学会東北支部大会
- Place of Presentation
  仙台
- Year and Date
  2011-10-30
- Data Source
  KAKENHI-PROJECT-22590041
[Presentation] An approach to the microdetermination of labile estrogen-o-quinones by liquid chromatography-electrospray ionization tandem mass spectrometry2010
- Author(s)
  Kouwa Yamashita
- Organizer
  58^<th> ASMS Conference on Mass Spectrometry and Allied Topics
- Place of Presentation
  Salt Lake City, Utah USA
- Year and Date
  2010-05-24
- Data Source
  KAKENHI-PROJECT-22590041
[Presentation] An approach to the microdetermination of labile estrogen o-quinones by liquid chromatographyelectrospray ionization tandem mass spectrometry2010
- Author(s)
  K. Yamashita, S. Komatsu, A. Masuda, M. Numazawa
- Organizer
  58^<th>ASMS Confererence
- Place of Presentation
  Salt Lake City, USA
- Year and Date
  2010-05-25
- Data Source
  KAKENHI-PROJECT-22590041
[Presentation] コレステロールの前駆体および水酸化代謝物のLC-ESI-MS/MS分析
- Author(s)
  小松祥子, 千葉淳, 都澤優, 宮本和明, 山下幸和
- Organizer
  日本薬学会第135年会
- Place of Presentation
  神戸（兵庫医療大学ほか）
- Year and Date
  2015-03-26 – 2015-03-28
- Data Source
  KAKENHI-PROJECT-25460042
[Presentation] 胆汁酸のピコリルエステル化による高感度LC-MS分
- Author(s)
  加藤創, 三塚進, 加藤杏奈, 阿邊満夕子, 池田麻希, 山下幸和
- Organizer
  日本薬学会第135年会
- Place of Presentation
  神戸（兵庫医療大学ほか）
- Year and Date
  2015-03-26 – 2015-03-28
- Data Source
  KAKENHI-PROJECT-25460042

1. OHNO Ken-ichi (20347272)

# of Collaborated Projects: 2 results

# of Collaborated Products: 10 results
2. KATO Hajime (80584458)

# of Collaborated Projects: 2 results

# of Collaborated Products: 12 results
3. KOMATSU Sachiko (00438566)

# of Collaborated Projects: 2 results

# of Collaborated Products: 18 results
4. HONDA Akira (10468639)

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
5. MIYAZAKI Teruo (60532687)

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
6. 沼澤光輝 (90006338)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
7. SATOU Fumitoshi

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
8. SATOU Miho

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
9. IKEGAMI Tadashi

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
10. ONO Katsuhiko

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
11. 沼沢光輝

# of Collaborated Projects: 0 results

# of Collaborated Products: 12 results
12. 高瀬圭

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
13. 森本玲

# of Collaborated Projects: 0 results

# of Collaborated Products: 2 results
14. 岩本淳一

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
15. 松崎靖司

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
16. 荒井陽一

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
17. 中村保宏

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
18. 笹野公伸

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
19. Rainey William E

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
20. Gomez-Sanchez Celso E

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
21. 大島吉輝

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results
22. 浅井禎吾

# of Collaborated Projects: 0 results

# of Collaborated Products: 1 results

Yamashita Kouwa 山下 幸和

YAMASHITA Kouwa 山下 幸和

Research Projects

Research Products

Co-Researchers

Targeted steroidomics research for diagnosis of adrenocortical diseasePrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Metabolite profiling of biological steroids in pathological tissue specimen

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

Development of the analytical method for estrogen-o-quinones and its application to the study on the initiation mechanism of human breast cancerPrincipal Investigator

Principal Investigator

Project Period (FY)

Research Category

Research Field

Research Institution

[Book] Testosterone: Biochemistry, Therapeutic Uses and Physiological Effects2012

Author(s)

Total Pages

Publisher

Data Source

[Book] Testosterone: Biochemistry, Therapeutic Uses and Physiological Effects2012

Author(s)

Total Pages

Publisher

Data Source

[Journal Article] Simultaneous quantification of salivary 3-hydroxybutyrate, 3-hydroxyisobutyrate, 3-hydroxy-3-methylbutyrate, and 2-hydroxybutyrate as a possible marker of amino acid and fatty acid catabolic pathways by LC-ESI-MS/MS2015

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Measurement of peripheral plasma 18-oxocortisol can discriminate unilateral adenoma from bilateral diseases in patients with primary aldosteronism.2015

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Recernt developement of chemical derivatization in LC-MS for biomedical approaches2015

Author(s)

Journal Title

DOI

Data Source

[Journal Article] An innovative LC-MS/MS-based method for determining CYP17 and CYP19 activity in the adipose tissue of pre- and postmenoposal and ovariectomized women using 13C-labeled steroid substrates2014

Author(s)

Journal Title

Data Source

[Journal Article] Structures and Biomimetic Synthesis of Novel a-Pyrone Polyketide of an Endophytic Penicillium sp. Catharanthus roseus2013

Author(s)

Journal Title

Data Source

[Journal Article] Structures and Biomimetic Synthesis of Polyketides of an Endophytic Penicillium sp. of Cataranthus roseus2013

Author(s)

Journal Title

DOI

Data Source

[Journal Article] 18-oxocortisol measurement in adrenal vein sampling as a biomarker for subclassifying primary aldosteronism.2011

Author(s)

Journal Title

DOI

Data Source

[Journal Article] Assay of labile estrogen-o-quinones, potent carcinogenic molecular species, by HPLC-electrosprayionization tandem mass spectrometry2010

Author(s)

Journal Title

Data Source

[Journal Article] Assay of labile estrogen o-quinones, a potent carcinogenic molecular species, by high performance liquid chromatographyelectrospray ionization mass spectrometry with phenazine derivatization2010

Author(s)

Journal Title

Data Source

[Presentation] LC-ESI-MS/MSによるコルチゾール生合成関連ステロイドの一斉定量2016

Author(s)

Organizer

Place of Presentation

Year and Date

Data Source

Yamashita Kouwa 山下幸和

YAMASHITA Kouwa 山下幸和