Okuno Takashi 奥野貴士

… Alternative Names

Researcher Number	80411031
Other IDs
Affiliation (Current)	2021: 山形大学, 理学部, 准教授
Affiliation (based on the past Project Information) *help	2011 – 2019: 山形大学, 理学部, 准教授 2017: 山形大学, 大学院理工学研究科, 准教授 2010: 富山大学, 薬学部, 准教授 2008 – 2010: University of Toyama, 大学院・医学薬学研究部(薬学), 准教授
Review Section/Research Field	Principal Investigator Molecular biology / Biophysics / Bio-related chemistry / Complex systems / Basic Section 37010:Bio-related chemistry Except Principal Investigator Physical pharmacy / Functional biochemistry / Intelligent mechanics/Mechanical systems
Keywords	Principal Investigator 細胞膜 / 膜タンパク質 / 高速AFM / 細胞分裂 / ATPase / 分子間相互作用 / 膜タンパク質の複合体 / 平面脂質膜 / 膜分裂 / 細胞膜分裂 … More Except Principal Investigator 遺伝子送達 / 糖尿病 / インスリン / 皮膚 / AAAタンパク質 / 分子シャペロン / プロテアソーム / p97/VCP/CDC-48/Cdc48 / Katanin / 高速原子間力顕微鏡 / ミトコンドリア / 微小管 / katanin / p97/CDC-48/Cdc48 / ポリグルタミン凝集体 / 26Sプロテアソーム / 神経変性疾患 / AFMプローブ / 近接探針 / 磁歪薄膜 / 液中デリバリ / MEMS / 近接デュアル探針 / デュアルAFMプローブ / 中空カンチレバー / 磁歪薄膜アクチュエータ / 液体デリバリ Less

支持脂質膜電気泳動法による巨大膜タンパク質調製と高速AFMによる構造動態解析Principal Investigator
- Principal Investigator
  
  奥野貴士
- Project Period (FY)
  2018 – 2020
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Review Section
  
  Basic Section 37010:Bio-related chemistry
- Research Institution
  Yamagata University
Single molecule analysis of membrane protein in the cell membrane using new model cell membrane sheetPrincipal Investigator
- Principal Investigator
  
  Okuno Takashi
- Project Period (FY)
  2015 – 2017
- Research Category
  
  Grant-in-Aid for Scientific Research (C)
- Research Field
  
  Bio-related chemistry
- Research Institution
  Yamagata University
プロテアソームによるポリユビキチン化タンパク質分解過程の過渡的複合体の解析Principal Investigator
- Principal Investigator
  
  奥野貴士
- Project Period (FY)
  2015 – 2016
- Research Category
  
  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
- Review Section
  
  Complex systems
- Research Institution
  Yamagata University
Magneto-strictive-driven dual AFM probe for narrow-gapped delivery in liquid
- Principal Investigator
  
  Mineta Takashi
- Project Period (FY)
  2015 – 2017
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Intelligent mechanics/Mechanical systems
- Research Institution
  Yamagata University
Molecular mechanisms of AAA proteins revealed by biochemical analyses and high-speed atomic force microscopic observations
- Principal Investigator
  
  OGURA Teru
- Project Period (FY)
  2012 – 2014
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Functional biochemistry
- Research Institution
  Kumamoto University
Development of novel type of flat lipid membrane for observation of complex formation process and structural analysis of divisomePrincipal Investigator
- Principal Investigator
  
  OKUNO Takashi
- Project Period (FY)
  2011 – 2013
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Biophysics
- Research Institution
  Yamagata University
Development of a noninvasive transdermal gene delivery system for gene therapy of diabetes
- Principal Investigator
  
  KOGURE Kentaro
- Project Period (FY)
  2008 – 2010
- Research Category
  
  Grant-in-Aid for Scientific Research (B)
- Research Field
  
  Physical pharmacy
- Research Institution
  Kyoto Pharmaceutical University
Analysis of proteins regulating bacterial cell division sitePrincipal Investigator
- Principal Investigator
  
  OKUNO Takashi
- Project Period (FY)
  2008 – 2009
- Research Category
  
  Grant-in-Aid for Young Scientists (B)
- Research Field
  
  Molecular biology
- Research Institution
  University of Toyama

All 2019 2017 2016 2015 2014 2013 2012 2010 2009 2008 Other

All Journal Article Presentation Book Patent

[Book] Subcell. Biochem2013
- Author(s)
  Okuno, T., and Ogura, T.
- Total Pages
  17
- Publisher
  Springer
- Data Source
  KAKENHI-PROJECT-24370056
[Book] FtsH Protease-Mediated Regulation of Various Cellular Functions2013
- Author(s)
  Okuno T., Ogura T.
- Publisher
  Subcell Biochem
- Data Source
  KAKENHI-PROJECT-23770173
[Book] Handbook of Proteolytic Enzymes, 3rd edition2013
- Author(s)
  Ogura, T., Okuno, T., Suno, R., and Akiyama, Y.
- Total Pages
  8
- Publisher
  Elsevier Ltd.
- Data Source
  KAKENHI-PROJECT-24370056
[Book] FtsH protease-mediated regulation of various cellular functions. Subcell.Biochem, 66, Regulated proteolysis in microorganisms. D. A. Dougan (ed.)2013
- Author(s)
  Okuno, T., and Ogura, T.
- Total Pages
  17
- Publisher
  Springer
- Data Source
  KAKENHI-PROJECT-24370056
[Book] Regulated proteolysis in microorganisms.2013
- Author(s)
  Okuno, T., and Ogura, T.
- Total Pages
  8
- Publisher
  FtsH protease-mediated regulation of various cellular functions.
- Data Source
  KAKENHI-PROJECT-23770173
[Book] FtsH protease. In Handbook of Proteolytic Enzymes, 3rd edition, N. Rawlings andG. Salvesen (ed.)2013
- Author(s)
  Ogura, T., Okuno, T., Suno, R., and Akiyama, Y.
- Total Pages
  8
- Publisher
  Elsevier Ltd.
- Data Source
  KAKENHI-PROJECT-24370056
[Journal Article] Structural Analysis of Streptococcus pyogenes NADH Oxidase: Conformational Dynamics Involved in Formation of the C(4a)-Peroxyflavin Intermediate.2015
- Author(s)
  Wallen JR, Mallett TC, Okuno T, Parsonage D, Sakai H, Tsukihara T, Claiborne A.
- Journal Title
  
  Biochemistry
  
  Volume: 54 Pages: 6815-6829
- DOI
  10.1021/acs.biochem.5b00676
- Peer Reviewed / Int'l Joint Research
- Data Source
  KAKENHI-PROJECT-15K05556
[Journal Article] Comparison of intracellular localization between Nubpl and Nubp2 utilizing GFP fusion protein.2010
- Author(s)
  Okuno T., Yamabayashi H., Kogure K.
- Journal Title
  
  Mol.Biol.Rep. 37
  
  Pages: 1165-1168
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20770142
[Journal Article] Comparison of intracellular localization between Nubp1 and Nubp2 utilizing GFP fusion protein.2010
- Author(s)
  Okuno T., Yamabayashi H., Kogure K.
- Journal Title
  
  Mol. Biol. Rep. 37
  
  Pages: 1165-1168
- Data Source
  KAKENHI-PROJECT-20770142
[Journal Article] Direct monitoring of interaction between Escherichia coli proteins., MinC and monomeric FtsZ, in solution.2009
- Author(s)
  Okuno T., Ogoh M., Tanina H., Funasaki N., Kogure K.
- Journal Title
  
  Biol. Pharm. Bull. 32
  
  Pages: 1473-1475
- NAID
  130000117267
- Data Source
  KAKENHI-PROJECT-20770142
[Journal Article] Direct monitoring of interaction between Escherichia coli proteins, MinC and monomeric FtsZ, in solution.2009
- Author(s)
  Okuno T., Ogoh M., Tanina H ., Funasaki N., Kogure K.
- Journal Title
  
  Biol.Pharm.Bull. 32
  
  Pages: 1473-1475
- NAID
  130000117267
- Peer Reviewed
- Data Source
  KAKENHI-PROJECT-20770142
[Patent] 動物細胞から細胞膜を切り出す方法、及び切り出された細胞膜2013
- Inventor(s)
  奥野貴士、南祐太、小野寺美紀
- Industrial Property Rights Holder
  国立大学法人山形大学
- Industrial Property Rights Type
  特許
- Industrial Property Number
  2013-228766
- Filing Date
  2013-11-01
- Data Source
  KAKENHI-PROJECT-23770173
[Presentation] ヒト培養細胞から基板支持細胞膜を直接作る研究2019
- Author(s)
  土田修平, 鈴木琢斗, 奥野貴士
- Organizer
  日本生物物理学会東北支部会
- Data Source
  KAKENHI-PROJECT-18K05311
[Presentation] Development of micro-patterned quartz and cell membrane hybrid device for monitoring of molecular transport via cell membrane2017
- Author(s)
  坂本知由子,中谷博幸,坂根礼文,南祐太,奥野貴士
- Organizer
  第39回分子生物学会年会
- Data Source
  KAKENHI-PROJECT-15K05556
[Presentation] 細胞膜機能解析へ向けた細胞膜試料マニピュレーション2017
- Author(s)
  坂本知由子,中谷博幸,坂根礼文,奥野貴士
- Organizer
  第12回トランスポーター研究会年会
- Data Source
  KAKENHI-PROJECT-15K05556
[Presentation] 細胞膜を介した物質輸送評価のための微細加工石英-細胞膜ハイブリッドデバイス開発2017
- Author(s)
  坂本知由子,中谷博幸,坂根礼文,南祐太,奥野貴士
- Organizer
  第11回トランスポーター研究会年会
- Data Source
  KAKENHI-PROJECT-15K05556
[Presentation] アクチンフィラメントの細胞膜シート上への結合2016
- Author(s)
  Shun Wakamatsu, Kuniyuki Hatori, Takashi Okuno
- Organizer
  第５４回生物物理学会年会
- Place of Presentation
  つくば国際会議場（茨城県・つくば市）
- Year and Date
  2016-11-25
- Data Source
  KAKENHI-PROJECT-15K05556
[Presentation] 細胞膜を介した物質輸送評価のための微細加工石英-細胞膜ハイブリッドデバイス開発2016
- Author(s)
  坂本知由子、中谷博幸、坂根礼文、南祐太、奥野貴士
- Organizer
  第３９回分子生物学会年会
- Place of Presentation
  パシフィコ横浜（神奈川県・横浜市）
- Year and Date
  2016-11-30
- Data Source
  KAKENHI-PROJECT-15K05556
[Presentation] 細胞膜を介した物質輸送評価のための微細加工石英-細胞膜ハイブリッドデバイス開発2016
- Author(s)
  坂本知由子、中谷博幸、坂根礼文、南祐太、奥野貴士
- Organizer
  第１１回トランスポーター研究会年会
- Place of Presentation
  京都大学きはだホール（京都府・宇治市）
- Year and Date
  2016-07-02
- Data Source
  KAKENHI-PROJECT-15K05556
[Presentation] 培養細胞から膜機能を維持したまま細胞膜を切り出す方法2016
- Author(s)
  川畑智美、奥野貴士
- Organizer
  第１１回トランスポーター研究会年会
- Place of Presentation
  京都大学きはだホール（京都府・宇治市）
- Year and Date
  2016-07-02
- Data Source
  KAKENHI-PROJECT-15K05556
[Presentation] ヒト培養細胞由来の巨大細胞膜小胞の細胞膜モデルの開発2015
- Author(s)
  奥野貴士、南祐太、飯沼博章、川畑智美、佐藤啓史、松村俊希、若松駿
- Organizer
  第４２回生体分子科学討論会
- Place of Presentation
  高崎市
- Year and Date
  2015-06-12
- Data Source
  KAKENHI-PROJECT-15K05556
[Presentation] Development of new device for manipulation of giant plasma membrane vesicles2015
- Author(s)
  佐藤啓史、坂根礼文、中谷隆、奥野貴士
- Organizer
  第５３回日本生物物理学会
- Place of Presentation
  金沢市
- Year and Date
  2015-09-13
- Data Source
  KAKENHI-PROJECT-15K05556
[Presentation] 新しい巨大細胞膜小胞の作製方法とその基板支持膜の物性2015
- Author(s)
  奥野貴士、南祐太、飯沼博章、川畑智美、佐藤啓史、松村俊希、若松駿
- Organizer
  日本膜学会第３７年会
- Place of Presentation
  早稲田大学
- Year and Date
  2015-05-14
- Data Source
  KAKENHI-PROJECT-15K05556
[Presentation] Morphology change of giant plasma membrane vesicles induced by nucleotides2015
- Author(s)
  若松駿、奥野貴士
- Organizer
  第５３回日本生物物理学会
- Place of Presentation
  金沢市
- Year and Date
  2015-09-13
- Data Source
  KAKENHI-PROJECT-15K05556
[Presentation] ２６Sプロテアソームの高速AFM観察2015
- Author(s)
  奥野貴士、野井健太郎、有田（森岡）健一、土屋光、佐伯泰、高橋一暢、伊野部智由、斎藤貴洋、山中邦俊、小椋光
- Organizer
  第５３回日本生物物理学会年会
- Place of Presentation
  金沢市
- Year and Date
  2015-09-13
- Data Source
  KAKENHI-PUBLICLY-15H01627
[Presentation] High-speed AFM imaging of the 26S proteasome dynamics.2014
- Author(s)
  Okuno, T., Noi, K., Okawa, A., Tsuchiya, H., Saeki, Y., Takahashi, K., Inobe, T., Yamanaka, K., and Ogura, T.
- Organizer
  231st IMEG seminars & Minisymposium
- Place of Presentation
  熊本市　熊本大学発生医学研究所
- Data Source
  KAKENHI-PROJECT-24370056
[Presentation] 細胞膜を試験管に取り出す方法2013
- Author(s)
  川畑智美,南祐太,櫻井敏彦,奥野貴士
- Organizer
  細胞を創る研究会6.0
- Place of Presentation
  鶴岡
- Data Source
  KAKENHI-PROJECT-23770173
[Presentation] 高速AFMによる26Sプロテアソームのダイナミクス解析.2013
- Author(s)
  奥野貴士, 野井健太郎, 大川阿紀子, 土屋光, 佐伯泰, 高橋一暢, 伊野部智由, 山中邦俊, 小椋光
- Organizer
  第36回日本分子生物学会年会
- Place of Presentation
  神戸市、神戸国際展示場
- Data Source
  KAKENHI-PROJECT-24370056
[Presentation] 膜タンパク質解析のためのモデル膜開発：細胞膜を切出してガラス基板に展開した平面膜構造体2013
- Author(s)
  南祐太,飯沼博章,小野寺美紀,櫻井敏彦,奥野貴士
- Organizer
  日本膜学会年会
- Place of Presentation
  早稲田大学西早稲田キャンパス（東京）
- Data Source
  KAKENHI-PROJECT-23770173
[Presentation] 細胞膜を試験管に取り出す方法2013
- Author(s)
  川畑智美,南祐太,櫻井敏彦,奥野貴士
- Organizer
  細胞を創る研究会6.0
- Place of Presentation
  慶應義塾大学鶴岡タウンキャンパス（鶴岡）
- Data Source
  KAKENHI-PROJECT-23770173
[Presentation] Dynamics of p97 and the 26S proteasome in action revealed by high-speed atomic force microscopy.2013
- Author(s)
  Ogura, T., Noi, K., Okuno, T., Arita-Morioka, K., Yamamoto, D., Okawa, A., Tsuchiya, H., Saeki, Y., Ando, T., Esaki, M., and Yamanaka, K.
- Organizer
  EMBO Workshop on AAA+ Proteins
- Place of Presentation
  Commundo Hotel, Neuss, Germany.
- Invited
- Data Source
  KAKENHI-PROJECT-24370056
[Presentation] GPMVs由来細胞膜シートの物性評価と微細加工基板によるマニピレーション技術2013
- Author(s)
  南祐太,飯沼博章,櫻井敏彦,奥野貴士
- Organizer
  細胞を創る研究会6.0
- Place of Presentation
  鶴岡
- Data Source
  KAKENHI-PROJECT-23770173
[Presentation] Development of a new method for preparation of cell membrane flat sheet on glass surface2013
- Author(s)
  南祐太,飯沼博章,櫻井敏彦,奥野貴士
- Organizer
  第51回日本生物物理学会年会
- Place of Presentation
  京都
- Data Source
  KAKENHI-PROJECT-23770173
[Presentation] GPMVs由来細胞膜シートの物性評価と微細加工基板によるマニピレーション技術2013
- Author(s)
  南祐太,飯沼博章,櫻井敏彦,奥野貴士
- Organizer
  細胞を創る研究会6.0
- Place of Presentation
  慶應義塾大学鶴岡タウンキャンパス（鶴岡）
- Data Source
  KAKENHI-PROJECT-23770173
[Presentation] Development of a new method for preparation of cell membrane flat sheet on glass surface2013
- Author(s)
  南祐太,飯沼博章,櫻井敏彦,奥野貴士
- Organizer
  第５１回日本生物物理学会年会
- Place of Presentation
  国立京都国際会館（京都）
- Data Source
  KAKENHI-PROJECT-23770173
[Presentation] Development of method for preparation of giant plasma membrane vesicles using surfactants2013
- Author(s)
  飯沼博章,南祐太,櫻井敏彦,奥野貴士
- Organizer
  第51回日本生物物理学会年会
- Place of Presentation
  京都
- Data Source
  KAKENHI-PROJECT-23770173
[Presentation] Development of method for preparation of giant plasma membrane vesicles using surfactants2013
- Author(s)
  飯沼博章,南祐太,櫻井敏彦,奥野貴士
- Organizer
  第５１回日本生物物理学会年会
- Place of Presentation
  国立京都国際会館（京都）
- Data Source
  KAKENHI-PROJECT-23770173
[Presentation] ベシクル状に切り出した細胞膜を基板上に展開する研究2012
- Author(s)
  南祐太,飯沼博章,櫻井敏彦,奥野貴士
- Organizer
  細胞を創る研究会5.0
- Place of Presentation
  東京
- Data Source
  KAKENHI-PROJECT-23770173
[Presentation] 界面活性物質を用いて細胞膜をベシクル状に切り出す研究2012
- Author(s)
  飯沼博章,南祐太,菅原有希,上野雅晴,櫻井敏彦,奥野貴士
- Organizer
  細胞を創る研究会5.0
- Place of Presentation
  東京
- Data Source
  KAKENHI-PROJECT-23770173
[Presentation] 新規の平面脂質膜構造体の膜タンパク質機能のイメージングへの応用2009
- Author(s)
  奥野貴士, 小暮健太朗, 上野雅晴
- Organizer
  日本薬学会北陸支部121回例会
- Place of Presentation
  富山
- Year and Date
  2009-12-05
- Data Source
  KAKENHI-PROJECT-20770142
[Presentation] 新規の平面脂質膜構造体の膜タンパク質機能のイメージングへの応用2009
- Author(s)
  奥野貴士, 小暮健太朗, 上野雅晴
- Organizer
  日本薬学会北陸支部12回例会
- Place of Presentation
  富山市
- Year and Date
  2009-12-05
- Data Source
  KAKENHI-PROJECT-20770142
[Presentation] Comparison of intracellular localization of Nubp 1 and Nubp2 using GFP fusion proteins.2009
- Author(s)
  Okuno T., Yamabayashi H., Kogure K., Ueno M.
- Organizer
  日本分子生物学会第32回年会
- Place of Presentation
  横浜市
- Year and Date
  2009-12-10
- Data Source
  KAKENHI-PROJECT-20770142
[Presentation] Comparison of intracellular localization of Nubp1 and Nubp2 using GFP fusion proteins.2009
- Author(s)
  Okuno T., Yamabayashi H., Kogure K., Ueno M.
- Organizer
  日本分子生物学会第32回年会
- Place of Presentation
  横浜
- Year and Date
  2009-12-10
- Data Source
  KAKENHI-PROJECT-20770142
[Presentation] Analysis of interaction between MinC and FtsZ by FRET.2008
- Author(s)
  奥野貴士, 丸山真由美, 小郷真智子, 谷名宏允, 舟崎紀昭, 小暮健太朗
- Organizer
  日本生物物理学会第46回年会
- Place of Presentation
  福岡
- Year and Date
  2008-12-03
- Data Source
  KAKENHI-PROJECT-20770142
[Presentation] FRETを利用したバクテリア分裂制御タンパク質間の相互作用の解析2008
- Author(s)
  奥野貴士, 小郷真智子, 谷名宏允, 舟崎紀昭, 小暮健太朗
- Organizer
  第58回日本薬学会近畿支部大会
- Place of Presentation
  神戸
- Year and Date
  2008-10-25
- Data Source
  KAKENHI-PROJECT-20770142
[Presentation] Analysis of interaction between MinC and FtsZ by FRET2008
- Author(s)
  奥野貴士, 丸山真由美, 小郷真智子, 谷名宏允, 舟崎紀昭, 小暮健太朗
- Organizer
  日本生物物理学会第46回年会
- Place of Presentation
  福岡
- Data Source
  KAKENHI-PROJECT-20770142
[Presentation] FRETを利用したバクテリア分裂制御タンパク質問の相互作用の解析2008
- Author(s)
  奥野貴士, 小郷真智子, 谷名宏允, 舟崎紀昭, 小暮健太朗
- Organizer
  第58回日本薬学会近畿支部大会
- Place of Presentation
  神戸
- Year and Date
  2008-10-25
- Data Source
  KAKENHI-PROJECT-20770142
[Presentation] Visualization of dynamics of the 26S proteasome and proteasome-substrate complexes by high-speed AFM
- Author(s)
  Okuno, T., Noi, K., Okawa, A., Tsuchiya, H., Saeki, Y., Takahashi, K., Inobe, T., Yamanaka, K., and Ogura, T.
- Organizer
  Key Forum: From Stem Cells to Organs
- Place of Presentation
  熊本市　熊本市医師会館
- Year and Date
  2014-09-04 – 2014-09-05
- Data Source
  KAKENHI-PROJECT-24370056

1. KOGURE Kentaro (70262540)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
2. OGURA Teru (00158825)

# of Collaborated Projects: 1 results

# of Collaborated Products: 8 results
3. YAMANAKA Kunitoshi (90212290)

# of Collaborated Projects: 1 results

# of Collaborated Products: 4 results
4. ESAKI Masatoshi (70437911)

# of Collaborated Projects: 1 results

# of Collaborated Products: 1 results
5. Mineta Takashi (50374814)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
6. SHIBATA Takayuki (10235575)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
7. 櫻井敏彦 (10332868)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results
8. 小池邦博 (40241723)

# of Collaborated Projects: 1 results

# of Collaborated Products: 0 results

Okuno Takashi 奥野 貴士

OKUNO Takashi 奥野 貴士

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支持脂質膜電気泳動法による巨大膜タンパク質調製と高速AFMによる構造動態解析Principal Investigator

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Single molecule analysis of membrane protein in the cell membrane using new model cell membrane sheetPrincipal Investigator

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プロテアソームによるポリユビキチン化タンパク質分解過程の過渡的複合体の解析Principal Investigator

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Magneto-strictive-driven dual AFM probe for narrow-gapped delivery in liquid

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Molecular mechanisms of AAA proteins revealed by biochemical analyses and high-speed atomic force microscopic observations

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Development of novel type of flat lipid membrane for observation of complex formation process and structural analysis of divisomePrincipal Investigator

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Development of a noninvasive transdermal gene delivery system for gene therapy of diabetes

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Analysis of proteins regulating bacterial cell division sitePrincipal Investigator

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[Book] Subcell. Biochem2013

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[Book] FtsH Protease-Mediated Regulation of Various Cellular Functions2013

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[Book] Handbook of Proteolytic Enzymes, 3rd edition2013

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[Book] FtsH protease-mediated regulation of various cellular functions. Subcell.Biochem, 66, Regulated proteolysis in microorganisms. D. A. Dougan (ed.)2013

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[Book] Regulated proteolysis in microorganisms.2013

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[Book] FtsH protease. In Handbook of Proteolytic Enzymes, 3rd edition, N. Rawlings andG. Salvesen (ed.)2013

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Okuno Takashi 奥野貴士

OKUNO Takashi 奥野貴士